Mengyuan Li¹, Zhongye Gao^1,2, Lina Jiang¹, Leishan Chen^1,2,*, Jianhui Ma^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 329-342, 2024, DOI:10.32604/phyton.2024.046976

Abstract Abscisic acid (ABA), hydrogen peroxide (H₂O₂) and ascorbate (AsA)–glutathione (GSH) cycle are widely known for their participation in various stresses. However, the relationship between ABA and H₂O₂ levels and the AsA–GSH cycle under drought stress in wheat has not been studied. In this study, a hydroponic experiment was conducted in wheat seedlings subjected to 15% polyethylene glycol (PEG) 6000–induced dehydration. Drought stress caused the rapid accumulation of endogenous ABA and H₂O₂ and significantly decreased the number of root tips compared with the control. The application of ABA significantly increased the number of root tips, whereas the application of H₂O₂ markedly… More >

Jie Zhang¹, Yingwei Zhao², Hongying Li³, Jianwei Ni^4,*, Dongmei Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2591-2610, 2023, DOI:10.32604/phyton.2023.028338

Abstract Paper mulberry is a high-quality woody feed resource plant with high crude protein content. It is widely distributed in China and has excellent characteristics of salt and alkali tolerance. Paper mulberry has ecological and economic importance. Salt stress has become a critical factor with the increasing degree of soil salinity that restricts plant growth. In the saline-alkali environments, transcriptome expression is altered leading to phenotypic defects in most plants. However, the regulatory mechanism related to paper mulberry’s salt-stress (SS) response is not clearly understood. In the present study de novo transcriptomic assembly was performed, and gene expression levels were measured… More >

Yue Jin¹, Lulu Zhi¹, Xin Tang¹, Yilin Chen¹, John T. Hancock², Xiangyang Hu^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2211-2225, 2023, DOI:10.32604/phyton.2023.026595

Abstract Gamma-aminobutyric acid (GABA) is a ubiquitous four-carbon non-protein amino acid that is involved in various physiological processes of plant growth and development, such as root architecture, stem elongation, leaf senescence, pollen tube growth, fruit ripening, and seed germination. GABA is also related to plant stress responses, such as drought, salt, cold, and heat stresses. Regulation of GABA in plant stress responses is complex and involves multiple signaling pathways, including calcium and hormone signaling. This paper systematically reviews the synthesis, metabolic pathways and regulatory role of GABA in plants, which will provide new insights into the understanding of plant growth and… More >

Sang Ik Song^*

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2017-2034, 2023, DOI:10.32604/phyton.2023.027469

Abstract Small ubiquitin-like modifier (SUMO) E3 ligases that facilitate the conjugation of SUMO proteins to target substrates contain an SP-RING domain which is like the RING domain found in ubiquitin E3 ligases. In this study, we isolated and characterized the Oryza sativa protein inhibitor of activated STAT like1 (OsPIAL1) containing SP-RING domains, as the rice homolog of Arabidopsis PIALs. OsPIAL1 interacts with OsSUMO proteins but does not interact with rice SUMO-conjugating enzymes (OsSCEs). An analysis of transgenic rice plant shows that OsPIAL1 is involved in SUMO conjugation to SCEs but not in SUMO conjugation to substrates. In addition, this OsPIAL1 activity… More >

XIAOHONG FU^1,#, JIE ZHAO^3,#, DANDAN CAO^1,2, CHENGXING HE^1,2, ZIYI WANG¹, YIBEI JIANG¹, JIANFENG LIU^1,*, GUIXIA LIU^1,*

BIOCELL, Vol.47, No.4, pp. 905-917, 2023, DOI:10.32604/biocell.2023.026930

Abstract Allium senescens, is an important economic and ecological grassland plant with drought-resistant characteristics. A TCP protein transcription factor is important in the regulation of plant development and adverse responses. However, the mechanism by which TCP transcription functions in drought resistance in Allium senescens is still not clear. Here, we obtained a total of 190,305 transcripts with 115,562 single gene clusters based on RNA-Seq sequencing of Allium senescens under drought stress. The total number of bases was 97,195,096 bp, and the average length was 841.06 bp. Furthermore, we found that there were eight genes of the TCP family that showed an… More >

