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  • Open Access

    ARTICLE

    Optimized enzyme-assisted microwave extraction and potential inhibitory action against α-glucosidase of polysaccharides from sweet corncobs

    Ma YQ, X Wang, SH Gao, J Zhang

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 79-86, 2016, DOI:10.32604/phyton.2016.85.079

    Abstract The conditions for extracting polysaccharides from sweet corncobs (SCP) were studied. Four parameters (ratio of water to raw material, compound enzyme concentration, temperature of enzymolysis and duration of enzymolysis) affecting the extraction of SCP were optimized using response surface methodology (RSM). Under the optimized conditions, the yield of SCP was 17.58 %. SCP had inhibitory effects on α-amylase and α-glucosidase activities, and the IC50 was 20.91 mg/mL and 12.47 mg/mL. SCP may therefore have prevention and treatment effects on postprandial hyperglycemia in diabetes. The inhibitory effects of SCP were improved after fractionation, and were strongest in the fraction SCP80. The… More >

  • Open Access

    ARTICLE

    Seed morphology, presence of areoles and water entry at imbibition of Vicia dasycarpa Ten. cv. Tolse F.C.A

    Perissé P1 y GM Tourn2

    Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 184-189, 2015, DOI:10.32604/phyton.2015.84.184

    Abstract The morphological characteristics of the seeds of Vicia dasycarpa Ten. cv. Tolse FCA were studied in relation to the area of imbibition water entry and were considered the presence of areoles. Seeds were analyzed using a stereo, optical and scanning electron microscope (SEM). The determination of the initial water entry area was carried out by immersing the seeds in a solution of tetrazolium (1%). This study showed that this species has seeds with a halo framing the hilum, an inconspicuous dry aril and a deltoid micropyle. The seedcoat pattern is papillose. The tracheid bar is surrounded by a ring of… More >

  • Open Access

    ARTICLE

    Study of the mitotic and meiotic chromosomes of sotol (Dasylirion cedrosanum Trel.)

    Hernández-Quintero JD1, MH Reyes-Valdés2, DV Mendoza-Rodríguez3, M Gómez-Martínez2, R Rodríguez-Herrera4

    Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 107-112, 2015, DOI:10.32604/phyton.2015.84.107

    Abstract The genus Dasylirion is a group of plants typically present in the Chihuahuan Desert, perennial, with a dioecious sexual behavior and commonly called sotoles. This genus has been little studied from the biological point of view, and the bases of its reproductive response remain unknown. In this work we studied the chromosome number and meiotic response of Dasylirion cedrosanum in the county of Saltillo, Coahuila, located at the North East of Mexico. For the preparation of mitotic chromosomes, we used a technique based on enzymatic treatment with pectolyase and cellulase, as well as staining with acetocarmin dye. For the study… More >

  • Open Access

    ARTICLE

    First report of Tobacco mosaic virus (TMV) and Poinsettia mosaic virus (PnMV) in poinsettia (Euphorbia pulcherrima Willd. Ex Klotzch) in Mexico

    Ocampo Ocampo T1, DL Ochoa Martínez1, S Ramírez Rojas2, G Valdovinos Ponce1, C Nava Díaz1

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 235-241, 2013, DOI:10.32604/phyton.2013.82.235

    Abstract The Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), in Zacatepec Morelos, started a genetic improvement program of wild and semi-cultivated poinsettia plants ("nochebuena de sol"). It is important to generate a phytosanitary database that allows the release of new varieties and hybrids that provide tolerance to the major pathogens reported in cultivated poinsettia plants. The phytosanitary status of the “nochebuena de sol” plants is not available in Mexico, so the objective of this research was to generate preliminary data about viruses associated to these materials. Based on DAS-ELISA and RT-PCR results, we reported the presence of the Tobacco… More >

  • Open Access

    ARTICLE

    Anti-inflammatory activity of Ziziphus amole

    Romero-Castillo PA1, MC Pérez Amador Barron1, P Guevara Fefer1, V Muñoz Ocotero1, A Reyes Dorantes2, F Aguirre Garcia2, A Amaya Chavez3

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 75-80, 2013, DOI:10.32604/phyton.2013.82.075

    Abstract The search for new anti-inflammatory drugs with fewer side effects than those currently available in the market has recently attracted the interest of scientists. Medicinal plants might be a natural source of medicines. Such is the case with Ziziphus amole (Sessé & Moc.) M.C. Johnst. or corongoro, a plant species native to México. It is used in traditional medicine as an anti-inflammatory drug, a healing substance and an analgesic, and in the treatment of diarrhea, diabetes, asthma and hemorrhoids. The aim of this study was to evaluate the anti-inflammatory effect of Z. amole in the acute 12-O-tetradecanoylphorbol-13-acetate (TPA) induced mouse… More >

