Romero-Castillo PA¹, MC Pérez Amador Barron¹, P Guevara Fefer¹, V Muñoz Ocotero¹, A Reyes Dorantes², F Aguirre Garcia², A Amaya Chavez³

Phyton-International Journal of Experimental Botany, Vol.82, pp. 75-80, 2013, DOI:10.32604/phyton.2013.82.075

Abstract The search for new anti-inflammatory drugs with fewer side effects than those currently available in the market has recently attracted the interest of scientists. Medicinal plants might be a natural source of medicines. Such is the case with Ziziphus amole (Sessé & Moc.) M.C. Johnst. or corongoro, a plant species native to México. It is used in traditional medicine as an anti-inflammatory drug, a healing substance and an analgesic, and in the treatment of diarrhea, diabetes, asthma and hemorrhoids. The aim of this study was to evaluate the anti-inflammatory effect of Z. amole in the acute 12-O-tetradecanoylphorbol-13-acetate (TPA) induced mouse… More >

Cerna¹ E, Y Ochoa², R Mendoza¹, MH Badii³, G Gallegos¹, J Landeros¹

Phyton-International Journal of Experimental Botany, Vol.79, pp. 147-152, 2010, DOI:10.32604/phyton.2010.79.147

Abstract Protein assays were conducted on Tetranychus urticae Koch, as potential resistance detection tools to plaguicides. This is a phytophagous mite that feeds on a large variety of plants. Experiments were carried out using a pesticide susceptible and three field crop strains of T. urticae. Protein was measured by colorimetric assays, using Kit-II from Bio-Rad, with bovine serum albumin (BSA) as standard. Homogenates were prepared using 10, 30, 50, 100, 300, 500 and 800 mites, with 30 replicates each. Linearity was obtained for the standard curve of the different methods, and r² values ranged from 0.877 to 0.985. The Bradford method… More >

Pérez-Amador¹ MC, V Muñoz Ocotero¹, F García Jiménez²

Phyton-International Journal of Experimental Botany, Vol.77, pp. 103-111, 2008, DOI:10.32604/phyton.2008.77.103

Abstract Phototoxic compounds (polyacetylenes and thiophenes) and their biological activity were determined on Bidens squarrosa plants by measuring their antibacterial and anti-inflammatory potential. Phototoxic compounds were extracted with hexane, characterized by TLC, and their presence confirmed by U.V. spectrum of their extracts and bactericidal potential. Antibacterial activity of the hexane, ethyl acetate and methanol extracts was measured against Bacillus subtilis and Escherichia coli, and their antiinflammatory potential was determined by the mouse ear edema test. More >

Pérez-Amador¹ MC, V Muñoz Ocotero¹, F García Jiménez²

Phyton-International Journal of Experimental Botany, Vol.77, pp. 21-29, 2008, DOI:10.32604/phyton.2008.77.021

Abstract Leaves of Eupatorium morifolium Mill. were analyzed for phototoxic compounds, and the toxicity of their hexane extract was evaluated against Bacillus subtilis. The phototoxic compounds were analyzed by TLC. In the plate, pale blue spots indicated the presence of these compounds. This was confirmed by the UV extract spectrum and the antibacterial activity against Bacillus subtilis. The biological activity of the leaf extracts (hexane, ethyl acetate or methanol) was also studied. The antiinflammatory activity was determined using the edema test on mouse ears, and the bactericide activity was assayed against Bacillus subtilis and Escherichia coli. A strong anti-inflammatory activity was… More >

Galatro Andrea, Ivan Rousseau, Susana Puntarulo

Phyton-International Journal of Experimental Botany, Vol.75, pp. 7-20, 2006, DOI:10.32604/phyton.2006.75.007

Abstract Electron Paramagnetic Resonance (EPR) has been developed as a versatile field that uses different techniques sharing the common feature of resonant micro-wave radiation absorption by paramagnetic substances. Certain free radicals, such as ascorbyl radical (A•), show an stable spectrum, and can be directly detected by EPR at room temperature. Studies included in this work clearly show that this highly sensitive methodology can be successfully applied to biological systems. Exposure of plants to UV-B radiation was studied as a factor of oxidative stress in plants, and the effect on A^• steady state concentration was analyzed. A kinetic analysis was performed considering… More >

