Riaz Ahmad¹, Muhammad Akbar Anjum^1,*, Safina Naz¹, Rashad Mukhtar Balal²

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 17-34, 2021, DOI:10.32604/phyton.2020.011680

Abstract Selection and use of molecular markers for evaluation of DNA polymorphism in plants are couple of the most important approaches in the field of molecular genetics. The assessment of genetic diversity using morphological markers is not sufficient due to little differentiating traits among the species, genera or their individuals. Morphological markers are not only highly influenced by environmental factors but skilled assessment is also prerequisite to find the variations in plant genetic resources. Therefore, molecular markers are considered as efficient tools for detailed DNA based characterization of fruit crops. Molecular markers provide new directions to the efforts of plant breeders… More >

Wilber Montejo-Mayo¹, Eduardo Días-Rivera¹, Mauro Martínez-Pacheco², Abril Munro-Rojas¹, Enrique Ambriz-Parra¹, Crisanto Velázquez-Becerra^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 193-206, 2021, DOI:10.32604/phyton.2020.011402

Abstract Wood is an organic material that is a source of carbon of organisms called Wood-decay fungi, and to preserve the wood, various toxic compounds to man and the environment have been used. To analyze the effect of N,N-Dimethylhexadecylamine (DMHDA) on wood attacked by the rotting fungus Trametes versicolor L. We used an in vitro system to expose the fungus T. versicolor to different concentrations of the DMHDA (50, 150 and 450 μM). We quantified the diameter of mycelial growth and laccase activity, also, under these experimental conditions we studied morphological details of the organisms using different scanning equipment including scanning… More >

Tehmina Bahar^1,*, Adeeba Mahboob Qureshi¹, Fasiha Qurashi^1,2, Muniba Abid¹, Misbah Batool Zahra¹, Muhammad Saleem Haider¹

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 75-86, 2021, DOI:10.32604/phyton.2020.010597

Abstract Most damaging plant diseases have been caused by viruses in the entire world. In tropical and subtropical areas, the damage caused by plant virus leads to great economic and agricultural losses. Single stranded DNA viruses (geminiviruses) are the most perilous pathogens which are responsible for major diseases in agronomic and horticultural crops. Significantly begomoviruses and mastreviruses are the biggest genus of plant infecting viruses, transmitted though Bemisia tabaci and members of Cicadellidae respectively. Plants possesses some naturally existing chemicals term as phyto-chemicals which perform important functions in the plant. Some antioxidant enzymes are used by plants for self-defense upon foreign… More >

Guiming Deng^1,2,3, Ou Sheng^1,2,3, Fangcheng Bi^1,2,3, Chunyu Li^1,2,3, Tongxin Dou^1,2,3, Tao Dong^1,2,3, Qiaosong Yang^1,2,3, Huijun Gao^1,2,3, Jing Liu⁴, Xiaohong Zhong⁴, Miao Peng⁴, Ganjun Yi^1,2,3, Weidi He^1,2,3, Chunhua Hu^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 1101-1130, 2020, DOI:10.32604/phyton.2020.010970

Abstract Banana (Musa spp.) is an ancient and popular fruit plant with highly nutritious fruit. The pseudo-stem of banana represents on average 75% of the total dry mass but its valorization as a nutritional and industrial by-product is limited. Recent advances in metabolomics have paved the way to understand and evaluate the presence of diverse sets of metabolites in different plant parts. This study aimed at exploring the diversity of primary and secondary metabolites in the banana pseudo-stem. Hereby, we identified and quantified 373 metabolites from a diverse range of classes including, alkaloids, flavonoids, lipids, phenolic acids, amino acids and its… More >

Jiali Wei^*, Ying Zou, Ping Li, Xiaojun Yuan

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 883-891, 2020, DOI:10.32604/phyton.2020.010973

Abstract TiO₂ nanoparticles (nano-TiO₂) are widely used in the world, and a considerable amount of nano-TiO₂ is released into the environment, with toxic effects on organisms. In the various species of higher plants, growth, including seed germination, root elongation, and biomass accumulation, is affected by nano-TiO₂. However, the underlying molecular mechanisms remain to be elucidated. In this study, we observed that nano-TiO₂ promoted root elongation in a dose-dependent manner. Furthermore, we found that nano-TiO₂ elevated auxin accumulation in the root tips of the auxin marker lines DII-VENUS and DR5:: GUS, and, correspondingly, quantitative real-time PCR analysis revealed that nano-TiO₂ increased the… More >

