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  • Open Access

    ARTICLE

    Short Communication: Agmatine inhibits hypoxia-induced TNF-α release from cultured retinal ganglion cells

    SAMIN HONG, KYOUNGSOO PARK, CHAN YUN KIM, GONG JE SEONG

    BIOCELL, Vol.32, No.2, pp. 201-205, 2008, DOI:10.32604/biocell.2008.32.201

    Abstract The effect of hypoxia on the release of tumor necrosis factor-α (TNF-α) in transformed rat retinal ganglion cells (RGCs) and the effect of agmatine on the hypoxia-induced production of TNF-α in RGCs were evaluated. RGCs were cultured under hypoxic conditions with 5% oxygen, with or without 100 μM agmatine. The expression levels of TNF-α and its receptor-1 (TNF-R1) were investigated by Western blot analysis. After 6 hours of hypoxia, we noted an increase in TNF-α production in RGCs. Agmatine significantly reduced TNF-α level after 12 hours of hypoxic treatment. The expression of TNF-R1 was not affected by the hypoxia or… More >

  • Open Access

    ARTICLE

    Determination of vanadium accumulation in onion root cells (Allium cepa L.) and its correlation with toxicity

    LETTY MARCANO, INGRID CARRUYO, YUSMARY FERNÁNDEZ, XIOMARA MONTIEL, ZAIDA TORREALBA.

    BIOCELL, Vol.30, No.2, pp. 259-267, 2006, DOI:10.32604/biocell.2006.30.259

    Abstract The vanadium is a metal that presents great interest from the toxicological point of view, because of the numerous alterations that can take place in different biological systems. This work evaluated the capacity of vanadium accumulation and its correlation with genotoxic effects in root cells of Allium cepa L. The bulbs were cultivated in renovated filtered water each 24 h, at a temperature of 25 ± 0.5°C, in darkness and constant aeration. Treatments were carried out under the same experimental conditions, using water solutions of vanadium of 25, 50, 75 and 100μg/g for 0, 12, 24, 48 and 72 h.… More >

  • Open Access

    ARTICLE

    Apigenin inhibits cell migration through MAPK pathways in human bladder smooth muscle cells

    QINGXIN LIU , XIANGGUI CHEN, GUOLIN YANG1 , XUEWEN MIN3 , AND MAOXIAN DENG1,*

    BIOCELL, Vol.35, No.3, pp. 71-80, 2011, DOI:10.32604/biocell.2011.35.071

    Abstract Apigenin, a nonmutagenic flavonoid, has been shown to possess free radical scavenging activities, anticarcinogenic properties, antioxidant and anti-inflammatory effects. Recently, apigenin was reported to cause gastric relaxation in murine. To assess possible effects of apigenin on migration of bladder smooth muscle (SM) cell, we isolated SM cells from peri-cancer tissue of human bladder and established a cell model that was capable to overexpress transiently MEKK1 (MEK kinase 1). Results showed that overexpression of active human MEKK1 by adenoviruses infection induced migration of human bladder smooth muscle (hBSM) cells and phosphorylation of MAPKs, ERK, JNK and p38, which are the downstream… More >

  • Open Access

    ARTICLE

    Early testing of S1 lines of maize

    Salinas Jiménez V, JC Raya Pérez, CL Aguirre Mancilla, F Chablé Moreno, JG Ramírez Pimentel, G García Rodríguez, J Covarrubias Prieto

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 203-209, 2016, DOI:10.32604/phyton.2016.85.203

    Abstract Eighty S1 maize (Zea mays L.) lines derived from halfsib families with good yield potential (greater than landraces in the region) were evaluated, with the purpose of selecting outstanding lines. Native populations are a source of germplasm for genetic improvement through selection and hybridization. Analysis of variance showed highly significant differences between the lines S1 in the variables days to anthesis (FM), silking date (FF), plant height (Apl), ear height (Amz), leaves above ear (NHEmz), ear length (Lmz), row number (Nhil), kernels per row (Ghil), total kernels per ear (TGmz) and grain yield (Rend). All variables showed high variability, an… More >

  • Open Access

    ARTICLE

    The uptake of cadmium by Allium cepa var. agrogarum L. and its effects on chromosome and nucleolar behavior in root tip cells

    Wang QL, DH Liu, JY Yue

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 155-161, 2016, DOI:10.32604/phyton.2016.85.155

    Abstract Allium cepa var. agrogarum L. seedlings are sensitive to Cd stress. We used fluorescence imaging to indicate that Cd2+ was localized in cytoplasm in the epidermis of the basal parts of root and vascular tissues after Cd treatment. The nucleoli and the cell walls were the first storage sites of Cd2+. When Cd exposure was prolonged, severe irregularly-shaped nuclei were induced. We used silver nitrate staining to analyze the effects of different concentrations (1–300 μM) of cadmium chloride on chromosome, nucleolus and nucleolus organizer regions (NORs) in root tip cells. Cd2+ induced c-mitosis, chromosome bridges, chromosome stickiness and micronuclei. More… More >

