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  • Open Access


    Biological Control of Root-Knot Nematode Meloidogyne incognita in Psoralea corylifolia Plant by Enhancing the Biocontrol Efficacy of Trichoderma harzianum Using Press Mud

    Yasar Nishat1, Mohammad Danish1,*, Heba I. Mohamed2,*, Hisamuddin Shaikh1, Abeer Elhakem3

    Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1757-1777, 2022, DOI:10.32604/phyton.2022.021267

    Abstract Meloidogyne incognita is a plant pathogen causing root-knot disease and loss of crop yield. The present study aimed to use Trichoderma harzianum as a biocontrol agent against plant-parasitic nematodes and used press mud, which is a solid waste by-product of sugarcane, as a biocontrol agent and biofertilizer. Therefore, the combined application of T. harzianum and press mud may enhance nematode control and plant growth. Elemental analysis of press mud using scanning electron microscopy (SEM) integrated with an Energy Dispersive X-ray (EDX) analyzer revealed the presence of different elements such as C, O, Mg, Si, P, K, Ca, Cu and Zn.… More >

  • Open Access


    Screening of Bacillus subtilis HAAS01 and Its Biocontrol Effect on Fusarium wilt in Sweet Potato

    Chengyang Li1,2,#, Lianjun Wang1,#, Shasha Chai1,#, Yinghua Xu1,2, Chong Wang1,2, Yi Liu1,2, Jian Lei1, Xiaojie Jin1, Xianliang Cheng1, Yuanyuan Yang1, Xinsun Yang1,*, Wenying Zhang2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1779-1793, 2022, DOI:10.32604/phyton.2022.020192

    Abstract Fusarium wilt, a disease caused by Fusarium oxysporum f.sp batatas (Fob) is an important disease in sweet potato production. Using endophytic bacteria for biological control of sweet potato diseases is one of the important ways. A Bacillus subtilis with antagonistic effect on Fusarium wilt of sweet potato was isolated from soil by confrontation culture. According to the biological characteristics, 16S rDNA sequence analysis, and physiological and biochemical analysis, the Bacillus subtilis HAAS01 was named. A pot experiment was conducted for the biological control experiment of strain HAAS01, and the endogenous hormone content, antioxidant enzyme activity, soluble protein content, and related… More >

  • Open Access


    In vitro Antibacterial Activity of Moringa oleifera Ethanolic Extract against Tomato Phytopathogenic Bacteria

    Roberto Arredondo-Valdés1, Francisco D. Hernández-Castillo2, Mario Rocandio-Rodríguez1, Julia C. Anguiano-Cabello3, Madai Rosas-Mejía1, Venancio Vanoye-Eligio1, Salvador Ordaz-Silva4, Imelda V. López-Sánchez4, Laura D. Carrazco-Peña4, Julio C. Chacón-Hernández1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 895-906, 2021, DOI:10.32604/phyton.2021.014301

    Abstract The tomato (Solanum lycopersicum L.) is one of the world’s most important vegetable crops. Still, phytopathogenic bacteria affect the yield and quality of tomato cultivation, like Agrobacterium tumefeciens (At), Clavibacter michiganensis subsp. michiganensis (Cmm), Pseudomonas syringae pv. tomato (Pst), Ralstonia solanacearum (Rs), and Xanthomonas axonopodis (Xa). Synthetic chemical products are used mostly on disease plant control, but overuse generates resistance to bacterial control. This study aimed to evaluate the in vitro antibacterial activity of the ethanolic extract of Moringa oleifera Lam. leaves against At, Cmm, Pst, Rs, and Xa, as well as information about this plant species’ chemical composition. Antibacterial… More >

  • Open Access


    Biology of Dittrichia viscosa, a Mediterranean ruderal plant: a review

    Parolin P, M Ion Scotta, C Bresch

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 251-262, 2014, DOI:10.32604/phyton.2014.83.251

    Abstract Dittrichia viscosa is a common plant species in the Mediterranean region which is adapted to a wide range of environmental stresses. It is an important species that can be used for phytoremediation, as bioaccumulator or bioindicator, and it has the potential for being employed in integrated pest management in the Mediterranean ecosystem due to its entomophilous character. More >

  • Open Access


    Biocontrol of pepper wilt with three Bacillus species and its effect on growth and yield

    Hernández-Castillo FD1, RH Lira-Saldivar2, G Gallegos-Morales1, M Hernández-Suárez1, S Solis-Gaona2

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 49-55, 2014, DOI:10.32604/phyton.2014.83.049

    Abstract One of the most severe phytosanitary problems that face chili pepper producers in Mexico, and in many other parts of the world, is the disease known as "secadera" or wilting, caused by diverse pathogens. These patogens are mainly controlled with synthetic pesticides, thus causing a severe ecological impact, toxicity to humans, generation of plant resistance to fungicides, and increments of production costs. Because of this, it rises the need of finding more environmentally friendly options. We evaluated rhizospheric bacteria as a possible biological control of pepper wilt. We used three bacterial strains belonging to the Bacillus genera. These strains were… More >

