Nada Zaari Jabri¹, Mohamed Ait-El-Mokhtar^1,*, Fadoua Mekkaoui¹, Najwa Rabah¹, Ilham Amghar¹, Ghizlane Diria², Abdelaziz Hmyene¹

Phyton-International Journal of Experimental Botany, Vol.94, No.11, pp. 3687-3701, 2025, DOI:10.32604/phyton.2025.071634 - 01 December 2025

Abstract Cadmium (Cd) contamination is a major environmental stressor that adversely affects crop germination and early development. This study assessed the impact of increasing Cd concentrations (0.125 to 1 g/L) on seed germination and early seedling growth in three bread wheat (Triticum aestivum L.) cultivars: Achtar, Lina, and Snina. The results revealed a clear dose-dependent inhibitory effect of Cd. Germination percentage (GP) significantly declined with increasing Cd levels, while mean germination time was progressively delayed, particularly at higher concentrations. Vigor index (VI) also showed significant reductions, reflecting compromised seedling establishment. Morphological traits, especially shoot and root lengths,… More >

Mutong Li^1,#, Xiaolian Yang^1,#, Zhishun Yu¹, Tingting Yuan¹, Wenjie Wang¹, Suqin Zhang^1,2,*, Guangdong Geng^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3175-3188, 2025, DOI:10.32604/phyton.2025.070731 - 29 October 2025

Abstract Wheat yield mainly depends on leaf photosynthesis and grain carbohydrate accumulation. The aux/indole-3-acetic acid 13-like (Aux/IAA13L) gene was successfully cloned from Tritipyrum ‘Y1805’ and transformed into common wheat. A bioinformatics analysis showed that the TtAux/IAA13L protein, encoding 232 amino acids, was hydrophilic and unstable. TtAux/IAA13L and Tel5E01G609500 were grouped together in a phylogenetic tree. The TtAux/IAA13L expression levels in the overexpression lines were higher than in the wild-type (WT) plants at five developmental stages: tillering, elongation, heading, flowering, and grain-filling. The expression levels in the overexpression lines first increased, peaked at the flowering stage, and then decreased.… More >

Jing Zhang^1,#, Huifen Qiao^1,#, Shenglong Wang^1,#, Jiawei Yuan¹, Qingsong Ba¹, Gensheng Zhang^1,2,*, Guiping Li^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2701-2718, 2025, DOI:10.32604/phyton.2025.070017 - 30 September 2025

Abstract Wheat stripe rust, a devastating disease caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), poses a significant threat to global wheat production. Growing resistant cultivars is a crucial strategy for wheat stripe rust management. However, the underlying molecular mechanisms of wheat resistance to Pst remain incompletely understood. To unravel these mechanisms, we employed high-throughput RNA sequencing (RNA-Seq) to analyze the transcriptome of the resistant wheat cultivar Mianmai 46 (MM46) at different time points (24, 48, and 96 h) post-inoculation with the Pst race CYR33. The analysis revealed that Pst infection significantly altered the expression of genes… More >

Sumeyra Ucar¹, Muhammed Aldaif ², Esra Yaprak¹, Esma Yigider ², Murat Aydin^2,*, Emre Ilhan¹, Abdulkadir Ciltas², Ertan Yildirim³

Phyton-International Journal of Experimental Botany, Vol.94, No.8, pp. 2461-2477, 2025, DOI:10.32604/phyton.2025.068383 - 29 August 2025

Abstract Wheat is a crucial crop for global food security, and effective in vitro plant regeneration techniques are considered a precondition for genetic engineering in wheat breeding programs. A practical approach for in vitro regeneration of the Kırik bread wheat cultivar via somatic embryogenesis was investigated using endosperm-supported mature embryos. Callus cultures were initiated from mature embryos supported by endosperm, cultured on phytagel-based Murashige and Skoog (MS) basal medium containing dicamba (12 mg/L) and indole-3-acetic acid (IAA) (0.5 mg/L) under dark conditions. This research was designed to examine the impact of putrescine (Put) (0.0 and 1.0 mM) on… More >

E. M. B. M. Karunathilake^1,#, Thanh Tuan Thai^1,2,3,#, Sheikh Mansoor¹, Anh Tuan Le^3,4, Faheem Shehzad Baloch^1,5, Yong Suk Chung^1,*, Dong-Wook Kim^6,*

Phyton-International Journal of Experimental Botany, Vol.94, No.7, pp. 2215-2228, 2025, DOI:10.32604/phyton.2025.067439 - 31 July 2025

Abstract Accurate and timely estimation of above-ground biomass is crucial for understanding crop growth dynamics, optimizing agricultural input management, and assessing productivity in sustainable farming practices. However, conventional biomass assessments are destructive and resource-intensive. In contrast, remote sensing techniques, particularly those utilizing low-altitude unmanned aerial vehicles, provide a non-destructive approach to collect imagery data on plant canopy features, including spectral reflectance and structural details at any stage of the crop life cycle. This study explores the potential visible-light-derived vegetative indices to improve biomass prediction during the flowering period of buckwheat (Fagopyrum tataricum). Red, green, and blue (RGB)… More >

