A. A. Román-Calzoncit¹, F. Ramírez-Godina^2,*, J. Sánchez-Laureano², A. C. Flores-Gallegos¹, J. Espinoza-Velázquez², R. Rodríguez-Herrera^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 87-97, 2021, DOI:10.32604/phyton.2020.013880

Abstract Polyembryony in maize (PEm) contributes to improving the nutritional properties of the grain, as well as an increase in yield, since it generates multiple plants per seed, opening the possibility of developing new varieties. However, it is unknown whether polyembryony in maize is the product of chromosomal abnormalities. Based on the above, in this research a cytogenetic study was proposed to verify if chromosomal abnormalities are related to the maize polyembryony. For a meiotic study, maize genotypes with variable proportions of polyembryony (PEm), from the UA-IMM-BAP population and non-PEm (monoembryonic) maize were used, while for a mitosis analysis, 30 families… More >

Sen Wang¹, Fei Zheng¹, Meijing Zhang¹, Jun Tu², Yanping Chen^1,3, Jianhua Yuan^1,3, Qingchang Meng^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 861-871, 2020, DOI:10.32604/phyton.2020.011762

Abstract Endosperm mutants are critical to the studies on both starch synthesis and metabolism and genetic improvement of starch quality in maize. In the present study, a novel maize endosperm mutant A0178 of natural variation was used as the experimental material and identified and then characterized. Through phenotypic identification, genetic analysis, main ingredients measurement and embryo rescue, development of genetic mapping population from A0178, the endosperm mutant gene was located. The results showed that the mutant exhibited extremely low germination ability as attributed to the inhibited embryo development, and amounts of sugars were accumulated in the mutant seeds and more sugars… More >

Cansu Altuntaş¹, Rabiye Terzi^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 487-495, 2020, DOI:10.32604/phyton.2020.08919

Abstract The endoplasmic reticulum (ER) is an organelle in the cell where proteins are created and folded. Folding is a very elaborate process that is often interrupted by various biotic and abiotic stresses, leading to the formation of unfolded and misfolded proteins called ER stress. Dithiothreitol (DTT)-induced unfolded protein response (UPR) in endoplasmic reticulum (ER) has been recently reported in plants. Also, previous studies demonstrated that treatment with polyethylene glycol (PEG₆₀₀₀) could stimulate water deficit in crops. However, further researches should be conducted to elucidate the molecular mechanism of ER stress response and the relationship between water deficiency and ER. In… More >

Alhan Sariyev¹, Celaleddin Barutcular^2,*, Mert Acar¹, Akbar Hossain³, Ayman EL Sabagh^2,4,5,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 519-528, 2020, DOI:10.32604/phyton.2020.09142

Abstract Maize being sub-tropical crop is sensitive to water deficit during the early growth stages; particularly clay-rich soil, due to the compaction of the soil. It is well-documented that potential sub-surface drip irrigation (SDI) (Full irrigation; SDIFull (100% field capacity (FC)), Deficit irrigation; SDIDeficit (70% FC)) improves water use efficiency, which leads to increased crop productivity; since it has a constraint that SDI excludes soil air around the root-zone during irrigation events, which alter the root function and crop performance. Additionally, in clayrich soils, the root system of plants generally suffers the limitation of oxygen, particularly the temporal hypoxia, and occasionally… More >

Peng Jiao^1,#, Ruiqi Ma^2,#, Zhuo Qi¹, Zhenzhong Jiang¹, Siyan Liu¹, Jing Qu³, Shuyan Guan^1,*, Yiyong Ma^3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 121-130, 2020, DOI:10.32604/phyton.2020.07980

Abstract This research uses the immature embryos of inbred maize lines (GSH9901, Hi01, Hi02, and Chang 7-2) as receptor materials to establish the callus induction system. These inbred lines provide the receptor materials for the genetic regeneration of maize and the verification of the genetic functions of maize. The factor experiment and orthogonal experiments were used to investigate the impacts of different genotypes, immature embryo size, shield orientation, 2, 4-D concentration, proline concentration, and folic acid concentration on the induction rate of embryogenic callus tissue. A sensitivity experiment testing glyphosate (Bar) and an antibiotic (Cefotaxime sodium) were also conducted. The results… More >

Marina Tsvetova¹, Lev Elkonin^1,*, Yulia Italianskaya¹

Phyton-International Journal of Experimental Botany, Vol.88, No.4, pp. 389-401, 2019, DOI:10.32604/phyton.2019.07485

