Hua Zhang^#, Zhongmin Yang^#, Xilong You, Youqiang Heng, Yan Wang^*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 389-402, 2021, DOI:10.32604/phyton.2021.014156

Abstract Potassium (K⁺) is a necessary nutrient for plant growth and crop production. The K⁺ transporter plays crucial roles in the absorption and transport of K⁺ in plants. Most K⁺ transporters in Arabidopsis have been reported, but AtKUP12, which is a member of the KT/KUP/HAK family, has not yet been the subject of relevant in-depth research. In the present study, we demonstrated that AtKUP12 plays a crucial role in K⁺ uptake in Arabidopsis under 100 μM lowK⁺ and 125 mM salt stress conditions. AtKUP12 transcripts were induced by K⁺ deficiency and salt stress. We analyzed the K⁺ uptake of AtKUP12 using… More >

Mingda Yang¹, Shouchen Ma², Fujian Mei¹, Li Wei¹, Tongchao Wang^1,*, Xiaokang Guan^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 417-435, 2021, DOI:10.32604/phyton.2021.013175

Abstract Knowledge of the interactive effects of water and nitrogen (N) on physio-chemical traits of maize (Zea mays L.) helps to optimize water and N management and improve productivity. A split-plot experiment was conducted with three soil water conditions (severe drought, moderate drought, and fully water supply referring to 45%–55%, 65%–75%, and 85%–95% field capacity, respectively) and four N application rates (N₀, N₁₅₀, N₂₄₀, and N₃₃₀ referring to 0, 150, 240, 330 kg N ha^–1 respectively) under drip fertigation in 2014 and 2015 in the Huang-Huai-Hai Plain of China. The results indicated that drought stress inhibited physiological activity of plants (leaf… More >

Jiaji Zhang¹, Xiaofei Long¹, Yuhao Weng¹, Tielong Cheng², Jisen Shi¹, Jinhui Chen^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 571-582, 2021, DOI:10.32604/phyton.2021.014191

Abstract Cinnamomum japonicum Sieb. is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value. In this study, we successfully developed a large-scale micropropagation protocol for C. japonicum for the first time. Sterilized shoots were excised and used as explants for shoot induction on several basal media, supplemented with different concentrations of plant growth regulators (PGRs), such as Thidiazuron (TDZ), N⁶ -Benzyladenine (6-benzylaminopurine) (BA), α-naphthaleneacetic acid (NAA) and Gibberellic acid (GA₃). After comparison, the most efficient medium for shoot regeneration was 1/2 Murashige and Skoog (MS) medium containing 0.5 mg L^–1 BA, 0.05 mg L^–1 NAA and… More >

Anna Stepanova^1,*, Maria Malunova¹, Svetlana Salamaikina¹, Renat Selimov², Aleksandra Solov’eva¹

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 543-552, 2021, DOI:10.32604/phyton.2021.013223

Abstract Rhodiola quadrifida is a rare mountain medicinal plant whose root extracts are used in traditional Chinese medicine as a hemostatic, antitussive, and tonic in the treatment of gynecological diseases. The aim of the study was to obtain R. quadrifida cultures at different degrees of differentiation in vitro and compare their growth characteristics and the content of salidroside and rosavin. Hairy roots were obtained by incubating cotyledons and hypocotyls in a suspension of Agrobacterium rhizogenes strain A4. The presence of the rolB and rolC genes was proven by polymerase chain reaction. The obtained roots were cultivated in Murashige-Skoog medium (MS). Calluses… More >

Monika C. Dayarathne¹, Rashika S. Brahmanage², Kevin D. Hyde^2,3, Yong Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 677-686, 2021, DOI:10.32604/phyton.2021.012897

Abstract A fungus similar to the monotypic genus Natarajania, isolated from dead wood and collected in Thailand, is reported. Analysis of partial ribosomal LSU and a protein coding gene (RPB2) demonstrated that the new isolate belonged to Stilbosporaceae, Diaporthales and genetically different from N. indica. It is unique in producing synnematous conidiophores, smooth-walled conidiogenous cells and a flared collarette but lacks an elongated collar-canal which is distinct in the type species. Therefore, sequence data and morphological traits are used to introduce the new species, Natarajania thailandica. More >

