ALICIA I. WEYERS, LAURA I. UGNIA, HUGO GARCÍA OVANDO, NORA B. GORLA*

BIOCELL, Vol.26, No.2, pp. 225-228, 2002, DOI:10.32604/biocell.2002.26.225

Abstract Ciprofloxacin (CFX) is an effective and relatively safe antimicrobial used in a variety of human infections. However, adverse drug reactions and positive results in genotoxic tests are reported.
In order to understand the possible pathophysiological mechanisms of the toxic effects informed for CFX, lipid hydroperoxides (LOOH) -oxidative mediators of peroxidation- were quantified in liver and kidney of mice, after 15 to 360 minutes of the ciprofloxacin administration at doses of 10 mg/ Kg or 100 mg/ Kg by ip route. The peroxidation in the lipid fraction was evaluated by measuring the amount of hydroperoxides through the oxidation of 1-… More >

Gabriela MALANGA, Paula Mariela GONZÁLEZ, Juan Manuel OSTERA, Susana PUNTARULO^*

BIOCELL, Vol.40, No.1, pp. 35-38, 2016, DOI:10.32604/biocell.2016.40.035

Abstract The harmful effects of the reactive species may be due to the increase in their steady state concentration either by the enhancement of their production rates and/or the decrease of their consumption rate by antioxidant activity. The ascorbyl radical (A^• ) can be considered as a final product of radical oxidative transformations of ascorbate (AH^-). The ratio A^• content/AH^- content (A^• /AH^-) has been widely used as an interesting tool to estimate mild to moderate oxidative transformations, providing a quick and simple method of diagnosis of stress in the hydrophilic cellular medium. The aim of this work was to summarize… More >

DANIELA CARVALHO DOS SANTOS, ELISA APARECIDA GREGÓRIO

BIOCELL, Vol.26, No.2, pp. 229-235, 2002, DOI:10.32604/biocell.2002.26.229

Abstract The ultrastructure of the ovariole sheath along the Diatraea saccharalis ovariole was studied by scanning and transmission electron microscopy. Each ovariole is surrounded by an epithelial sheath, a tunica propria and scattered lumen cells. These three components of the ovariole sheath show different ultrastructural features along the ovariole, in the germarium or in the vitellarium; these differences are more evident in the epithelial sheath cells. The epithelial sheath is composed by two layers of cells, the external one running longitudinally and the internal one running circularly in the ovariole. These cells, in vitellarium, present cytoplasmic bundles of myofilaments that are… More >

LIDIA M. LOPEZ^*, AMANDA PELLEGRINO DE IRALDI^*, PATRICIA H. CARRIZO^**, MARTA DUBIN^**, ANDRÉS O.M. STOPPANI^**

BIOCELL, Vol.26, No.2, pp. 237-245, 2002, DOI:10.32604/biocell.2002.26.237

Abstract CG 10-248 (3,4-dihydro-2,2 dimethyl-9-chloro-2H-naphtho[1,2b]pyran-5,6-dione), a ß-lapachone analogue, modified the ultrastructure of rat liver mitochondria in vitro, in the absence of added oxidizable substrates. The condensed mitochondrial state was replaced by the orthodox or swollen state to a significant degree. The number of modified mitochondria depended on incubation time and quinone concentration, in the 25-100 µM range. Under the same experimental conditions, mitochondrial respiration was uncoupled as indicated by the increase in the rate of succinate oxidation by controlled mitochondria in metabolic state “4” (not in state “3”), and by the activation of latent F₀ F₁ -ATP synthase. Taking into account… More >

JUAN J. LÓPEZ-COSTA, CAROLINA ACUIPIL, PATRICIA TAGLIAFERRO, SAMEER PATEL, JAVIER RAMOS, ALICIA BRUSCO, JORGE PECCI SAAVEDRA*

BIOCELL, Vol.26, No.2, pp. 247-252, 2002, DOI:10.32604/biocell.2002.26.247

Abstract NADPH-diaphorase is a useful technique to reveal NO producing neurons at light microscopic level (LM). A modification of the technique using the tetrazolium salt BSPT as susbtrate, is useful to study the ultrastructure of NO neurons. The aim of this work was to perform a detailed analysis of NADPH diaphorase reactive neurons in rat mesencephalon both at light and electron microscopic levels.
NADPH-diaphorase reactive neurons were observed in superior colliculus, in central gray matter, in dorsal and medial raphe and in the pedunculopontine tegmental nucleus using two histochemical techniques at LM. Electron microscopy showed deposits on membranes of the… More >

