Xinxiong FEI, Wenbin HU, Gangsheng WANG, Chunyan SU, Xuqun HUANG, Zhongjun JIANG^*

BIOCELL, Vol.44, No.1, pp. 81-88, 2020, DOI:10.32604/biocell.2020.08125

Abstract Lung cancer poses a serious threat to human life with high incidence and miRNA is an important biomarker in tumors. This study aimed to explore the effect of miR-143-3p on the biological function of lung cancer cells and the underlying mechanism. Eighty-seven samples of lung cancer tissues and 81 samples of tumor-adjacent tissues from patients undergoing radical lung cancer surgery in our hospital were collected. The lung cancer cells and lung fibroblast cells (HFL-1) were purchased, and then miR-143-3p-mimics, miR-NC, si-CTNND1, and NC were transfected into A549 and PC-9 cells to establish cell models. MiR-143-3p and CTNND1 expression levels were… More >

Wensong LIU¹, Yunjie LU¹, Dong ZHANG¹, Longqing SHI¹, Guangchen ZU¹, Haijiao YAN^2,*, Donglin SUN^1,*

BIOCELL, Vol.44, No.1, pp. 73-80, 2020, DOI:10.32604/biocell.2020.08613

Abstract Pancreatic cancer is one of the most aggressive malignancies with poor prognosis and high mortality. Recent studies showed that microRNAs are dysregulated and involved in the initiation and progression of pancreatic cancer. In this study, we found that miR-708 was significantly downregulated in pancreatic cancer tissues and cell lines. Lentivirus-mediated overexpression of miR-708 could significantly inhibit the proliferation and invasion, while enhanced chemosensitivity to gemcitabine in both Panc-1 and SW1990 cells. Luciferase reporter assay showed that miR-708 bound the 3’-untranslated region of survivin and suppressed the expression of survivin in pancreatic cancer cells. In pancreatic cancer tissues, survivin protein was… More >

T.H. Satoh², T.A. Surmacz³, O. Nyormoi⁴, C.M. Whitacre¹

BIOCELL, Vol.27, No.1, pp. 47-55, 2003, DOI:10.32604/biocell.2003.27.047

Abstract This study shows a strong association between cell attachment to substratum and activation of β1-integrin-signaling with resistance to the camptothecin derivative topotecan (TPT) in breast cancer cells. We propose a mechanistic-driven approach to sensitize the cells to camptothecins. ZR-75-1 anchoragedependent breast cancer cell line, its derivative 9D3S suspension cells (9D3S-S), and 9D3S cells attached to fibronectin-coated plates (9D3S-A) were treated with TPT (1 µM) or CPT-11 (40 µM) for 48 h. Programmed cell death (PCD), as shown by poly(ADP-ribose) polymerase (PARP), pro-caspase-3 and pro-caspase-9 cleavage, was observed in 9D3S-S cells but not in ZR-75-1 or 9D3S-A cells. Because p125 focal… More >

Dairong LI¹, Xianlu ZHUO^1,2, Lumi HUANG¹, Xiaohui JI¹, Donglin WANG¹

BIOCELL, Vol.43, No.3, pp. 139-144, 2019, DOI:10.32604/biocell.2019.06460

Abstract X-ray repair cross-complementing protein 1 (XRCC1) could repair cisplatin-induced DNA damage. XRCC1 Arg399Gln and Arg194Trp variants alter XRCC1 expression and function, leading to changes in cancer sensitivity to cisplatin treatment. This study aimed to investigate the effects of XRCC1 Arg399Gln and Arg194Trp polymorphisms on cell viability, apoptosis and XRCC1 expression in cisplatin-sensitive A549 and cisplatin-resistant A549/DDP nonsmall cell lung cancer (NSCLC) cells. Plasmids carrying XRCC1 Arg399Gln and Arg194Trp were constructed and transfected into A549 and A549/DDP cells. RT–PCR, Western blot, MTT assay, and flow cytometry analysis were performed to assess cell viability, apoptosis, and XRCC1 expression. Compared to control cells,… More >

Moharam Habibnejad Korayem^1,*, Zahra Rastegar²

Molecular & Cellular Biomechanics, Vol.16, No.2, pp. 109-122, 2019, DOI:10.32604/mcb.2019.04706

Abstract The mechanical properties of single cells have been recently identified as the basis of an emerging approach in medical applications since they are closely related to the biological processes of cells and human health conditions. The problem in hand is how to measure mechanical properties in order to obtain them more accurately and applicably. Some of the cell’s properties such as elasticity module and adhesion have been measured before using various methods; nevertheless, comprehensive tests for two healthy and cancerous cells have not been performed simultaneously. As a Nanoscale device, AFM has been used for some biological cells, however for… More >

Zahra Niki Boroujeni¹, Atefeh Shirkav¹, Seyed Ahmad Aleyasin^1,*

Molecular & Cellular Biomechanics, Vol.16, No.1, pp. 41-58, 2019, DOI:10.32604/mcb.2019.04366

Abstract Today, prognosis, diagnosis and treatment of cancers are progressing with non-invasive methods, including investigation and modification of the DNA methylation profile in cancer cells. One of the effective factors in regulating gene expression in mammals is DNA methylation. Methylation alterations of genes by external factors can change the expression of genes and inhibit the cancer. In the present study, we investigated the effect of Down syndrome critical region 1 gene (DSCR1) ectopic expression on the methylation status of the BCL-XL, ITGA6, TCF3, RASSF1A, DOK7, VIM and CXCR4 genes in breast cancer cell lines. The effect of DSCR1 ectopic expression on… More >

Zahra Niki Boroujeni¹, Atefeh Shirkav¹, Seyed Ahmad Aleyasin^1,*

Molecular & Cellular Biomechanics, Vol.15, No.4, pp. 215-227, 2018, DOI:10.32604/mcb.2018.01813

Abstract Down syndrome critical region 1 gene (DSCR1) is an anti-angiogenesis gene that inhibits the growth of tumor cells. In this study, the role of autophagy and apoptosis in DSCR1-induced cytotoxicity were investigated in MDA-MB-468 breast cancer cells. Lentivirus vector harboring DSCR1 (LV-DSCR1⁺) was constructed in HEK 293 cells and the optimal dosage of lentivirus vector for infection was determined by the MTT assay. After infection of cells using LV-DSCR1⁺, acridine orange and ethidium bromide staining was performed to investigation of apoptosis and autophagy. Expression of DSCR1 and marker genes for angiogenesis (VEGF), apoptosis (Bax and Bcl2) and autophagy (LC3 and… More >