LUCIANA MAZZEI^1,2, MARÍA BELÉN RUIZ-ROSO³, NATALIA DE LAS HERAS³, SANDRA BALLESTEROS³, CAROLINA TORRESPALAZZOLO⁴, LEÓN FERDER⁵, ALEJANDRA BEATRIZ CAMARGO⁴, WALTER MANUCHA^1,2,*

BIOCELL, Vol.44, No.4, pp. 671-681, 2020, DOI:10.32604/biocell.2020.014175 - 24 December 2020

Abstract The ancestral cultures have described many therapeutic properties of garlic; therefore, it is of central interest to elucidate the molecular basis explaining this millenary empirical knowledge. Indeed, it has been demonstrated a neuroprotective effect of allicin–a phytochemical present in garlic- linked to oxidative-inflammatory modulation. Allicin improved neuronal injury by heat shock protein 70 (Hsp70) and inducible nitric oxide synthase (iNOS) regulation. Also, allicin exerts renal protection involving a possible angiotensin type 1 receptor (AT1) interaction. In connection, AT1 overexpression has been recognized as a central deleterious factor in many brain diseases. However, there are no… More >

WEI LIU^1,#, ZI WANG^1,2,#, JING LENG¹, HENG WEI¹, SHEN REN^1,2, XIAOJIE GONG³, CHEN CHEN⁴, YINGPING WANG^1,2, RUI ZHANG^1,2,*, WEI LI^1,2,*

BIOCELL, Vol.44, No.4, pp. 655-669, 2020, DOI:10.32604/biocell.2020.013202 - 24 December 2020

Abstract Heat stress (HS) reaction can lead to serious physiological dysfunction associated with cardiovascular and various organ diseases. Ginsenoside Rg3 (G-Rg3) is a representative component of ginseng rare saponin and can protect against multiple organs, also used as functional food to adjust the balance of the human body, but the therapeutic effect and molecular mechanism of G-Rg3 on male diseases under HS are underexplored. The aim of the present study, G-Rg3 was prepared through the efficient conversion of ginsenoside Rd and investigate the contribution of G-Rg3 to testicular injury induced exposure to HS. All mice were… More >

LIHUA CAO¹, MINGSAN MIAO^1,*, YAN ZHENG², HUIYAUN HUANG², RUI ZHANG², DANDAN LIU²

BIOCELL, Vol.44, No.4, pp. 649-653, 2020, DOI:10.32604/biocell.2020.05093 - 24 December 2020

Abstract To investigate the effects of Ginkgo paste for external use on tinea corporis in a guinea pig model. The guinea pig tinea corporis model were induced by infection with Trichophyton mentagrophytes strains. And then, high and low doses of Ginkgo water-paste and alcohol-paste were administrated to the animals. The symptom, tinea corporis skin lesions and histopathological aspects of guinea pig were analyzed. High and low doses of Ginkgo alcohol-paste and Ginkgo water-paste could significantly reduced the tinea corporis symptom (P < 0.01), increased negative rate of strain culture (P < 0.01), and improved pathological changes of tinea More >

FANGHAO SUN¹, WENCHAO ZHAO¹, LIANSHENG ZHANG², HONGGUI MA¹, JIAN ZHOU¹, YOUGAN CHEN¹, JIANQUAN HOU^1,*

BIOCELL, Vol.44, No.4, pp. 639-647, 2020, DOI:10.32604/biocell.2020.013053 - 24 December 2020

Abstract Recent studies suggested that LIM and SH3 protein 1 (LASP-1) is a promising therapeutic target for renal cell cancer (RCC). This study aimed to explore the role of LASP-1 in RCC. For this purpose, LASP-1 expression in RCC tissues was analyzed by immunohistochemistry and Western blot analysis. Cell proliferation, migration, invasion, and gene expression were detected by CCK-8 assay, Transwell assay, and Western blot analysis. The results showed that LASP-1 was highly expressed in RCC, and its expression level,t was positively correlated with lymph node metastasis and tumor, nodes, and metastases (TNM) stage. The knockdown More >

ZIMING WEI^1,2, YARONG WANG³, MINGYUAN DU^1,4, MINJIE LI², HONG HE^1,4,*

BIOCELL, Vol.44, No.4, pp. 631-638, 2020, DOI:10.32604/biocell.2020.010596 - 24 December 2020

Abstract Periodontitis, as a chronic inflammatory disease, remains unsolved, and the pathogenesis of this disease has not been fully elucidated. In this study, the effect of miR-4262 was investigated in tumor necrosis factor-α (TNF-α) induced human periodontal stem cells (hPDLSCs) for the first time. The gene expression involved in this study was determined using polymerase chain reaction (PCR), the expressions of relevant proteins were determined by western blot analysis, and the levels of IL-1β, IL-6, and MCP-1 were estimated by enzyme-linked immunosorbent assay (ELISA) assay. The luciferase reporter assay was performed for verification of the target More >

