KAN OUYANG^1,2,#, MEIQUAN XU^3,#, YUJIE LIANG⁴, XIAO XU², LIMEI XU², CAINING WEN^1,2, ZHUAN QIN², YIXIN XIE², HUAWEI ZHANG⁵, LI DUAN^2,*, DAPING WANG^1,2,5,*

BIOCELL, Vol.46, No.6, pp. 1521-1526, 2022, DOI:10.32604/biocell.2022.018788

Abstract There is no efficient tracking system available for the therapeutic molecules delivered to cartilage. The dense matrix covering the cartilage surface is the main biological barrier that the therapeutic molecules must overcome. In this study, we aimed to establish a system that can dynamically and effectively track the therapeutic molecules delivered to cartilage. To this aim, we adopted bovine and human cartilage explants as ex vivo models for chondrocyte-targeted exosome dispersion. The efficiency of drug delivery was evaluated using frozen sections. The results of this study showed that the penetration and distribution of chondrocyte-targeted exosomes in cartilage explants can be… More >

W. BENTON SWANSON, YUJI MISHINA^*

BIOCELL, Vol.46, No.6, pp. 1445-1451, 2022, DOI:10.32604/biocell.2022.018781

Abstract Mesenchymal stem cells (MSCs) have long been regarded as critical components of regenerative medicine strategies, given their multipotency and persistence in a variety of tissues. Recently, the specific role of MSCs in mediating regenerative outcomes has been attributed (in part) to secreted factors from transplanted cells, namely extracellular vesicles. This viewpoint manuscript highlights the promise of cell-derived extracellular vesicles as agents of regeneration, enhanced by synergy with appropriate biomaterials platforms. Extracellular vesicles are a potentially interesting regenerative tool to enhance the synergy between MSCs and biomaterials. As a result, we believe these technologies will improve patient outcomes through efficient therapeutic… More >

HE LI¹, RUNAN CHEN¹, ZHONGCHENG CHEN¹, JIAXIN LIN¹, XIJUN JIN¹, CHUNYUAN REN¹, QIUSEN CHEN¹, FENGQIONG CHEN¹, GAOBO YU^1,*, YUXIAN ZHANG^1,2,*

BIOCELL, Vol.46, No.6, pp. 1575-1592, 2022, DOI:10.32604/biocell.2022.018762

Abstract The Type-B authentic response regulator (ARR-Bs) gene family is one of the important plant-specific transcription factor families involved in variety of physiological processes. However, study of ARR-Bs gene family in soybean is limited. Genome-wide analysis and expression profiling of the ARR-Bs gene family were performed in the soybean genome. 31 ARR-Bs genes (namely GmARR-B1-31) were identified, containing conserved catalytic domains with protein lengths and molecular weights ranging from 246 to 699 amino acids (aa) and 28.30 to 76.86 kDa, respectively. Phylogenetic analysis grouped ARR-Bs genes into three clusters—Cluster I, Cluster II, and Cluster III—which included 15, 12, and 4 genes,… More >

YASHVI SHARMA¹, SHARDA RAY², SUJATA MOHANTY^1,*

BIOCELL, Vol.46, No.6, pp. 1435-1438, 2022, DOI:10.32604/biocell.2022.018612

Abstract Neurodegenerative disorders are a vicious woe to the public health and wellness. Uncertainty in their underlying causes, lack of effective biomarkers for their early detection, existence of only supportive therapy, and their ever rising incidence creates an unmatched need for targeted therapies. Mesenchymal Stem Cells (MSCs) have found to be promising candidates for regenerative and remedial therapy in neurodegenerative disorders, however several biological risks and practical issues impede in their translational utility. Deriving from MSCs are certain Extracellular Vesicles (EVs), which aid in the paracrine action of MSCs and have lately gained the scientific interest for their implacability in diverse… More >

JINGJIE LIU^1,#, XIAOYAN DING^2,#, LI XIANG¹, SHENGLI HUANG^3,*

BIOCELL, Vol.46, No.6, pp. 1537-1544, 2022, DOI:10.32604/biocell.2022.018441

Abstract This study aimed to evaluate the integration of transplanted choroidal plexus epithelial cells with organotypic spinal cord slices. Organotypic spinal cord slices, normally cultured for 6 days, were divided into control group (Ctrl) and transplanted group (T). The choroidal plexus epithelial cells were dissociated and primary cultured (C group). The choroidal plexus epithelial cells cultured for 6–7 days were labeled by 1,1’-dioctadecyl-3,3,3’,3’-tetramethyl-indocarbocyanineperchlorate (CM-Dil), and were identified by transthyretin (TTR) in immunocytochemistry. They were adjusted to the density of 0.5–1 × 10⁷/ml, then 2 μl cells suspension were transplanted to the spinal cord slices in the T group. The same amount… More >

