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  • Open Access

    ARTICLE

    Biosynthesis of raw starch degrading β-cyclodextrin glycosyltransferase by immobilized cells of Bacillus licheniformis using potato wastewater

    YASSER S. MOSTAFA1,*, SAAD A. ALAMRI1,2, SULIMAN A. ALRUMMAN1, TAREK H. TAHA3, MOHAMED HASHEM1,4, MAHMOUD MOUSTAFA1,5, LAMIAA I. FAHMY6

    BIOCELL, Vol.45, No.6, pp. 1661-1672, 2021, DOI:10.32604/biocell.2021.016193

    Abstract The study was sought to enhance the synthesis of thermal stable β-cyclodextrin glycosyltransferase (β-CGTase) using potato wastewater as a low-cost medium and assess the degree to which it is efficient for industrial production of β-cyclodextrin (β-CD) from raw potato starch. Thermophilic bacteria producing β-CGTase was isolated from Saudi Arabia and the promising strain was identified as Bacillus licheniformis using phylogenetic analysis of the 16S rRNA gene. Alginate-encapsulated cultures exhibited twice-fold of β-CGTase production more than free cells. Scanning electron microscopy (SEM) of polymeric capsules indicated the potential for a longer shelf-life, which promotes the restoration of… More >

  • Open Access

    REVIEW

    Insights into the Roles of Melatonin in Alleviating Heavy Metal Toxicity in Crop Plants

    Ling Li*,#, Xuyu Yan#

    Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1559-1572, 2021, DOI:10.32604/phyton.2021.016692

    Abstract Alleviating heavy metal pollution in farmland soil, and heavy metal toxicity in plants is the focus of global agricultural environmental research. Melatonin is a kind of indoleamine compound that wide exists in organisms; it is currently known as an endogenous free radical scavenger with the strongest antioxidant effect. As a new plant growth regulator and signaling molecule, melatonin plays an important role in plant resistance to biotic or abiotic stress. Recent studies indicate that melatonin can effectively alleviate heavy metal toxicity in crop plants, which provides a new strategy to minimize heavy metal pollution in… More >

  • Open Access

    ARTICLE

    Physiologic and Transcriptomic Insights into the High Alkali Response of Dunaliella salina

    Jun Zhou, Haiqing Sun, Jiali Wei, Ping Li*

    Phyton-International Journal of Experimental Botany, Vol.90, No.5, pp. 1401-1414, 2021, DOI:10.32604/phyton.2021.016514

    Abstract The halophilic unicellular green alga Dunaliella salina is characterized as the distinct tolerance to high salt in the field. Normally the organism is concurrently subjected with salt and alkali stress, but the feature of D. salina in response to high alkali stress was not investigated before. In this study, we used high pH stress to simulate high alkali stress and found D. salina also showed tolerance to high alkali stress, even grown under pH at 11.0, with high cell density and chlorophyll biosynthesis. High-throughput transcriptome analysis revealed the dramatically different expression of genes associated with nitrogen metabolism, lipid… More >

  • Open Access

    REVIEW

    Carotenoids: New Applications of “Old” Pigments

    Stefan M. Kolašinac1, Zora P. Dajić Stevanović1,*, Sofija N. Kilibarda1,  Aleksandar Ž. Kostić2,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1041-1062, 2021, DOI:10.32604/phyton.2021.015996

    Abstract Carotenoids represent a large group of mainly red, orange, and yellow natural metabolites mainly involved in regulation of many metabolic processes. Carotenoids are beneficial for human health. Current study describes the importance, chemical composition and functioning of carotenoids. It is well known that carotenoids support pigments acting in light absorbance mechanisms during photosynthesis, and are known to protect the chlorophyll molecules from oxidative stress and reactive oxygen species (ROS) damage. Carotenoids are involved in signaling processes in plants, responses to environmental stresses, pollination, germination and reproduction, and development regulation. As nutrients of strong antioxidant activity… More >

  • Open Access

    ARTICLE

    Nanoparticles induce the biosynthesis and activity of the new possible therapeutic proteinase source, Talaromyces purpureogenus KJ584844

    SALLY NEGM1,2, MOHAMMAD EL-METWALLY3, WESAM ELDIN SABER4, SAHAR ABO-NEIMA5, MAHMOUD MOUSTAFA6,7,*, ATTALLA EL-KOTT6,8

    BIOCELL, Vol.45, No.1, pp. 119-127, 2021, DOI:10.32604/biocell.2021.012011

    Abstract The need for the bacterial proteinase is rapidly growing, urging to catch a lowcost medium for the microbial fermentation, nanoparticles can play a vital role in this respect. The proteinase of Talaromyces purpureogenus was produced on the tubers of Helianthus tuberosus that also operated as solid support for the fermentation process. The interface amongst nitrogen sources (NH4Cl and yeast extract) was investigated, applying the statistical modeling of central composite design under solid-state fermentation. The optimum medium for proteinase secretion was stimulated by 979.82 mg NH4Cl and 437.68 mg yeast extract per 100 g substrate, yielding 108.15 U/g tubers.… More >

  • Open Access

    ARTICLE

    DWARF and SMALL SEED1, a Novel Allele of OsDWARF, Controls Rice Plant Architecture, Seed Size, and Chlorophyll Biosynthesis