Hyewon Kim¹, Chanwoo Park², Jong Hwan Lim², Hye Woo Shin^3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 1019-1033, 2020, DOI:10.32604/phyton.2020.010649

Abstract Flowering onset has attracted much attention in ecological research as an important indicator of climate change. Generally, warmer temperatures advance flowering onset. The effect of climate warming on flowering onset is more pronounced in spring because the difference between atmospheric and water temperatures creates more rapid convection than in other seasons. We analyzed the correlation between 73 species of spring woody plants in Hongneung Arboretum in Seoul, South Korea and the spring minimum temperature and average precipitation over the past 50 years (1968–2018). The spring minimum temperature and average precipitation have increased over the past 50 years, resulting in the… More >

Arnab Kumar De, Arijit Ghosh, Debabrata Dolui, Indraneel Saha, Malay Kumar Adak^*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 999-1017, 2020, DOI:10.32604/phyton.2020.010828

Abstract In the present experiment with ongoing concentration (0 µM, 100 µM, 250 µM, 500 µM and 1000 µM) of 2,4-D, the responses of Azolla pinnata R.Br. was evaluated based on cellular functions. Initially, plants were significantly tolerated up to 1000 µM of 2,4-D with its survival. This was accompanied by a steady decline of indole acetic acid (IAA) concentration in tissues with 78.8% over the control. Membrane bound H⁺ -ATPase activity was over expressed within a range of 1.14 to 1.25 folds with activator (KCl) and decreased within a range of 57.3 to 74.6% in response to inhibitor (Vanadate) application.… More >

Meiling Tang^1,2,#, Yuhe Li^1,3,#, Yahui Chen^1,4, Lei Han^1,3, Hongxia Zhang^1,3, Zhizhong Song^1,3,4,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 925-941, 2020, DOI:10.32604/phyton.2020.011184

Abstract As the preferred nitrogen (N) source, ammonium (NH₄⁺ ) contributes to plant growth and development and fruit quality. In plants, NH₄⁺ uptake is facilitated by a family of NH₄⁺ transporters (AMT). However, the molecular mechanisms and functional characteristics of the AMT genes in peach have not been mentioned yet. In this present study, excess NH₄⁺ stress severely hindered shoot growth and root elongation, accompanied with reduced mineral accumulation, decreased leaf chlorophyll concentration, and stunned photosynthetic performance. In addition, we identified 14 putative AMT genes in peach (PpeAMT). Expression analysis showed that PpeAMT genes were differently expressed in peach leaves, stems… More >

Yingting Zhang^1,2,3, Junjie Yang^1,2,3, Zhenhao Guo^1,2,3, Jiaxing Mo^1,2,3, Jiebing Cui^1,2,3, Hailiang Hu^1,2,3, Jin Xu^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 957-986, 2020, DOI:10.32604/phyton.2020.011211

Abstract Cryptomeria fortunei (Chinese cedar) is a highly adaptable woody species and one of the main forest plantation trees in subtropical high-altitude areas in China. However, there are few studies on its chloroplast (cp) genome. In this study, the complete cp genome of C. fortunei was sequenced and evaluated via comparative analyses with those of related species (formerly the Taxodiaceae) in Cupressaceae. The C. fortunei cp genome was 131,580 bp in length, and the GC content of the whole genome was 35.38%. It lost one relevant large inverted repeat and contained 114 unique genes, including 82 protein-coding genes, 28 tRNAs and… More >

Murad Awad¹, Photini V. Mylona², Alexios N. Polidoros^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 905-923, 2020, DOI:10.32604/phyton.2020.011235

Abstract Discrimination among grapevine varieties based on quantitative traits, such as flowering, veraison and ripening dates is crucial for variety selection in the context of climate change and in breeding programs. These traits are under complex genetic control for which 6 linked SSR loci (VVS2, VVIn16, VMC7G3, VrZAG29, VMC5G7, and VVIB23) have been identified. Using these markers in HRM-PCR analysis, we assessed genetic diversity among a large collection of 192 grapevine varieties. The grapevine germplasm used encompasses the majority of Greek vineyard with 181 varieties, 3 prominent foreign varieties and 11 varieties of Palestinian origin. The SSR markers used were highly… More >