Open Access
ARTICLE
Overexpression of IbSINA5 Increases Cold Tolerance through a CBF SINA-COR Mediated Module in Sweet Potato
Shiyang Li1,#, Xue-Ao Liu2,#, Lizi Zhao2, Huiqing Huang3, Bei Li2, Zhizhong Song2, Meixia Liang2, Hongxia Zhang2,3, Limin Wang2,*, Shenglin Zhou1,*
1 Shandong Technology and Business University, Yantai, 264005, China
2 The Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai, 264025, China
3 School of Life Sciences, Ludong University, Yantai, 264025, China
* Corresponding Authors: Limin Wang. Email: ; Shenglin Zhou. Email:
Phyton-International Journal of Experimental Botany 2021, 90(3), 761-772. https://doi.org/10.32604/phyton.2021.016314
Received 20 February 2021; Accepted 05 March 2021; Issue published 30 March 2021
Abstract
Seven
in absentia (SINA) family proteins play a central role in plant growth, development and resistance to abiotic stress. However, their biological function in plant response to cold stress is still largely unknown. In this work, a
seven in absentia gene
IbSINA5 was isolated from sweet potato. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses demonstrated that
IbSINA5 was ubiquitously expressed in various tissues and organs of sweet potato, with a predominant expression in fibrous roots, and was remarkably induced by cold, drought and salt stresses. Subcellular localization assays revealed that IbSINA5-GFP fusion protein was mainly localized in cytoplasm and nucleus. Overexpression of
IbSINA5 in sweet potato led to dramatically improved resistance to cold stress in transgenic plants, which was associated with the up-regulated expression of
IbCOR (cold-regulated) genes, increased proline production, and decreased malondialdehyde (MDA) and H
2O
2 accumulation in the leaves of transgenic plants. Furthermore, transient expression of
IbCBF3, a C-repeat binding factor (CBF) gene, in the leaf protoplasts of wild type sweet potato plants up-regulated the expression of both
IbSINA5 and
IbCOR genes. Our results suggest that
IbSINA5 could function as a positive regulator in the cold signaling pathway through a CBF-SINA-COR mediated module in sweet potato, and have a great potential to be used as a candidate gene for the future breeding of new plant species with improved cold resistance.
Keywords
Cite This Article
Li, S., Liu, X., Zhao, L., Huang, H., Li, B. et al. (2021). Overexpression of
IbSINA5 Increases Cold Tolerance through a CBF SINA-COR Mediated Module in Sweet Potato.
Phyton-International Journal of Experimental Botany, 90(3), 761–772. https://doi.org/10.32604/phyton.2021.016314