    Yan Li1, Renquan Huang1, Jianrong Li1, Xiaozhen Huang1, Xiaofang Zeng1,*, Degang Zhao1,2,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 111-127, 2021, DOI:10.32604/phyton.2020.013933

    Abstract Plant architecture is a vital agronomic trait to control yield in rice (Oryza sativa L.). A dwarf and small seed 1 (dss1) mutant were obtained from the ethyl methanesulfonate (EMS) mutagenized progeny of a Guizhou glutinous landrace cultivar, Lipingzabianhe. The dss1 mutant displayed phenotypes similar to those of brassinosteroid (BR) deficient mutants, such as dwarfing, dark green and rugose erect leaves, small seeds, and loner neck internode panicles with primary branching. In our previous study, the underlying DSS1 gene was isolated, a novel allele of OsDWARF (OsBR6ox) that encodes a cytochrome P450 protein involved in the BR biosynthetic pathway by More >

  • Open Access

    REVIEW

    Flesh Color Diversity of Sweet Potato: An Overview of the Composition, Functions, Biosynthesis, and Gene Regulation of the Major Pigments

    Hanna Amoanimaa-Dede, Chuntao Su, Akwasi Yeboah, Chunhua Chen, Shaoxia Yang, Hongbo Zhu*, Miao Chen*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 805-833, 2020, DOI:10.32604/phyton.2020.011979

    Abstract Sweet potato is a multifunctional root crop and a source of food with many essential nutrients and bioactive compounds. Variations in the flesh color of the diverse sweet potato varieties are attributed to the different phytochemicals and natural pigments they produce. Among them, carotenoids and anthocyanins are the main pigments known for their antioxidant properties which provide a host of health benefits, hence, regarded as a major component of the human diet. In this review, we provide an overview of the major pigments in sweet potato with much emphasis on their biosynthesis, functions, and regulatory More >

  • Open Access

    ARTICLE

    Elemental sulfur upregulated testicular testosterone biosynthesis by associating with altered gut microbiota in mice

    YUAN BI1,2,#, TIANQI LI2,#, HONGJIE PAN2, MING GUO2, LIANGKANG CHEN2, QI QI1, MEIXIN ZHANG2, LINGLING ZHANG2, LININ YU2, XIAOFENG WAN2, HUAJUN ZHENG2,*, RUNSHENG LI2,*

    BIOCELL, Vol.44, No.3, pp. 301-313, 2020, DOI:10.32604/biocell.2020.011208

    Abstract Elemental sulfur has been used as a traditional Chinese medicine to treat the late-onset hypogonadism and impotence without a clarified mechanism for many hundreds of years. In the present study, mice were received sulfur or distilled water for 35 days by daily intragastric gavage at a dose of 250 mg/kg body weight. Then, the serum testosterone level and genes associated with testicular testosterone biosynthesis (TTB) were detected. The gut microbiota was also analyzed by 16S rRNA gene sequencing. Serum testosterone level was significantly increased by 291.1% in sulfur-treated mice. The H2S levels in serum and feces… More >

  • Open Access

    ARTICLE

    In Vitro Propagation, Isolation and Expression Studies of Suaeda edulis Genes Involved in the Osmoprotectants Biosynthesis

    Gloria Viviana Cerrillo-Rojas1, Mariana Tiscareño-Andrade1, Ana Erika Ochoa-Alfaro2, Eugenio Pérez-Molphe Balch1, Ruth Elena Soria-Guerra2, José Francisco Morales-Domínguez1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 715-726, 2020, DOI:10.32604/phyton.2020.09769

    Abstract Halophytes are an excellent choice for the study of genes conferring salt tolerance to salt-sensitive plants and, they are suitable for reclamation and remediation of saline soil. We develop an in vitro plant propagation protocol and studies of genes involved with GB and Pro biosynthesis in Suaeda edulis. Axillary buds were used as explants and cultured in different treatments on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of plant growth regulators. The highest number of multiple shoots was on MS medium containing 1 mg/L Benzyladenine (BA) and / or 2 g/L activated… More >

  • Open Access

    ARTICLE

    Improved Permeability Properties for Bacterial Cellulose/ Montmorillonite Hybrid Bionanocomposite Membranes by In-Situ Assembling

    Itxaso Algar1, Clara Garcia-Astrain1, Alba Gonzalez2, Loli Martin3, Nagore Gabilondo1, Aloña Retegi1*, Arantxa Eceiza1*

    Journal of Renewable Materials, Vol.4, No.1, pp. 57-65, 2016, DOI:10.7569/JRM.2015.634124

    Abstract Bacterial cellulose/montmorillonite (BCMMT) hybrid bionanocomposite membranes were prepared by in-situ assembling or one-step biosynthesis process. The presence of MMT in BC membranes was confi rmed by thermogravimetric analysis and quantifi ed by mass spectrometry, resulting in bionanocomposites with MMT contents between 7–13 wt%. The incorporation of MMT during BC biosynthesis modifi ed BC morphology and led to lower porosity, even though higher water holding capacity was achieved. Bionanocomposites showed improved thermal stability and water vapor and oxygen gas barrier properties up to 70 and 80% with respect to neat BC membranes. This improvement was related More >

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