ZIYU LIU^1,2, AKIKO OKANO^3,4, EMIKO SANADA^1,3,4, YUSHI FUTAMURA^3,4, TOSHIHIKO NOGAWA^3,5, KOSUKE ISHIKAWA⁶, KENTARO SEMBA^7,8, JIANG LI⁹, XIAOMENG LI¹⁰, HIROYUKI OSADA^3,4,11,*, NOBUMOTO WATANABE^1,2,4,*

Oncology Research, Vol.31, No.5, pp. 655-666, 2023, DOI:10.32604/or.2023.030248

Abstract

Myc belongs to a family of proto-oncogenes that encode transcription factors. The overexpression of c-Myc causes many types of cancers. Recently, we established a system for screening c-Myc inhibitors and identified antimycin A by screening the RIKEN NPDepo chemical library. The specific mechanism of promoting tumor cell metastasis by high c-Myc expression remains to be explained. In this study, we screened approximately 5,600 microbial extracts using this system and identified a broth prepared from Streptomyces sp. RK19-A0402 strongly inhibits c-Myc transcriptional activity. After purification of the hit broth, we identified compounds closely related to the aglycone of cytovaricin and had… More > Graphic Abstract

Armin Saed-Moucheshi^1,*, Elham Rezaei Mirghaed²

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 1921-1941, 2023, DOI:10.32604/phyton.2023.027810

Abstract

One of the main reasons of the annual reduction in plant production all around the world is the occurrence of abiotic stresses as a result of an unpredicted changes in environmental conditions. Abiotic stresses basically trigger numerous pathways related to oxygen free radicals’ generation resulting in a higher rate of reactive oxygen species (ROS) production. Accordingly, higher rate of oxygen free radicals than its steady state causes to oxidize various types of molecules and compartments within the plants’ cells and tissues. Oxidative stress is the result of high amount free radicals of oxygen interfering with different functions leading to undergo… More >

ANA CECILIA LIAUDAT, FRANCISCA EBEL, BIANCA ANA OPIZZO, MARINA AURORA GONZALEZ, DAMIÁN BLOIS, PABLO BOSCH^#, NANCY RODRIGUEZ^#,*

BIOCELL, Vol.47, No.5, pp. 1135-1143, 2023, DOI:10.32604/biocell.2023.027628

Abstract Background: Capacitation is a set of physiological changes sperms undergo to acquire fertilizing capacity. In vivo, this process is directly associated with high calcium levels in sperm cytoplasm. Calcitriol, the vitamin D hypercalcemic metabolite, is related to human sperm motility, capacitation, and acrosome reaction. This work aimed to study the effect of calcitriol on bull sperm quality parameters and capacitation. Methods: One million freeze-thawed spermatozoa were obtained from different bulls and treated with 20 nM of calcitriol for 30 min. Untreated cells (negative control) and treated ones with calcitriol or heparin (100 µg/mL, positive capacitation control) were evaluated for motility,… More >

Shah Mohammad Naimul Islam¹, Niloy Paul¹, Md. Mezanur Rahman², Md. Ashraful Haque¹, Md. Motiar Rohman³, Mohammad Golam Mostofa^4,*

Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1513-1537, 2023, DOI:10.32604/phyton.2023.025175

Abstract Drought is a severe environmental constraint, causing a significant reduction in crop productivity across the world. Salicylic acid (SA) is an important plant growth regulator that helps plants cope with the adverse effects induced by various abiotic stresses. The current study investigated the potential effects of SA on drought tolerance efficacy in two barley (Hordeum vulgare) genotypes, namely BARI barley 5 and BARI barley 7. Ten-day-old barley seedlings were exposed to drought stress by maintaining 7.5% soil moisture content in the absence or presence of 0.5, 1.0 and 1.5 mM SA. Drought exposure led to severe damage to both genotypes,… More >

Savita Bhardwaj¹, Tunisha Verma¹, Ali Raza^2,*, Dhriti Kapoor^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 763-782, 2023, DOI:10.32604/phyton.2023.025672

Abstract

Arsenic (As) contaminated food chains have emerged as a serious public concern for humans and animals and are known to affect the cultivation of edible crops throughout the world. Therefore, the present study was designed to investigate the individual as well as the combined effects of exogenous silicon (Si) and sodium nitroprusside (SNP), a nitric oxide (NO) donor, on plant growth, metabolites, and antioxidant defense systems of radish (Raphanus sativus L.) plants under three different concentrations of As stress, i.e., 0.3, 0.5, and 0.7 mM in a pot experiment. The results showed that As stress reduced the growth parameters of… More >