  • Open Access

    ARTICLE

    Wereke root (Ibervillea sonorae Greene) descriptive characteristics and biochemical generalities of its aqueous extract

    Sinagawa-García SR1, A Gutiérrez-Díez1, A Mora-Olivo2, MC Juárez-Aragón2, JA Torres-Castillo2

    Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 358-367, 2015, DOI:10.32604/phyton.2015.84.358

    Abstract A general biochemical description of an aqueous extract obtained from Ibervillea sonorae root was reported; furthermore, a histological description for the root is included. A bromatological analysis was carried out, and it indicated that the study nutritional variables were similar to those reported in other edible tuberous roots including sweet potatoe, mandioca, bean yam and arracacha. For the first time, the presence of an active proteinase at alkaline pH and the inhibitory activity of trypsin were reported for this root. Several medical and bioactive properties have been attributed to the wereke root. Nevertheless, deeper studies are needed to determinate the… More >

  • Open Access

    ARTICLE

    First morphogenetic identification of Fusarium solani isolated from orange fruit in Egypt

    Abd-Elsalam KA1,2, K Youssef1,2, H Almoammar3

    Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 128-131, 2015, DOI:10.32604/phyton.2015.84.128

    Abstract Losses due to postharvest decay may occur at any time during postharvest handling, from harvest to consumption affecting the produce quality and quantity. Accurate identification of the pathogen causing postharvest disease is essential to the selection of an appropriate disease control approach. Nine isolates of Fusarium recovered from orange fruit were identified as Fusarium solani. The fungus is involved with fruit decay. The obtained cultures were purified and grown on potato-dextrose agar (PDA), malt yeast agar (MYA), and Czapek's nutrient media (CNM) under light for identification. A pathogenicity test was carried out to fulfil Koch's postulates. The pathogen could only… More >

  • Open Access

    ARTICLE

    Morphoanatomical functional traits in xerophytic species of a saline environment

    Pérez Cuadra V, V Cambi

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 389-396, 2014, DOI:10.32604/phyton.2014.83.389

    Abstract The halophytic community of Salitral de la Vidriera (Buenos Aires Province, Argentina) has species with different morphoanatomical functional traits. The aim of this study was to compare these traits in four species, two Asteraceae (Baccharis spartioides and B. tenella) and two Frankeniaceae (Frankenia juniperoides and F. pulverulenta). Leaves and stems were treated under traditional techniques for anatomical study. Leaves the of Asteraceae and F. pulverulenta were amphistomatic while in F. juniperoides they were hypostomatic. All species showed trichomes and only the Frankeniaceae had salt glands. The mesophyll was isolateral in Asteraceae, and dorsiventral in Frankeniaceae; the number of foliar vascular… More >

  • Open Access

    ARTICLE

    Linking relative growth rates to biomass allocation: the responses of the grass Leymus chinensis to nitrogen addition

    Li1,2 YY, X-T Lü1, Z-W Wang1, C Zhou3,4, X-G Han1

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 283-289, 2014, DOI:10.32604/phyton.2014.83.283

    Abstract Relative growth rate (RGR) of plants is a key component of fitness. Theoretically, the RGR of plants would be closely related with biomass allocation. Our mechanistic understanding of the relationship between RGR and biomass allocation under global change scenarios is still limited. We examined the responses of RGR and biomass allocation of Leymus chinensis, a dominant grass in the temperate steppe of northern China, to a wide range of N addition. We found that N addition increased RGR of L. chinensis up to a threshold of 10 g N/m2. While leaf and stem weight ratios were positively correlated with N… More >

  • Open Access

    ARTICLE

    Water stress response of Tricepiro and its progenitors Triticale and Trigopiro

    Ruiz MA1,3, A Golberg1,2, ML Molas1

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 263-274, 2014, DOI:10.32604/phyton.2014.83.263

    Abstract In semiarid regions rainfall is often scarce and erratic in space and time, making valuable the exploration of news genotypes with superior behavior in dry land systems. Tricepiro (X Triticosecale Wittmack x X Agrotriticum Ciferri & Giacom) is a synthetic cereal with long cycle and high biomass production in semiarid environments. However its performance under controlled water stress has not yet been evaluated. The aim of this work was to investigate the effect of water stress on tricepiro genotypes in comparison with their progenitors: triticale and trigopiro. Morphophysiological traits related to forage yield were evaluated in the greenhouse and the… More >

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