Abstract Apomictic seed development is a complex process including formation of unreduced embryo sac, parthenogenetic embryo development from the egg cell, and endosperm formation either autonomously, or due to fertilization of polar nuclei by the sperm (under pseudogamous form of apomixis). In the latter case, an obstacle to the normal endosperm development is disturbance of maternal (m) -to-paternal (p) genomic ratio 2m: 1p that occurs in the cases of pollination of unreduced embryo sac with haploid sperms. Usage of tetraploid pollinators can overcome this problem because in such crosses maternal-to-paternal genomic ratio is 4m: 2p that provides formation of kernels with… More >

A. Sattar^1,*, A. Sher^1,*, M. Ijaz¹, M. Irfan², M. Butt¹, T. Abbas¹, S. Hussain², A. Abbas¹, M. S. Ullah³, M. A. Cheema⁴

Phyton-International Journal of Experimental Botany, Vol.88, No.4, pp. 379-388, 2019, DOI:10.32604/phyton.2019.04784

Abstract Application of biochar to agricultural soils is mostly used to improve soil fertility. Experimental treatments were comprised of two factors: i) drought at two level, i.e., 80% and 40% water holding capacity (WHC) which was maintained on gravimetric basis ii) three levels of biochar i.e., control, 2 t ha^-1 and 4 t ha^-1 added to soil. Experimentation was done to examine potential of biochar application to enhance the growth attributes, water relations, photosynthetic pigments and antioxidants activities in maize (Zea mays L.) seedlings. Results of study revealed that biochar application increased the growth qualities (total seedlings biomass, dry weight of… More >

CLAUDIA NOME^1*, PAULO CÉSAR MAGALHÃES², ELIZABETH OLIVEIRA², SERGIO NOME, IRMA GRACIELA LAGUNA¹

BIOCELL, Vol.33, No.2, pp. 133-136, 2009, DOI:10.32604/biocell.2009.33.133

Abstract Maize plants infected with Spiroplasma kunkelii show symptoms similar to that of plants in a magnesium-deficient soil, and it has been shown that the spiroplasma alters the plants’ magnesium absorption. In the current study we compared changes associated to either spiroplasma infection, two soil magnesium levels and their combinations. Plant symptoms were recorded and correlated with transmission electron microscopy observations. Plants grown on a high magnesium treatment showed no macroscopical alterations nor organelle ultrastructural alterations, while plants on a low magnesium treatment showed macroscopical vein yellowing and, ultrastructurally, they had most chloroplasts and mitochondrial membranes altered. Infected plants on a… More >

Vital-López L, MA Cruz-Hernández, S Fernández-Dávila, A Mendoza-Herrera

Phyton-International Journal of Experimental Botany, Vol.85, pp. 210-217, 2016, DOI:10.32604/phyton.2016.85.210

Abstract Genetically modified crops could cause negative effects on bacterial communities. In this study, we compared the bacterial community structure of two maize cultivars to determine whether the transgenic cultivar exerts a negative effect on bacterial communities inhabiting the rhizosphere. Cultivars included the genetically modified maize (Zea mays), with the pat-gene conferring resistance to the herbicide glufosinate (synonym: L-phosphinothricin), and the hybrid, conventional maize. Metagenomic DNA was extracted from the rhizosphere of plants grown in a greenhouse. Single-strand conformation polymorphism, based on polymerase chain reaction amplifying a partial subunit rRNA gene was used to characterize and generate genetic profiles that corresponded… More >

Salinas Jiménez V, JC Raya Pérez, CL Aguirre Mancilla, F Chablé Moreno, JG Ramírez Pimentel, G García Rodríguez, J Covarrubias Prieto

Phyton-International Journal of Experimental Botany, Vol.85, pp. 203-209, 2016, DOI:10.32604/phyton.2016.85.203

Abstract Eighty S1 maize (Zea mays L.) lines derived from halfsib families with good yield potential (greater than landraces in the region) were evaluated, with the purpose of selecting outstanding lines. Native populations are a source of germplasm for genetic improvement through selection and hybridization. Analysis of variance showed highly significant differences between the lines S1 in the variables days to anthesis (FM), silking date (FF), plant height (Apl), ear height (Amz), leaves above ear (NHEmz), ear length (Lmz), row number (Nhil), kernels per row (Ghil), total kernels per ear (TGmz) and grain yield (Rend). All variables showed high variability, an… More >