YANG YANG^1,*, BENSHUAI YOU², CHENGLIN ZHOU^1,*

BIOCELL, Vol.46, No.6, pp. 1557-1564, 2022, DOI:10.32604/biocell.2022.018326

Abstract The gene of Helicobacter pylori can encode three to four type IV secretory systems, of which a new gene region has been found in the H. pylori plasticity region. The coding products of this region can form a new T4SS named tfs3, but its function is unclear. This study investigated the effect of VirD4 recombinant protein in the tfs3 secretory system of the H. pylori clinical strain SBK on GES-1 cells. We observed changes in cell morphology after VirD4 treatment. Further analysis indicated that VirD4 increased inflammation by increasing the activation of NF-κB. VirD4 can also inhibited proliferation, and induced… More >

NING LI^1,#, YANG CHEN^2,#, YONGJIE YANG^3,4, SHUHAN LYU¹, YUE PAN^1,5,*

BIOCELL, Vol.46, No.6, pp. 1473-1482, 2022, DOI:10.32604/biocell.2022.018278

Abstract Breast cancer is a highly aggressive cancer in females. Metastasis is a major obstacle to the efficient and successful treatment of breast cancer. Cetyltrimethylammonium bromide (CTAB) has anti-tumor effects on a variety of tumors. We showed that CTAB inhibits the metastasis of breast cancer to the lungs both in vitro and in vivo. Epithelial-mesenchymal transition (EMT) is thought to be one of the major processes mediating breast cancer metastasis. We found that CTAB suppressed EMT and regulated the levels of the classical EMT markers E-cadherin, N-cadherin, vimentin, Snail and Twist1. Moreover, as a candidate anti-tumor agent, CTAB showed primary safety… More >

CLAIRE ELIZABETH HILLS, ELEFTHERIOS SIAMANTOURAS, PAUL EDWARD SQUIRES^*

BIOCELL, Vol.46, No.4, pp. 937-940, 2022, DOI:10.32604/biocell.2022.018414

Abstract Changes in cell-cell and cell-substrate adhesion markers are increasingly used to characterize disease onset and progression. However, these relationships depend on both the biochemical and molecular association between cells and between cells and their extracellular matrix, as well as the biophysical and mechanical properties orchestrated by cytoskeletal, membrane and matrix components. To fully appreciate the role of cell adhesion when determining normal physiology and the impact of disease on cellular function, it is important to consider both biochemical and biophysical attributes of the system being investigated. In this short viewpoint we reflect on our experiences assessing cell-cell and/or cell-matrix interactions… More >

LUCÍA VELOZ^1,2, SANTIAGO A. BOSCH^1,3, GONZALO APARICIO^1,2,*, FLAVIO R. ZOLESSI^1,2,*

BIOCELL, Vol.46, No.3, pp. 639-644, 2022, DOI:10.32604/biocell.2022.018798

Abstract Cell extrusion is an active mechanism to eliminate non-viable or supernumerary cells in healthy epithelia. It also plays a role in carcinogenesis, both in tumor growth (apical extrusion) and metastasis (basal extrusion). Embryonic tissues like the neuroepithelium, on the other hand, present rates of proliferation comparable to that of carcinomas, without the occurrence of cell extrusion. However, the downregulation or phosphorylation of actin-modulating proteins like MARCKS, causes extensive neuroepithelial apical cell extrusion. As changes in MARCKS proteins phosphorylation and expression have also been correlated to carcinogenesis, we propose here an integrated model for their functions in epithelial integrity. More >

SHUTING GU^1,2,#, JINGYI QIN^3,#, SAINAN GAO¹, ZHEN WANG^1,4, QI MENG^1,4, YAN LI^1,4, BING LU⁵, SONGLIN ZHOU^1,2,*, YUNZHAO XU^1,6,*

BIOCELL, Vol.46, No.3, pp. 689-697, 2022, DOI:10.32604/biocell.2022.016225

Abstract Recently, abnormal expression of KIAA1199 has been detected in Epithelial Ovarian Cancer (EOC). However, the underlined anti-ovarian cancer mechanism of KIAA1199 remains to be enlightened. In our study, we performed to elucidate the effects of KIAA1199 on the advanced biological behavior of EOC cells through activation of the IL-6/STAT3 pathway. Confirmed by immunohistochemistry, KIAA1199 was highly expressed in ovarian borderline and malignant epithelial tumors. A retrospective analysis found that EOC patients with low expression of KIAA1199 had a significantly higher 5-year survival rate than those with high expression. Mechanistically, IL-6 was used to stimulate EOC cells, and the expression of… More >

