Shugufta Parveen¹, Fayaz A. Mohiddin^2,*, M. Ashraf Bhat³, Zahoor Ahmed Baba⁴, Fehim Jeelani⁵, M. Anwar Bhat⁶, Sajad Un Nabi⁷, Burhan Hamid², Saba Bandey⁸, Farhanaz Rasool⁹, Zakir Amin¹, Ibrahim Al-Ashkar^10,*, Muhammad Adnan¹¹, Ayman El Sabagh¹²

Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3021-3041, 2023, DOI:10.32604/phyton.2023.030921

Abstract One hundred twenty-five endophytic microorganisms were isolated from the roots, stems, and leaves of four prominent rice cultivars growing in temperate regions. Their potential to combat rice blast disease and promote plant growth was investigated. The dual culture tests highlighted the strong antagonistic activity of five fungal (ranging from 89%–70%) and five bacterial (72%–61%) endophytes. Subsequent examination focused on volatile compounds produced by selected isolates to counter the blast pathogen. Among these, the highest chitinase (13.76 µg mL⁻¹) and siderophore (56.64%), was exhibited by Aspergillus flavus, and the highest HCN production was shown by Stenotrophomonas maltophilia (36.15 µM mL⁻¹). In… More >

Lulu Zhi, Xiangyang Hu^*

Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2825-2840, 2023, DOI:10.32604/phyton.2023.030912

Abstract Plant regeneration is a self-repair of the plant body in response to adverse conditions or damaged structures, and root regeneration allows the plant body to better adapt to its environment by supplementing the roots’ structure. Previous research has shown that adventitious roots can be made to occur from scratch in two ways. Studies that simulate adventitious root regeneration through natural conditions allow the regeneration process to be broadly divided into three stages: the perception of early signals, the massive accumulation of auxin, and the transformation of cell fate. The strength of regeneration, in turn, is influenced by wounding, stress, hormones,… More >

Ali Bandehagh^1,*, Zahra Dehghanian², Vahideh Gougerdchi¹, Mohammad Anwar Hossain^3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2301-2324, 2023, DOI:10.32604/phyton.2023.028586

Abstract The presence of selenium (Se) is not widely established as crucial for crops, although it is commonly recognized as an important nutrient for animals as well as humans. Even so, it is inevitably accepted that Se usually contributes positively to the life cycle of plants. Previous findings suggested that small amounts of Se seem to have a productive role in growth and production. As a result, Se is assumed to function in multiple ways, primarily by influencing a variety of biochemical and physiological functions. Also, Se also acts as a plant antioxidant and pro-oxidant and confers tolerance against different abiotic… More >

Lingyan Hu, Haiyan Chen, Guangqun Zhang, Zihao Yu, Kai Yan, Fangdong Zhan, Yongmei He^*

Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1933-1944, 2022, DOI:10.32604/phyton.2022.020041

Abstract Indoleacetic acid (IAA) is an important regulator that plays a crucial role in plant growth and responses to abiotic stresses. In the present study, a sand cultivation experiment was carried out to investigate the effects of IAA at different concentrations (0, 0.01, 0.1, 0.5, 1, and 2.5 mmol/L) on maize growth, root morphology, mineral elements (Ca, Mg) and Cd uptake under 20 mg/kg Cd stress. The results showed that 0.01 mmol/L is the optimal IAA concentration for enhancing the Cd tolerance of maize. Compared with the control treatment, 0.01 mmol/L IAA promoted maize growth, with significant increases in the height,… More >

Longhua Zhou^1,2,#, Ting He^1,2,#, Jing Li^1,2,3, Guimei Guo^1,2, Yingbo Li^1,2, Hongwei Xu^1,2, Runhong Gao^1,2, Linli Huang^1,2, Yifei Wang^1,2, Ruiju Lu^1,2, Zhiwei Chen^1,2,*, Chenghong Liu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1419-1428, 2022, DOI:10.32604/phyton.2022.019912

Abstract Phytohormones play important roles in plant growth and development, and polyploids are thought to be an important method for plant breeding. However, the relationship between ploidy and phytohormone is still unclear. In this study, barley at three ploidy levels were produced by microspore culture. Therefore, we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and distribution. The results showed that Abscisic acid (ABA), gibberellin (GA), jasmonic acid (JA), auxin (IAA), salicylic acid (SA) and cytokinin (CTK) were successfully determined in shoots and… More >

