ELEONIDAS MOURA LIMA^1,2, MARIANA FERREIRA LEAL³, MARÍLIA DE ARRUDA CARDOSO SMITH³, ROMMEL RODRÍGUEZ BURBANO⁴, PAULO PIMENTEL DE ASSUMPÇÃO⁵, MARIA JOSE BELLO⁶, JUAN ANTONIO REY⁶, FRANCINALDO FERREIRA DE LIMA⁷, CACILDA CASARTELLI²

BIOCELL, Vol.32, No.3, pp. 237-243, 2008, DOI:10.32604/biocell.2008.32.237

Abstract Gastric cancer is one of the most common malignancies. DNA methylation is implicated in DNA mismatch repair genes deficiency. In the present study, we evaluated the methylation status of MLH1, MSH2, MSH6 and PMS2 in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosal of gastric cancer patients from Northern Brazil. We found that none of the nonneoplastic samples showed methylation of any gene promoter and 50% of gastric cancer samples showed at least one methylated gene promoter. Methylation frequencies of MLH1, MSH2, MSH6 and PMS2 promoter were 21.74%, 17.39%, 0% and 28.26% respectively in gastric… More >

ZONG-YUN LI^1,2*, MEI-LI FU¹, FANG-FANG HU¹, SHU-FENG HUANG¹, YUN-CHUN SONG²

BIOCELL, Vol.30, No.1, pp. 27-32, 2006, DOI:10.32604/biocell.2006.30.027

Abstract The ribosomal DNA (45S rDNA) behaviors during the cell cycle were analyzed on interphase nuclei, prophases, metaphases, pachytene chromosomes and extended DNA fibers in rice (Oryza,sativa ssp.indica cv.Guangluai No.4) by using high-resolution fluorescent in situ hybridization (FISH). The results show that 45S rDNA is located at the ends of short arms of chromosomes 9 and 10. But the signals are much more intense on chromosome 9 than on chromosome 10 in metaphase. Pachytene chromosome has rDNA signal arrays on chromosome 9. Different phases are described and discussed. These results indicate that the activity of rDNA at individual loci may also… More >

ZHONGHAI CHEN¹, YONGZHEN PANG¹, XIAOJUN LIU¹, XINGLONG WANG¹, ZHONGXIANG DENG¹, XIAOFEN SUN¹, KEXUAN TANG^1,2

BIOCELL, Vol.29, No.2, pp. 187-193, 2005, DOI:10.32604/biocell.2005.29.187

Abstract Using RNA extracted from Zantedeschia aethiopica young leaves and primers designed according to the conservative regions of Araceae lectins, the full-length cDNA of Z. aethiopica agglutinin (ZAA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of zaa was 871 bp and contained a 417 bp open reading frame (ORF) encoding a lectin precursor of 138 amino acids. Through comparative analysis of zaa gene and its deduced amino acid sequence with those of other Araceae species, it was found that zaa encoded a precursor lectin with signal peptide. Secondary and three-dimensional structure analyses showed that ZAA had… More >

A. DEL CAMPO*, M. BRACHO, L. MARCANO, J. GUÍÑEZ, C. DE LA TORRE**

BIOCELL, Vol.29, No.2, pp. 169-176, 2005, DOI:10.32604/biocell.2005.29.169

Abstract This work evaluated the qualitative and quantitative cellular changes induced by treatment with 5-aminouracil (5-AU) and a combination of 5-AU and caffeine in plant cells in relation to DNA damage, repaired damage, and residual damage. As biological material, Allium cepa L. root tips were used, grown in filtered water, in darkness, with aeration at constant temperature of 25 °C ± 0.5. Cell populations were synchronized using 5 mM caffeine in order to study the effects of 5-AU and caffeine/5-AU combined treatment on the DNA content and their incidence in the entrance to mitosis. The results showed a delay in the… More >

CARLOS A. MARTÍN, JOSÉ MIGUEL SURUR, MARCELA N. GARCÍA, FÉLIX CORRONS, AMADO F. BADRÁN

BIOCELL, Vol.29, No.1, pp. 33-38, 2005, DOI:10.32604/biocell.2005.29.033

Abstract Variations in DNA synthesis (DNAs) and Nucleolar Organizer Regions (NORs) were studied in the littoral cell population from regenerating liver of C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections. Tissue samples were obtained every four hours from the 30th to the 54th hours after a partial hepatectomy. The results showed, in both parameters, a gradual increment of the values during the period studied, with highest values (DNAs 107.1 ± 16.1 SE; AgNORs 77.3… More >

