FERENC SIPOS^*, GYÖRGYI MŰZES

BIOCELL, Vol.46, No.4, pp. 903-906, 2022, DOI:10.32604/biocell.2022.018200

Abstract Though mesenchymal stem cells (MSCs) are considered as an important pillar of regenerative medicine, their regenerative potential has been shown to be limited in several pathological conditions. The adverse properties of MSC-based cell therapy have drawn attention to the therapeutic use of MSC-derived secretome. However, MSC-originated exosomes and microvesicles can also possess a significant impact on disease development, including cancer. By interchanging secretome, MSCs can interact with tumor cells and promote mutual exchange/induction of cellular markers. In addition, enzymes secreted into and activated within exosomes can result in the acquisition of new tumor cell properties. Therefore, therapeutic applications of MSC-derived… More >

SERGIU PASCA^1,*, ANCUTA JURJ²

BIOCELL, Vol.46, No.3, pp. 579-581, 2022, DOI:10.32604/biocell.2022.017926

Abstract Ten eleven translocation (TET) enzymes are composed of three representatives: TET1/2/3 which are involved in the hydroxymethylation of methylated cytosines. Because of the wide array of processes that are governed by these epigenetic marks, there have been a wide range of clinical effects associated with TET alterations. Even though many research groups have focused on analyzing the effect of TET alterations within certain cells, few have taken into consideration the effect of TET in the context of intercellular communication. One important entity through which intercellular communication occurs is represented by exosomes. Thus, in the current viewpoint we discussed the direct… More >

MINGMING FANG^1,#, NING GE^2,#, JIANFANG LIU^3,*, YAYUN CUI^2,*

BIOCELL, Vol.46, No.3, pp. 711-720, 2022, DOI:10.32604/biocell.2022.016714

Abstract The resistance of cancer cells to the anti-cancer drugs is the most important reason that affecting the efficacy of the non-small cell lung cancer (NSCLC) chemotherapy; thus, to explore the underlying mechanism of drug resistance of NSCLC medications is urgently needed for improving the therapeutic efficacy of current anti-NSCLC chemotherapies. The aim of the present study is to explore the roles of exosomes in the chemosensitivity of A549 cells and the related mechanism. A549 cells and cisplatin resistant cell line A549/DDP derived exosomes were isolated, and the expressions of CXCR4 were compared. Then, after cisplatin treatment, A549 cells were treated… More >

TAILIN WU^1,#, XIANG ZHOU^2,#, CANHUA YE¹, WENCAN LU¹, HAITAO LIN¹, YANZHE WEI¹, ZEKAI KE¹, ZHENGJI HUANG¹, JIANZHOU LUO¹, HUIREN TAO¹, CHUNGUANG DUAN^1,*

BIOCELL, Vol.46, No.2, pp. 495-503, 2022, DOI:10.32604/biocell.2022.015214

Abstract Differentiated macrophages have been proven to participate in the development of mesenchymal stem cells in different tissues. However, the regulatory processes remain obscure. Exosomes, which are key secretions of macrophages, have attracted increasing attention. Therefore, macrophage-derived exosomes may modulate the development of Bone marrow mesenchymal stem cells (BMMSCs). Different culture conditions were used to induce M1 polarization in THP1 cells. Subsequently, exosomes derived from unpolarized (M0) and polarized (M1) macrophages were isolated, BMMSCs were cultured with normal complete medium or inductive medium supplemented with M0 or M1 derived exosomes, and the osteogenic capacity of the BMMSCs was measured and analyzed.… More >

