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  • Open Access

    ARTICLE

    Germinating seeds of the mung bean, Vigna radiata (Fabaceae), as a model for the preliminary evaluation of cytotoxic effects of drugs

    VIJAY L. KUMAR*, ABHISHEK SINGHAL

    BIOCELL, Vol.33, No.1, pp. 19-24, 2009, DOI:10.32604/biocell.2009.33.019

    Abstract Cytotoxic properties of plant extracts and drugs being developed for cancer treatment are usually evaluated by a variety of in vivo and in vitro tests carried out in animal or plant based models. In the present study we have evaluated the possibility of using the germinating mung beans (Vigna radiata), for rapid and inexpensive screening of drugs exhibiting cytotoxic properties. Mung beans were allowed to germinate either in tap water or in different drug solutions, and parameters like percent germination, increase in radicle length, change in seedling weight and mitotic index of apical root meristems were determined at two time… More >

  • Open Access

    ARTICLE

    Brief Note : Chromosome comparison between populations of the collared peccary, Tayassu tajacu, raised in captivity

    PATRÍCIA CARVALHO DE SOUZA, ANDRÉ SALIM KHAYAT, IGOR CHAMON SELIGMANN, ROMMEL MARIO RODRÍGUEZ BURBANO

    BIOCELL, Vol.32, No.2, pp. 207-210, 2008, DOI:10.32604/biocell.2008.32.207

    Abstract The collared peccary (Tayassu tajacu) is widely distributed over the American continent, being found from the south of the USA to the north of Argentina. In Brazil, it is spread all over the country, being one of the potential species to be raised in captivity. Therefore, the cytogenetic techniques could be a potential tool for reproductive monitoring of animals raised in captivity, mainly when destined for commercial purposes. This study had the objective of determining the chromosome number of two populations raised in captivity and characterizing them by GTG banding. For this purpose, an analysis was made of mitotic metaphases… More >

  • Open Access

    ARTICLE

    Microcutting culture and morpho-physiological changes during acclimation in two Lycium chilense cytotypes

    PABLO HORACIO MASEDA1, JORGE HUGO LEMCOFF1, MERCEDES MURÚA2, NORA FRAYSSINET3, MARTA SUSANA CARCELLER1†

    BIOCELL, Vol.28, No.3, pp. 271-277, 2004, DOI:10.32604/biocell.2004.28.271

    Abstract Lycium chilense, a deciduous perennial shrub, is one of the endangered native species of Patagonia due to sheep overgrazing. Chances of recolonization by seeds are scarce due to the limited density of propagules in the soil and very specific requirements for germination. The objective was to develop an in vitro propagation protocol that would help to perform reestablishment of this species in degraded areas of the Patagonian steppe. Seeds came from two provenances with different somatic number due to differences in ploidy level. Defoliated microcuttings were planted in test tubes with different growing media and taken to a growth chamber.… More >

  • Open Access

    ARTICLE

    Trichoderma spp. fostering growth on Capsicum chinense Jacq. seedlings and antagonistic against Meloidogyne incognita

    Candelero DJ1, AJ Cristóbal1, RA Reyes1, SJM Tun1, AMM Gamboa2, SE Ruíz1

    Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 113-119, 2015, DOI:10.32604/phyton.2015.84.113

    Abstract Fourteen native strains of Trichoderma spp. from wildand agricultural pathosystems in the state of Yucatan, Mexico, with growth-promoting ability of Capsicum chinense Jacq. seedlings were evaluated and antagonistic effect of their filtrate against second-stage juveniles (J2) of Meloidogyne incognita. The strains Th05-02 and Th27-08 showed the best significant effects on plant hight variable increments 55.57 and 47.62%, theTh07-04 with 29.48% more root length, theTh02-01 and Th07-04 isolates increased from 48.71 to 84.61% in volume radical and 53.40% of total dry biomass. Statistical analysis (p≤0.001) of Th43 and Th43-13-14 filtrates caused 100% mortality at 24 and 48h. In the test of… More >

  • Open Access

    ARTICLE

    Sodium sulfate exposure slows growth of native pecan seedlings

    Moreno-Izaguirre E1, D Ojeda-Barrios2, G Avila-Quezada2, V Guerrero-Prieto3, R Parra-Quezada3, T Ruiz-Anchondo2

    Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 80-85, 2015, DOI:10.32604/phyton.2015.84.080

    Abstract Pecan [Carya illinoensis (Wanngenh) K. Koch] is one of the most important nut crops in arid and semiarid regions of Mexico. Here, most pecans are grown in saline soils having poor permeability which are further degraded by the use of low-quality irrigation water. Salinity adversely affects both pecan nut quality and yield. Little work has been done to explore the physiological effects of salinity on native pecan trees. Here we examine physiological changes determined by exposure of pecan seedlings to sodium sulfate (Na2SO4) at four concentrations: 1000, 2000, 3000 and 4000 mg/L applied twice weekly over a 70 d period.… More >