Zhishu Jiang^#, Cong Gan^#, Yulian Liu, Xiaoli Lin, Limei Peng, Yongping Song, Xiaowei Luo, Jie Xu^*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 665-677, 2023, DOI:10.32604/phyton.2023.025266

Abstract Senescence is the last developmental process in plant, which has an important impact on crop yield and quality. In this study, a stable hereditary early-senescence line BC64 was isolated from the high-generation recombinant inbred lines of 93-11 and Wuyunjing7 (W7). Genetic analysis showed that the premature aging phenotype was controlled by a dominant gene derived from 93-11. By linkage analysis, the gene was primarily mapped in the region between marker B4 and B5 near the centromere of chromosome 4, described as ES(4). Through multiple backcrossing with W7, the near-isogenic line of ES(4) (NIL-ES(4)) was obtained. Compared with wild-type W7, NIL-ES(4)… More >

LINSHEN XIE¹, QIAOLAN WANG¹, JINGXUAN MA¹, YE ZENG^2,*

BIOCELL, Vol.47, No.2, pp. 261-267, 2023, DOI:10.32604/biocell.2023.024738

Abstract Fibrosis is the end-stage change of damaged tissues in various human diseases, which can lead to permanent scarring or organ malfunction. Hypoxia leads to oxidative stress, mitochondrial dysfunction, and inflammation in dysfunctional organs and tissues. Oxidative stress resulting from the overproduction of reactive oxygen species plays a central role in the fibrosis of injured organs. This review addresses the updated knowledge of the relationship between hypoxia and tissue fibrosis mediated by the reactive oxygen species pathway. Moreover, novel anti-fibrotic strategies are discussed, which may suppress reactive oxygen species and organ fibrosis. More >

TIANZHU LI^1,#, YU ZHANG^2,#, TONG ZHANG^2,#, YANNAN LI², HUI XUE², JINGLONG CAO², WENSHUANG HOU², YINGHUA LUO^3,*, CHENGHAO JIN^2,4,*,

BIOCELL, Vol.47, No.1, pp. 195-204, 2023, DOI:10.32604/biocell.2023.025593

Abstract Schisandrin B (Sch B) is a monomer with anti-cancer and anti-inflammatory effects, which are isolated from the plant Schisandra chinensis (Turcz) Baillon. We investigated the anti-gastric cancer (GC) effects of Sch B and its underlying molecular mechanisms. The Cell Counting Kit-8 assay was used to determine the effects of Sch B on the viability of GC and normal cell lines. Hoechst/propidium iodide staining and flow cytometry were used to assess the apoptosis induction of Sch B. Western blotting was used to evaluate the effects of Sch B on downstream apoptotic proteins. The DCFH-DA fluorescent probe was used to assess the… More >

WANTONG LIU^1,#, DANYANG CHEN^1,#, JINGYAO SU^1,#, RUILIN ZHENG^1,#, RAN KONG¹, BING ZHU¹, HAO DONG^2,*, YINGHUA LI^1,*

BIOCELL, Vol.47, No.1, pp. 187-194, 2023, DOI:10.32604/biocell.2022.023030

Abstract Liver cancer is the seventh most common malignant tumor in the world and is the second highest cause of death due to cancer. Quercetin, a flavonoid with low toxicity, widely exists in various fruits and vegetables. It has the potential to be a therapeutic agent against various cancers. This study aimed to demonstrate the anti-tumor effect of quercetin on HepG2 cells. Quercetin suppressed the HepG2 cell proliferation in a dose-dependent manner in cell viability assay. Induction of cell apoptosis was confirmed by apoptotic cells population (sub-G1 peak) detected by flow cytometer. A decrease in mitochondrial membrane potential and caspase-3 activation… More >

EKHLAQUE A. KHAN^1,2,*, HAMDINO M. I. AHMED³, MEENA MISRA¹, PALLAVI SHARMA^1,4, AMARENDRA N. MISRA^1,5, MIRZA HASANUZZAMAN^6,*

BIOCELL, Vol.46, No.12, pp. 2583-2593, 2022, DOI:10.32604/biocell.2022.021732

Abstract

Cadmium (Cd) causes oxidative stress, which leads to the oxidation of various biomolecules by the production of reactive oxygen species (ROS) to facilitate programmed cell death (PCD). The antioxidant defense system fails to detoxify ROS when it is produced in excess. Nitric oxide (NO), a gaseous free radical and a phytohormone, regulates various physiological processes of plants. Therefore, this work was undertaken to study the effects of the application of exogenous sodium nitroprusside (SNP, a NO donor) on growth parameters, oxidative stress, accumulation of secondary metabolites, and activities of antioxidant enzymes under Cd stress. Mild (50 µM) and severe (200… More >