HAIZHENG LIU¹, SHAOFEI CHANG^2,*

BIOCELL, Vol.46, No.2, pp. 453-462, 2022, DOI:10.32604/biocell.2021.015340

Abstract Lysophosphatidylcholine acyltransferase 1 (LPCAT1) is a phospholipid acyltransferase that promotes phospholipid synthesis and plasma membrane reconstruction. Exosomes play an important role in tumor metastasis. The release and uptake of exosomes are key steps of their functions and depend on plasma membrane fusion and plasma membrane receptors, respectively. The purpose of this study was to explore whether LPCAT1-induced plasma membrane remodeling would change the secretion and uptake behavior of exosomes in tumor cells. We first confirmed the abnormally high expression of LPCAT1 in colorectal cancer cells by quantitative real-time PCR (qPCR) and Western blot analysis. Then, SW620 cells were used as… More >

YASHVI SHARMA, SUCHI GUPTA, SUJATA MOHANTY^*

BIOCELL, Vol.45, No.3, pp. 517-520, 2021, DOI:10.32604/biocell.2021.014621

Abstract In these times of despair when a nano-sized organism, the SARS-CoV-2, has rendered the human race helpless, made the global health status decline, and drowned the world economy, a ray of hope comes from another nano-sized particle, the exosome. The potential of mesenchymal stem cells has already been established in COVID-19; however, cell-based therapy has its risks. We thereby propose cell-free therapy using stem cells-derived exosomes to fight against COVID-19, as they can be a game-changer owing to their immunomodulatory nature, which combats the cytokine storm characterizing this disease, and their practical efficiency, which will realistically aid large access to… More >

YUE WANG, YUNFEI ZHENG^*, WEIRAN LI^*

BIOCELL, Vol.45, No.2, pp. 427-444, 2021, DOI:10.32604/biocell.2021.013960

Abstract Orthodontic tooth movement is triggered by orthodontic force loading on the periodontal ligament and is achieved by alveolar bone remodeling, which is regulated by intimate crosstalk between osteoclastogenesis and osteoblast differentiation. Whether the communication between osteoclasts and osteoblasts is influenced by orthodontic compression stress requires further clarification. In this study, osteoclasts were differentiated for 10 days. On day 4 of differentiation, the number of pre-osteoclasts peaked, as determined by the increased expression of RANK and the number of multinucleated cells. After 24 h of compression stress loading, on day 4, the number of osteoclasts increased, and the optimal magnitude of… More >

PENG LIU^1,2, ANFANG ZOU³, QI CHEN⁴, BIAO CHENG^1,*, QIN LI^1,*

BIOCELL, Vol.45, No.1, pp. 27-39, 2021, DOI:10.32604/biocell.2021.012601

Abstract The diabetic ulcer is one of the serious complications of diabetes. In this study, we aimed to establish an exosomal microRNA (miRNA)-targeted messenger RNA (mRNA) regulatory network for screening new biomarkers for diabetic ulcer treatment. For this purpose, exosomes were extracted from bone marrow stem cells (BMSCs) collected from diabetic ulcer patients and healthy adults. The miRNAs in exosomes was detected by high-throughput sequencing analysis. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the differential miRNAs were performed. The miRNA-mRNA regulatory network between candidate miRNAs and their target genes were constructed by… More >

QI SONG¹, JUN ZHANG¹, QIANG ZHANG¹, JING LIU¹, KE LV¹, JIALU YAO^1,2,3,*, YAFENG ZHOU^2,3,*

BIOCELL, Vol.44, No.4, pp. 623-629, 2020, DOI:10.32604/biocell.2020.012645

Abstract Macrophages play an essential role in the myocardial ischemia-reperfusion injury (MIRI), and the macrophage shifting from M1 to M2 phenotypes might be a potential strategy for the treatment of MIRI. It has been reported that miR-182 plays an important role in MSC-Exo-associated macrophage polarization. As circBCRC-3 is a newly discovered circle RNA that worked as a sponge of miR-182, this research aimed to find if circBCRC-3 plays a role in MSC-Exo-associated macrophage polarization. Firstly, circBCRC-3 was identified by divergent primers in mesenchymal stem cells (MSCs). Secondly, the exosome of MSCs was isolated and identified by transmission electron microscopy (TEM), nanoparticle-tracking… More >