Hans Christian Correa-Aguado^1,3, Gloria Viviana Cerrillo-Rojas¹, Alejandro Rocha-Uribe², Ruth Elena Soria-Guerra², José Francisco Morales-Domínguez^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 515-531, 2021, DOI:10.32604/phyton.2021.013619

Abstract The need for renewable energy sources makes microalgae an essential feedstock for biofuels production. The molecular aspects and the response to nitrogen (N)-limited conditions with a phytohormone stimulus in microalgae have been slightly explored. In this work, Scenedesmus obliquus was used as a study model to analyze the effect of benzyl amino purine (BAP) and gibberellic acid (GA) coupled to nitrogen limitation on cell growth, biomass and fatty acids. The selected 10^-5 M BAP increased the biomass by 1.44-fold, and 10^-6 M GA by 1.35-fold. The total lipids also increased by 2.8 and 1.11-fold, respectively. The 10^-5 M BAP and… More >

Paul Chege^1,2, Erzsébet Kiss², Csaba Lantos¹, Andrea Palágyi¹, János Pauk^1,2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 475-487, 2021, DOI:10.32604/phyton.2021.013557

Abstract Sorghum [Sorghum bicolor (L.) Moench] can benefit from accelerated breeding and release of improved varieties through doubled haploid technology. The technology has been used in speeding up the breeding of other major cereals such as wheat, maize and rice, for which generally widely applied optimised protocols exist. A reproducible protocol for the crop, that can overcome genotype dependency and other species-specific challenges such as phenolic exudation is however lacking. This study aimed at sorghum doubled haploids production thereby contributing to the development of an improved protocol. From the 28 hybrid genotypes, both F₁ registered- and experimental hybrids involved, this study… More >

Zhenzhong Jiang¹, Peng Jiao¹, Zhuo Qi¹, He Zhao², Jing Qu², Siyan Liu¹, Dan Yao¹, Piwu Wang², Shuyan Guan^1,*, Yiyong Ma^2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 449-459, 2021, DOI:10.32604/phyton.2021.013247

Abstract The concept of gene-function-genetic trait was introduced to explore the effects of early flowering on the growth and development of maize at the jointing stage and to obtain early flowering mutants using ethyl methanesulfonate mutagenesis. First, we studied gene expression, phytohormones, and lignin content to explore the physiological peculiarities of the early flowering mutant. Then we analyzed the genetic features of the mutants during the jointing stage by measuring physiological and biochemical indices of drought tolerance. The results showed that the photosynthetic rate of the mutant was significantly higher than that of the control and the rate of accumulation of… More >

Mercedes Georgina Ramírez-Aragón¹, Victoria Jared Borroel-García¹, Ramón Valenzuela-Soto¹, Ricardo David Valdez-Cepeda², Francisco Javier Wong-Corral³, José Luis García-Hernández^4,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 533-541, 2021, DOI:10.32604/phyton.2021.013166

Abstract The extraction of bioactive compounds has become one of the most interesting areas of modern chemistry. For therapeutic reasons, it´s important to obtain antimicrobial agents from natural origin. The objective of the present study was to determine the inhibitory effect of ethanolic extract of basil (Ocimum basilicum L. var. Red Rubin) subjected to different concentrations of potassium (K⁺) on the activity of three bacterial strains that are pathogens in humans. Susceptibility was evaluated by inhibition surface and these results were compared to two antibiotics: Gentamicin (GE) and Ciprofloxacin (CPF) for their efficacy against each bacterial strain. Analyzed variables presented significant… More >

Bin Du¹, Qixia Wu¹, Shuochen Jiang¹, Dingyue Zhang¹, Yue Qiao¹, Yimei Xie², Jianlong Xu^3,*, Jianqiang Zhu^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 437-447, 2021, DOI:10.32604/phyton.2021.012903

Abstract The improvement of grain quality in aromatic rice is very important for farmer to increase their income. Present study was conducted with a two-year field experiment and three aromatic rice cultivars in order to study the effects of exogenous α-ketoglutaric acid on yield formation, grain quality characters and the biosynthesis of 2-acetyl-1-pyrroline (2-AP, key component of aromatic rice’s fragrance) in aromatic rice. At heading stage, 0.50 mmol L^–1 (T1) and 1.00 mmol L^–1 (T2) α-ketoglutaric acid solutions were overhead sprinkle to aromatic rice plants, respectively while the treatment which was overhead sprinkled with distilled water was set as control (CK).… More >