XUE JIANG^1,2, HONG ZHANG^1,2,*

BIOCELL, Vol.46, No.3, pp. 785-794, 2022, DOI:10.32604/biocell.2022.016167

Abstract Posterior capsular opacification (PCO) is linked to the pathological process of lens epithelial cells, which includes proliferation, migration, and epithelial-mesenchymal transition (EMT). Our goal was to investigate whether long noncoding RNA (lncRNA) XIST contributes to EMT via targeting miR-124/Slug axis in TGF-β2-induced SRA01/04 cells. EMT was induced by stimulating SRA01/04 cells with 10 ng/mL TGF-β2 for 24 h, and PCO microenvironment was simulated. The expressions levels of lncRNA XIST, miR-124, and Slug were measured by real-time polymerase chain reaction (RT-PCR) and western blot. The role and mechanism of lncRNA XIST in promoting EMT of TGF-β2-treated SRA01/04 cells were investigated by… More >

JIUCHUN GUO¹, JIE PAN^2,*, QIANQIAN GUO²

BIOCELL, Vol.46, No.2, pp. 505-510, 2022, DOI:10.32604/biocell.2021.015785

Abstract The excessive energy of light, especially the invisible rays with lower wavelength, is basically absorbed by retinal pigment epithelium (RPE) and usually causes DNA damage. The molecular mechanism behind DNA damage repair response to this frequent stress in RPE is not clearly understood. In this study, we determined that the Fanconi anemia (FA) pathway was activated in human RPE ARPE-19 cells after ultraviolet (UV) B and C treatment. Moreover, immunoprecipitation (IP) of FANCD2 indicated that denticleless E3 ubiquitin protein ligase homolog (DTL) closely interacted with FANCD2. Knockdown of DTL weakened the activity of the FA pathway in ARPE-19 cells responding… More >

SICONG WANG¹, RUIJIN LIU², QIUYUAN HAN², KAIJIANG YU^3,*

BIOCELL, Vol.46, No.1, pp. 149-158, 2022, DOI:10.32604/biocell.2021.013027

Abstract Acute kidney injury (AKI), commonly occurring as complications of sepsis, cardiac surgery, and liver or kidney transplantation, is a critical care syndrome. It is well known that lipopolysaccharide (LPS) shock is a common triggering factor for AKI. This study is aimed to examine the effect of flavonoid compound hispidulin on LPS-induced AKI. For this, renal tubular epithelial cell HK-2 was treated with LPS to establish an in vitro model of AKI. The effect of hispidulin on HK-2 cell viability was examined using CCK-8 assay. Cell apoptosis was determined by TUNEL and flow cytometry. Apoptosis marker proteins were determined by using… More >

AJIT C. DHADVE^1,2, PRITHA RAY^1,2

BIOCELL, Vol.46, No.1, pp. 75-86, 2022, DOI:10.32604/biocell.2022.016346

Abstract Progression, relapse, and therapy resistance are the most challenging features of cancer therapy that have been postulated to be driven by Cancer Stem Cell (CSC) population. This enigmatic subpopulation of cancer cells has therefore emerged as promising therapeutic candidate. We earlier reported enrichment of CSC-like side population (SP) with increasing resistance towards Cisplatin and Paclitaxel either alone or in combination in epithelial ovarian cancer (EOC) cells. This SP population is a small proportion of the total population of cancer cells characterised with high expression of drug transporters, a unique feature of stem cells and thereby can be isolated through their… More >

TING LAN, SHENG QIN, XIAOJIN GONG, PING ZHENG, JIAXIN YAN, YANG LIU^*

BIOCELL, Vol.45, No.5, pp. 1313-1319, 2021, DOI:10.32604/biocell.2021.015923

Abstract Invasive stratified mucin-producing carcinoma (ISMC) is a recently described histologic variant of high-risk human papillomavirus (HPV)-associated endocervical adenocarcinoma, as the putative invasive counterpart of the stratified mucin-producing intraepithelial lesion (SMILE). ISMC can display variable architectural patterns and usually coexists with other more conventional types of HPV-associated carcinomas, which makes diagnosis and differential diagnosis of ISMC is difficult for pathologists. Moreover, the prognosis of ISMC is still controversial. We analyzed 6 ISMCs with detailed pathological and clinical information. Intraepithelial lesion, including 1 high-grade squamous intraepithelial lesion and 1 SMILE, was found. Various architectures were observed (including nest, glandular, solid, trabecular, and… More >