CHUNJIAO LIU^1,2, QINHONG KONG^1,2, FENG PAN^1,2, SHAN JIANG^1,2, LINGJIE MENG^1,2, GAI HUANG^1,2, LIDAN LU^1,2, SANHUA LI^1,2,*, YUN LIU^1,2,3,*

BIOCELL, Vol.45, No.4, pp. 943-951, 2021, DOI:10.32604/biocell.2021.015836 - 22 April 2021

Abstract Traditional Chinese medicine (TCM) has been increasingly applied in both preventing and treating a variety of cancers in the last decades, attributing to its fewer side effects as compared with chemotherapy drugs. Hellebrigenin, a component of Chanpi from the skin of Bufo bufogargarizans Cantor or Duttaphrynus melanostictus has been reported to have an obvious anti-cancer activity on various cancers. However, the effect and mechanism of hellebrigenin on colorectal cancers were still unknown. Herein, the present study demonstrated hellebrigenin significantly reduced viability and triggered apoptosis via the intrinsic pathway in colorectal cancer cell lines HCT116 and HT29 in More >

SAYEQUA DANDOTI^*

BIOCELL, Vol.45, No.4, pp. 863-884, 2021, DOI:10.32604/biocell.2021.013993 - 22 April 2021

Abstract Inactivation of apoptosis is the prime phenomenon in cancer development and cancer treatments. Mutations in the apoptotic pathway not only exert resistance to apoptosis and provide a survival advantage to cancer cells but also confer resistance to cancer therapies. Escaping apoptosis is the “hallmark” of cancer cells. Cancer cells can withstand many apoptotic stimuli, such as DNA damage, unfavorable environments, and cytotoxic therapies. Substantial research has been carried out and is in good progress on the various mechanisms adopted by cancer cells to evade apoptosis. This article reviews the apoptosis escape mechanisms by cancer cells, More >

Erkan Arslan^1,*, Ismail Koyuncu²

Oncologie, Vol.23, No.1, pp. 105-117, 2021, DOI:10.32604/Oncologie.2021.014764 - 30 March 2021

Abstract Prostate cancer is the second most common cancer in men. Prostate-specific antigen (PSA) levels, commonly used in the diagnosis of prostate cancer, are increased in both malign and benign conditions, such as prostate hyperplasia (BPH) and prostatitis. Thus, more specific markers are urgently needed to discriminate between prostate cancer and benign diseases of the prostate. The purpose of this study is to examine both the intracellular and extracellular free amino acid profiles of metastatic prostate cancer cells (PC-3), normal prostate cells (PNT-1A), and metabolic changes (e.g., pH). In this study, cancer and normal cells were… More >

SUSHMITHA SRIRAMULU¹, SARUBALA MALAYAPERUMAL¹, SUMAN K. NANDY², ANTARA BANERJEE¹, MUSTHAFA MOHAMED ESSA^3,4, SARAVANABABU CHIDAMBARAM⁵, M. WALID QORONFLEH^6,7, SURAJIT PATHAK^1,*

BIOCELL, Vol.45, No.3, pp. 563-576, 2021, DOI:10.32604/biocell.2021.014756 - 03 March 2021

Abstract Colon cancer is the third major cause of cancer deaths, accounting for about 8% in terms of mortality globally. The present study aims to explore the effect of silencing Astrocyte Elevated Gene-1 (AEG-1), a metastasis mediating factor, and how it interacts with Exostosin-1 (EXT-1) protein to inhibit the proliferative and invasive potential in colon cancer cells. Forward siRNA transfection was performed using AEG-1 siRNA in SW480 and SW620 colon cancer cell lines, and the expression levels of mRNA and protein were analyzed by Real-time PCR and Immunofluorescence. A simple bioinformatics approach was carried out to… More >

DAN ZHANG¹, HAIJING LIU¹, ZHENNAN YI², YUANYUAN LU³, YANYAN CHEN⁴, WEIQIANG SU², HUIBING LIN², ZHIHUI ZHANG^5,*, WEI LEI^6,*

BIOCELL, Vol.45, No.3, pp. 617-625, 2021, DOI:10.32604/biocell.2021.012504 - 03 March 2021

Abstract Chemotherapy drug resistance is the main cause leading to the relapse and metastasis of non-small cell lung cancer (NSCLC) patients. Our study aimed to investigate the mechanism of pemetrexed resistance in NSCLC. Firstly, the pemetrexed (PEM)-resistant PC-9 and A549 lung adenocarcinoma cell lines (PC-9/PEM and A549/PEM) were established. The expression of thymidylate synthase (TS) in PC-9/PEM, A549/PEM, A549, and PC-9 cells were analyzed by qRT-PCR and western blot. Then, cell viability, colony formation, migration, and invasion were performed on PEM-resistant cells transfected with TS siRNA. The role of EGFR in PEM resistance of PEM-resistant cells… More >