Gabriela MALANGA, Paula Mariela GONZÁLEZ, Juan Manuel OSTERA, Susana PUNTARULO^*

BIOCELL, Vol.40, No.1, pp. 35-38, 2016, DOI:10.32604/biocell.2016.40.035

Abstract The harmful effects of the reactive species may be due to the increase in their steady state concentration either by the enhancement of their production rates and/or the decrease of their consumption rate by antioxidant activity. The ascorbyl radical (A^• ) can be considered as a final product of radical oxidative transformations of ascorbate (AH^-). The ratio A^• content/AH^- content (A^• /AH^-) has been widely used as an interesting tool to estimate mild to moderate oxidative transformations, providing a quick and simple method of diagnosis of stress in the hydrophilic cellular medium. The aim of this work was to summarize… More >

Krupa-Małkiewicz M¹, B Smolik², M Sędzik²

Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 15-23, 2019, DOI:10.32604/phyton.2019.04670

Abstract Salinity is one of the abiotic stresses that limits the growth and productivity of many crops. A possible survival strategy for plant under saline conditions is to use compounds that could minimize the harmful effects of salt stress on the plant development. The objective of the presented study was to investigate the effect of exogenous ascorbic acid (ASA) with or without gibberellic acid (GA3) on key growth and biochemical parameters in two petunia cultivars ‘Prism Rose’ and ‘Prism White’ under saline (150 mM NaCl) and non-saline in vitro condition. Nodal cutting with an axillary buds were used as explants. Application… More >

Yang Cheng, Bin Jia, Ming Xin

The International Conference on Computational & Experimental Engineering and Sciences, Vol.16, No.2, pp. 33-34, 2011, DOI:10.3970/icces.2011.016.033

Abstract A sparse grid approach to orbit uncertainty propagation is presented. Efficient and accurate uncertainty propagation methods for nonlinear dynamic systems have been of enormous interest to space object tracking. Recent methods include those based on the time evolution of the probability density function, the statistical moments, the random samples, or a sum of Gaussian components. The idea of the sparse grid method for orbit uncertainty propagation is to represent the initial uncertainty by a sparse grid, propagate the sparse grid points individually through the nonlinear orbit dynamics, and compute the statistical moments from the propagated sparse grid points. The Smolyak… More >

Shigenori Tanaka¹

The International Conference on Computational & Experimental Engineering and Sciences, Vol.13, No.3, pp. 61-62, 2009, DOI:10.3970/icces.2009.013.061

Abstract Recent developments in ab initio calculations for biomolecular systems such as proteins and nucleic acids are illustrated on the basis of the fragment molecular orbital (FMO) method. Examples of the calculated systems include nuclear receptors with small ligands, cAMP receptor protein complexed with DNA, influenza virus hemagglutinin complexes, and bioluminescent oxyluciferin-luciferase complex. Quantitative calculations with the inclusion of relevant electron correlation effects have well reproduced those experimental results concerning the binding affinity, the mutation effects, the emission spectra, and so on. Feasibility of massively parallel computations with the FMO method is also discussed. More >

J. António¹ , A. Tadeu¹, L. Godinho

The International Conference on Computational & Experimental Engineering and Sciences, Vol.3, No.3, pp. 157-162, 2007, DOI:10.3970/icces.2007.003.157

Abstract A frequency dependent formulation based on the Method of Fundamental Solutions (MFS) is used to simulate the sound wave propagation in a 3D acoustic space. This solution is approximated by a linear combination of fundamental solutions generated by virtual sources placed outside the domain in order to avoid singularities. The coating materials can be assumed to be absorbent. This is achieved in the model prescribing the impedance that is defined as a function of the absorption coefficient. The model is first verified against analytical solutions, provided by the image source technique for a parallelepiped room bounded by rigid walls. The… More >