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  • Open Access

    ARTICLE

    Effects of Drought Stress on the Physiological Characteristics of Flue-Cured Tobacco during the Vigorous Growing Period

    Kesu Wei1, Guangju Liu2, Bin Wei3, Qifang Zhang4, Shengjiang Wu1, Zhaowei Li2,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1287-1298, 2025, DOI:10.32604/phyton.2025.062385 - 30 April 2025

    Abstract To systematically examine the tolerance of flue-cured tobacco K326 to soil drought stress, this study set up different water content trials for K326 at 60%, 40%, and 20% levels during the vigorous growing period by greenhouse pot planting. Pigment content, chlorophyll fluorescence parameters, antioxidant enzyme activity, and SPS and GS activities associated with carbon and nitrogen metabolism were investigated in the K326 tobacco leaves. The results showed that chlorophyll a and chlorophyll b contents decreased, non-photochemical quenching coefficient Y(NPQ) decreased in the later stage of vigorous growth, and energy dissipation quantum yield Y(NO) levels increased… More >

  • Open Access

    ARTICLE

    External Knowledge-Enhanced Cross-Attention Fusion Model for Tobacco Sentiment Analysis

    Lihua Xie1, Ni Tang1, Qing Chen1,*, Jun Li2,*

    CMC-Computers, Materials & Continua, Vol.82, No.2, pp. 3381-3397, 2025, DOI:10.32604/cmc.2024.058950 - 17 February 2025

    Abstract In the age of information explosion and artificial intelligence, sentiment analysis tailored for the tobacco industry has emerged as a pivotal avenue for cigarette manufacturers to enhance their tobacco products. Existing solutions have primarily focused on intrinsic features within consumer reviews and achieved significant progress through deep feature extraction models. However, they still face these two key limitations: (1) neglecting the influence of fundamental tobacco information on analyzing the sentiment inclination of consumer reviews, resulting in a lack of consistent sentiment assessment criteria across thousands of tobacco brands; (2) overlooking the syntactic dependencies between Chinese… More >

  • Open Access

    ARTICLE

    Genome-Wide Identification and Expression Analysis of Calmodulin-Like Proteins in Tobacco

    Mengjie Xu, Anbin Wang, Tonghong Zuo, Hecui Zhang, Zhihao Hu, Liquan Zhu*

    Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 157-179, 2025, DOI:10.32604/phyton.2025.060566 - 24 January 2025

    Abstract Calmodulin-like (CMLs) proteins are critical in calcium signaling and essential for plant growth, development, and stress responses. In many species, the CMLs families have been identified and described. However, the characterization and expression profiling of CMLs genes in tobacco is retrievable. In this study, a comprehensive whole-genome identification and analysis, and 75 NtCML genes were identified in tobacco, each containing two to four EF-hand domains. Most NtCML proteins exhibited conserved gene structures and motifs. Notably, most NtCML proteins were intron-less and distributed across 18 chromosomes. Two pairs of tandemly duplicated genes and seven pairs of segmentally More >

  • Open Access

    ARTICLE

    Elicitation of PVY Resistance by Coniothyrium aleuritis

    Mohsen Mohamed Elsharkawy1,*, Mari Sumayli2, Faisal Ay Alzahrani3

    Phyton-International Journal of Experimental Botany, Vol.93, No.12, pp. 3373-3385, 2024, DOI:10.32604/phyton.2024.058875 - 31 December 2024

    Abstract Endophytes associated with plants are recognized as bio-reservoirs of natural products and denote a significant symbiotic interaction in nature. Endophytes penetrate the plant’s interior tissues without showing any indications of disease or obvious alterations. In this study, the potential of a novel and new isolated plant growth-stimulating fungus, Coniothyrium aleuritis, was evaluated against PVY (the pathogen potato virus Y) on potato plants. Many parameters, including disease severity, PVY titer, enzymatic profiling, defense-related biochemical marker (carotenoid), phenolic compounds, proline content, as well as growth and yield parameters, have been investigated to clarify the role of C. aleuritis isolate… More > Graphic Abstract

    Elicitation of PVY Resistance by <i>Coniothyrium aleuritis</i>

  • Open Access

    ARTICLE

    The Maize WRKY Transcription Factor ZmWRKY25 Respond Drought Stress in Transgenic Tobacco

    Jianbo Fei1,2,4, Zhibo Liu1, Piwu Wang1,3, Jing Qu2, Siyan Liu1, Shuyan Guan1,3,*, Yiyong Ma1,3

    Phyton-International Journal of Experimental Botany, Vol.93, No.12, pp. 3617-3635, 2024, DOI:10.32604/phyton.2024.052704 - 31 December 2024

    Abstract The WRKY transcription factors play important roles in various biological processes such as plant development, defense regulation, and stress response. Despite this, there is limited information available on drought-related WRKY genes in maize. Through RNA sequencing (RNA-seq) analysis, a WRKY transcription factor called ZmWRKY25 was identified in this study. ZmWRKY25 belongs to Group II and is localized in the nucleus. Protein interaction analysis revealed that ZmWRKY25 interacts with several proteins involved in the abscisic acid (ABA) signal pathway. Expression of ZmWRKY25 was found to be up-regulated in response to drought, salt, and ABA treatments in tobacco plants. More >

