Ocaña-de Jesús RL, AT Gutiérrez-Ibáñez, JR Sánchez-Pale, MD Mariezcurrena-Berasain, G Velázquez-Garduño, A Laguna Cerda, I Rojas Puebla

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 45-50, 2015, DOI:10.32604/phyton.2015.84.045

Abstract The aim of the current research was to determine tomato (Solanum lycopersicum L.) microbiological quality produced under greenhouse conditions in 5 municipalities of the State of Mexico. Studies were conducted during the 2013 production cycle to know the risks and apply prevention strategies prior to its consumption. A microbiological analysis of samples of irrigation water, soil and 100 tomato fruits variety cid was performed to determine Aerobic Mesophiles, Total Coliforms and Fecal Coliforms. The methodology used were those according to the Official Mexican Standards NOM- 109-SSA1-1994, NOM-110-SSA1-1994, NOM-092-SSA1-1994, NOM-113-SSA1-1994, and the Regulations of the National French Organization for Standardization (AFNOR)… More >

Grijalba P¹ y A del C Ridao²

Phyton-International Journal of Experimental Botany, Vol.83, pp. 325-332, 2014, DOI:10.32604/phyton.2014.83.325

Abstract Diaporthe phaseolorum var. caulivora is the major causal agent of stem canker in soybean (CTS) in Argentina. It has appeared mainly in the Southern Pampeana sub-region, but also in warm areas, and it has been observed with different levels of incidence, severity and virulence. The objective of this work was to study the growth rate in vitro and the pathogenicity of isolates from soybean plants at different temperatures. Twenty isolates from Buenos Aires Province were selected. The daily growth rate was measured in nine of them and in two reference isolates in potato dextrose agar (APD), under different light/darkness conditions… More >

Zhu XJ^†, YF Hu^†, X Chen, YH Wang, WP Fang, XH Li

Phyton-International Journal of Experimental Botany, Vol.83, pp. 57-63, 2014, DOI:10.32604/phyton.2014.83.057

Abstract The purpose of this study was to evaluate the antagonistic activity of two endophytic fungal strains, Pseudocercospora kaki and Penicillium sclerotiorum, isolated from the leaves of Camellia sinensis, against the rice blast pathogen Magnaporthe grisea. The inhibitory activity of the two endophytes against M. grisea in dual-culture was compared with that in monoculture. It was confirmed that the broth and its ethyl acetate extract of the dual-culture had a much stronger inhibition activity against M. grisea than the monocultures of P. kaki and P. sclerotiorum. The antagonism index of the broth and ethyl acetate extract from dual-culture to the mycelial… More >

Ruiz SE, AJ Cristóbal, RA Reyes, SJ Tun, RA García, AJ Pacheco

Phyton-International Journal of Experimental Botany, Vol.83, pp. 45-47, 2014, DOI:10.32604/phyton.2014.83.045

Abstract The antagonistic activity of native Bacillus subtilis strains from Yucatán peninsula soils were evaluated on two soilborne pathogens. Bacillus subtilis cbck36 and cbrf24 caused more than 60% inhibition of colony growth in Machophomina phaseolina. Cell-free culture filtrate of B. subtilis cbr24 were active against second- stage juveniles (J2) of Meloidogyne incognita (LC₅₀ 25.8% v/v). More >

Barocio-Ceja NB¹, LF Ceja-Torres^1*, JL Morales-García², HV Silva-Rojas³, R Flores-Magallón¹, S Ochoa-Estradav¹

Phyton-International Journal of Experimental Botany, Vol.82, pp. 15-22, 2013, DOI:10.32604/phyton.2013.82.015

Abstract The objectives of this study were to (1) isolate and identify pathogenic fungi from vegetative material with wilt symptoms in tomato plantations belonging to the Cienega of Chapala, Michoacán, Mexico, and (2) determine the antagonistic capacity of Trichoderma sp. and Aspergillus spp. isolated from chicken-manure vermicompost. Pathogens were isolated by means of a completely randomized sampling in 6 locations; 9 plantations were inspected and 45 plants with symptoms of the disease were selected. Portions of root and stem were disinfected and placed on potato-dextroseagar acidified (PDA). Antagonists isolation was made from a dilution of chicken-manure vermicompost of 1 x 10^-2… More >

O’Connor-Sánchez¹ A, AV Domínguez-May¹, MA Keb-Llanes¹, YJ Peña-Ramírez², VA Herrera-Valencia¹

