Nancy Abril Martínez-López¹, Betsy Anaid Peña-Ocaña², Rodolfo García-Contreras³, Toshinari Maeda⁴, Reiner Rincón-Rosales¹, Federico Antonio Gutiérrez-Miceli¹, Víctor Manuel Ruíz-Valdiviezo^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2729-2743, 2025, DOI:10.32604/phyton.2025.068176 - 30 September 2025

Abstract Species of the genus Paenibacillus, especially those from extreme environments that have been reported, are known for producing bioactive compounds with agricultural and biotechnological applications. In this study, we investigated the genomic and biochemical potential of Paenibacillus sp. VCA1 strain isolated from a thermophilic environment. Taxonomic identification was performed using whole genome similarity analysis, TETRA four-nucleotide frequency of occurrence analysis, ANI average nucleotide identity analysis, and gene distance analysis using digital DNA-DNA hybridization (dDDH). Functional analysis of the strain VCA1 was performed by detecting genes, enzymes, and genome subsystems involved in biocontrol and plant growth promotion,… More >

Gilciany Ribeiro Soares¹, Jiovana Kamila Vilas Boas¹, Fábio Steiner^1,2, Jorge González Aguilera^2,*, Alan Mario Zuffo³, José Vitor Marçal do Prado², Wellingthon da Silva Guimarães Júnnyor², Leandris Argentel- Martínez⁴, Luis Morales-Aranibar⁵

Phyton-International Journal of Experimental Botany, Vol.94, No.8, pp. 2347-2363, 2025, DOI:10.32604/phyton.2025.066264 - 29 August 2025

Abstract Corn (Zea mays L.) is a very sensitive crop to phosphorus (P) deficiency during the early development phase, which may be a limiting factor for the sustainable production of this crop in P-deficient tropical soils. However, scientific evidence indicates that inoculation with phosphate-solubilizing bacteria can improve the development, uptake, and P-use efficiency of corn plants. In the present study, two contrasting corn hybrids were investigated for their responsiveness to multiple inoculations of Bacillus subtilis, B. megaterium, B. velezencis, and Pseudomonas fluorescens and application of phosphate fertilizer rates in the sandy soil of the Brazilian Cerrado. Plants from stable (DKB 360… More >

Nu Ri Song^1,#, Seo Yeon Shin^1,#, Ki Min Kim¹, Sa Rang Choi¹, Doo Sang Park², Sun Oh Kim³, Dai Hyun Jung⁴, Kyung Mok Park^1,*

BIOCELL, Vol.49, No.7, pp. 1291-1310, 2025, DOI:10.32604/biocell.2025.065467 - 25 July 2025

Abstract Objectives: Limosilactobacillus reuteri is a beneficial Lactobacillus widely used in foods and supplements to promote overall health. Some studies also suggest it supports skin health and prevents allergies and cardiovascular disease. However, research on its skin-protective effects against photoaging has not been conducted. This study evaluated the potential of culture broths from three L. reuteri strains (DS0333, DS0384, and DS0385) to inhibit skin photoaging. Methods: To assess their anti-photoaging potential, the culture broths were examined for antioxidant capacity, melanin inhibition, and collagen synthesis promotion. Radical scavenging activity was tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2^′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.… More > Graphic Abstract

LUCIANA SABOYA^1,2, KEINI BUOSI¹, TIAGO SILVA², ELAINE CANDIDO¹, JOSIANE MORARI³, LICIO A. VELLOSO³, SHAHROKH F. SHARIAT^4,5,6,7, MARCUS V. SADI², LEONARDO O. REIS^1,8,9,*

Oncology Research, Vol.33, No.6, pp. 1495-1503, 2025, DOI:10.32604/or.2025.061812 - 29 May 2025

Abstract Objective: To determine the safety and the role of modulating cytokines and proteases in the immune response to intravesical Bacillus Calmette-Guérin (BCG) when primed with systemic intradermal BCG. Methods: Phase 1 and mechanistic longitudinal, prospective, single-blind randomized study (NCT04806178). Twenty-one non-muscle invasive urothelial bladder cancer patients undergoing intravesical adjuvant BCG after transurethral resection of bladder tumor (TURBT) in a teaching hospital between September 2021 and April 2023 were randomized to 0.1 mL of intradermal BCG vaccine or placebo (0.9% saline) administered 15 days before the start of intravesical BCG therapy. Blood samples were evaluated mechanistically… More >

Francesco Triggiano^#, Giusy Diella^#, Mara Lorusso, Paolo Veneziani^*, Marilena D’Ambrosio, Daniela Nesta, Luigi Santacroce^*

BIOCELL, Vol.49, No.2, pp. 199-220, 2025, DOI:10.32604/biocell.2025.059322 - 28 February 2025

Abstract The composition of the vaginal microbiota (VMB) influences the health of the female reproductive tract. Several studies have shown how the absence of lactobacilli causes an imbalance in the vaginal microbial community, favoring the development of infections. The present study aims to evaluate the relationship between the VMB and human papillomavirus (HPV) infection to clarify the role of the vaginal microbiota in the persistence and clearance of HPV. Many researchers have provided the scientific community with information on the composition of the microbiota and how it may also influence HPV infection and the development of… More >

BELARDINILLI FRANCESCA^1,#, MICHELA DE MEO^1,#, FRANCESCO DEL GIUDICE², CARLO MARIA SCORNAJENGHI², PAOLA GAZZANIGA¹, ETTORE DE BERARDINIS², LUCA MARINO³, FABIO MASSIMO MAGLIOCCA⁴, BENJAMIN INBEH CHUNG⁵, JAN ŁASZKIEWICZ⁶, VALENTINA MAGRI⁴, GIUSEPPE GIANNINI^1,7, CHIARA NICOLAZZO^1,*