  • Open Access

    ARTICLE

    Chromosomal location of four genes encoding Class III peroxidases in wheat

    Simonetti E1, E Alba2, A Delibes2

    Phyton-International Journal of Experimental Botany, Vol.81, pp. 139-142, 2012, DOI:10.32604/phyton.2012.81.139

    Abstract In a previous work, deduced amino acid sequences from twenty wheat peroxidase genes were assigned to seven groups designated as TaPrx108 to TaPrx114. Some of these apoplastic peroxidases have previously shown to play different roles in the plant defense responses to infection by the cereal cyst nematode Heterodera avenae. In the present study, PCR marker analysis using Sears’s aneuploid wheat lines cv. ‘Chinese Spring’ was used to locate four genes encoding peroxidase isozymes. The TaPrx111-A, TaPrx112-D and TaPrx113-F genes were located on the short arm of chromosome 2B and the TaPrx109-C on the long arm of chromosome 1B. These results… More >

  • Open Access

    ARTICLE

    Sex ratio and spatial distribution of pistillate and staminate plants of Dasylirion cedrosanum

    Reyes-Valdés MH, JD Hernández-Quintero1, YA Morales-Reyes2, DV Mendoza-Rodríguez, DU González-Uribe, F Ramírez-Godina, JA Villarreal-Quintanilla

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 171-180, 2017, DOI:10.32604/phyton.2017.86.171

    Abstract The genus Dasylirion forms a group of well-represented perennial, dioecious species called “sotoles” in Mexico. This genus is a main component of the arid lands of northern Mexico and southern United States, and plays important ecological and economic roles. Four D. cedrosanum populations from northeastern Mexico were surveyed to gather statistics related to sex ratios and spatial distribution. The statistics were performed on an unbiased sample of 448 plants. Tests for sex ratio and spatial distribution were carried out. No evidence of departure from a 1:1 sex ratio proportion was found, both at the global level and within sampling sites.… More >

  • Open Access

    ARTICLE

    The Volatility of High-Yield Bonds Using Mixed Data Sampling Methods

    Maojun Zhang1,2, Jiajin Yao1, Zhonghang Xia3, Jiangxia Nan1,*, Cuiqing Zhang1

    CMC-Computers, Materials & Continua, Vol.61, No.3, pp. 1233-1244, 2019, DOI:10.32604/cmc.2019.06118

    Abstract It is well known that economic policy uncertainty prompts the volatility of the high-yield bond market. However, the correlation between economic policy uncertainty and volatility of high-yield bonds is still not clear. In this paper, we employ GARCH-MIDAS models to investigate their correlation with US economic policy uncertainty index and S&P high-yield bond index. The empirical studies show that mixed volatility models can effectively capture the realized volatility of high-yield bonds, and economic policy uncertainty and macroeconomic factors have significant effects on the long-term component of high-yield bonds volatility. More >

  • Open Access

    ABSTRACT

    Identification of Lysyl Oxidase on Repression of Inflammation for Promoting Anterior Cruciate Ligament Remodeling

    Yan Gao1, Chunli Wang1, Li Yang1,*

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 93-93, 2019, DOI:10.32604/mcb.2019.07322

    Abstract At present, anterior cruciate ligament (ACL) damage repair is still a huge challenge. Our previous studies indicated that the Lysyl oxidase (LOX) were significantly reduced in injurious ACL fibroblasts, which is the major reason for its poor healing ability. The main purpose of our study was to detected the potential of LOX to act as an anabolic agent in injured ACL. The effect of LOX on the ACL at a concentration of 20ng/mL was investigated. The molecular mechanisms and signaling pathway were elucidated by RNA-sequencing, q-PCR and western blotting. For the in vivo study, the LOX was injected into the… More >

  • Open Access

    ABSTRACT

    LOX Alleviates Rat Intervertebral Disc Degeneration Through ECM Improvement and Anti-Apoptotic Protection in Nucleus Pulposus Cells

    Runze Zhao1, Tingting Xia1, Mengyue Wang1, Fan Feng1, Wanqian Liu1,*, Li Yang1,*

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 77-77, 2019, DOI:10.32604/mcb.2019.07155

    Abstract This study was focus on the exploring the therapeutic function of lysyl oxidase (LOX) in rat nucleus pulposus (NP) cells in intervertebral disc degeneration (IVDD). To do this, a Sprague-Dawley (SD) rat caudal spine degeneration model was established by puncturing the Co5-6 disc. NP cells apoptosis and extracellular matrix (ECM) degeneration in IVDD were evaluated by real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR), Hematoxylin-Eosin (H&E) and immunofluorescence. Then, the therapeutic effect of LOX on IVDD was evaluated by histological staining. In vitro, the regulator effect of LOX on degenerate rat NP cell was explored. ECM relate proteins and cytokines were… More >

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