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  • Open Access

    ARTICLE

    Ectopic Overexpression of EuCHIT30.7 Improves Nicotiana tabacum Resistance to Powdery Mildew

    Lanjun Li1, Degang Zhao1,2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3043-3061, 2023, DOI:10.32604/phyton.2023.031175

    Abstract Various strains of powdery mildew (PM), a notorious plant fungal disease, are prevalent and pose a significant threat to plant health. To control PM, transgenic technology can be used to cultivate more resistant plant varieties. In the present study, we utilized the rapid amplification of cDNA ends (RACE) technique to clone the full-length cDNA sequence of the EuCHIT30.7 gene to explore plant genes with disease resistance functions. Bioinformatics analysis revealed that this gene belongs to the GH18 family and is classified as a class III chitinase. The EuCHIT30.7 gene is expressed throughout the Eucommia ulmoides plant, with the most abundant… More >

  • Open Access

    ARTICLE

    Comparative transcriptome analysis provides insights into the molecular mechanism of the anti-nematode role of Arachis hypogaea (Fabales: Fabaceae) against Meloidogyne incognita (Tylenchida: Heteroderidae)

    XUEJIN YANG1,2,#, YUANYUAN ZHOU1,#, XINYI PENG1, XIAOHONG FU1, JIANQING MA1, JIANFENG LIU1,*, DANDAN CAO1,2,*

    BIOCELL, Vol.47, No.9, pp. 2101-2113, 2023, DOI:10.32604/biocell.2023.029581

    Abstract Background: Plant root-knot nematode (RKN) disease is a serious threat to agricultural production across the world. Meloidogyne incognita is the most prominent pathogen to the vegetables and cash crops cultivated. Arachis hypogaea can effectively inhibit M. incognita, but the underlying defense mechanism is still unclear. Methods: In our study, the chemotaxis and infestation of the second-stage juveniles (J2s) of M. incognita to A. hypogaea root tips were observed by the Pluronic F-127 system and stained with sodium hypochlorite acid fuchsin, respectively. The transcriptome data of A. hypogaea roots with non-infected or infected by J2s were analyzed. Results: The J2s could… More >

  • Open Access

    ARTICLE

    Transcriptome and Metabolome Revealed the Mechanism of NtBRL3 Overexpression Tobacco (Nicotiana tabacum L. K326) in Response to Drought Stress

    Jing Yang, Tianxiunan Pu, Ke Wan, Linqi Wang, Yuanshuai Shi, Xu Luo, Jie Tan, Dongmei Wang, Yang Liu*

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2555-2576, 2023, DOI:10.32604/phyton.2023.030301

    Abstract Drought has severely affected the yield and quality of commercial crops. The BRI1 family plays an important role in plant response to drought stress, and BRL3 gene plays an important role in the study of drought in Arabidopsis thaliana. In this study, NtBRL3 was constructed as a vector and genetically transformed to obtain ‘N. Tobacco K326’ overexpression of NtBRL3. The enzyme activities of transgenic tobacco and wild-type tobacco were measured and transcriptome and metabolome analyses were performed. The results showed that the antioxidant enzymes of transgenic tobacco were more active under drought conditions, and 85 significantly differentially metabolites and 106… More >

  • Open Access

    ARTICLE

    Multidimensional Quality Evaluation of Graduate Thesis: Based on the Probabilistic Linguistic MABAC Method

    Yuyan Luo1,2, Xiaoxu Zhang1,*, Tao Tong1, Yong Qin3,*, Zheng Yang1

    CMES-Computer Modeling in Engineering & Sciences, Vol.137, No.2, pp. 2049-2076, 2023, DOI:10.32604/cmes.2023.025413

    Abstract Graduate education is the main way to train high-level innovative talents, the basic layout to cope with the global talent competition, and the important cornerstone for implementing the innovation-driven development strategy and building an innovation-driven country. Therefore, graduate education is of great remarkably to the development of national education. As an important manifestation of graduate education, the quality of a graduate thesis should receive more attention. It is conducive to promoting the quality of graduates by supervising and examining the quality of the graduate thesis. For this purpose, this work is based on text mining, expert interviews, and questionnaire surveys… More >

  • Open Access

    ARTICLE

    Ether-IoT: A Realtime Lightweight and Scalable Blockchain-Enabled Cache Algorithm for IoT Access Control

    Hafiz Adnan Hussain*, Zulkefli Mansor, Zarina Shukur, Uzma Jafar

    CMC-Computers, Materials & Continua, Vol.75, No.2, pp. 3797-3815, 2023, DOI:10.32604/cmc.2023.034671

    Abstract Several unique characteristics of Internet of Things (IoT) devices, such as distributed deployment and limited storage, make it challenging for standard centralized access control systems to enable access control in today’s large-scale IoT ecosystem. To solve these challenges, this study presents an IoT access control system called Ether-IoT based on the Ethereum Blockchain (BC) infrastructure with Attribute-Based Access Control (ABAC). Access Contract (AC), Cache Contract (CC), Device Contract (DC), and Policy Contract (PC) are the four central smart contracts (SCs) that are included in the proposed system. CC offers a way to save user characteristics in a local cache system… More >