Phyton-International Journal of Experimental Botany, Vol.79, pp. 25-29, 2010, DOI:10.32604/phyton.2010.79.025

Abstract A reliable protocol for plant regeneration of Mullein Nightshade (Solanum donianum Walp.) was developed from in vitro leaf explants. They were cultured on Murashige and Skoog semisolid medium, supplemented with several combinations of zeatin riboside and 1-naphthaleneacetic acid for shoot regeneration. The formulation that originated most shoots per explant was 8.5 μM zeatin riboside in absence of 1-naphthalene acetic acid. Explants with shoots were transferred to Murashige and Skoog medium, with half the normal salt concentration and without plant growth regulators for elongation. Elongated shoots were individualized, and they rooted readily in half-strength Murashige and Skoog medium without plant growth… More >

Manilal¹ A, S Sujith², J Selvin³, C Shakir⁴, G Seghal Kiran⁵

Phyton-International Journal of Experimental Botany, Vol.78, pp. 161-166, 2009, DOI:10.32604/phyton.2009.78.161

Abstract The antibacterial property of the red algae, Falkenbergia hillebrandii (Born) collected from the southwest coast of India (Indian Ocean) was evaluated against three multidrug resistant human pathogens. Four different solvents: ethyl acetate, dichloromethane, methanol and phosphate buffer saline (PBS) were used with this purpose. Dried samples extracted with methanol showed broadest and highest antimicrobial activity when compared to other solvents. However, PBS extract showed no antibacterial activity. The highly active compounds red alga, F. hillebrandii were fractionated and purified using different chromatographic systems, including reverse phase HPLC and GCMS. The analysis revealed that the most abundant metabolite was the oleic… More >

Mojica-Marín^1* V, HA Luna-Olvera², CF Sandoval-Coronado², B Pereyra-Alférez², LH Morales-Ramos², NA González-Aguilar², CE Hernández-Luna², OG Alvarado-Gomez³

Phyton-International Journal of Experimental Botany, Vol.78, pp. 105-110, 2009, DOI:10.32604/phyton.2009.78.105

Abstract Wide spreading of pepper blight on new plantations and on different production areas in Mexico, and the difficulty in controlling this disease, makes it compulsory to develop new control measures. It is expected that these measures provide new tools for controlling this disease in the context of integrated management strategies. The aim of this investigation was to evaluate and determine the antagonistic potential of 64 strains of Bacillus thuringiensis against Rhizoctonia solani, Phytophthora capsici and Fusarium oxysporum by dual culture assays. Strains of B. thuringiensis came from the International Collection of Entomopatogenic Bacillus from FCBUANL. Results showed that 16 strains… More >

Ávila-Quezada^1* G, E Sánchez¹, E Muñoz¹, LR Martínez², E Villalobos²

Phyton-International Journal of Experimental Botany, Vol.77, pp. 129-136, 2008, DOI:10.32604/phyton.2008.77.129

Abstract We determined the microbiological quality of the most important fruits and vegetables in Chihuahua State (Mexico) to know the potential risks and apply prevention strategies prior to their consumption. Samples of jalapeño, bell and serrano pepper, cantaloupe, saladet and grape tomato, peach and Golden and Starkimson apple and of well, channel, irrigation and drinking water, as well as that for washing in packing were analyzed to determine Salmonella spp., Escherichia coli, total and fecal coliforms, fungi and yeasts. Determinations were made following the methodology indicated by the Mexican regulations (NOM-111-SSA1-1994). Sampling was made in the whole Chihuahua State during the… More >

Hapon MV^1,2, JJ Boiteux^1,2, MA Fernández¹, G Lucero^1,2, MF Silva¹, PH Pizzuolo^1,2

Phyton-International Journal of Experimental Botany, Vol.86, pp. 270-277, 2017, DOI:10.32604/phyton.2017.86.270

Abstract The polyphagous plant pathogen Botrytis cinerea has the ability of growing fast under several conditions, and producing grey moulds in different plant tissues. Because of its adaptive plasticity, resistant strains have made difficult their chemical control. Therefore, new strategies of management and the use of natural compounds could be important control alternatives. The aim of this work was to study the potential value of four Argentine native plant extracts and some of its phenolic compounds as biodrugs on B. cinerea. Aqueous plant extracts of Larrea divaricata, Prosopis strombulifera, Tessaria absinthioides and Schinus molle var. areira, were used. The capability to… More >