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  • Open Access

    ARTICLE

    Transcriptome Analysis of Inflorescence Development at the Five-Leaf Stage in Castor (Ricinus communis L.)

    Yong Zhao1,#, Yaxuan Jiang3,#, Li Wen1, Rui Luo2, Guorui Li2, Jianjun Di2, Mingda Yin2, Zhiyan Wang2, Fenglan Huang2,4,5,6,7,*, Fanjuan Meng3,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.4, pp. 713-723, 2024, DOI:10.32604/phyton.2024.047657

    Abstract The yield of castor is influenced by the type of inflorescence and the proportion of female flowers. However, there are few studies on the genetic mechanism involved in the development and differentiation of castor inflorescences. In this study, we performed transcriptomic analyses of three different phenotypes of inflorescences at the five-leaf stage. In comparison to the MI (complete pistil without willow leaves), 290 and 89 differentially expressed genes (DEGs) were found in the SFI (complete pistil with willow leaves) and the BI (monoecious inflorescence), respectively. Among the DEGs, 104 and 88 were upregulated in the SFI and BI, respectively, compared… More >

  • Open Access

    ARTICLE

    Physiological and Transcriptome Analysis Illuminates the Molecular Mechanisms of the Drought Resistance Improved by Alginate Oligosaccharides in Triticum aestivum L.

    Yunhong Zhang1,2,*, Yonghui Yang1,2, Jiawei Mao1,2

    Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 185-212, 2024, DOI:10.32604/phyton.2023.046811

    Abstract Alginate oligosaccharides (AOS) enhance drought resistance in wheat (Triticum aestivum L.), but the definite mechanisms remain largely unknown. The physiological and transcriptome responses of wheat seedlings treated with AOS were analyzed under drought stress simulated with polyethylene glycol-6000. The results showed that AOS promoted the growth of wheat seedlings and reduced oxidative damage by improving peroxidase and superoxide dismutase activities under drought stress. A total of 10,064 and 15,208 differentially expressed unigenes (DEGs) obtained from the AOS treatment and control samples at 24 and 72 h after dehydration, respectively, were mainly enriched in the biosynthesis of secondary metabolites (phenylpropanoid biosynthesis,… More >

  • Open Access

    EDITORIAL

    Editorial: Transcriptome analysis in tumor microenvironment and tumor heterogeneity

    JINHUI LIU1,*, JIAHENG XIE2,*, PEIXIN DONG3

    Oncology Research, Vol.32, No.1, pp. 99-100, 2024, DOI:10.32604/or.2023.045719

    Abstract This article has no abstract. More >

  • Open Access

    ARTICLE

    Transcriptome Analysis of Molecular Mechanisms Underlying Phenotypic Variation in Phaseolus vulgaris Mutant ‘nts’

    Limin Yin#, Chang Liu#, Zicong Liang, Dajun Liu, Guojun Feng, Zhishan Yan*, Xiaoxu Yang*

    Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 2981-2998, 2023, DOI:10.32604/phyton.2023.043151

    Abstract The phenotype of a common bean plant is often closely related to its yield, and the yield of plants with reduced height or poor stem development during growth is low. Mutants serve as an essential gene resource for common bean breeding genetic research. Although model plants and crops are studied to comprehend the molecular mechanisms and genetic basis of plant phenotypes, the molecular mechanism of phenotypic variation in common beans remains underexplored. We here used the mutant ‘nts’ as material for transcriptome sequencing analysis. This mutant was obtained through 60Co-γ irradiation from the common bean variety ‘A18’. Differentially expressed genes… More >

  • Open Access

    ARTICLE

    Comparative transcriptome analysis provides insights into the molecular mechanism of the anti-nematode role of Arachis hypogaea (Fabales: Fabaceae) against Meloidogyne incognita (Tylenchida: Heteroderidae)

    XUEJIN YANG1,2,#, YUANYUAN ZHOU1,#, XINYI PENG1, XIAOHONG FU1, JIANQING MA1, JIANFENG LIU1,*, DANDAN CAO1,2,*

    BIOCELL, Vol.47, No.9, pp. 2101-2113, 2023, DOI:10.32604/biocell.2023.029581

    Abstract Background: Plant root-knot nematode (RKN) disease is a serious threat to agricultural production across the world. Meloidogyne incognita is the most prominent pathogen to the vegetables and cash crops cultivated. Arachis hypogaea can effectively inhibit M. incognita, but the underlying defense mechanism is still unclear. Methods: In our study, the chemotaxis and infestation of the second-stage juveniles (J2s) of M. incognita to A. hypogaea root tips were observed by the Pluronic F-127 system and stained with sodium hypochlorite acid fuchsin, respectively. The transcriptome data of A. hypogaea roots with non-infected or infected by J2s were analyzed. Results: The J2s could… More >

  • Open Access

    ARTICLE

    Comparative Transcriptome Analysis of Seed Germination of a Cotton Variety with High Tolerance to Low Temperature

