CRISTIAN ACOSTA, MABEL FÓSCOLO, JUAN CARLOS CAVICCHIA^*

BIOCELL, Vol.41, No.2-3, pp. 63-66, 2017, DOI:10.32604/biocell.2017.41.063

Abstract The testis is a double gland comprised of the sperm-producing seminiferous tubules and a complex endocrine interstice. The former structures generate and release whole cells (exocrine aspect of the gland) while the latter synthesize and release androgens and related hormones. The testis also has poorly understood paracrine connexions. These connexions play an important role during spermatogenesis. A key molecule within these structures is β-actin. Therefore, the present study aims at examining the expression pattern of β-actin during the various stages of the spermatic cycle in the rat. To achieve this goal, we used a combination of trans-illumination assisted microdissection of… More >

Xin ZM^1,2, MH Liu², Q Lu^1,3, CA Busso⁵, YJ Zhu^1,3, Z Li², YR Huang², XL Li2, FM Luo², F Bao¹, JQ Qian^4*, YH Li^1,3*

Phyton-International Journal of Experimental Botany, Vol.87, pp. 144-155, 2018, DOI:10.32604/phyton.2018.87.144

Abstract Leaf δ¹³C is widely used to explain plant strategies related to resource availability in different environments. However, the coupled response of leaf δ¹³C to precipitation and temperature as well as the relationship between leaf δ¹³C and leaf traits remain unclear. The leaf δ¹³C and its relationship with leaf traits [leaf size (LS), leaf length (LL), leaf width (LW), leaf length to width ratio (L:W), specific leaf area (SLA) and mass-based leaf nitrogen concentration (N_mass)] were investigated on the dominant shrub species Nitraria tangutorum Bobr. in the arid region (Dengkou and Minqin) of northwestern China under the simulated increasing precipitation (PGS)… More >

EDUARDO KOCH^1,2, ISRAEL A. VEGA^1,3,4, ALFREDO CASTRO-VAZQUEZ^1,3,4

BIOCELL, Vol.41, No.2-3, pp. 59-62, 2017, DOI:10.32604/biocell.2017.41.059

Abstract The digestive gland of the apple snail Pomacea canaliculata lodges two types of pigmented corpuscles (identified as C and K corpuscles) which has been proposed as endosymbiont/s. Both corpuscular types are always present in the digestive gland of adult snails, they are released into the tubuloacinar lumen and are later expelled in the feces. On their part, hatchlings lack any C or K corpuscles in the digestive gland as well as in their feces, whereas C corpuscles appear in both the gland and feces within one week after hatching. Hence, it is possible that the detritivorous hatchlings acquire the putative… More >

Jabbari H¹, M Gholamhosseini¹, M Naeemi², A Nasiri³

Phyton-International Journal of Experimental Botany, Vol.87, pp. 133-142, 2018, DOI:10.32604/phyton.2018.87.133

Abstract Two experiments were performed in 2011-2012 to investigate the response of three oilseed rape cultivars to drought stress, grown under two climate conditions in Iran (cold and hot climates). The experiments were conducted using a randomized complete-block design arranged in split-plot with three replicates. The irrigation treatments (FI: full irrigation; WIF: withholding irrigation at flowering stage, and WIS: withholding irrigation at the silique formation stage until physiological maturity) were allocated to main plots, whereas subplots consisted of the oilseed rape cultivars: early maturating (GKH2005), relatively late maturing (Opera) and late maturing (Okapi). Drought caused a significant reduction in seed number,… More >

Wang XG¹, Wuyunna^1*, CA Busso², YT Song¹, FJ Zhang¹, GW Huo¹

Phyton-International Journal of Experimental Botany, Vol.87, pp. 123-132, 2018, DOI:10.32604/phyton.2018.87.123

Abstract Chemical elements, such as carbon (C), nitrogen (N) and phosphorus (P), are major limiting nutrients in arid and semiarid grasslands and their stoichiometry (C:N:P) is a very important ratio to determine. In addition, it is critical to understand how plant stoichiometry responds to multiple environmental factors at the species level. In this study, we conducted a greenhouse experiment to investigate the effects of salt stress (4 g NaCl/kg soil), drought (35% of the soil water holding capacity) and N addition (10 g N/m²), as well as their interactions, on C, N and P concentrations and C:N:P ratios in two dominant… More >