  • Open Access

    ARTICLE

    Variability in the number of stems in Lotus tenuis seedlings growing at high density

    Entio LJ y MM Mujica

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 243-250, 2014, DOI:10.32604/phyton.2014.83.243

    Abstract Successful establishment of Lotus tenuis, an important legume to improve grasslands in the Pampa Deprimida, might be limited by low seedling vigor. The number of crown stems is an important component of vigor. The objective of this work was to determine the variability in the number of crown stems on seedlings of 11 Lotus tenuis natural populations sown at high density under semi-controlled conditions. Seeds of all populations were collected in the Province of Buenos Aires, Argentina. They were sown in plastic pots and cultivated in a greenhouse. Eight weeks after seeding, seedlings of each population were grouped according to… More >

  • Open Access

    ARTICLE

    Anatomophysiological modifications induced by solid agricultural waste (vermicompost) in lettuce seedlings (Lactuca sativa L.)

    Argüello JA, L Seisdedos, MC Díaz Goldfarb, EA Fabio, SB Núñez, A Ledesma

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 289-295, 2013, DOI:10.32604/phyton.2013.82.289

    Abstract The objective of this work was to analyze the impact of a vermicompost treatment on anatomical and physiological modifications related to assimilate partitioning and growth in lettuce seedlings. The results showed that vermicompost increased growth, which was most likely due to an increased activity of the ground meristem of the leaf blade. A greater height and number of chlorenchyma layers were observed in the leaf blade. This was related to an increase in the photosynthetic activity, expressed by an increase in the net assimilation rate. Vermicompost also showed an effect at the procambium level, producing an increase in the number… More >

  • Open Access

    ARTICLE

    Comparison of photosynthetic and morphological characteristics, and microstructure of roots and shoots, between columnar apple and standard apple trees of hybrid seedlings

    Zhang YG, HY Dai

    Phyton-International Journal of Experimental Botany, Vol.80, pp. 119-125, 2011, DOI:10.32604/phyton.2011.80.119

    Abstract The photosynthetic and morphological characteristics, and microstructure of roots and shoots, were studied between columnar apple and standard apple trees derived from the crosses of ‘Fuji’ × ‘Telamon’ and ‘Gala’ × ‘Telamon’. The results showed that chlorophyll A, chlorophyll B, the content of chlorophyll A+B, and leaf area index were higher in columnar apple trees than in standard apple trees. In a day cycle, the net photosynthetic (Pn) and transpiration rates (Tr) were higher in the columnar apple trees than in standard apple trees. The daily variations of Pn and Tr were of a double peak cure. Light saturation points… More >

  • Open Access

    ARTICLE

    Indole-3-butyric acid on rooting and endogenous plant hormones in tetraploid and diploid Robinia pseudoacacia hardwood cuttings

    Wang XL1,2, Z Zhao1, JE Quan1

    Phyton-International Journal of Experimental Botany, Vol.80, pp. 93-100, 2011, DOI:10.32604/phyton.2011.80.093

    Abstract Robinia pseudoacacia (locust hereafter) is an ornamental tree with various uses. Both homologous tetraploid (tetraploid hereafter) and diploid cultivars are commercially available. The tetraploids have advantageous traits, but poor germinability, and cuttings are difficult to root. Since auxin applications can promote rooting, we evaluated the effects of dipping cuttings in various indole-3-butyric acid (IBA) concentrations for four hours on rooting parameters. IBA significantly affected all measured parameters of both tetraploid and diploids cuttings. Tetraploid cuttings produced no roots without IBA. The effects were strongest at 1000 and 800 mg/L IBA for tetraploids and diploids, respectively. Values for tetraploids and diploids… More >

  • Open Access

    ARTICLE

    Identification of axillary buds of potato seedlings based on a vision system with fuzzy logic

    Martínez Corral L1, E Martínez-Rubin2, F F lores-García3, M Vázquez-Rueda3, J Frías-Ramírez2, MA Segura-Castruita2

    Phyton-International Journal of Experimental Botany, Vol.80, pp. 79-84, 2011, DOI:10.32604/phyton.2011.80.079

    Abstract Potato (Solanum tuberosum L.) is a crop whose production yield at national level is very low compared with that in the most productive countries. This is because it is a partially automated crop with deficient and inadequate agronomic practices, low technification levels and great quantity of work wages required per hectare of cultivation. The necessity to generate technical and modern procedures that increase crop production, quality and yield has fostered development of projects leading to obtain seedlings free of pathogens with material of high genetic, physiological and sanitary quality. Utilization of a vision system for the computerized visual recognition of… More >

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