Open Access

ARTICLE

Ameliorative effects of melatonin and zinc oxide nanoparticles treatment against adverse effects of busulfan induced infertility in male albino mice

AMOURA M. ABOU-EL-NAGA¹, SHAKER A. MOUSA², FAYEZ ALTHOBAITI³, EMAN FAYAD^3,*, ENGY S. FAHIM¹
1 Department of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt
2 The Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Rensselaer, USA
3 Department of Biotechnology, Faculty of Sciences, Taif University, Taif, Saudi Arabia
* Corresponding Author: EMAN FAYAD. Email:
(This article belongs to this Special Issue: Molecular and Cellular Nanobiotechnology)

BIOCELL 2022, 46(2), 535-545. https://doi.org/10.32604/biocell.2022.017739

Received 02 June 2021; Accepted 23 July 2021; Issue published 20 October 2021

Abstract

Testicular damage is one of the most hazardous effects as it’s associated with azoospermia. Busulfan (Bu) is a highly toxic chemotherapeutic drug that affects testis. Thirty male Swiss albino mice divided into six groups of 5 animals each. Control (oral 0.9% saline daily for 75 days); Mel (20 mg/kg/day orally for 30 days); ZnO NPs (5 mg/kg/day i.p. for 30 days); BU (single i.p. injection of 40 mg/kg and then left for 45 days); BU + Mel (single 40 mg/kg dose of BU and left for 45 days followed by 20 mg/kg/day Mel for 30 days); BU + ZnO NPs (single dose of 40 mg/kg of BU and left for 45 days, then 5 mg/kg/day ZnO NPs for 30 days). Preparation and Characterization of ZnO NPs. Specimens from testis prepared for ultrastructural investigations using TEM after Masson’s trichrome and toluidine blue staining. BU induced histological and ultrastructural damage of the testis. Moreover, the present results could be concluded that Mel or ZnO NPs can protect the testicular tissue against ultrastructural alterations induced by BU by its antioxidant and anti-apoptotic effects.

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Keywords

Busulfan; Melatonin; Zinc oxide nanoparticles; Ultrastructure; Testis; Mice

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Citations