XIAOFEN CHENG¹, ABEER HASHEM^2,3, ELSAYED FATHI ABD_ALLAH⁴, QIANGSHENG WU^1,5,*, KAMIL KUČA^5,*

BIOCELL, Vol.45, No.6, pp. 1687-1694, 2021, DOI:10.32604/biocell.2021.017581

Abstract Gibberellins (GAs) are an important hormone in regulating plant growth and development, and DELLA protein is an essential negative regulator of GA signal transduction. The aim of the study was to clone a GA-inhibiting protein DELLA from trifoliate orange (Poncirus trifoliata L. Raf.) and to analyze the bioinformations and expression patterns of the protein gene in tissues and in response to drought stress. A DELLA protein was isolated from trifoliate orange and named as PtGai (Genebank number: MZ170959). The PtGai protein had 1731 bp open reading frames, along with 576 amino acid codes, and also grouped with sweet orange (XM_006430552.4).… More >

Hans Christian Correa-Aguado^1,3, Gloria Viviana Cerrillo-Rojas¹, Alejandro Rocha-Uribe², Ruth Elena Soria-Guerra², José Francisco Morales-Domínguez^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 515-531, 2021, DOI:10.32604/phyton.2021.013619

Abstract The need for renewable energy sources makes microalgae an essential feedstock for biofuels production. The molecular aspects and the response to nitrogen (N)-limited conditions with a phytohormone stimulus in microalgae have been slightly explored. In this work, Scenedesmus obliquus was used as a study model to analyze the effect of benzyl amino purine (BAP) and gibberellic acid (GA) coupled to nitrogen limitation on cell growth, biomass and fatty acids. The selected 10^-5 M BAP increased the biomass by 1.44-fold, and 10^-6 M GA by 1.35-fold. The total lipids also increased by 2.8 and 1.11-fold, respectively. The 10^-5 M BAP and… More >

BEEMNET MENGESHA KASSAHUN^1,#, BEUM-CHANG KANG^2,#, SU-JI BAE², YE JIN NAM¹, GRETEL FONSECA MUNDO¹, GA-HUI KANG¹, KYOUNGOOK KIM³, JEUNG-SUL HAN^1,4,*

BIOCELL, Vol.45, No.1, pp. 199-215, 2021, DOI:10.32604/biocell.2021.012353

Abstract Solanum lycopersicum ‘Heinz 1706’ is a pioneer model cultivar for tomato research, whose whole genome sequence valuable for genomics studies is available. Nevertheless, a genetic transformation procedure for this cultivar has not yet been reported. Meanwhile, various genome editing technologies such as transfection of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) ribonucleoprotein complexes into cells are in the limelight. Utilizing the Cas9-expressing genotype possessing a reference genome can simplify the verification of an off-target effect, resolve the economic cost of Cas9 endonuclease preparation, and avoid the complex assembly process together with single-guide RNA (sgRNA) in the transfection approach. Thus,… More >

Monika Sood¹, Shanti S. Sharma¹, Joginder Singh¹, Ram Prasad^2,3,*, Dhriti Kapoor^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 71-86, 2020, DOI:10.32604/phyton.2020.07180

Abstract The heavy metals present in the environment accumulate in the plants and affect their productivity and yield. By entering the food chain, metals cause several serious health problems in human beings as well as in other organisms. Indole acetic acid (IAA) is known to act as a signaling molecule between symbiotic association of metal accumulating plants and plant growth promoting rhizobacteria (PGPR). Present study demonstrated a protective role of IAA against surplus Zinc (Zn)-induced toxicity to Hordeum vulgare seedlings. Elevated Zn concentrations suppressed the plant growth, caused a reduction in leaf relative water contents (RWC) and elevated free proline and… More >

Dandan Wang^1,#, Yulin Guo^1,#, Xiaofei Long¹, Yan Pan¹, Dingjie Yang¹, Rui Li¹, Ye Lu¹, Ying Chen^1,2, Jisen Shi¹, Jinhui Chen^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 27-34, 2020, DOI:10.32604/phyton.2020.08971

Abstract In order to study how exogenous hormones in C. lanceolata (gymnosperm) regulate somatic embryogenesis, we measured the endogenous phytohormones of two genotypes with different somatic embryogenesis efficiency and found that an increase in endogenous concentrations of IAA and ABA may be correlated to more efficient somatic embryogenesis. By applying exogenous spermidine, we found that exogenous hormones may affect somatic embryogenesis efficiency through affecting the endogenous phytohormone content. Based on these results, further studies can be conducted whereby the concentration of exogenous hormones or the levels of endogenous phytohormones by molecular methods are regulated to promote somatic embryogenesis. Our research may… More >