  • Open Access

    ARTICLE

    Genome-Wide Identification and Expression Analysis of the Phytocyanin Gene Family in Nicotiana tabacum

    Peiling Wang1,#, Xiaohong Xu2,#, Yong Li2, Hecui Zhang1, Xuejie Zhang3, Siru Zhou1, Yimei Liu1, Yunyan Feng1, Tonghong Zuo1, Liquan Zhu1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1469-1492, 2023, DOI:10.32604/phyton.2023.027133

    Abstract Phytocyanin (PC) is a class of plant-specific blue copper proteins involved in electron transport, plant growth, development, and stress resistance. However, PC proteins have not been systematically evaluated in tobacco plants. We determined the whole-genome sequences of the PC family in the tobacco cultivar ‘K326.’ The transcriptome data were used to analyze the expression of the NtPC family at different development stages and tissue-specific genes. Real-time fluorescence quantitative analysis was used to analyze the expression of the NtPC gene family under low temperature and methyl jasmonate stress. The tobacco NtPC family contained 110 members and was divided into four subfamilies:… More >

  • Open Access

    REVIEW

    Aluminum Toxicity: A Case Study on Tobacco (Nicotiana tabacum L.)

    Munir Ozturk1,*, Mert Metin2, Volkan Altay3, Tomonori Kawano2, Alvina Gul4, Bengu Turkyilmaz Unal5,*, Dilek Unal6, Rouf Ahmad Bhat7, Moonisa Aslam Dervash8, Kristina Toderich9, Esra Koc10, Pedro Garcia Caparros11, Andleeb Shahzadi12

    Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 165-192, 2023, DOI:10.32604/phyton.2022.022038

    Abstract Aluminum is an abundant metal in the earth’s crust that turns out to be toxic in acidic environments. Many plants are affected by the presence of aluminum at the whole plant level, at the organ level, and at the cellular level. Tobacco as a cash crop (Nicotiana tabacum L.) is a widely cultivated plant worldwide and is also a good model organism for research. Although there are many articles on Al-phytotoxicity in the literature, reviews on a single species that are economically and scientifically important are limited. In this article, we not only provide the biology associated with tobacco Al-toxicity,… More >

  • Open Access

    ARTICLE

    Research on ABAC Access Control Based on Big Data Platform

    Kun Yang1, Xuanxu Jin2, Xingyu Zeng1,*

    Journal of Cyber Security, Vol.3, No.4, pp. 187-199, 2021, DOI:10.32604/jcs.2021.026735

    Abstract In the environment of big data, the traditional access control lacks effective and flexible access mechanism. Based on attribute access control, this paper proposes a HBMC-ABAC big data access control framework. It solves the problems of difficult authority change, complex management, over-authorization and lack of authorization in big data environment. At the same time, binary mapping codes are proposed to solve the problem of low efficiency of policy retrieval in traditional ABAC. Through experimental analysis, the results show that our proposed HBMC-ABAC model can meet the current large and complex environment of big data. More >

  • Open Access

    ARTICLE

    A New Decision-Making Model Based on Plithogenic Set for Supplier Selection

    Mohamed Abdel-Basset1,*, Rehab Mohamed1, Florentin Smarandache2, Mohamed Elhoseny3

    CMC-Computers, Materials & Continua, Vol.66, No.3, pp. 2751-2769, 2021, DOI:10.32604/cmc.2021.013092

    Abstract Supplier selection is a common and relevant phase to initialize the supply chain processes and ensure its sustainability. The choice of supplier is a multi-criteria decision making (MCDM) to obtain the optimal decision based on a group of criteria. The health care sector faces several types of problems, and one of the most important is selecting an appropriate supplier that fits the desired performance level. The development of service/product quality in health care facilities in a country will improve the quality of the life of its population. This paper proposes an integrated multi-attribute border approximation area comparison (MABAC) based on… More >

  • Open Access

    ARTICLE

    Germinating seeds of the mung bean, Vigna radiata (Fabaceae), as a model for the preliminary evaluation of cytotoxic effects of drugs

    VIJAY L. KUMAR*, ABHISHEK SINGHAL

    BIOCELL, Vol.33, No.1, pp. 19-24, 2009, DOI:10.32604/biocell.2009.33.019

    Abstract Cytotoxic properties of plant extracts and drugs being developed for cancer treatment are usually evaluated by a variety of in vivo and in vitro tests carried out in animal or plant based models. In the present study we have evaluated the possibility of using the germinating mung beans (Vigna radiata), for rapid and inexpensive screening of drugs exhibiting cytotoxic properties. Mung beans were allowed to germinate either in tap water or in different drug solutions, and parameters like percent germination, increase in radicle length, change in seedling weight and mitotic index of apical root meristems were determined at two time… More >

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