BENSHUAI YOU¹, HUI QIAN^1,2,*

BIOCELL, Vol.46, No.6, pp. 1459-1463, 2022, DOI:10.32604/biocell.2022.018378

Abstract Exosomes, especially from mesenchymal stem cells, have attracted extensive attention in regeneration medicine. Mesenchymal stem cells derived exosomes (MSCs-exosomes) have shown anti-inflammatory, anti-oxidant, anti-apoptosis and tissue regeneration effects in a variety of tissue injury repair models. MSCs-exosomes hold many excellent properties such as low immunogenicity, biocompatibility, and targeting capability. With the in-depth study on the generation and function of exosomes, MSCs-exosomes are considered to be the bright stars in the field of regenerative medicine. However, there are still many obstacles to overcome in terms of exosomes isolation, clinical trials and safety evaluation. In this article, what we should focus on… More >

YINGHUI WU^#, HONG ZHANG^#, QIANG WANG, SUOYUAN LI, JUN SHEN^*

BIOCELL, Vol.46, No.6, pp. 1363-1364, 2022, DOI:10.32604/biocell.2022.018360

Abstract Transcription factor Brachyury, a protein containing 435 amino acids, has been widely investigated and reported in notochord differentiation and nucleus pulposus development. The crucial functions and underlying mechanisms by Brachyury are discussed in this paper, which suggests Brachyury can be developed into a potential novel target for the therapy of intervertebral disc degeneration. More >

YANQIU LI^#,*, CHENJUN HAO^#, WEIYI CHEN, QINGJU MENG

BIOCELL, Vol.46, No.6, pp. 1565-1573, 2022, DOI:10.32604/biocell.2022.018347

Abstract This work aimed to clarify the interaction between the fetus and pregnant patients with gestational diabetes mellitus (GDM), the lipid metabolomics analysis of the fetal umbilical cord blood of GDM patients and normal pregnant women were performed to screen out the specific lipid metabolites for pathogenesis of GDM. From 2019–2020, 21 patients with GDM and 22 normal pregnant women were enrolled in Hexian Memorial Hospital, Panyu District, Guangzhou. The general information such as weight, height, age, body mass index (BMI) before pregnancy were analyzed. Non-targeted metabonomic detection and analysis were performed in umbilical cord plasma using LC-MS method. The age,… More >

YANG YANG^1,*, BENSHUAI YOU², CHENGLIN ZHOU^1,*

BIOCELL, Vol.46, No.6, pp. 1557-1564, 2022, DOI:10.32604/biocell.2022.018326

Abstract The gene of Helicobacter pylori can encode three to four type IV secretory systems, of which a new gene region has been found in the H. pylori plasticity region. The coding products of this region can form a new T4SS named tfs3, but its function is unclear. This study investigated the effect of VirD4 recombinant protein in the tfs3 secretory system of the H. pylori clinical strain SBK on GES-1 cells. We observed changes in cell morphology after VirD4 treatment. Further analysis indicated that VirD4 increased inflammation by increasing the activation of NF-κB. VirD4 can also inhibited proliferation, and induced… More >

NING LI^1,#, YANG CHEN^2,#, YONGJIE YANG^3,4, SHUHAN LYU¹, YUE PAN^1,5,*

BIOCELL, Vol.46, No.6, pp. 1473-1482, 2022, DOI:10.32604/biocell.2022.018278

Abstract Breast cancer is a highly aggressive cancer in females. Metastasis is a major obstacle to the efficient and successful treatment of breast cancer. Cetyltrimethylammonium bromide (CTAB) has anti-tumor effects on a variety of tumors. We showed that CTAB inhibits the metastasis of breast cancer to the lungs both in vitro and in vivo. Epithelial-mesenchymal transition (EMT) is thought to be one of the major processes mediating breast cancer metastasis. We found that CTAB suppressed EMT and regulated the levels of the classical EMT markers E-cadherin, N-cadherin, vimentin, Snail and Twist1. Moreover, as a candidate anti-tumor agent, CTAB showed primary safety… More >