FANGLIAN HE¹, WEIQING DONG^1,*, SHAOLONG WEI^2,*, ZUYANG QIU³, JINGLI HUANG⁴, HUIPING JIANG¹, SHIYU HUANG¹, LILI LIU³

BIOCELL, Vol.45, No.3, pp. 785-796, 2021, DOI:10.32604/biocell.2021.014418

Abstract Taro (Colocasia esculenta (L.) Schott) is an important crop in Africa, Southeast Asia, and subtropics and is used as a food and medicine. The purple color pigmentation is an appealing character in taro. We sampled taro corms of the cultivar ‘Lipu Taro’ at four developmental stages, including LPYS1 (without purple pigment, 50 days of development (DOD)), LPYS2 (very few purple pigments, 75 DOD), LPYS3 (moderate purple pigments, 115 DOD) and LPYS4 (high purple pigments, 205 DOD). The purpose of our study was to identify the key genes underpinning the purple pigmentation in taro based on RNA-sequencing. Through RNA-Seq, 6453 differentially… More >

YANFEI LI¹, LULU DAI², KE CAI², YINGKUI SONG², XIQING LIU^3,*

BIOCELL, Vol.45, No.3, pp. 685-694, 2021, DOI:10.32604/biocell.2021.013612

Abstract RPA3 (Replication Protein A3) (14 kD) is a part of the canonical heterotrimeric replication protein A complex (RPA/RP-A). This study aimed to explore the functional role of RPA3 and the mechanisms of its dysregulation in breast cancer. Data from the Cancer Genome Atlas (TCGA)-breast cancer patients and GSE75688 were utilized for gene expression and survival analysis. Breast cancer cell lines MDA-MB-231 and SK-BR-3 were used for in-vitro cell studies. Clonogenic assay and immunofluorescent staining of γ-H2AX were performed to examine radiation-induced cytotoxicity. Systemic correlation analysis was performed to identify potential transcription factors (TFs) regulating RPA3 expression. ChIP-qPCR and dual-luciferase assay… More >

Víctor A. Sánchez-Camargo^1,2, Samantha Romero-Rodríguez¹, Jorge M. Vázquez-Ramos^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 307-330, 2021, DOI:10.32604/phyton.2021.014967

Abstract The E2F/DP pathway is a widely conserved regulatory mechanism in pluricellular organisms. The family of E2F and DP transcription factors was originally described having a role in the transition from the G1 to the S phase of the cell cycle. However, the discovery of hundreds of possible gene targets and their involvement in many other biochemical processes, soon showed that they participated in cell development and differentiation, chromatin remodeling, DNA repair and others. The E2F/DP transcription factors can act as either activators or repressors of transcription depending on their association to other regulatory proteins, particularly the retinoblastoma protein, or even… More >

Haoliang Zhang^1,*, Xiaowei Xing², Yang Liu¹, Shuangli Li¹, Weiyuan Li³

Oncologie, Vol.22, No.2, pp. 107-116, 2020, DOI:10.32604/oncologie.2020.012494

Abstract Lung cancer is the leading cause of death in cancer patients. Based on a modular and comprehensive analysis method, it is intended to identify their common pathogenesis. We downloaded data and analyzed differences in lung adenocarcinoma samples, lung squamous cell carcinoma samples, and normal samples. Co-expression analysis, enrichment analysis, and hypergeometric testing were used to predict transcription factors, ncRNAs, and retrospective target genes. We get 4596 differentially expressed genes in common differences in high multiples and clustered into 14 modules dysfunction. The 14 genes (including DOK2, COL5A1, and TSPAN8) have the highest connectivity in the dysfunction module and are identified… More >

Danni He, Guoli Deng, Songpei Ying, Wenjuan Yang, Jiali Wei, Ping Li^*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 633-643, 2020, DOI:10.32604/phyton.2020.010498