ARTURO D. JORGE^*, LUIS S. GUTIERREZ^**, OLIVER JORGE^*, MARIO H. BURGOS^**

BIOCELL, Vol.26, No.2, pp. 253-262, 2002, DOI:10.32604/biocell.2002.26.253

Abstract The aim of this paper is to establish whether there are cytochemical or ultrastructural alterations in the hepatocytes of patients with primary biliary cirrhosis (PBC) at stages I and II compared with the biopsies from individuals with normal liver. Cytochemical technique with ATP as substrate, transmission electron microscopy (TEM) and freeze fracture were used for the studies.
In the normal liver biopsies the ultrastructural cytochemical localization of the enzymatic activity was clearly shown in the bile canaliculi. In the PBC biopsies, the enzymatic activity is increased in the bile canaliculi and is also present in the lateral membranes of… More >

Analia G. Karadayian¹, Juanita Bustamante², Silvia Lores-Arnaiz^{1, *}

BIOCELL, Vol.40, No.1, pp. 31-34, 2016, DOI:10.32604/biocell.2016.40.031

Abstract Alcohol hangover (AH) is defined as the temporary state after alcohol binge-like drinking, starting when EtOH is absent in plasma. Results from our laboratory have shown behavioral impairments and mitochondrial dysfunction in an experimental model of AH in mice. Our model consisted in a single i.p. injection of EtOH (3.8 g/kg BW) or saline solution in male and female mice, sacrificing the animals 6 hours after injection. Motor and affective behavior together with mitochondrial function and free radical production were evaluated in brain cortex and cerebellum during AH. Results showed that hangover animals exhibited a significant reduction in neuromuscular coordination,… More >

MARÍA TERESA PONCE, MÓNICA ELIZABETH GUIÑAZÚ, RICARDO TIZIO

BIOCELL, Vol.26, No.2, pp. 263-266, 2002, DOI:10.32604/biocell.2002.26.263

Abstract The goal of this study was to determine the effect of putrescine, added to the culture medium, on the in vitro development of stenospermic grape (Vitis vinifera L) embryos. The cross breedings of Perlón x G.C88552 and Perlón x Argentina were used. 0 (control), 2 and 4 mM of putrescine were added to the immature seed’s culture medium. In Perlón x Argentina, 2mM of putrescine statistically increased the percentage of total embryos, direct germination, polyembryos and normal plants. In Perlón x G.C88552, only 2 mM of putrescine increased all the variables considered, eventually tripling the percentage of normal plants obtained.… More >

Afet Golayoğlu Fatullayev¹, Canan Köroğlu²

CMES-Computer Modeling in Engineering & Sciences, Vol.86, No.1, pp. 39-52, 2012, DOI:10.3970/cmes.2012.086.039

Abstract In this work we solve numerically a boundary value problem for second order fuzzy differential equations under generalized differentiability in the form y''(t) = p(t)y'(t) + q(t)y(t) + F(t) y(0) = γ, y(l) = λ where t ∈T = [0,l], p(t)≥0, q(t)≥0 are continuous functions on [0,l] and [γ]^α = [γ_{_α},γ⁻_α], [λ]^α = [λ_{_α},λ^¯_α] are fuzzy numbers. There are four different solutions of the problem (0.1) when the fuzzy derivative is considered as generalization of the H-derivative. An algorithm is presented and the finite difference method is used for solving obtained problems. The applicability of presented… More >

Darío E. IGLESIAS^*, Silvina S. BOMBICINO, Alberto BOVERIS, Laura B. VALDEZ

BIOCELL, Vol.40, No.1, pp. 27-30, 2016, DOI:10.32604/biocell.2016.40.027

Abstract Complex III plays a central role in the mitochondrial respiratory chain transferring electrons from ubiquinol to cytochrome c and pumping protons to the intermembrane space, contributing to the protonmotive force. Furthermore, complex III can act as a source of O₂ •- in the presence of ubiquinol and antimycin, an expermiental condition in which the oxidation of the cytochrome b hemes is blocked. The O₂ •- dismutation catalyzed by superoxide dismutase produces H₂O₂, a known second messenger in redox signalling. Results from our laboratory have shown that NO, released from GSNO or from SPER -NO or generated by mtNOS, inhibits electron… More >