JI ZHU^#, XIYING BO^#, GENGXI JIANG, SHIHUA YAO, TIEJUN ZHAO^*, LING CHEN^*

BIOCELL, Vol.45, No.3, pp. 639-647, 2021, DOI:10.32604/biocell.2021.012847 - 03 March 2021

Abstract Non-small cell lung cancer (NSCLC) cells intake and consume glucose at high efficiency by aerobic glycolysis to maintain robust cell growth and resist cell death. MicroRNAs (miRNAs) have been known to play pivotal roles in NSCLC development partly through mediating glycolysis. However, only a few miRNAs have been experimentally confirmed as critical regulators of glycolysis in NSCLC. TCGA datasets were analyzed to screen for differentially expressed miRNAs between NSCLC and normal tissues. The function of miR-1294-5p was determined in NSCLC cells by cell proliferation, glucose uptake, lactate release, and Extracellular Acidification Rate (ECAR) assays. The… More >

MAYSON H. ALKHATIB^1,2,*, SALWA M. AL-HASHEMI¹, HANA M. GASHLAN¹

BIOCELL, Vol.45, No.3, pp. 695-703, 2021, DOI:10.32604/biocell.2021.014349 - 03 March 2021

Abstract Incorporation of etoposide (ETP) into nanoemulsion (NE) containing polyunsaturated fatty acids (PUFAs) may potentially augment its antiproliferation effect on the cancer cells. The current study aimed to examine the in vitro antitumor activity of a novel formulation (ETP-BC/EP-NE) produced by combining the anticancer drug (ETP) with NE (BC/EP-NE) consisting of the black currant seed and organic evening primrose oils. The produced formulas were physically characterized using zetasizer measurements. Their cytotoxic effect was testified at concentrations ranges from 0.0001 to 5 μM using CCK-8. Apoptotic and anti-invasion effects were evaluated using the assays of mitochondrial membrane potential,… More >

ATTALLA EL-KOTT^1,2, EMAN ELBEALY³, FAHMY ELSAID^1,4, HAITHAM EL-MEKKAWY¹, ABD-EL-KARIM ABD-LATEIF⁵, ABDULALI TAWEEL⁶, HEBA KHALIFA², AHMAD KANDEEL⁵, KAREEM MORSY^1,7, ESSAM IBRAHIM^1,8,9, MASHAEL MOHAMMED BIN-MEFERIJ^10,*

BIOCELL, Vol.45, No.1, pp. 89-101, 2021, DOI:10.32604/biocell.2021.012516 - 26 January 2021

Abstract This study investigates if the anti-tumor effect of Pterostilbene in the SKOV3 ovarian cancer (OC) cell line involves inhibition of cell metabolism and tested in this effect involves modulating AMPK and Akt-induced regulation of mTORC1. Initially, SKOV3 cells were cultured in the humidified conditions in DMEM media for 24 h with or without increasing concentration of Pterostilbene. Then, the cells were incubated with Pterostilbene (IC₅₀ = 50 µM) under similar conditions with or without pre-incubation with Dorsomorphin, an AMPK inhibitor. In a dose-dependent manner, Pterostilbene inhibited SKOV3 cell survival and increased their lysate levels of lactate… More >

Huifang Lv, Honglin Hou, Huijun Lei, Caiyun Nie, Beibei Chen, Liangyu Bie, Lili Han, Xiaobing Chen

Oncology Research, Vol.28, No.3, pp. 225-236, 2020, DOI:10.3727/096504019X15753718797664

Abstract S100 binding protein A16 (S100A16) expression levels are closely associated with microRNA (miRNA) processing. Higher levels of S100A16 are reported during the progression of many cancers. Our study mainly explored the interaction between S100A16 and miR-6884-5p in gastric cancer (GC). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the level of S100A16 and miR-6884-5p in GC tissues and cell lines. The si-S100A16, pcDNA-S100A16, miR-6884-5p mimic or inhibitor was transfected into GC cells, and the effects of S100A16 and miR-6884-5p on the proliferation, invasion, and epithelial–mesenchymal transition (EMT) were explored by qRT-PCR and Western… More >

Yangmei Zhang^*1, Xichang Zhou^†1, Long Cheng^†, Xiang Wang^*, Qinglin Zhang^‡, Youwei Zhang^*, Sanyuan Sun^*

Oncology Research, Vol.28, No.3, pp. 213-223, 2020, DOI:10.3727/096504019X15668125347026

Abstract PRKAA1 (protein kinase AMP-activated catalytic subunit 1) is a catalytic subunit of AMP-activated protein kinase (AMPK), which plays a key role in regulating cellular energy metabolism through phosphorylation, and genetic variations in the PRKAA1 have been found to be associated with gastric cancer risk. However, the effect and underlying molecular mechanism of PRKAA1 on gastric cancer tumorigenesis, especially the proliferation and apoptosis, are not fully understood. Our data showed that PRKAA1 is highly expressed in BGC- 823 and MKN45 cells and is expressed low in SGC-7901 and MGC-803 cells in comparison with the other gastric… More >