  • Open Access


    Biological control of chili pepper root rot (Capsicum annuum L.) by Bacillus thuringiensis

    Mojica-Marín1* V, HA Luna-Olvera2, CF Sandoval-Coronado2, B Pereyra-Alférez2, LH Morales-Ramos2, NA González-Aguilar2, CE Hernández-Luna2, OG Alvarado-Gomez3

    Phyton-International Journal of Experimental Botany, Vol.78, pp. 105-110, 2009, DOI:10.32604/phyton.2009.78.105

    Abstract Wide spreading of pepper blight on new plantations and on different production areas in Mexico, and the difficulty in controlling this disease, makes it compulsory to develop new control measures. It is expected that these measures provide new tools for controlling this disease in the context of integrated management strategies. The aim of this investigation was to evaluate and determine the antagonistic potential of 64 strains of Bacillus thuringiensis against Rhizoctonia solani, Phytophthora capsici and Fusarium oxysporum by dual culture assays. Strains of B. thuringiensis came from the International Collection of Entomopatogenic Bacillus from FCBUANL. Results showed that 16 strains… More >

  • Open Access


    Antifungal potential of Bacillus spp. strains and Larrea tridentata extract against Rhizoctonia solani on potato (Solanum tuberosum L.) crop

    Hernández-Castillo1 FD, RH Lira-Saldivar2, L Cruz-Chávez1, G Gallegos-Morales1, ME Galindo-Cepeda1, E Padrón-Corral1, M Hernández-Suárez2

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 241-252, 2008, DOI:10.32604/phyton.2008.77.241

    Abstract Potato crop requires more fungicides than any other crop in Mexico to prevent and control several diseases. More than 21,3% of the total available fungicides are required for cropping potato. High quantities of synthetic pesticides are intended to control Phytophthora infestans and Rhizoctonia solani fungi. As a result, this produces severe health problems and ecosystem disturbances. There is then an urgent need for finding new options for sustainable management of potato crop diseases. Several experiments were conducted under laboratory, greenhouse, and field conditions, to: (1) analyze the antifungal effect of bacteria strains of the genus Bacillus, and their effect on… More >

  • Open Access


    In vitro antagonism of Trichoderma harzianum on Sclerotium cepivorum Berk. and S. rolfsii Sacc., causal agents of onion rot

    Zúñiga-Mendoza E, LF Ceja-Torres

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 7-13, 2017, DOI:10.32604/phyton.2017.86.007

    Abstract In vitro tests were carried out, to evaluate the antagonist capacity of a strain of Trichoderma isolated from soil in La Cienega de Chapala, Michoacán, Mexico against Sclerotium cepivorum and Sclerotium rolfsii, casual agents of onion rot, an important disease in Mexico and the rest of the world. Percentages of radial growth inhibition (PRGI) were calculated every 24 h, until a rate of antagonism was obtained according to the Bell’s scale, as well as the percentages of inhibition of the production of sclerotia and their parasitism. The PRGI was 17.4% against S. rolfsii and 22.2% against S. cepivorum after 48… More >

  • Open Access


    Species of Trichoderma antagonistic to the root knot nematode (Meloidogyne incognita) in habanero pepper

    Herrera-Parra E1,2, J Ramos-Zapata1, J Cristóbal-Alejo3, J Tun-Suarez3, A Reyes-Ramírez3

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 7-13, 2018, DOI:10.32604/phyton.2018.87.007

    Abstract The root knot nematode Meloidogyne spp. is an important endoparasite limiting the cultivation of horticultural species and affecting the plants of at least 3000 species, including grasses, vegetables, ornamentals, fruit and forest. The aim of this study was to estimate the potential of three species of Trichoderma as antagonists of M. incognita in plants of Capsicum chinense under greenhouse conditions. A bioassay was established with plants of C. chinense previously inoculated with Trichoderma spp. and transplanted to pots with sterile substrate; the plants were subsequently inoculated with 300 infective second-stage larvae (J2) and 1000 larvae eggs of M. incognita. A… More >

  • Open Access


    Control Alternatives for Damping-Off in Tomato Seedling Production

    A. C. Michel-Aceves1, J. F. Díaz-Nájera1, R. Ariza-Flores2, M. A. Otero-Sánchez1, R. Escobar-Martínez1 and C. H. Avendaño-Arrazate3,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 325-333, 2019, DOI:10.32604/phyton.2019.06777

    Abstract In two tomato genotypes, we assessed control alternatives for damping-off with combinations of chemical fungicides and native/commercial strains of biological agents. Forty treatments consisting of 19 levels of mixing products, chemical fungicides, native strains and commercial products from biological control agents, and untreated treatment were used onto Ramsés and Toro hybrids. They were distributed on an incomplete block design in divided plots arrangement, where genotypes constitute the larger ones and the 8-repetition mixed products, the smaller ones. Putting 180 mL of fungal complexes, made of spores and mycellium Fusarium-solani (2 × 106 UFC), Rhizoctonia-solani (1 × 106 UFC), Phytophthora-capsici (1… More >

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