PABLO A. VALDECANTOS, MARTÍN E. ARGAÑARAZ, CARLOS M. ABATE, DORA C. MICELI

BIOCELL, Vol.28, No.3, pp. 287-297, 2004, DOI:10.32604/biocell.2004.28.287

Abstract As a step towards the identification of genes preferentially expressed in the oviduct during early rat embryo development, we isolated a cDNA fragment (Pr14) by using RNA arbitrarily primed PCR (RAP-PCR), being its expression restricted to oviduct and uterus; its mRNA is mainly expressed in oviduct during late luteal phase and early pregnancy. This fragment is 100% identical to a rat DNA sequence (Accession No. NW_047400) downstream the terminal exon of a Rattus norvegicus gene (Locus Link Accession No. LOC289316) similar to ebaf (endometrial bleeding-associated factor), a novel member of the Transforming Growth Factor superfamily. Northern analyses showed that this… More >

Vital-López L, MA Cruz-Hernández, S Fernández-Dávila, A Mendoza-Herrera

Phyton-International Journal of Experimental Botany, Vol.85, pp. 210-217, 2016, DOI:10.32604/phyton.2016.85.210

Abstract Genetically modified crops could cause negative effects on bacterial communities. In this study, we compared the bacterial community structure of two maize cultivars to determine whether the transgenic cultivar exerts a negative effect on bacterial communities inhabiting the rhizosphere. Cultivars included the genetically modified maize (Zea mays), with the pat-gene conferring resistance to the herbicide glufosinate (synonym: L-phosphinothricin), and the hybrid, conventional maize. Metagenomic DNA was extracted from the rhizosphere of plants grown in a greenhouse. Single-strand conformation polymorphism, based on polymerase chain reaction amplifying a partial subunit rRNA gene was used to characterize and generate genetic profiles that corresponded… More >

Galindo-Tovar ME, VS Gasperin-Sanatta, JA Alejandre-Rosas, OR Leyva-Ovalle, J Murguía-González, HE Lee-Espinosa, I L,ero-Torres

Phyton-International Journal of Experimental Botany, Vol.83, pp. 307-310, 2014, DOI:10.32604/phyton.2014.83.307

Abstract Sechium edule is an important crop in Southern Mexico and Central America, and México was the main producer, with 53% of the world production in 2012. This species presents high levels of morphological variation that have resulted in different types of varieties not clearly defined. However, morphological characteristics have not been sufficient to classify S. edule varieties, and it has been difficult to study the species genetic diversity. Because of it, there is a need to develop methods for studying the genetic diversity in S. edule. In this regard, the first step is to find a suitable DNA extraction method.… More >

García-Pereyra J¹, GN Aviña-Martínez¹, AA Orozco-Flores², OG Alvarado-Gómez³, M García-Montelongo⁴, G Alejandre-Iturbide⁵, JN Uribe-Soto⁵, H Medrano-Roldan⁶

Phyton-International Journal of Experimental Botany, Vol.83, pp. 37-43, 2014, DOI:10.32604/phyton.2014.83.037

Abstract It was analyzed the genetic variability of (1) three strains of a pathogenic fungi of the genus Metharhizium anisopliae var anisopliae, named MA22, MA24 and MA25, brand spesifik^®; (2) two strains of the product Metasaven^®, named MA1A and MAII; one strain of the product Metatron^®; and a native strain named MACN. This latter strain was directly isolated from the insect chapulin (Brachystola magna), from the farmer, corn plots in Durango, located in north central Mexico. Studies were conducted in the laboratory of molecular biology of the Technological Institute of the Valley of Guardiana in Durango, Mexico, from September 2008 to… More >

Hernández-León R¹, M Martínez-Trujillo², E Valencia-Cantero¹, G Santoyo¹

Phyton-International Journal of Experimental Botany, Vol.81, pp. 133-137, 2012, DOI:10.32604/phyton.2012.81.133

Abstract Rhizospheric soil of wheat plants contains a high diversity of microorganisms, and therefore, comprises a large reservoir for discovering genes with diverse agro-biotechnological applications. In this work, we constructed an E. coli metagenomic library based on bacterial artificial chromosome (BAC) clones with large genomic inserts from metagenomic DNA from the rhizosphere of wheat plants. The average of the DNA cloned segments varies from 5 to 80 kb, with an average size of 38 kb. Random clones were end-sequenced and homology results showed that the clonation of metagenomic DNA codes mainly for metabolic and catalytic functions (40%), including amidohydrolase, hydrolase, peptidase,… More >