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ARTICLE
Dithiothreitol and PEG Induced Combined Stress May Affect the Expressions of ABA Aldehyde Oxidase, Sucrose Synthase and Proline Metabolic Genes in Maize Seedlings
Cansu Altuntaş1, Rabiye Terzi1,*
1 Karadeniz Technical University, Department of Biology, Faculty of Science, Trabzon, 61080, Turkey
* Corresponding Author: Rabiye Terzi. Email:
(This article belongs to this Special Issue: Crop Production under Abiotic Stress: Physiological and Molecular Interventions)
Phyton-International Journal of Experimental Botany 2020, 89(3), 487-495. https://doi.org/10.32604/phyton.2020.08919
Received 24 October 2019; Accepted 06 December 2019; Issue published 22 June 2020
Abstract
The endoplasmic reticulum (ER) is an organelle in the cell where
proteins are created and folded. Folding is a very elaborate process that is often
interrupted by various biotic and abiotic stresses, leading to the formation of
unfolded and misfolded proteins called ER stress. Dithiothreitol (DTT)-induced
unfolded protein response (UPR) in endoplasmic reticulum (ER) has been
recently reported in plants. Also, previous studies demonstrated that treatment
with polyethylene glycol (PEG
6000) could stimulate water deficit in crops.
However, further researches should be conducted to elucidate the molecular
mechanism of ER stress response and the relationship between water deficiency
and ER. In this study, we examined the expressions of
sucrose synthase (
SuS)
gene, proline metabolic genes and
abscisic aldehyde oxidase (
AAO3) gene in
maize seedlings that were subjected to DTT and PEG induced combined stresses
by using quantitative real-time RT-PCR. Three weeks old detached maize
seedlings were treated with or without DTT and PEG
6000 for 12 h. The treatment
with DTT increased about 2-fold the expression of gene encoding proline
synthesis enzyme,
pyrroline-5-carboxylate synthetase (
P5CS) but no statistically
affected the proline catabolism enzyme,
proline dehydrogenase (
ProDH) in
comparison with un-treated seedlings. PEG treatment was also up-regulated
P5CS while it was down-regulated
ProDH. The relative expression levels of
SuS
and
AAO3 genes statistically enhanced about 2.5 fold under the DTT-induced ER
stress. Likewise, the expression levels of
SuS and
AAO3 genes were up-regulated
in the detached seedlings exposed to PEG-induced water deficit. Conversely, the
induced gene expressions were down-regulated under the combined stress, the
DTT-induced ER stress and PEG-induced water deficit in comparison with the
singular stress responses (DTT or PEG). The results indicated that the expressions
of genes, related to the synthesis of some signal osmolyte compounds such as
proline and sucrose can be suppressed when ER stress occurred under water
deficiency in maize seedlings. The changes in the expressions of genes involved in
osmolyte and ABA metabolism can be related to ER stress response as well as
variations in water status.
Keywords
Cite This Article
Altuntaş, C., Terzi, R. (2020). Dithiothreitol and PEG Induced Combined Stress May Affect the Expressions of ABA Aldehyde Oxidase, Sucrose Synthase and Proline Metabolic Genes in Maize Seedlings.
Phyton-International Journal of Experimental Botany, 89(3), 487–495. https://doi.org/10.32604/phyton.2020.08919
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