    Genhai Hu1,*, Maoni Chao1, Xiuren Zhou2, Yuanzhi Fu2

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2535-2554, 2023, DOI:10.32604/phyton.2023.030163

    Abstract Gossypium hirsutum L. is an important cash crop native to the subtropics and is widely cultivated around the world. Low temperature is an important stress that seriously affects seed germination and emergence during planting. In this study, transcriptomic profiles of low-temperature- and normal-temperature-germinated seeds of Xinluzao 25, a variety with low-temperature tolerance and high germination rates, were analyzed and compared. The following results were obtained. (1) A total of 81.06 Gb of clean data were obtained after transcriptome sequencing and assembly, and 76,931 non-redundant Unigene sequences were obtained after data consolidation and concatenation; of these, 69,883 Unigene sequences were annotated.… More >

  • Open Access

    ARTICLE

    Transcriptome analysis reveals tumor antigen and immune subtypes of melanoma

    JIAHENG XIE1,#, MENGMENG OU1,#, PAN YU1,#, DAN WU2,#, QIKAI TANG3, YUAN CAO4, JING HANG5, LU YIN1, TINGHONG XIANG1, MING WANG1,*, JINGPING SHI1,*

    Oncology Research, Vol.31, No.3, pp. 389-403, 2023, DOI:10.32604/or.2023.029274

    Abstract Purpose: To screen potential tumor antigens for melanoma vaccine development and identify different immune subtypes. Methods: Transcriptional data (HTSEQ-FPKM) and clinical information of a 472 Melanoma cohort GDC TCGA Melanoma (SKCM) were downloaded from the UCSC XENA website (). Subsequently, transcriptome data and clinical information of 210 melanoma cohort GSE65904 were downloaded from Gene Expression Omnibus (GEO), a large global public database. All the transcriptome expression data matrices were log2 transformed for subsequent analysis. GEPIA, TIMER, and IMMPORT databases are also used for analysis. Cell function experiments were performed to validate the role of the IDO1 gene in melanoma cell… More >

  • Open Access

    ARTICLE

    Transcriptome Analysis of Auxin Drives Cone Size Regulation in Fokienia hodginsii

    Shunde Su1,2,#, Huan Chang3,#, Xiangyang Kang1,*, Renhua Zheng2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1713-1723, 2023, DOI:10.32604/phyton.2023.027586

    Abstract As the reproductive organ of the endangered species Fokienia hodginsii, the size of the cones is a constraint on the reproductive renewal of the population. In this study, the molecular basis of the influence of cone size on F. hodginsii was elucidated by comparing the phenotype, biochemistry, and transcriptome of two cultivars of F. hodginsii (‘FJ431’ and ‘FJ415’). The two cultivars differed significantly in cone size, with FJ431 having a significantly larger cone size and weight than FJ415, 1.32 and 1.90 times that of FJ415, respectively. RNA-Seq analysis of both cultivars retrieved 75,940 genes whose approximate functions were classified as… More >

  • Open Access

    ARTICLE

    Comparative Non Participating Transcriptome Analysis Response to Low Phosphorus by CmPht1;2 in Chrysanthemum

    Chen Liu1,2 , Xiaowei Tang1,2, Xiaohui Qu1,2 , Fadi Chen1, Jiafu Jiang1 and Sumei Chen1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1391-1404, 2023, DOI:10.32604/phyton.2023.027231

    Abstract Chrysanthemum morifolium Ramat is one of the four major cut flowers in the world. Pht1 family is focus on the uptake and transport of phosphate in plants. In our previous studies, CmPht1;2 overexpression line (Oe1) had higher phosphate contents both in roots and shoots, and its root development was significantly enhanced than wild type (WT) at low phosphorus conditions in chrysanthemum. Metabolomics analysis showed that several metabolites had a change in pyruvate metabolism and tricarboxylic acid (TCA) cycle pathway. To explore the gene difference expression and the change of metabolic pathway between CmPht1;2-Oe1 and WT, we conducted the transcriptome analysis.… More >

  • Open Access

    ARTICLE

    Transcriptome analysis reveals potential genes associated with plant height in rice

    CAN CHEN#, RUI FENG#, ZONGQIONG ZHANG#, XIUZHONG XIA, BAOXUAN NONG, YU ZENG, HUI GUO, XINGHAI YANG*, DANTING LI*

    BIOCELL, Vol.47, No.2, pp. 409-421, 2023, DOI:10.32604/biocell.2023.023543

    Abstract Plant height (PH) is a complex trait regulated by the environment and multiple genes. PH directly affects crop yield, harvest index, and lodging resistance. From plant dwarf mutants, many genes related to PH have been identified and described. Nonetheless, the molecular mechanism of height regulation in high-culm rice mutants has not been well studied. By using transcriptome and weighted gene co-expression network analysis (WGCNA), we identified the differentially expressed genes (DEGs) between high-culm rice mutants (MUT) and wild-type (WT) and explored the key pathways and potential candidate genes involved in PH regulation. Transcriptome analysis identified a total of 2,184 DEGs,… More >

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