Pérez Castañeda LM, MA Cruz Hernández, A Mendoza Herrera

Phyton-International Journal of Experimental Botany, Vol.80, pp. 27-34, 2011, DOI:10.32604/phyton.2011.80.027

Abstract Various studies have shown the symbiotic association of Azospirillum brasilense with different grasses of nutritional importance. A high variability among the isolated strains has been observed. This is consistent with the genetic diversity that Azospirillum has shown in sugarcane isolates. However, if this is also the case in maize and sorghum isolates is unknown. In addition, it has also been reported that most of the isolates come from tropical regions where water is not limiting for maize and sorghum production. We analyzed the genetic variability of Azospirillum brasilense isolates obtained from arid zones of Northeast Mexico in maize and sorghum.… More >

LUCIA PRONSATO, ANABELA LA COLLA, ANA CAROLINA RONDA, LORENA MILANESI, RICARDO BOLAND, ANDREA VASCONSUELO^*

BIOCELL, Vol.37, No.1, pp. 1-9, 2013, DOI:10.32604/biocell.2013.37.001

Abstract Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as lC2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2 O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2 O2 induction of apoptosis. We propose serially cultured C2C12… More >

Tantasawat PA, K Lunwongsa, T Linthaisong, P Wirikitgul, N Campatong, N Talpolkrung, A Tharapreuksapong, O Poolsawat, A Khairum, A Sorntip, C Kativat

Phyton-International Journal of Experimental Botany, Vol.87, pp. 162-170, 2018, DOI:10.32604/phyton.2018.87.162

Abstract Genetic diversity and relatedness of 17 water primrose (Ludwigia hyssopifolia) accessions in Thailand were estimated using morphological characters and random amplified polymorphic DNA (RAPD) markers. Eight morphological characters were diverse among the accessions. However, some accessions could not be distinguished from one another based on these morphological characters alone. Unweighted pair-group arithmetic average (UPGMA) analysis of these characters separated these 17 accessions into 2 major clusters. Among the 5 RAPD primers used, a total of 68 fragments (150 to 2000 bp) were amplified, showing a polymorphism percentage of 80%. The polymorphic information content (PIC) among accessions varied from 0.31 to… More >

Deng JB^1,2*, G Gao³, KS Ahmad⁴, XQ Luo^1,2, FT Zhang^1,2, S Li^1,2, RW Yang⁵

Phyton-International Journal of Experimental Botany, Vol.87, pp. 156-161, 2018, DOI:10.32604/phyton.2018.87.156

Abstract Six mitochondrial (mtDNA) markers, cox2, ccmFn, ccb256, cox3, Nad4L-orf25, and atp4, were combined to assess genetic relationships by using Maximum Likelihood (ML) and Maximum parsimony (MP). This was done among nine Chinese Curcuma herbal species, included two rare species which are difficult to distinguish from their morphological characters. The relationships are very close among the nine species, and the overall distance was 0.018. In this study, the backbone of such nine species was achieved firstly to date, and being divided into two groups with moderate to strong bootstrap support. Both the ML and MP tree were obtained with similarity topologies… More >

Min Yue^1,1, Jeanne C. Stachowiak^1,1,2,2, Arunava Majumdar^1,1,3,3

Molecular & Cellular Biomechanics, Vol.1, No.3, pp. 211-220, 2004, DOI:10.3970/mcb.2004.001.211

Abstract Microchips;ontaining arrays of cantilever beams have been used to mechanically detect and quantitatively analyze multiple reactions of DNA hybridization and antigen-antibody binding simultaneously. The reaction-induced deflection of a cantilever beam reflects the interplay between strain energy increase of the beam and the free energy reduction of a reaction, providing an ideal tool for investigating the connection between mechanics and chemistry of biomolecular reactions. Since free energy reduction is common for all reactions, the cantilever array forms a universal platform for label-free detection of various specific biomolecular reactions. A few such reactions and their implications in biology and biotechnology are discussed. More >

Daxiang Cui¹, Cengiz S. Ozkan², Sathyajith Ravindran³, Yong Kong¹, Huajian Gao¹

Molecular & Cellular Biomechanics, Vol.1, No.2, pp. 113-122, 2004, DOI:10.3970/mcb.2004.001.113

