Wenjie Wei, Yulan Hu, Wenjuan Yang, Xiaoli Li, Jiali Wei, Xiangyang Hu, Ping Li^*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1075-1087, 2021, DOI:10.32604/phyton.2021.016134 - 27 April 2021

Abstract Seed germination or dormancy status is strictly controlled by endogenous phytohormone and exogenous environment signals. Abscisic acid (ABA) is the important phytohormone to suppress seed germination. Ambient high temperature (HT) also suppressed seed germination, or called as secondary seed dormancy, through upregulating ABI5, the essential component of ABA signal pathway. Previous result shows that appropriate nitric oxide (NO) breaks seed dormancy through triggering S-nitrosoglutathion reductase (GSNOR1)-dependent S-nitrosylation modification of ABI5 protein, subsequently inducing the degradation of ABI5. Here we found that HT induced the degradation of GSNOR1 protein and reduced its activity, thus accumulated more… More >

Stefan M. Kolašinac¹, Zora P. Dajić Stevanović^1,*, Sofija N. Kilibarda¹,  Aleksandar Ž. Kostić^2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1041-1062, 2021, DOI:10.32604/phyton.2021.015996 - 27 April 2021

Abstract Carotenoids represent a large group of mainly red, orange, and yellow natural metabolites mainly involved in regulation of many metabolic processes. Carotenoids are beneficial for human health. Current study describes the importance, chemical composition and functioning of carotenoids. It is well known that carotenoids support pigments acting in light absorbance mechanisms during photosynthesis, and are known to protect the chlorophyll molecules from oxidative stress and reactive oxygen species (ROS) damage. Carotenoids are involved in signaling processes in plants, responses to environmental stresses, pollination, germination and reproduction, and development regulation. As nutrients of strong antioxidant activity… More >

Min Yan¹, Han Wang¹, Shaohua Liu¹, Ye Xu¹, Zizhuo Nie¹, Yifeng Zhou², Li Li^2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1293-1300, 2021, DOI:10.32604/phyton.2021.015601 - 27 April 2021

Abstract Rho-related GTPase from plants (ROP) proteins play an essential role in plant stress resistance. In this study, the full-length GlROP6 gene was cloned based on G. littoralis transcriptome sequencing data acquired in response to salt stress. The protein sequence, conserved domains, secondary structure, three-dimensional structure, phylogenetic relationships, and expression pattern of the GlROP6 gene were systematically analysed. Our results showed that the full-length GlROP6 gene had an open reading frame of 606 bp, which encoded 201 amino acid residues with a relative molecular weight of 22.23463 kDa and a theoretical isoelectric point of 9.06. Amino acid sequence analyses… More >

Linjun Cai^1,#, Junhua Liu^2,#, Han Yun¹, Dan Du¹, Xiaolong Zhong¹, Zhenlin Yang¹, Xianchun Sang¹, Changwei Zhang^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1103-1117, 2021, DOI:10.32604/phyton.2021.015532 - 27 April 2021

Abstract Leaf color mutants are ideal materials for studying many plant physiological and metabolic processes such as photosynthesis, photomorphogenesis, hormone physiology and disease resistance. In this study, the genetically stable yellow-green leaf mutant ygl16 was identified from mutated “Xinong 1B”. Compared with the wild type, the pigment concentration and photosynthetic capacity of the ygl16 decreased significantly. The ultrastructural observation showed that the distribution of thylakoid lamellae was irregular in ygl16 chloroplasts, and the grana and matrix lamellae were blurred and loose in varied degrees, and the chloroplast structure was disordered, while the osmiophilic corpuscles increased. The results of More >

Shuo Qu¹, Yaolei Jiao², Lamboro Abraham², Piwu Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1177-1192, 2021, DOI:10.32604/phyton.2021.015206 - 27 April 2021

Abstract The fatty acid dehydrogenase gene plays an important role in regulating the oleic acid content in soybean. Genome-wide association study screened out soybean oleic acid related gene Gm15G117700. A fragment size of 693bp was obtained by PCR amplification of the gene and, it was connected by seamless cloning technology to the pMD18T cloning vector. Based on the gene sequence cloned, bioinformatic analysis of gene protein was performed. The overexpression vector of Gm15G117700 and the CRISPR/Cas9 gene editing vector were constructed. The positive plants were obtained by Agrobacterium-mediated transformation of soybean cotyledon nodes and T₂ plants were… More >