Han DY^1,2, YF Yang²

Phyton-International Journal of Experimental Botany, Vol.87, pp. 113-122, 2018, DOI:10.32604/phyton.2018.87.113

Abstract Rapid and precise assessment of biodiversity is a central issue in conservation biology and biodiversity science. Higher taxonomic units and life form-based morphospecies were utilised to assess plant diversity patterns at the habitat scale in temperate meadow steppe, Songnen Plain, China. Patch area, taxonomic composition (families and genera) and life form (based on Raunkiaer’s life forms, and stem and root growth forms) were recorded in five communities. Taxonomic unit-based hierarchical diversity indices (HDI) and life form-based hierarchical diversity indices (HLDI) were calculated. Twenty-six families, 67 genera, and 87 species were recorded in the five communities. HDI and HLDI were significantly… More >

Espinoza-Sánchez EA¹, YA Sánchez-Peña², JA Torres-Castillo³, EA García-Zambrano², JE Treviño Ramírez², F Zavala-García², SR Sinagawa-García²

Phyton-International Journal of Experimental Botany, Vol.87, pp. 105-112, 2018, DOI:10.32604/phyton.2018.87.105

Abstract In this study, we established an in vitro regeneration system from immature embryos of four varieties of Sweet Sorghum with biotechnological potential. A total of 17 treatments were assesses in the induction of somatic embryogenic calli. We analyzed the effect of Murashige y Skoog media supplemented with different concentrations of plant growth regulators such as auxins and cytokinins. The callus induction response was evaluated using three immature embryos development stages and based on the number of embryos with callus induction. Different responses were observed in the embryos depending of the embryo age, culture condition, media composition and variety. The results… More >

Jimarez-Montiel MJ¹, A Robledo-Paz¹, VM Ordaz-Chaparro², LI Trejo-Tellez², JC Molina-Moreno¹

Phyton-International Journal of Experimental Botany, Vol.87, pp. 94-104, 2018, DOI:10.32604/phyton.2018.87.094

Abstract An alternative method for improving the production of habanero chili is the tissue culture technique; however, the gelling agent, the high salt and sucrose concentrations used in the culture media raise production costs and limit the adaptation of the regenerated plants to greenhouse or field conditions. In this study, the effect of the substrates perlite-coconut fiber, coconut fiber-volcanic rock, vermiculite-perlite, and perlite-volcanic rock in conjunction with various culture media in in vitro plant regeneration from embryos was evaluated. The differentiation of adventitious shoots on substrates was scarcely observed or non-existent. Inducing the formation of shoots on agar and their development… More >

Diego Masone^1,2, Aldana D. Gojanovich¹, Yesica R. Frontini-Lopez¹, Samanta DEL Veliz¹, Marina Uhart¹, Diego M. Bustos^1,3*

BIOCELL, Vol.41, No.2-3, pp. 55-58, 2017, DOI:10.32604/biocell.2017.41.055

Abstract In this study, we propose an automatic procedure for digital image processing. We describe a method that can efficiently quantify and characterize lipid droplets distributions in different cell types in culture. Prospectively, the lipid droplets detection method described in this work could be applied to static or time-lapse data, collected with a simple visible light or fluorescence microscopy equipment. Fully automated algorithms were implemented in Octave, a freely available scientific package. More >

LI KAILONG^1,2, DU XIAOLAN¹, HE YANI², ZHAO LIN¹, YANG JVRONG², SONG RUIHUA¹, CHEN LIN¹

BIOCELL, Vol.31, No.2, pp. 213-223, 2007, DOI:10.32604/biocell.2007.31.213

Abstract Objective: To investigate the course of tubular cell senescence and expressions of p53, p21, and Rb during the late phase of ischemia/reperfusion (IRI) in the kidney, and assess the effects of the p53-Rb pathway on tubular cell senescence. Methods: Experimental models of unilateral renal IRI were used in p53(+/+) and p53(-/-) mice. Histological changes at the tubular level, progress of cell senescence, and the expression of Rb, p21, and/or p53 proteins in tubular cells were studied at different moments in time after IRI. Results: Chronic tubulointerstitial fibrosis was much more severe and widely distributed in IRI kidneys of p53(+/+) mice… More >