Abstract Experiments on encapsulating Pt--labelled DNA molecules inside multiwalled carbon nanotubes (MWCNT) were performed under temperature and pressure conditions of 400K and 3 Bar. The DNA-CNT hybrids were purified via agarose gel electrophoresis and analyzed via high resolution transmission electron microscopy (HR-TEM) and energy dispersive X-ray spectroscopy (EDX). The results showed that the Pt-labelled DNA molecules attached to the outside walls of CNTs could be removed by electrophoresis. The HR-TEM and EDX results demonstrated that 2-3% of the Pt-labelled DNA molecules were successfully encapsulated inside the MWCNTs. The experimental study complements our previous molecular dynamics simulations on encapsulation of single stranded… More >

Arwa Osama NEMER¹, Mohammad Saud AL ANAZI², Ramesa Shafi BHAT^1*, Arjumand S. WARSY³, Zeneb A BABAY⁴, Mohammad H. ADDAR⁴, Jilani SHAIK², Sooad AL-DAIHAN¹

BIOCELL, Vol.42, No.1, pp. 1-6, 2018, DOI:10.32604/biocell.2018.07005

Abstract Genomic instability and mutations caused by increases in oxidative stress during pregnancy can damage the fetoplacental unit and can upshot preterm birth. Oxidative damage to DNA may possibly be involved in etiology of preterm birth (PTB) which can be repaired by DNA repair gene. In the present study, we assessed the association of base excision repair gene family by analyzing the association of single nucleotide polymorphisms and genes expression in 8-oxoguanine glycosylase-1 (OGG1) and apurinic-apyrimidinic endonuclease 1 (APE1) genes with risk of preterm birth in Saudi women. We analyzed genotypes of four single nucleotide polymorphisms (SNPs) (rs1052133, rs293795, rs2072668 and… More >

Ramzi Bchir^1,2,*, Aslam Sami Djellouli¹, Nadia Zitouna³, Didier Aurelle⁴, Gerard Pergent², Christine Pergent-Martini² and Habib Langar¹

Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 171-184, 2019, DOI:10.32604/phyton.2019.05261

Abstract Specimens of Cymodocea (Viridiplantae, Magnoliophyta) collected on the Tunisian coasts showed a particular morphological and anatomical difference with the classical descriptions of Cymodocea nodosa (Ucria) Asch. the only species of this genus reported in the Mediterranean Sea. In order to precise the taxonomic identity of the new specimens we aimed in this work (i) to verify the identity of the new forms, (ii) to evaluate the genetic diversity of the population, (iii) to test the validity of the existing identification keys of the Tunisian Cymodocea populations. Four stations located in two regions of the Tunisian coasts were sampled. Leaf morphological… More >

Manoochehr MESSRIPOUR¹, Azadeh MESRIPOUR²

BIOCELL, Vol.43, No.1, pp. 37-40, 2019, DOI:10.32604/biocell.2019.05865

Abstract DNA damage and the enzyme poly (ADP-ribose) polymerase (PARP) associated with the pathogenesis of numerous age-related neurodegenerative disorders. Astrocytes play crucial roles in both support metabolic functions and cell viability of the brain. PARP regulates DNA damage and repair in the brain cells. In this study PARP activity and DNA strand break were investigated in the astrocytes isolated from young and aged rat brain. Three and 30-month-old rats were killed by decapitation and brains were removed onto an ice cooled glass plate. Astrocytes were isolated by sucrose density gradient centrifugation and glutamine synthetase (GS) served as a marker of the… More >

S.S. Chowhan¹, G.N. Shinde²

The International Conference on Computational & Experimental Engineering and Sciences, Vol.6, No.1, pp. 1-12, 2008, DOI:10.3970/icces.2008.006.001

Abstract Authentication plays a very critical role in security-related applications like e-commerce. There are a number of methods and techniques for accomplishing this key process. Biometrics is gaining increasing attention in these days. Security systems, having realized the value of biometrics, use biometrics for two basic purposes: to verify or identify users. The use of fingerprints, facial characteristics and other biometrics for identification is becoming more common. This paper overview best of Biometric application for security management. The acquisition of biometric data introduces human research and privacy concerns that must be addressed by the organizations. This paper focus Iris is the… More >