Dairong LI¹, Xianlu ZHUO^1,2, Lumi HUANG¹, Xiaohui JI¹, Donglin WANG¹

BIOCELL, Vol.43, No.3, pp. 139-144, 2019, DOI:10.32604/biocell.2019.06460

Abstract X-ray repair cross-complementing protein 1 (XRCC1) could repair cisplatin-induced DNA damage. XRCC1 Arg399Gln and Arg194Trp variants alter XRCC1 expression and function, leading to changes in cancer sensitivity to cisplatin treatment. This study aimed to investigate the effects of XRCC1 Arg399Gln and Arg194Trp polymorphisms on cell viability, apoptosis and XRCC1 expression in cisplatin-sensitive A549 and cisplatin-resistant A549/DDP nonsmall cell lung cancer (NSCLC) cells. Plasmids carrying XRCC1 Arg399Gln and Arg194Trp were constructed and transfected into A549 and A549/DDP cells. RT–PCR, Western blot, MTT assay, and flow cytometry analysis were performed to assess cell viability, apoptosis, and XRCC1 expression. Compared to control cells,… More >

Grisel Fierros-Romero^1,*, M. Elena Mellado-Rojas², Reynaldo C. Pless³, Juan Ignacio Valiente-Banuet¹, Ashutosh Sharma¹ and Elda M. Beltrán-Peña²

Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 131-137, 2019, DOI:10.32604/phyton.2019.05036

Abstract Torin 1 is an ATP-competitive TOR inhibitor which inhibits the signaling of TOR and S6K kinase in mammals and plants. The objective of this research is to determine the effect of Torin 1 in a relatively simple and homogeneous plant system such as the NT-1 tobacco suspension cell cultures. Cultures of NT-1 cells were tested with 5, 50, 150 and 250 nM of Torin 1. During kinetics growth of NT-1 tobacco suspension cell cultures, 150 and 250 nM Torin 1 inhibits the early growth and later enhanced the cellular proliferation during exponential growth by means of an increased expression of… More >

Muhammad Zafar-ul-Hye^1,*, Tariq Shahzad Bhutta¹, Muhammad Shaaban¹, Shahid Hussain¹, Muhammad Farooq Qayyum¹, Umar Aslam¹ and Zahir Ahmad Zahir²

Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 119-129, 2019, DOI:10.32604/phyton.2019.06570

Abstract Soil salinity affects the growth and yield of crops. The stress of soil salinity on plants can be mitigated by inoculation of plant growth promoting bacteria (PGPR). The influence of PGPR inoculation on wheat (Triticum aestivum L.) crop productivity under salinity stress has not been properly addressed so far. Therefore, the present study was conducted to investigate the effects of various PGPR strains (W14, W10 and 6K; alone and combined) at several growth attributes of wheat plant under different soil salinity gradients (3, 6 and 9 dS m-1). The growth attributes of wheat (height, roots, shoots, spikes, grains quality, biological… More >

Wen JING^1,§, Chuanfen LI^2,3,§, Yingli LU⁴, Lianshi FENG⁴

BIOCELL, Vol.43, No.3, pp. 129-138, 2019, DOI:10.32604/biocell.2019.06087

Abstract This study aimed to investigate the microRNA expression profile and the characteristics of lipid metabolism in the livers of rats undergoing a high-fat diet. Fifty male Sprague-Dawley (SD) rats were divided into a standard chow group (C group, N = 10) and a high-fat diet group (H group, N = 40). After 12 weeks, the rat body weight, body length, fat mass, and serum lipid concentration were measured. The expression profile of microRNAs and the gene and protein expression levels involved in lipid metabolism in rat liver were detected. Body fat and serum lipid concentrations were all significantly higher in… More >

Qunqun Hao^1,#, Bo Lyu^2,#, Yuhan Tang^1,#, Deya Wang¹, Yuanyuan Li¹, Qingliang Li¹, Yuhai Wang^1,* and Wenqiang Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 109-117, 2019, DOI:10.32604/phyton.2019.06734

