Si ran Wang^#, Jia Yan^#, Bu er Ha, Yu’e Bai^*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 595-604, 2021, DOI:10.32604/phyton.2021.013693

Abstract Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration. Genetic engineering technology has been successfully used in many plant species, but it is limited in Hydrangea. Here we established an efficient regeneration system by using stem segments as explants for the first time. In our study, the plant growth regulators (PGRs) were evaluated at the different regeneration processes, including axillary shoots regeneration and root induction. We found that the optimal concentration for axillary buds’ induction was 2.0 mgL⁻¹ 6-BA and 0.5 mgL⁻¹ 1 IAA, its highest induction rate was 70%. Moreover, the highest axillary shoots proliferation coefficient… More >

Dhriti Kapoor¹, Kapani Kavani¹, Amandeep Rattan², Marco Landi^3,*, Anket Sharma^4,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 489-501, 2021, DOI:10.32604/phyton.2021.012359

Abstract Heavy metal toxicity is one of the major ecosystem concerns globally in present time and is also responsible for significant threat to agronomic crops. The current work was conducted to investigate the possible ameliorative role of proline in Coriandrum sativum L. seedlings treated with mercury (Hg). The seedlings were exposed to different concentrations of Hg (0, 0.1, 0.3 and 0.5 mM) for 20 days. The effects of pre-sowing treatment with proline were studied on C. sativum seedlings in terms of pigment (chlorophylls, carotenoids and anthocyanins), malondialdehyde (MDA), antioxidant compound (glutathione, total phenolic compounds, ascorbic acid) and osmolytes (proline, glycine betaine).… More >

Yuying Jiang^1,#, Renhua Huang^1,#, Lei Jiang¹, Ke Chen^1,*, Wenkun Zhu^2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 403-415, 2021, DOI:10.32604/phyton.2021.014376

Abstract Arbuscular mycorrhizal fungi (AMF) can improve plant tolerance to several abiotic stresses, including heavy metals, drought or salinity exposure. However, the role of AMF in alleviation of soil cadmium (Cd)-induced toxicity to plants is still largely unknown. In this study, Cd speciation in soil and subcellular distribution of Cd were used to characterize the roles of application AM fungi in the alleviation of Cd toxicity in alfalfa plants. Our results showed that the addition of Glomus mosseae in Cd contaminated soil (10 mg/Kg) significantly increased soil pH, cation exchange capacity (CEC) and organic matter in rhizosphere soil with Medicago truncatula… More >

Wei Tan¹, Mingxiu Xu¹, Siqi Xie¹, Yan Zhang¹, Shuai Wu¹, Qinyan Zou¹, Qifeng Zhao², Qingliang Li^3,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 553-570, 2021, DOI:10.32604/phyton.2021.014082

Abstract To understand the anthocyanin characteristics of wine grape varieties, the anthocyanin composition and content of 31 wine grape varieties were analyzed to explore the use of anthocyanins as chemical fingerprints to distinguish varieties. Results showed that a total of 21 anthocyanins were detected in the skins, including cyanidin, delphinidin, petunidin, peonidin and malvidin 3-monoglucosides (or 3,5-diglucosides) along with the corresponding acetyl and p-coumaroyl derivatives. The highest and lowest total amount of anthocyanins were detected in ‘Ruby Cabernet’ and ‘Muscat Rouge’, respectively. In the 21 Vitis vinifera grapes, there were 3~11 monoglucoside anthocyanins detected, however, there were 4 to 9 monoglucoside… More >

Kuipeng Li^1,2, Hua Han¹, Yunhui Xie¹, Xiaomei Sun^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 583-593, 2021, DOI:10.32604/phyton.2021.013912

Abstract Optimization of in vitro adventitious root induction contributes to the development of a large-scale production system of hybrid clone seedlings of larch (Larix spp.). We used orthogonal testing to investigate the factors that affect the rooting of hybrid larch shoots–activated carbon, vitamin B1, sucrose, glycine, glutamic acid (Glu), cysteine, pH, and induction time in the dark and indole-3-butyric acid (IBA). Variance analysis showed that the effects of sucrose concentration on rooting rate, survival rate, and average root number, pH on the average number of roots; and Glu on survival rate were all significant (p < 0.05) when compared to the… More >

Hesham F. Alharby¹, Mirza Hasanuzzaman^2,*, Hassan S. Al-Zahrani¹, Khalid Rehman Hakeem¹

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 373-388, 2021, DOI:10.32604/phyton.2021.013657