Dabing Xiang¹, Wei Wei¹, Yan Wan¹, Xiaoyong Wu¹, Xueling Ye¹, Lianxin Peng¹, Linyun Zhong², Qi Wu¹, Liang Zou¹, Gang Zhao^1,*, Jianglin Zhao^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 461-473, 2021, DOI:10.32604/phyton.2021.011724

Abstract Drought can limit the growth and reduce the yield of crops, but the safe and effective bio-approach to improve the drought resistance of crops is very little. We conducted an experiment in which we monitored the effects of polysaccharide from the endophyte Bionectria sp. Fat6 on the growth of Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) seedlings under control and drought-stressed conditions by determining gas exchange, photosynthesis parameters, photosynthetic pigment contents, and metabolite accumulation. Results indicated that the polysaccharide from endophyte stimulated plant growth and increased the aboveground biomass, root mass, and root/shoot ratio of Tartary buckwheat. Application of the polysaccharide… More >

Muhammad Saqib, Muhammad Akbar Anjum^*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 331-352, 2021, DOI:10.32604/phyton.2021.011685

Abstract Crop simulation models constitute the major proportion in decision support systems. A large number of crop models have been developed for potato and few for tomato and peppers. In the literature, thirty three crop models have been reported to simulate potato, nine for tomato and six for peppers. Some of these models dealt with the climate change scenario and others with the crop management practices such as sowing time, irrigation, nitrogen, and insect-pests management. The most evaluated and applied models for potato include; SUBSTOR, and LINTUL-Potato, whereas CROPGRO-tomato model is the most tested and applied for tomato. The AQUACROP is… More >

Lihan Cui¹, Lijie Yan¹, Xiaohu Zhao¹, Lin Yuan², Jing Jin³, Jingcheng Zhang^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 621-634, 2021, DOI:10.32604/phyton.2021.015511

Abstract Tea plant stresses threaten the quality of tea seriously. The technology corresponding to the fast detection and differentiation of stresses is of great significance for plant protection in tea plantation. In recent years, hyperspectral imaging technology has shown great potential in detecting and differentiating plant diseases, pests and some other stresses at the leaf level. However, the lack of studies at canopy level hampers the detection of tea plant stresses at a larger scale. In this study, based on the canopy-level hyperspectral imaging data, the methods for identifying and differentiating the three commonly occurred tea stresses (i.e., the tea leafhopper,… More >

Víctor A. Sánchez-Camargo^1,2, Samantha Romero-Rodríguez¹, Jorge M. Vázquez-Ramos^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 307-330, 2021, DOI:10.32604/phyton.2021.014967

Abstract The E2F/DP pathway is a widely conserved regulatory mechanism in pluricellular organisms. The family of E2F and DP transcription factors was originally described having a role in the transition from the G1 to the S phase of the cell cycle. However, the discovery of hundreds of possible gene targets and their involvement in many other biochemical processes, soon showed that they participated in cell development and differentiation, chromatin remodeling, DNA repair and others. The E2F/DP transcription factors can act as either activators or repressors of transcription depending on their association to other regulatory proteins, particularly the retinoblastoma protein, or even… More >

Fujia Wu¹, Chengming You², Jie Du³, Xiangjun Pei^4,*, Ningfei Lei⁴, Bo Tan², Huixing Song^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 635-650, 2021, DOI:10.32604/phyton.2021.014298

Abstract The humification degree of fresh litter directly controls the accumulation of soil humus derived from plant litter, but very little information on this process is available. Planted forests are well known to restrict soil fertility, which is often indicated by the soil humus level. In this study, fresh litter was collected during different plant phenological stages during 2016 and 2017 in a mixed plantation in Southwest China. The values of hue coefficient ΔlogK (absorbance ratio of 400 nm and 600 nm on a logarithmic scale), optical density E4/E6 (absorbance ratio of 400 nm and 600 nm) and A600/C (absorbance at… More >