Oncology Research, Vol.33, No.3, pp. 723-731, 2025, DOI:10.32604/or.2024.056282 - 28 February 2025

Abstract Objectives: Intravesical Bacillus Calmette-Guérin (BCG) therapy is a gold standard for patients with high-risk non-muscle invasive bladder cancer (NMIBC). Although a long-lasting therapeutic response is observed in most patients, BCG failure occurs in 30%–50% of patients and a progression to muscle-invasive disease is found in 10%–15%. Therefore, predicting high-risk patients who might not benefit from BCG treatment is critical. The purpose of this study was to identify, whether the presence of specific oncogenic mutations might be indicative of BCG treatment response. Methods: Nineteen high-grade NMIBC patients who received intravesical BCG were retrospectively enrolled and divided… More > Graphic Abstract

Bouazza Chami^1,2, Nabil Touzout², Souad Guemouri-Athmani¹, Djamel Baali-Cherif³, Adil Mihoub⁴, Jakub Černý^5,*, Muhammad Farhan Saeed⁶, Aftab Jamal^7,*, Hayi Mohamed Yassine⁸, Yaser Hassan Dewir⁹, Aurelio Scavo¹⁰

Phyton-International Journal of Experimental Botany, Vol.93, No.12, pp. 3313-3329, 2024, DOI:10.32604/phyton.2024.059865 - 31 December 2024

Abstract Intensive agricultural practices have undeniably reduced soil fertility and crop productivity. Furthermore, alkaline calcareous soils represent a significant challenge for agricultural production, particularly durum wheat, which is vital for ensuring food security. It is therefore essential to explore new cereal management strategies to maintain food production and promote crop sustainability. The application of soil microorganisms, particularly plant growth–promoting rhizobacteria (PGPR), as inoculants to enhance crop production is a growing area of interest. This study investigates the effects of the rhizobacteria Paenibacillus polymyxa SGH1 and SGK2, applied both individually and in combination, on the growth and… More >

GUSTAVO MARTÍN VILLOLDO¹, MARÍA TERESA POMBO², MARIANA ARIS³, JOAQUÍN CHEMI¹, PABLO MANDÓ³, SUPRIYA NAGARAJU⁴, JUAN CAMEAN¹, ADRIÁN BURIONI¹, DEBORAH EGEA¹, MORA AMAT⁵, JOSÉ LEÓN MELLADO³, JOSÉ MORDOH³, ALBERTO VILLARONGA¹, MARÍA MARCELA BARRIO^3,*

Oncology Research, Vol.31, No.2, pp. 207-220, 2023, DOI:10.32604/or.2023.028163 - 10 April 2023

Abstract Intravesical Bacillus Calmette Guerin (BCG) is the gold standard therapy for intermediate/high-risk nonmuscle invasive bladder cancer (NMIBC). However, the response rate is ~60%, and 50% of non-responders will progress to muscle-invasive disease. BCG induces massive local infiltration of inflammatory cells (Th1) and ultimately cytotoxic tumor elimination. We searched for predictive biomarker of BCG response by analyzing tumor-infiltrating lymphocyte (TIL) polarization in the tumor microenvironment (TME) in pre-treatment biopsies. Pre-treatment biopsies from patients with NMIBC who received adequate intravesical instillation of BCG (n = 32) were evaluated retrospectively by immunohistochemistry. TME polarization was assessed by quantifying… More > Graphic Abstract

Touseef Hussain^1,*, Abrar A. Khan¹, Heba I. Mohamed^2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 783-799, 2023, DOI:10.32604/phyton.2022.026045 - 29 November 2022

Abstract Fusarium dry rot is considered to be the most critical soilborne and postharvest disease that damages potato tubers worldwide when they are stored for a long time. This study was performed to demonstrate the effect of crude extract, culture filtrate, and cell suspension obtained from the bacterium Bacillus subtilis HussainT-AMU, on the net house and field. From oil-contaminated soil, through the serial dilution method, biosurfactant bacteria were isolated on nutrient agar medium. To isolate and screen the prospective biosurfactant strain, various biosurfactant screening methods were used. Standard protocols were carried out for morphological, molecular, and chemical… More >

Chengyang Li^1,2,#, Lianjun Wang^1,#, Shasha Chai^1,#, Yinghua Xu^1,2, Chong Wang^1,2, Yi Liu^1,2, Jian Lei¹, Xiaojie Jin¹, Xianliang Cheng¹, Yuanyuan Yang¹, Xinsun Yang^1,*, Wenying Zhang^2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1779-1793, 2022, DOI:10.32604/phyton.2022.020192 - 14 April 2022

Abstract Fusarium wilt, a disease caused by Fusarium oxysporum f.sp batatas (Fob) is an important disease in sweet potato production. Using endophytic bacteria for biological control of sweet potato diseases is one of the important ways. A Bacillus subtilis with antagonistic effect on Fusarium wilt of sweet potato was isolated from soil by confrontation culture. According to the biological characteristics, 16S rDNA sequence analysis, and physiological and biochemical analysis, the Bacillus subtilis HAAS01 was named. A pot experiment was conducted for the biological control experiment of strain HAAS01, and the endogenous hormone content, antioxidant enzyme activity, soluble protein content, and related More >