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  • Open Access

    ARTICLE

    Exploration of Distinct Physiological and Biochemical Alterations in Chickpea (Cicer arietinum L.) under Varying Organic Materials, Drought and Proline Application

    Uttam Kumer Sarker1, A. N. Md. Anamul Karim1, F. M. Jamil Uddin1, Ahmed Khairul Hasan1, Najrul Islam1, Md. Romij Uddin1,*, Md. Alamgir Hossain2, Sabry Hassan3, Mohamed M. Hassan3, Mahmoud F. K. Soliman4

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2775-2789, 2022, DOI:10.32604/phyton.2022.023112 - 29 August 2022

    Abstract Chickpea yield is decreasing day by day due to drought stress, which could be an immense risk for future food security in developing countries. Management practices could be the most excellent approach to diminish loss due to this abiotic factor. The current research work was designed to explore the tolerance reaction of chickpea genotypes against management practices, through morphological and biochemical parameters and evaluate yield performance across drought prone location of Bangladesh. Four genotypes BD-6048, BD-6045, BD-6090, BD-6092 and eight management practices, e.g., severe water stress (SWS), i.e., without irrigation, 10 cm thick mulching with… More >

  • Open Access

    ARTICLE

    Breeding Enrichment of Genetic Variation of Grain Yield and Its Attributes in Bread Wheat under Drought Stress and Well Irrigation

    Dina Swelam1, Abdel Hamid Salem2, Manal Hassan1, Mohammed Ali2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2699-2717, 2022, DOI:10.32604/phyton.2022.022651 - 29 August 2022

    Abstract Drought stress (DS) is one of the most critical environmental abiotic stresses for wheat production in the arid environments. Selection of high-yielding genotypes tolerant to DS can play a significant role in mitigation the negative impacts associated with DS. In the present study, generation means analysis (GMA) was used to study the performance of two crosses under well irrigation (WI) and deficit irrigation [cross I (Line 44 × Shandweel-1) and cross II (Line 20 × Sakha 93)]. Significant differences were observed for days to heading (DH), days to maturity (DM), plant height (PH), spike length (SL), number of spikes… More >

  • Open Access

    ARTICLE

    Health Risks Assessment of Heavy Metal Pollution in the Soil-Crop System from an E-Waste Dismantling Area

    Shengting Rao#, Jia Fang#, Keli Zhao*

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2669-2685, 2022, DOI:10.32604/phyton.2022.022416 - 29 August 2022

    Abstract

    Soil is an essential resource for agricultural production. In order to investigate the pollution situation of heavy metals in the soil-crop system in the e-waste dismantling area, the crop and soil samples (226 pairs, including leaf vegetables, solanaceous vegetables, root vegetables, and fruits) around the e-waste dismantling area in southeastern Zhejiang Province were collected. The concentrations of Cd, Cu, Pb, and Cr were determined. The average concentrations of Cd, Cu, Pb, and Cr in soils were 0.94, 107.79, 80.28, and 78.14 mg kg-1, respectively, and their corresponding concentrations in crops were 0.024, 0.7, 0.041, and 0.06

    More >

  • Open Access

    ARTICLE

    Exploration of Genetic Pattern of Phenological Traits in Wheat (Triticum aestivum L.) under Drought Stress

    Iqra Ishaaq1, Muhammad Umer Farooq1,2,3,4,*, Syeda Anjum Tahira2, Rizwana Maqbool1, Celaleddin Barutçular3, Muhammad Yasir5, Saira Bano2, Zaid Ulhassan6, Ghassan Zahid7, Muhammad Ahsan Asghar8, Sajad Hussain9, Kocsy Gabor8, Ulkar Ibrahimova10,11, Jianqing Zhu4, Anshu Rastogi12,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2733-2758, 2022, DOI:10.32604/phyton.2022.022094 - 29 August 2022

    Abstract Drought is the major detrimental environmental factor for wheat (Triticum aestivum L.) production. The exploration of genetic patterns underlying drought tolerance is of great significance. Here we report the gene actions controlling the phenological traits using the line × tester model studying 27 crosses and 12 parents under normal irrigation and drought conditions. The results interpreted via multiple analysis (mean performance, correlations, principal component, genetic analysis, heterotic and heterobeltiotic potential) disclosed highly significant differences among germplasm. The phenological waxiness traits (glume, boom, and sheath) were strongly interlinked. Flag leaf area exhibits a positive association with peduncle… More >

  • Open Access

    ARTICLE

    The Effect of Methylation Modification of MDD on the Expression of Key Genes in the Process of Saponin Synthesis in Eleutherococcus senticosus

    Minghui Cui, Limei Lin, Jie Zhang, Xin Song, Shuo Wang, Jing Dong, Xuelei Zhao, Yuehong Long*, Zhaobin Xing*

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2649-2668, 2022, DOI:10.32604/phyton.2022.021631 - 29 August 2022