JIYU WANG^#, HUIPING WANG^#, YING PAN, QIANSHAN TAO, ZHIMIN ZHAI^*

BIOCELL, Vol.44, No.4, pp. 607-611, 2020, DOI:10.32604/biocell.2020.010947 - 24 December 2020

Abstract Myelodysplastic syndromes (MDS) are highly heterogeneous myeloid neoplasms, and a large number of patients are difficult to diagnose and classify by blood and bone marrow examination. As a surface marker of granulocyte, studies have shown CD10 can be used to define the degree of granulocyte maturation in MDS patients. However, whether it can be used for differential diagnosis of MDS and other hematological diseases remains inconclusive. To explore the value of CD10 for differential diagnosis of MDS, 60 newly diagnosed MDS, 20 aplastic anemia (AA) patients, and 35 iron-deficient anemia (IDA) patients were selected for… More >

XIANLIANG HOU^1,2,3,#, DONGE TANG^1,#, FENGPING ZHENG^1,#, MINGLIN OU³, YONG XU¹, HUIXUAN XU¹, XIAOPING HONG⁴, XINZHOU ZHANG¹, WEIER DAI⁵, DONGZHOU LIU^4,*, YONG DAI^1,*

BIOCELL, Vol.44, No.4, pp. 559-582, 2020, DOI:10.32604/biocell.2020.011022 - 24 December 2020

Abstract Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by abnormal cellular and humoral immune responses and excessive autoantibody production. The precise pathologic mechanism of SLE remains elusive. The advent of single-cell RNA sequencing (scRNA-seq) enables unbiased analysis of the molecular differences of cell populations at the single-cell level. We used scRNA-seq to profile the transcriptomes of peripheral blood mononuclear cells from an SLE patient compared with a healthy control (HC). A total of 16,021 cells were analyzed and partitioned into 12 distinct clusters. The marker genes of each cluster and the four major… More >

LINNA TAN^1,2, YUYONG TAN^1,2, DELIANG LIU^1,2,*

BIOCELL, Vol.44, No.4, pp. 535-543, 2020, DOI:10.32604/biocell.2020.09171 - 24 December 2020

Abstract ULK1 (unc-51 like autophagy activating kinase 1), a mammalian serine/threonine kinase, is a key component of autophagy initiation complex and helps to induce all types of autophagy. Canonical autophagy is a process in which, through the interactions of a series of autophagy-related proteins, damaged organelles or misfolded proteins are engulfed by autophagosomes and then merged with lysosomes to be degraded. Thus, canonical autophagy is an important constituent part of the cellular “quality control.” Besides, accumulating evidence indicates that ULK1 exerts autophagy-independent effects in a cell-specific manner. For example, ULK1 facilitates neurite elongation through the regulation More >

LU GAN, XIAOSHU DENG, YAN LIU, ANCAI LUO, JIAO CHEN, JING XIANG, ZHENGWU ZHAO^*

BIOCELL, Vol.44, No.3, pp. 451-459, 2020, DOI:10.32604/biocell.2020.08007 - 22 September 2020

Abstract The present study focused on the segregation of the percentage of grains with chalkiness (PGWC), using Huanghuazhan as the female parent and CS197 as the male parent to construct the hybrid rice F₂ population. Molecular markers were used for genotype analysis among the extremely low and extremely high PGWC individuals from the F₂ population. The results revealed that the genotypes of 10 extremely low PGWC individuals were 80.00% and 3.33%, which is identical to Huanghuazhan and CS197, respectively. The heterozygotes accounted for 16.67%. On the contrary, the genotypes of 10 extremely high PGWC individuals were 37.78%… More >

HELIN FENG^1,2, XIAOCHONG ZHANG³, QIANQIAN ZHANG⁴, ZE LI⁵, LILI ZHAO^1,6,*

BIOCELL, Vol.44, No.3, pp. 443-450, 2020, DOI:10.32604/biocell.2020.010705 - 22 September 2020

Abstract Osteosarcoma (OS) is a primary high-grade malignant bone neoplasm, and the prognosis of OS remains poor due to early metastasis. Leptin plays an essential role in tumorigenesis, but the role of leptin in the development of OS is still not fully understood. In this study, we used a human osteosarcoma MG-63 cell line as an experimental model. MG-63 cells were treated with leptin, and cell proliferation, apoptosis, adhesion, invasion, and gene expression, were evaluated. The results showed that leptin promoted proliferation, decreased adhesion, suppressed apoptosis, and promoted invasion, of MG-63 cells. Moreover, the expression of More >