Abstract Primary seed dormancy is an adaptive strategy that prevents germination for viable seeds in harsh environment, ensuring seeds germination under favorable condition. Accurately inducing seeds germination in a controllable manner is important for crop production. Thus searching the chemicals that efficiently breaks seed dormancy is valuable. DOG1 protein abundance in the freshly harvested seed is high, and its level is correlated to seed dormancy intensity, thus DOG1 is regarded as the timer to evaluate the seed dormancy degree. In this study, we found the carbon monoxide (CO) donor treatment, the transgenic line with high CO content, showed lower seed dormancy,… More >

Tamilselvan SUBRAMANI^1,2, Suresh RAO², Kamatchiammal SENTHILKUMAR³, Soundararajan PERIASAMY⁴, Noorjahan Banu ALITHEEN¹

BIOCELL, Vol.37, No.3, pp. 71-76, 2013, DOI:10.32604/biocell.2013.37.071

Abstract The present study demonstrates that the expression of c-Jun and c-Fos are elevated in gingival fibroblast cells treated with angiotensin II and cyclosporine. The healthy human gingival tissues were collected and gingival fibroblasts were isolated and cultured. We used RT-PCR and Western blot analysis to identify the expression of c-Jun and c-Fos in cyclosporine and angiotensin II treated human gingival fibroblast cells. We found that angiotensin II in combination with cyclosporine induces c-Jun and c-Fos expressions significantly; however, the angiotensin II antagonist losartan inhibits the expression of c-Jun and c-Fos (p < 0.01). The data suggest that angiotensin II in… More >

CUIXIA CHEN^1*, LINGLING CUI², XIN SHANG², XIANLU ZENG²

BIOCELL, Vol.34, No.2, pp. 57-64, 2010, DOI:10.32604/biocell.2010.34.057

Abstract L-selectin is a member of the selectin family that play an important role both in mediating the initial capture and subsequent rolling of leukocytes along the endothelial cells. Furthermore, L-selectin can function as a signal molecule. In our previous studies, we reported that L-selectin ligation could regulate CSF-1 (colony-stimulating factor-1) gene transcription, in which AP-1 acts as a crucial transcriptional factor. Here we investigated the function of the NFAT in the CSF-1 gene transcriptional events. We found that overexpression of WT NFAT induce CSF-1 gene transcription greatly in the activated Jurkat cells. Furthermore, we found that NFAT can be recruited… More >

Chan RL

Phyton-International Journal of Experimental Botany, Vol.78, pp. 5-10, 2009, DOI:10.32604/phyton.2009.78.005

Abstract Transcription factors (TFs) are proteins able to specifically recognize DNA sequences in the regulatory regions of their target genes. They bind these specific sequences, an event that leads to the activation or repression of whole signal transduction pathways. In plants about 1500 TFs were informatically identified; identification was mainly based in the presence of DNA-binding domains in the translated sequences. They were classified in families and subfamilies according to several features, including the conservation of the DNA binding domain, the genes structures and the functions they exert. Among transcription factors, several seem to be potential powerful biotechnological tools to improve… More >

HAIFENG WANG^1,2, KAI WANG¹, JUAN GUO¹, TIEQIAO WEN²

BIOCELL, Vol.43, No.2, pp. 59-64, 2019, DOI:10.32604/biocell.2019.05731

Abstract Histone deacetylation is a key modulator involved in cell proliferation, apoptosis, and mRNA transcription. However, the effects of histone deacetylation on C17.2 neural stem cells (NSCs) remain unclear. Here, the histone deacetylase inhibitors nicotinamide and trichostatin A (TSA) were used to determine the role of histone deacetylation on gene transcription in NSCs. The results showed that the mRNA expression of p53, Sox1, Sox2, and Bax were significantly higher in E14.5 NSCs than in C17.2 NSCs. Nestin, a marker gene of neuronal differentiation, did not differ significantly between E14.5 NSCs and C17.2 NSCs. The transcription levels of p53 and Nestin were… More >