Di Wang^1,#,*, Hao Gao^1,#, Jian Wang¹, Baoshan Cheng¹, Gang Li¹, Weijun Xu¹, Xi Liu^2,3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1173-1183, 2023, DOI:10.32604/phyton.2023.026526

Abstract Although tocopherols are essential for rice development, the molecular details by which their absence affects development remain to be determined. To study how tocopherols function during rice development, we performed a transcriptome deep sequencing (RNA-seq) analysis of the rice cultivar Nipponbare (Nip) and the tocopherol-deficient mutant small grain and dwarf 1-2 (sgd1-2). We identified 563 differentially expressed genes that were enriched in Gene Ontology categories associated with metabolism, stress, cellular responses, and transcriptional regulation. We determined that the total fatty acid composition of Nip and sgd1-2 was comparable, although cell membrane penetrability in sgd1-2 was significantly higher than in Nip… More >

Bingsong Yang^1,2,#, Xiaomeng Shi^2,#, Tingting Guo^1,2, Meixia Liang^1,2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 559-575, 2023, DOI:10.32604/phyton.2022.024266

Abstract Low temperature is one of the adversities threatening the growth and development and reduces the yield of rubber trees. However, molecular mechanisms toward rubber trees in response to low temperature are largely unclear. In this study, 7,159 and 7,600 differentially expressed genes (DEGs) were identified in ‘Reyan 73397’ rubber trees. Through GO analysis, the catalytic activity was the representative of the GO term in the only DEGs at the two studied temperatures (room temperature and 4°C, respectively), while KEGG analysis showed that carbon metabolism was the most important grouping under the comparison of these two temperatures. In addition, expression of… More >

JINXIAN LIU¹, JINGFANG FENG², CHANG ZHANG², YONGJUAN REN², WEIHUA SU², GUANGHENG WU¹, XIANYU FU¹, NING HUANG³, YOUXIONG QUE², HUI LING^3,*, JUN LUO^2,*

BIOCELL, Vol.47, No.1, pp. 215-225, 2023, DOI:10.32604/biocell.2022.022477

Abstract Calmodulin (CaM) proteins play a key role in signal transduction under various stresses. In the present study, the effects of a sugarcane ScCaM gene (NCBI accession number: GQ246454) on drought and salt stress tolerance in transgenic Arabidopsis thaliana and Escherichia coli cells were evaluated. The results demonstrated a significant negative role of ScCaM in the drought and salt stress tolerance of transgenic lines of A. thaliana, as indicated by the phenotypes. In addition, the expression of AtP5CS and AtRD29A, two genes tightly related to stress resistance, was significantly lower in the overexpression lines than in the wild type. The growth… More >

Wenting Wu¹ , Yajing Zhang¹, Yu Gao¹, Kai Zhang^2,3, Luying Zhu^1,3,*, Hongxia Zhang^2,3

Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1629-1642, 2022, DOI:10.32604/phyton.2022.020515

Abstract Leymus mollis, a wild relative of wheat, is very tolerant to salt stress, and has been considered as a valuable genetic resource for wheat breeding. However, the genetic basis for salt tolerance of this species is still largely unknown. In this study, de novo sequencing, assembly and analysis of L. mollis transcriptome in response to salt stress was performed. A total of 110,323 and 112,846 unigenes were generated for the NaCl-free (CK) and 180 mM NaCl-treated (CT) library, respectively. For the two libraries, 73,414 unigenes were successfully annotated in five common protein databases, and 7521 differentially expressed genes (DEGs) between… More >

Fenni Lv^1,2,#, Sen Wang^3,#, Ruiping Tian¹, Peng Wang², Kang Liu^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1645-1660, 2021, DOI:10.32604/phyton.2021.016947

Abstract Villin (VLN) is considered to be one of the most important actin-binding proteins, participates in modulating the actin cytoskeleton dynamics, plays essential role in plant development and resisting adverse environments. However, systematic studies of the VLN gene family have not been reported in cotton (Gossypium). In this study, 14 GhVLNs were identified in G. hirsutum. These GhVLN genes were distributed in 6 A-subgenome chromosomes and 6 D-subgenome chromosomes of the allotetraploid upland cotton and classified into three phylogenetical groups based on the classification model of AtVLNs. In addition, the 14 GhVLN genes have highly conserved gene structure and motif architecture.… More >