    Abstract Mevalonate pyrophosphate decarboxylase is a kind of key enzyme in the terpenoid synthesis pathway in Eleutherococcus senticosus. The results of bisulfite sequencing showed that there were three kinds of samples with a low (0.68%), medium (0.72%) and high (0.79%) DNA methylation ratio in the promoter of MDD in E. senticosus, respectively. The transcriptome sequencing results showed that the expression of MDD in E. senticosus was significantly up-regulated in the types with low DNA methylation ratios of MDD (P < 0.05). There was a significant negative correlation between the saponin content in E. senticosus and the DNA methylation ratio of MDD promoter (PMore >

  • Open Access

    ARTICLE

    Combination of 6-Benzylaminopurine and Thidiazuron Promotes Highly Efficient Shoot Regeneration from Cotyledonary Node of Mature Peanut (Arachis hypogaea L.) Cultivars

    Abraham Lamboro1,*, Xiao Han1, Songnan Yang1, Xueying Li1, Dan Yao2, Baixing Song1, Jun Zhang1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2619-2631, 2022, DOI:10.32604/phyton.2022.021404 - 29 August 2022

    Abstract Efficient in vitro plantlet regeneration is an important step to successfully transform genes for the improvement of agronomic traits. A combination of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) plant growth regulators was applied to evaluate shoot regeneration capacity whereas α-naphthalene acetic acid (NAA) combination with 6-benzylaminopurine (BAP), and 2, 4-dichlorophenoxyacetic acid (2, 4-D) with 6-benzylaminopurine were tested to optimize root induction for two peanut cultivars. The result showed combination (BAP with TDZ) was found to be effective in promoting shoot. The highest shoot regeneration frequency (93%) was obtained on a medium supplemented with 4 mg/L BAP and 0.5 mg/L… More >

  • Open Access

    REVIEW

    Heavy Metal/Metalloid Indexing and Balances in Agricultural Soils: Methodological Approach for Research

    Shahid Hussain*

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2687-2697, 2022, DOI:10.32604/phyton.2022.021158 - 29 August 2022

    Abstract Heavy metal(loid) accumulation in agricultural soils is a threat to the soil capacity, quality, and productivity. It also increases human exposure to heavy metal(loid)s via consumption of contaminated plant-based foods. The detrimental effects of soil contamination also deteriorate the environment of plants and animals. For sustainable agriculture, therefore, the soil must be protected from toxic levels of heavy metal(loid)s. Studies on heavy metal(loid) balances in agricultural soils are important in predicting future risks to sustainable production from agro-ecological zones and human exposure to heavy metal(loid)s. The latest and continuous indexing of the problem seems a More >

  • Open Access

    ARTICLE

    SPAD Value Difference between Blueberry Cultivar ‘STAR’ by Planted Ground and Pot

    Gyung Deok Han1, Dae Ho Jung2, Seong Heo3,*, Yong Suk Chung1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2583-2590, 2022, DOI:10.32604/phyton.2022.022866 - 12 July 2022

    Abstract In the smart farm, we can control every detail for production. Collecting every factor that affects the crop’s final yield is necessary to optimize its efficiency. The SPAD values were observed in the ‘Star’ cultivar blueberry (Vaccinium darrowii) three times a day and at three different plant heights. The pattern of SPAD value change was different by the planting position. Ground planted blueberry (V. darrowii) represented a stable SPAD value during the day and at the different heights. However, the SPAD value was increased by time in pot-planted blueberry (V. darrowii). Also, the SPAD value of pot-planted blueberry More >

  • Open Access

    ARTICLE

    Transcriptome Analysis via RNA Sequencing Reveals the Molecular Mechanisms Underlying the Hedera helix Response to High Temperature

    Ting Zhang1,2, Ping Li3,*, Jiali Wei3,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2403-2417, 2022, DOI:10.32604/phyton.2022.022421 - 12 July 2022

    Abstract Hedera helix is an evergreen ornamental plant that is resistant to cool but not high temperature and deserves to be further researched for improving its adaptability to heat stress. Two Hedera helix cultivars, heat-tolerant (HT) ‘Jessica’ and heat-sensitive (HS) ‘Shamrock’, were used for differences analyses of transcriptome. We detected 6179 differentially expressed genes (DEGs) and 5992 DEGs in ‘Jessica’ and ‘Shamrock’ to heat stress, respectively. Among these, 1983 upregulated DEGs and 1400 downregulated DEGs were shared between both varieties, resulting in enhancement of various pathways such as biosynthesis of secondary metabolites, glyoxylate dicarboxylate metabolism, and protein processing… More >

  • Open Access

    ARTICLE

    Selection and Verification of Reference Genes for qRT-PCR Analysis in Iris domestica under Drought

    Qiang Ai, Cuijing Liu*, Mei Han*, Limin Yang

    Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2537-2548, 2022, DOI:10.32604/phyton.2022.021889 - 12 July 2022

    Abstract Iris domestica is a plant of the Iridaceae family and is drought-tolerant, but its drought-resistance mechanism is not yet clear. Analysing the gene expression changes of I. domestica by qRT-PCR is an important mean to understand its drought resistance characteristics. Nevertheless, a lack of reference genes greatly hinders investigation and research on the adaptation of I. domestica to drought at the molecular and genetic levels. In this study, we assessed the expression stability of 11 candidate gene in I. domestica under drought stress conditions and different tissues using geNorm, NormFinder, BestKeeper and RefFinder tools. The results showed that EF1β was More >

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