Shivani Sood¹, Harjeet Singh^2,*, Surbhi Bhatia Khan^3,4,5,*, Ahlam Almusharraf⁶

CMC-Computers, Materials & Continua, Vol.84, No.2, pp. 2751-2787, 2025, DOI:10.32604/cmc.2025.060185 - 03 July 2025

Abstract Wheat fungal infections pose a danger to the grain quality and crop productivity. Thus, prompt and precise diagnosis is essential for efficient crop management. This study used the WFD2020 image dataset, which is available to everyone, to look into how deep learning models could be used to find powdery mildew, leaf rust, and yellow rust, which are three common fungal diseases in Punjab, India. We changed a few hyperparameters to test TensorFlow-based models, such as SSD and Faster R-CNN with ResNet50, ResNet101, and ResNet152 as backbones. Faster R-CNN with ResNet50 achieved a mean average precision More >

Andris Berzins^1,2, Ramunas Tupciauskas^1,*, Gunars Pavlovics¹, Martins Andzs¹

Journal of Renewable Materials, Vol.13, No.6, pp. 1189-1207, 2025, DOI:10.32604/jrm.2025.02024-0067 - 23 June 2025

Abstract This study examines the development of loose-fill thermal insulation materials derived from annual plant residues, such as wheat straw, water reeds, and corn stalks, processed using the chemimechanical pulping (CMP) technique. The chopped plants were soda-cooked for 30 min, varying NaOH concentration (2%–8% on a dry basis of biomass), and mechanically refined using different disc types. The CMP process enhances the homogeneity and stability of defibrated material, yielding improved insulation properties compared to untreated chopped raw materials. Chemical analysis revealed that CMP increases cellulose content and reduces lignin levels, enhancing water retention and vapor diffusion… More > Graphic Abstract

Hussam Qushtom, Ahmad Hasasneh^*, Sari Masri

CMC-Computers, Materials & Continua, Vol.84, No.1, pp. 1379-1395, 2025, DOI:10.32604/cmc.2025.061995 - 09 June 2025

Abstract This study presents an enhanced convolutional neural network (CNN) model integrated with Explainable Artificial Intelligence (XAI) techniques for accurate prediction and interpretation of wheat crop diseases. The aim is to streamline the detection process while offering transparent insights into the model’s decision-making to support effective disease management. To evaluate the model, a dataset was collected from wheat fields in Kotli, Azad Kashmir, Pakistan, and tested across multiple data splits. The proposed model demonstrates improved stability, faster convergence, and higher classification accuracy. The results show significant improvements in prediction accuracy and stability compared to prior works,… More >

S. Y. Labonno, M. S. Raihan, M. Mohi-Ud-Din, A. K. M. Aminul Islam^*

Phyton-International Journal of Experimental Botany, Vol.94, No.5, pp. 1545-1563, 2025, DOI:10.32604/phyton.2025.063916 - 29 May 2025

Abstract Morpho-physiological evaluation of a crop’s genetic resources is necessary to find possible genotypes to include in breeding initiatives. The objective of this study was to identify heat-tolerant wheat genotypes among36 mutant lines using morpho-physiological indices. Seedlings of mutant lines and check varieties were grown under both normal (control) and heat-stress conditions in growth chambers. Data were recorded on root-shoot parameters (length, fresh weight, dry weight, and ratio), relative water content (RWC), stability of cell membrane, pigment content, and chlorophyll fluorescence. Two-way analysis of variance showed significant (p < 0.01, p < 0.001) variation among15 morpho-physiological features… More >

Mudasser Mehmood^1,*, Zoahaib Aslam Khan¹, Adil Mehmood², Madiha Zaynab³, Muhammad Atiq ur Rahman⁴, Mohammad Khalid Al-Sadoon⁵, M. Harshini⁶, Ling Shing Wong⁷

Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1111-1135, 2025, DOI:10.32604/phyton.2025.059812 - 30 April 2025

Abstract Wheat (Triticum aestivum L.) is a staple crop critical for global food security, yet its productivity is significantly affected by abiotic stresses such as drought, salinity, and waterlogging, which are exacerbated by climate change. This study evaluated the effects of these stresses on vegetative growth, physiological responses, and yield. Field experiments were conducted using a Randomized Complete Block Design (RCBD) at the Mona Reclamation Experimental Project (MREP), WAPDA, Bhalwal, Sargodha, Punjab Pakistan. Stress treatments included three levels of drought (25%, 50%, and 75% field capacity), salinity (4, 8, and 12 dS/m), and waterlogging (24, 48, and… More >