Menghua Xiao¹, Yuanyuan Li^1,2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1131-1146, 2021, DOI:10.32604/phyton.2021.015093 - 27 April 2021

Abstract The flooding caused by heavy rainfall in rice irrigation area and the drought caused by the drop of groundwater level are the research focus in the field of irrigation and drainage. Based on the comparative experiment and farmland water level control technology, this paper studied the average soil temperature under different soil layers (TM), the daily temperature change (TDC), the photosynthetic accumulation of single leaf and canopy in rice, and response of photothermal energy to rice root characteristics and growth factors in the paddy field under drought conditions. The results showed that the peak soil… More >

Simei Yao^1,2, Yu Jin^2,3, Limin Zhang², Ningfei Lei^1,*, Wei Xue^2,*, Jinsong Chen³, Qian Zhang², Feihai Yu^1,2

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1259-1271, 2021, DOI:10.32604/phyton.2021.014968 - 27 April 2021

Abstract Soil nutrients are commonly heterogeneously distributed and earthworms are one of the most common soil organisms. While effects of both soil nutrient heterogeneity and earthworms have been well studied, their interactive effect on plant community productivity has rarely been tested. In a greenhouse experiment, we constructed experimental plant communities by sowing seed mixtures of four grasses, two legumes and two forbs in either a heterogeneous soil consisting of low and high nutrient soil patches or a homogeneous soil where the low and high nutrient soil patches were evenly mixed. The earthworm Eisenia fetida was either added… More >

Kangning Zhou¹, Yingying Zhang¹, Jiasen Wu¹, Chunying Dou^1,2, Zihao Ye¹, Zhengqian Ye^1,3,*, Weijun Fu^1,3,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1217-1232, 2021, DOI:10.32604/phyton.2021.014951 - 27 April 2021

Abstract

The hyperaccumulator Sedum alfredii Hance (S. alfredii) may be employed for zinc (Zn) and cadmium (Cd)-polluted soil remediation. However, the low phytoremediation efficiency, related to the low biomass production, limits its use with that purpose. In this experiment, nitrogen (N), phosphorus (P), and potassium (K) fertilizers, and organic manure were applied to investigate the phytoremediation ability of S. alfredii. Hydroponic and pot experiments were conducted using Zn-Cd polluted soil. The hydroponic experiment indicated that appropriate fertilizer application could increase (p < 0.05) the amount of accumulated Zn and Cd in S. alfredii. When N supply ranged from 0.5 to 2.5

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Xiaoli Zhang^1,2,#, Xiaohong Yin^2,3,#, Jiana Chen^3,#, Fangbo Cao³, Yu Liu³, Zhengwu Xiao³, Liqin Hu³, Guanghui Chen³, Tianfeng Liang^1,2,*, Min Huang^3,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1285-1292, 2021, DOI:10.32604/phyton.2021.014778 - 27 April 2021

Abstract Protein in rice grains is an important source of nutrition for rice consumers. This study mainly aimed to identify the critical factors that determine grain protein concentration in rice. Accumulation parameters, including mean accumulation rate (R_mean) and active accumulation duration (D_active), for protein and non-protein components and their correlations with protein concentration in rice grains were investigated in field experiments conducted over two years with six rice cultivars. Results showed that grain protein concentration ranged from 9.6% to 11.9% across cultivars and years. Accumulation processes of protein and non-protein components were well fitted by the logistic More >

Yanfei Bi¹, Bin Wei¹, Ying Meng², Zhongzhao Li², Zhenghui Tang¹, Feng Yin³, Chuntao Qian^2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1161-1175, 2021, DOI:10.32604/phyton.2021.014396 - 27 April 2021

Abstract Melon (Cucumis melo), belonging to the Cucurbitaceae family, is a globally important economic crop. GRAS (GAI, RGA, SCR) genes, which are a type of transcription factor, play a critical role in plant growth and development, including processes such as radial root patterning, light signalling, abiotic/biotic stress, axillary shoot meristem formation, and phytohormone (gibberellin) signal transduction. In this study, the GRAS family in melon was analysed comprehensively with respect to chromosomal location, motif prediction, gene structure, and expression pattern. A total of 37 GRAS genes were first identified in melon, after which a phylogenetic tree was built… More >