Abstract A barley mutant, 194, was observed to exhibit a leaf spot phenotype over the whole course of its growing period. In this study, the phenotype and antioxidant competence were studied in the lesion mimic mutant 194. Plant height was slightly higher in mutant 194 than in the wild type (WT). In addition, leaf spot per plant in mutant 194 was significantly higher than in WT. Antioxidant competence, as indicated by reactive oxygen species (ROS) accumulation, antioxidant enzyme activity, and the expression of antioxidant enzyme-encoding genes was also assessed in mutant 194. Compared to the WT, mutant 194 displayed a relatively… More >

Z.Y. Qian¹, Z.D. Han¹, S.N. Atluri¹

CMES-Computer Modeling in Engineering & Sciences, Vol.5, No.6, pp. 541-562, 2004, DOI:10.3970/cmes.2004.005.541

Abstract Novel non-hyper-singular [i.e., only strongly-singular] boundary-integral-equations for the gradients of the acoustic velocity potential, involving only O(r⁻²) singularities at the surface of a 3-D body, are derived, for solving problems of acoustics governed by the Helmholtz differential equation. The gradients of the fundamental solution to the Helmholtz differential equation for the velocity potential, are used in this derivation. Several basic identities governing the fundamental solution to the Helmholtz differential equation for velocity potential, are also derived. Using these basic identities, the strongly singular integral equations for the potential and its gradients [denoted here as φ-BIE, and q-BIE, respectively], are rendered… More >

Ji SHI; Peixin SUN, Ye ZHANG, Bing YAO, Haozhe PIAO

BIOCELL, Vol.43, No.3, pp. 119-128, 2019, DOI:10.32604/biocell.2019.07577

Abstract Glioma is the most common primary malignant brain tumor with a poor survival rate. In recent years, no significant progress has been made in the treatment of gliomas in contrast to the development of improved diagnosis via molecular typing. Newcastle disease virus (NDV), a negative-stranded RNA virus that exhibits oncolytic activity, has been investigated for its capacity to elicit antitumor activity in many types of cancers, including glioma. Therefore, application of oncolytic viruses, such as NDV, as a new treatment strategy to specifically target aberrant signaling in glioblastomas has brought new hope. For many years, NDV has been investigated for… More >

Min Huang*, Hengdong Zhang, Chunrong Zhao, Guanghui Chen and Yingbin Zou

Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 101-107, 2019, DOI:10.32604/phyton.2019.06694

Abstract Improving grain amino acid content of rice (Oryza sativa L.) is essential for the health of consumers. This study was conducted to identify the physiological processes that contribute to the higher grain amino acid content in hybrid rice cultivar Lingliangyou 268 compared to Luliangyou 996. The results showed that total amino acid content in grains was 9% higher in Lingliangyou 268 than in Luliangyou 996. There was no significant difference in grain nitrogen (N) content between Lingliangyou 268 and Luliangyou 996, while ratio of amino acid to N was 6% higher in Lingliangyou 268 compared to Luliangyou 996. A total… More >

Bengü ERGÜDEN

BIOCELL, Vol.43, No.3, pp. 103-118, 2019, DOI:10.32604/biocell.2019.06517

Abstract The expression of a gene is governed at various levels, from transcriptional to translational level. The translational control is widely used to regulate gene expression, especially when a rapid, local, and selective control over protein synthesis is required. The present review describes instructive examples of translational regulation in yeast, together with regulatory elements within mRNAs. The review also outlines the important contributions of mRNAbinding proteins that act in harmony with several translational elements to generate appropriate translational signals and responses. More >

Xiachen Lv^1,#, Yunxia Fang^1,#, Lantian Zhang¹, Weiyi Zhang¹, Ling Xu¹, Jingjin Han¹, Bailing Jin², Xian Zhang¹, Xiaoqin Zhang^1,*, Dawei Xue^1,*

Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 91-100, 2019, DOI:10.32604/phyton.2019.06622

Abstract Melatonin (MLT) is a hormonal substance found in many organisms and can improve plant stress resistance. In this study, the japonica rice variety Y32 and indica rice variety NJ6 were cultivated in hydroponics under different concentrations of CdCl₂ at the two-leaf stage. The growth, physiological and biochemical responses of the seedlings and the expression of cadmium (Cd)-related genes under exogenous melatonin (MLT) treatment were assessed. The results indicated that Cd stress destroyed the dynamic balance between reactive oxygen species (ROS) production and removal, resulting in ROS accumulation, membrane lipid peroxidation, and impaired growth and development. Following the application of exogenous… More >