YIFAN LI^1,2, JING ZHANG¹, YITAO FAN¹, HANDAN XIAO¹, KEXIN KANG¹, YALI ZHOU¹, ZHIWEN ZHANG^3,*, YUMIN LI¹, MUZHOU TENG^1,*

BIOCELL, Vol.47, No.8, pp. 1783-1791, 2023, DOI:10.32604/biocell.2023.028056

Abstract Background: ((1-triphenylaminebenzo[c][1,2,5] thiadiazole-4-yl) styryl)-1-methylpyridin methylpyridin-1-ium iodide salt (TBZPy) is a novel photosensitizer that displays excellent photodynamic properties. However, There are few reports on the mechanism of action of the TBZPy photodynamic. Previous studies revealed that photodynamic therapy (PDT) could induce endoplasmic reticulum stress by acting on the endoplasmic reticulum. Therefore, in this study, we investigated the effects of endoplasmic reticulum stress induced by TBZPy-PDT in treating High-risk human papillomavirus (HR-HPV) infection and their underlying mechanisms. Methods: The human cervical cancer cell line HeLa (containing whole genome of HR-HPV18) was treated with TBZPy-PDT. Cell migration, invasion, and colony-forming ability were evaluated… More > Graphic Abstract

XIRU XU^1,#, YUAN FANG^1,#, BIAO ZHANG^1,*, SHICHAO TENG¹, XIANG WU¹, JING ZHANG¹, XIAOQUN GU², MEIXIA MA³

BIOCELL, Vol.47, No.7, pp. 1595-1609, 2023, DOI:10.32604/biocell.2023.027697

Abstract Background: While the Bushen Yizhi Formula can treat Alzheimer’s disease (AD), the yet to be ascertained specific mechanism of action was explored in this work. Methods: Different concentrations of the Bushen Yizhi Formula and amyloid-beta peptide (Aβ) were used to treat rat pheochromocytoma cells (P12) and human neuroblastoma cells (SH-SY5Y). Cell morphological changes were observed to determine the in vitro cell damage. Cell Counting Kit (CCK)-8 assay and flow cytometry were employed to identify cell viability and apoptosis/cell cycle, respectively. Western blotting and immunohistochemistry were employed to measure the expressions of endoplasmic reticulum stress (ERS)-related proteins (GRP78 and CHOP), p-IRE1α,… More >

YONGKANG SHI, JUN MA, KE CHEN, BIN CHEN^*

Oncology Research, Vol.30, No.4, pp. 201-210, 2022, DOI:10.32604/or.2022.027584

Abstract 5-Fluorouracil (5-FU) is a commonly used chemotherapeutic agent for various cancers. However, the drug resistance developed by tumor cells hinders the therapeutic effect. Konjac glucomannan (KGM) is indicated to sensitize 5-FU-resistant hepatocellular carcinoma (HCC) cells to 5-FU. In our study, we found that KGM or 5-FU treatment alone did not affect the malignant cell behaviors and endoplasmic reticulum (ER) stress of 5-FU-resistant HCC cells or HepG2/5-FU and Bel-7402/5-FU cells, while cotreatment with KGM and 5-FU significantly facilitated HCC cell apoptosis and ER stress and suppressed cell proliferation potential and migration abilities. Moreover, we explored the underlying mechanism by which KGM… More >

XINFU FAN^1,2,#, JIE CHEN^3,#, ZONGMENG ZHANG^4,#, FANGHUI CHEN⁵, HAOSEN WANG⁶, YAFEI CAI⁶, JUN LI^7,*

BIOCELL, Vol.46, No.6, pp. 1527-1535, 2022, DOI:10.32604/biocell.2022.018269

Abstract Spinal cord injury (SCI) remains an intractable clinical challenge of neurosurgery, it can be divided into two stages: uncontrollable primary injury induced by mechanical damage and controllable secondary injury regulated by continuous cell death. The apoptosis was the one of most important events in secondary injury, previous studies revealed that excessive endoplasmic reticulum (ER) stress breaks down the homeostasis and triggers apoptosis in the spinal cord. To deter or alleviate the secondary jury, we screen one of fat-soluble compounds, salubrinal, which was an inhibitor of eIF2α dephosphorylation can repair SCI by inhibiting ER stress in mice after SCI. Administration of… More >

XIANGCAI YANG¹, JIAGUI QU², JIEJING LI^1,*

BIOCELL, Vol.46, No.4, pp. 1041-1051, 2022, DOI:10.32604/biocell.2022.017383

Abstract Malignant glioma is one of the most common and deadly tumors in the central nervous system while developing effective treatments for this devastating disease remains a challenge. Previously, we demonstrated that the vitamin nicotinic acid (NA) inhibits glioma invasion. Here, we show that high-dose NA induces apoptosis of malignant glioma cells in vitro and in vivo. In cultured U251 glioma cells treated with NA, we detected ER stress that was likely caused by elevated intracellular calcium levels. The elevated calcium can be attributed to the activation of TRPV1, a cation channel that has been implicated in cutaneous flushing caused by… More >

JINYONG HUANG¹, TIANMING TENG¹, BO BIAN¹, YUCHEN XUE¹, LONGFEI HUANG¹, ZHELONG XU², QING YANG^1,*, YUEMIN SUN^1,*

BIOCELL, Vol.46, No.3, pp. 667-675, 2022, DOI:10.32604/biocell.2021.015917

Abstract Zinc is a trace element involved in maintaining cellular structure and function. Although zinc is associated with left ventricular hypertrophy (LVH), there have been few reports on this association. This study aimed to evaluate the correlation between Zip14 and expression of endoplasmic reticulum stress (ERS) associated molecules in hypertrophied hearts of rats. Dahl salt-sensitive rats were fed a high salt diet to establish a left ventricular hypertrophy (LVH) rat model. RT-PCR was used to determine Zip14, activating transcription factor (ATF4), ATF6, x-box-binding protein 1 (xBP1), C/EBP homologous protein (CHOP), immunoglobulin-binding protein (BiP) mRNA expression. Western blotting was used to evaluate… More >