Abstract The mechanism of selenium (Se)-induced salt tolerance was studied in moderately sensitive soybean (Glycine max L.) plants. To execute this view, soybean plants were imposed with salt stress (EC 6 dS m⁻¹ ) applying NaCl. In other treatments, Se (0, 25, 50 and 75 µM Na₂SeO₄) was sprayed as co-application with that level of salt stress. Plant height, stem diameter, leaf area, SPAD value decreased noticeably under salt stress. Altered proline (Pro) level, together with decreased leaf relative water content (RWC) was observed in salt-affected plants. Salt stress resulted in brutal oxidative damage and increased the content of H₂O₂, MDA… More >

Abdul Awal Chowdhury Masud¹, Md. Fazlul Karim¹, M. H. M. Borhannuddin Bhuyan², Jubayer Al Mahmud³, Kamrun Nahar⁴, Masayuki Fujita⁵, Mirza Hasanuzzaman^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 353-372, 2021, DOI:10.32604/phyton.2021.013259

Abstract Drought is the most common form of abiotic stress that reduces plant growth and productivity. It causes plant injuries through elevated production of reactive oxygen species (ROS). Potassium (K) is a vital plant nutrient that notably ameliorates the detrimental effect of drought stress in the plant. A pot experiment was conducted at the Laboratory of Plant Stress Responses, Faculty of Agriculture, Kagawa University, Japan, under controlled environment of green house to explore the role of K in mitigating drought severity in wheat (Triticum asevitum L.) seedlings. Three days after germination, seedlings were exposed to three water regimes viz., 100, 50,… More >

Hira Javaid Siddiqui¹, Shamim Gul^1,2,*, Attiq-ur-Rehman Kakar³, Umbreen Shaheen⁴, Gul Bano Rehman¹, Naqeebullah Khan³, Samiullah³

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 651-676, 2021, DOI:10.32604/phyton.2021.011413

Abstract This pot-based study investigated the influence of poultry manure and 1:1 mixture of poultry manure + biochar (produced from farmyard manure [FYM] or wood), on the biomass production and concentration of heavy metals in leaves of lettuce and spinach. The concentration of mineral nitrogen (N) and soluble inorganic phosphorus (P) of soils cultivated with these vegetables was also investigated. The application of poultry manure or FYM biochar in soil as 10% (equivalent to 60 t ha^–1 , an estimated 1726.8 kg ha^–1 N in poultry manure and 1353.9 kg ha^–1 N in FYM) and 15% amendment (equivalent to 90 t… More >

Zahoor Ahmad^1,2,*, Ejaz Ahmad Waraich³, Muhammad Zia ur Rehman⁴, Muhammad Ashar Ayub⁴, Muhammad Usman⁴, Hesham Alharby⁵, Atif Bamagoos⁵, Celaleddin Barutçular¹, Muhammad Ali Raza⁶, Fatih Çiğ⁷, Ayman El Sabagh^7,8,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 503-514, 2021, DOI:10.32604/phyton.2021.013588

Abstract Water is essential for the growth period of crops; however, water unavailability badly affects the growth and physiological attributes of crops, which considerably reduced the yield and yield components in crops. Therefore, a pot experiment was conducted to investigate the effect of foliar phosphorus (P) on morphological, gas exchange, biochemical traits, and phosphorus use efficiency (PUE) of maize (Zea mays L.) hybrids grown under normal as well as water deficit situations at the Department of Agronomy, University of Agriculture Faisalabad, Pakistan in 2014. Two different treatments (control and P @ 8 kg ha⁻¹ ) and four hybrids (Hycorn, 31P41, 65625,… More >

Yasmin Vázquez-Santos¹, Yuriana Martínez-Orea¹, Javier Álvarez-Sánchez¹, Noé Manuel Montaño², Sara Lucía Camargo-Ricalde², Silvia Castillo-Argüero^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 605-619, 2021, DOI:10.32604/phyton.2021.014208

Abstract The aim of this study was to analyze the performance of Acaena elongata colonized by arbuscular mycorrhizal fungi (AMF) to different phosphorus (P) concentrations, as a measure of AMF dependency. A. elongata, is a species from soils where P availability is limited, such as temperate forests. Our research questions were: 1) How do different P concentrations affect the AMF association in Acaena elongata, and 2) How does the AMF association influence A. elongata’s growth under different P concentrations? A. elongata’s growth, P content in plant tissue, AMF colonization and dependency were measured under four P concentrations: control (0 g P… More >