Tianyuan Li^1,#, Saif Ullah^1,#, He Liang¹, Izhar Ali¹, Quan Zhao¹, Anas Iqbal¹, Shanqing Wei¹, Tariq Shah², Yuqiong Luo¹, Ligeng Jiang^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 223-243, 2021, DOI:10.32604/phyton.2020.012065

Abstract Readily available chemical fertilizers have resulted in a decline in the use of organic manure (e.g., green manures), a traditionally sustainable source of nutrients. Based on this, we applied urea at the rate of 270 kg ha⁻¹ with and without green manure in order to assess nitrogen (N) productivity in a double rice cropping system in 2017. In particular, treatment combinations were as follows: winter fallow rice-rice (WF-R-R), milk vetch rice-rice (MV-R-R), oil-seed rape rice-rice (R-R-R) and potato crop rice-rice (P-R-R). Results revealed that green manure significantly (p ≤ 0.05) improved the soil chemical properties and net soil organic carbon… More >

Pengtao Li^1,2,#,*, Quanwei Lu^1,#, Xianghui Xiao³, Rui Yang³, Xixi Duan¹

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 129-144, 2021, DOI:10.32604/phyton.2021.012928

Abstract Fiber length is a critical trait that principally determines cotton spinning quality, while Upland cotton as the most widely cultivated Gossypium species around the world subjects to the relatively ordinary fiber performance. Chromosome segment substitution lines (CSSLs) have been introduced in cotton breeding to take full advantages of superior fiber quality and high yield from Sea Island and Upland cotton, respectively, which serve as ideal materials for elucidating the genetic mechanism of complex quantitative traits. Here, three CSSLs derived from CCRI45 (G. hirsutum) × Hai1 (G. barbadense), two superior (MBI7561 and MBI7747) and one (MBI7285) with ordinary fiber-quality, were subjected… More >

Taoze Sun, Wei Tan, Yujie Yang, Hongna Mu^*

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 171-178, 2021, DOI:10.32604/phyton.2020.013668

Abstract Pinus elliottii is an exotic afforestation pine extensively distributed in southern parts of China. In order to understand whether endophytic fungi can affect seedling growth of P. elliottii, Piriformospora indica (Pi), Funnelifcrmis mosseae (Fm), and Diversispora tortuosa (Dt) were inoculated respectively, and the non-inoculated group was set as control. The growth indexes, the contents of soluble sugar and soluble protein, and plant endogenous hormone levels in the leaves of P. elliottii, were analyzed. The results showed that Fm, Dt and Pi colonized the P. elliottii roots to form mycorrhizal structure and chlamydospores arranged in beads respectively. Three fungal inoculants exhibited… More >

Siyi Fu^1,#, Tao Yun^2,#, Dexuan Ma¹, Bingsong Zheng¹, Dean Jiang³, Yi He^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 99-110, 2021, DOI:10.32604/phyton.2021.013262

Abstract The chloroplast NAD(P)H dehydrogenase (NDH) complex, as one of the most important photosynthesis protein complexes in thylakoid membrane, is involved in photosystem I (PSI) cyclic electron transport (CEF). Under abiotic environmental stress, the photosynthetic apparatus is susceptible to the damage caused by the strong light illumination. However, the enhancement of NDHdependent CEF could facilitate the alleviation of the damage to the photosynthetic apparatus. The NdhB subunit encoded by chloroplast genome is one of most important subunits of NDH complex and consists of 510 amino acids. Here, according to cloning ndhB from Melrose (cultivated soybean), ACC547 (wild salt-tolerant soybean), S113-6 and… More >

Xiubing Gao^1,3, Jiejie Lv², Can Guo¹, Anlong Hu³, Xiaomao Wu³, Zengping Li^4,*

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 179-192, 2021, DOI:10.32604/phyton.2021.012968

Abstract Hevea brasiliensis is one of the important economic trees with a great economic value for natural rubber production. Symbiosis between roots of H. brasiliensis and arbuscular mycorrhizal fungi (AMF) is widely recognized, and can provide a range of benefits for both of them. Hainan Island harbors is one of the largest plantations of H. brasiliensis in China, whereas the information regarding the diversity of AMF in the rhizosphere of H. brasiliensis on this island is scarce. The diversity of AMF species in the rhizosphere of rubber tree plantations in Hainan was investigated in this study. A total of 72 soil… More >