  • Open Access

    ARTICLE

    Slag Characteristics of Biomass Pellet Fuels with Different Additives to Tobacco-Stalk for Tobacco Curing Heating

    Jianan Wang1,*, Yikuan Fan2, Weidong Duan3, Zhaopeng Song1

    Energy Engineering, Vol.121, No.11, pp. 3401-3415, 2024, DOI:10.32604/ee.2024.055158 - 21 October 2024

    Abstract Since pure tobacco stalk (TS) biomass pellet fuels tend to slag, five anti-slagging agents were added to the crushed TS to obtain a pellet fuel that could be used in biomass burners to provide heat for tobacco curing. The obtained results revealed that the main component of TS pellet fuel was K2Si2O5. During fuel combustion process, additives generated higher melting point silicate compounds by Al–K, Ca–K, and Ca–K elemental structures to replace single K elemental structure of TS, enhancing the anti-slagging efficiency of the pellet fuel from 21.63% to 78.29% and promoting the precipitation of K, More >

  • Open Access

    REVIEW

    Plant Chemical Defenses against Insect Herbivores—Using the Wild Tobacco as a Model

    Guangwei Sun1,2,#, Xuanhao Zhang3,#, Yi Liu3, Liguang Chai2, Daisong Liu2, Zhenguo Chen1,*, Shiyou Lü3,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.4, pp. 641-659, 2024, DOI:10.32604/phyton.2024.049285 - 29 April 2024

    Abstract The Nicotiana genus, commonly known as tobacco, holds significant importance as a crucial economic crop. Confronted with an abundance of herbivorous insects that pose a substantial threat to yield, tobacco has developed a diverse and sophisticated array of mechanisms, establishing itself as a model of plant ecological defense. This review provides a concise overview of the current understanding of tobacco’s defense strategies against herbivores. Direct defenses, exemplified by its well-known tactic of secreting the alkaloid nicotine, serve as a potent toxin against a broad spectrum of herbivorous pests. Moreover, in response to herbivore attacks, tobacco enhances… More >

  • Open Access

    ARTICLE

    Comparison of Combustion Characteristics of Tars Produced with Tobacco Stem Biomass Gasification

    Bo Chen1, Mingjun Wang2, Bo Liu3,*, Chunping Lu4, Guohai Jia1, Yong Chao5, Chao Zhong1

    Journal of Renewable Materials, Vol.12, No.1, pp. 119-129, 2024, DOI:10.32604/jrm.2023.031521 - 23 January 2024

    Abstract In order to study the combustion characteristics of tar in biomass gasifier inner wall and gasification gas, “tobacco stem semi-tar inside furnace”, “tobacco stem tar inside furnace” and “tobacco stem tar out-of-furnace” were subjected to thermogravimetric experiments, and the combustion characteristics and kinetic characteristics were analyzed. The result shows that “tobacco stem semi-tar inside furnace” has the highest value and “tobacco stem tar out-of-furnace” is has the lowest value on ignition characteristics, combustion characteristics and combustible stability; “tobacco stem semi-tar inside furnace” has the lowest value and “tobacco stem tar outside furnace” has the highest More > Graphic Abstract

    Comparison of Combustion Characteristics of Tars Produced with Tobacco Stem Biomass Gasification

  • Open Access

    ARTICLE

    Ectopic Overexpression of EuCHIT30.7 Improves Nicotiana tabacum Resistance to Powdery Mildew

    Lanjun Li1, Degang Zhao1,2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3043-3061, 2023, DOI:10.32604/phyton.2023.031175 - 24 October 2023

    Abstract Various strains of powdery mildew (PM), a notorious plant fungal disease, are prevalent and pose a significant threat to plant health. To control PM, transgenic technology can be used to cultivate more resistant plant varieties. In the present study, we utilized the rapid amplification of cDNA ends (RACE) technique to clone the full-length cDNA sequence of the EuCHIT30.7 gene to explore plant genes with disease resistance functions. Bioinformatics analysis revealed that this gene belongs to the GH18 family and is classified as a class III chitinase. The EuCHIT30.7 gene is expressed throughout the Eucommia ulmoides plant, with… More >

  • Open Access

    ARTICLE

    Transcriptome and Metabolome Revealed the Mechanism of NtBRL3 Overexpression Tobacco (Nicotiana tabacum L. K326) in Response to Drought Stress

    Jing Yang, Tianxiunan Pu, Ke Wan, Linqi Wang, Yuanshuai Shi, Xu Luo, Jie Tan, Dongmei Wang, Yang Liu*

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2555-2576, 2023, DOI:10.32604/phyton.2023.030301 - 28 July 2023

    Abstract Drought has severely affected the yield and quality of commercial crops. The BRI1 family plays an important role in plant response to drought stress, and BRL3 gene plays an important role in the study of drought in Arabidopsis thaliana. In this study, NtBRL3 was constructed as a vector and genetically transformed to obtain ‘N. Tobacco K326’ overexpression of NtBRL3. The enzyme activities of transgenic tobacco and wild-type tobacco were measured and transcriptome and metabolome analyses were performed. The results showed that the antioxidant enzymes of transgenic tobacco were more active under drought conditions, and 85 significantly differentially metabolites and More >

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