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  • Open Access

    ARTICLE

    Brief Communication : Spherites in the midgut epithelial cells of the sugarcane borer parasitized by Cotesia flavipes

    DANIELA DE OLIVEIRA PINHEIRO*, HÉLIO CONTE**, ELISA APARECIDA GREGÓRIO*

    BIOCELL, Vol.32, No.1, pp. 61-67, 2008, DOI:10.32604/biocell.2008.32.061

    Abstract Diatraea saccharalis, the main pest of sugarcane, has been controlled by Cotesia flavipes. Very little is known about the effect of parasitism on the host organs, including the midgut. The Lepidoptera midgut epithelium is composed of columnar, goblet, regenerative, and endocrine cells. Spherites have been described in columnar and regenerative cells of several Lepidoptera species, and presented a lot of functional meaning. We identified spherites in the midgut epithelial cells of non-parasitized D. saccharalis larvae analyzed the effect of parasitism on spherite morphology and distribution along the length of the midgut. Midgut fragments of both non-parasitized and parasitized larvae were… More >

  • Open Access

    ARTICLE

    Fine structural analysis of the epithelial cells in the hepatopancreas of Palaemonetes argentinus (Crustacea, Decapoda, Caridea) in intermoult

    LILIANA G. SOUSA1, ELENA I. CUARTAS2, ANA MARÍA PETRIELLA1, 3

    BIOCELL, Vol.29, No.1, pp. 25-31, 2005, DOI:10.32604/biocell.2005.29.025

    Abstract The aim of this study is to describe the ultrastructure of the hepatopancreas of P. argentinus in intermoult. P. argentinus hepatopancreas was studied using standard TEM techniques. Each tubule consists of four cellular types: E (embryonic), F (fibrillar), R (resorptive) and B (blister like). E-cells have embryonic features and some of them were found in mitosis. F, R and B cells possess an apical brush border. F-cells have a central or basal nucleus, a conspicuous RER, and dilated Golgi cisternae. R cells show a polar organization of organelles in three areas: apical, with numerous mitochondria and sER tubules, a central… More >

  • Open Access

    ARTICLE

    Ultrastructure of the ovariole sheath in Diatraea saccharalis (Lepidoptera: Pyralidae)

    DANIELA CARVALHO DOS SANTOS, ELISA APARECIDA GREGÓRIO

    BIOCELL, Vol.26, No.2, pp. 229-235, 2002, DOI:10.32604/biocell.2002.26.229

    Abstract The ultrastructure of the ovariole sheath along the Diatraea saccharalis ovariole was studied by scanning and transmission electron microscopy. Each ovariole is surrounded by an epithelial sheath, a tunica propria and scattered lumen cells. These three components of the ovariole sheath show different ultrastructural features along the ovariole, in the germarium or in the vitellarium; these differences are more evident in the epithelial sheath cells. The epithelial sheath is composed by two layers of cells, the external one running longitudinally and the internal one running circularly in the ovariole. These cells, in vitellarium, present cytoplasmic bundles of myofilaments that are… More >

  • Open Access

    ARTICLE

    Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation

    VANINA L. JULIANELLI1 , LILIANA N. GUERRA*1, JUAN C. CALVO*1,2

    BIOCELL, Vol.31, No.2, pp. 237-245, 2007, DOI:10.32604/biocell.2007.31.237

    Abstract Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis, and differentiation. Mammary gland has being the chosen model for developmental biologist because the most striking changes in morphology and function take place after birth. We have demonstrated a regulation of triglyceride accumulation by protein factors synthesized by normal mouse mammary gland epithelial cells (NMMG), acting on a cell line, 3T3-L1, long used as a model for adipogenesis. In this paper, we demonstrate that this inhibitory effect seems to be shared by other cells of epithelial origin but not by other cell types. We found a regulation of cell proliferation… More >

  • Open Access

    ARTICLE

    Negative Pressure-induced Secretion of Inflammatory Mediators by Cultured Middle Ear Epithelial Cells: Relevance to Eustachian Tube Dysfunction and Otitis Media with Effusion

    P. A. Hebda1, M. R. Barsic1, J. E. Dohar1

    Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 231-232, 2006, DOI:10.32604/mcb.2006.003.231

    Abstract This article has no abstract. More >

  • Open Access

    ARTICLE

    HMGB1 promotes the proliferation and invasion of oral squamous cell carcinoma via activating epithelial-mesenchymal transformation

    Jie REN1, Qian LIANG2

    BIOCELL, Vol.43, No.3, pp. 199-206, 2019, DOI: 10.32604/biocell.2019.07128

    Abstract This study aimed to investigate the role of high mobility group box-1 (HMGB1) expression in oral squamous cell carcinoma; HMGB1 promoted the proliferation and invasion of oral squamous cell carcinoma via activating epithelial-mesenchymal transformation (EMT). In this study, RNA transfection was used to silence the expression of HMGB1 in oral squamous cell carcinoma cells. CCK-8, cell clone formation and trans-well assays were used to detect the proliferation and invasion of cells before and after HMGB1 silencing. qRT-PCR and Western blot were used to detect changes in EMT marker protein expression before and after transfection. HMGB1 was significantly higher in OSCC… More >

  • Open Access

    ARTICLE

    Design and development of a dual-flow bioreactor mimicking intestinal peristalsis and permeability in epithelial tissue barriers for drug transport assessment

    Odin RAMÍREZ-FERNÁNDEZ1,2,3,*, Ludovika CACOPARDO1, Benjamín LEON-MANCILLA2, Joana COSTA1

    BIOCELL, Vol.43, No.1, pp. 29-36, 2019, DOI:10.32604/biocell.2019.04790

    Abstract We present a bioreactor system which combines a semi permeable membrane that simulates the osmotic nutrients interchange in the small intestine circulation and rhythmic peristaltic movement. This custom-designed presents a semipermeable membrane bioreactor, with peristaltic flow and compression variation that allows adjustment of luminal flow rate. In addition, this system is also capable of achieving the drug distribution in the small intestine model from the apical compartment to the basal compartment by the semipermeable channel. This dynamic bioreactor can mimic the human small intestine with increased accuracy to overcome many of the limitations and accuracy with the previously described in… More >

  • Open Access

    ARTICLE

    Biomechanical Aspects of the Auto-digestion Theory

    Geert W. Schmid-Schönbein*

    Molecular & Cellular Biomechanics, Vol.5, No.2, pp. 83-96, 2008, DOI:10.3970/mcb.2008.005.083

    Abstract Increasing evidence suggests that most cardiovascular diseases, tumors and other ailments are associated with an inflammatory cascade. The inflammation is accompanied by activation of cells in the circulation and fundamental changes in the mechanics of the microcirculation, expression of pro-inflammatory genes and downregulation of anti-inflammatory genes, attachment of leukocytes to the endothelium, elevated permeability of the endothelium, and many other events. The evidence has opened great opportunities for medicine to develop new anti-inflammatory interventions. But it also raises a fundamental question: What is the origin of inflammation? I will discuss a basic series of studies that was designed to explore… More >

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