TIECHENG SUN^1,#, YANDONG ZHANG^1,#, JIANHUA LI^3,#, HONG YU¹, LINGLI SONG¹, XINPING SUN¹, XI CHEN^2,*, LI TIAN^1,2,*

BIOCELL, Vol.45, No.3, pp. 705-710, 2021, DOI:10.32604/biocell.2021.014694

Abstract This study aims to investigate the protective effects of peroxiredoxin 6 on the total motility and progressive motility of human spermatozoa. Semen samples with normal parameters were collected from 23 males and supplemented with different concentrations of peroxiredoxin 6. All the semen samples were measured according to the WHO 5th manual, and the motile spermatozoa were extracted using IVF fertilization medium supplemented with different peroxiredoxin 6 concentrations. Total motility and progressive motility were observed at different timepoints of culture at room temperature. After peroxiredoxin 6 supplementation, all groups had a significant increase in total motility and progressive motility compared to… More >

YONG LIU^1,2,#, ZHIHUA JU^1,#, QIANG JIANG¹, WENHAO LIU¹, CHUNHONG YANG¹, YARAN ZHANG¹, XIUGE WANG¹, YAPING GAO¹, XIAOCHAO WEI¹, YAN SUN¹, JINPENG WANG¹, MINGHAI HOU¹, LING YANG², JINMING HUANG^1,*

BIOCELL, Vol.45, No.1, pp. 79-87, 2021, DOI:10.32604/biocell.2021.013583

Abstract F-box and WD-40 domain protein 11 (FBXW11) is an important component of the E3 ubiquitin-ligase enzyme that plays a key role in the ubiquitin-dependent regulation of spermatogenesis. In our previous research, the mRNA expression of FBXW11 in bull sperm with high motility is significantly higher than that with low motility. In the present study, the protein expression levels of FBXW11 in bull testicular tissues with low-performance sperm quality groups were significantly higher than those in normal performance groups. The immunohistochemistry result demonstrated that FBXW11 protein was located in the periphery of Leydig cells and seminiferous tubules. Three splice variants of… More >

WEI LIU^1,#, ZI WANG^1,2,#, JING LENG¹, HENG WEI¹, SHEN REN^1,2, XIAOJIE GONG³, CHEN CHEN⁴, YINGPING WANG^1,2, RUI ZHANG^1,2,*, WEI LI^1,2,*

BIOCELL, Vol.44, No.4, pp. 655-669, 2020, DOI:10.32604/biocell.2020.013202

Abstract Heat stress (HS) reaction can lead to serious physiological dysfunction associated with cardiovascular and various organ diseases. Ginsenoside Rg3 (G-Rg3) is a representative component of ginseng rare saponin and can protect against multiple organs, also used as functional food to adjust the balance of the human body, but the therapeutic effect and molecular mechanism of G-Rg3 on male diseases under HS are underexplored. The aim of the present study, G-Rg3 was prepared through the efficient conversion of ginsenoside Rd and investigate the contribution of G-Rg3 to testicular injury induced exposure to HS. All mice were divided into four groups as… More >

Sen Wang¹, Fei Zheng¹, Meijing Zhang¹, Jun Tu², Yanping Chen^1,3, Jianhua Yuan^1,3, Qingchang Meng^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 861-871, 2020, DOI:10.32604/phyton.2020.011762

Abstract Endosperm mutants are critical to the studies on both starch synthesis and metabolism and genetic improvement of starch quality in maize. In the present study, a novel maize endosperm mutant A0178 of natural variation was used as the experimental material and identified and then characterized. Through phenotypic identification, genetic analysis, main ingredients measurement and embryo rescue, development of genetic mapping population from A0178, the endosperm mutant gene was located. The results showed that the mutant exhibited extremely low germination ability as attributed to the inhibited embryo development, and amounts of sugars were accumulated in the mutant seeds and more sugars… More >

TIECHENG SUN^1,#, LUYANG CHENG^2,#, JING MA^3,#, SHANJIE ZHOU¹, YANDONG ZHANG¹, WENDI ZHOU⁴, SHOULONG DENG⁵, SHUSONG WANG^3,*, TIAN LI^1,6,*

BIOCELL, Vol.44, No.3, pp. 323-327, 2020, DOI:10.32604/biocell.2020.011890

Abstract Sperm cryopreservation is useful in assisted reproductive technology and male fertility preservation. However, freezing and thawing significantly reduces the total and progressive motility of human spermatozoa. In the present study, we explored the effects of peroxiredoxin 6 (PRDX6) on total and progressive motility of human spermatozoa after cryopreservation. Semen samples of 20 males with normal parameters were collected and frozen in media supplemented with different concentrations of PRDX6 (0 mM, 10⁻⁵ mM, 10⁻⁷ mM, and 10⁻⁹ mM, respectively). Postthaw total and progressive motility of sperms were measured. The results showed that in comparison with 0 mM, the concentrations of 10⁻⁵… More >

Thiresia-Teresa Tzatzani¹, Evangelia Basdeki², Evangelia-Vasiliki Ladikou³, Marios-Ioannis N. Sotiras³, Georgios Panagiotakis⁴, Sryridon Lionakis⁴, Ioannis E. Papadakis^3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 645-656, 2020, DOI:10.32604/phyton.2020.010532

Abstract The purpose of this study was to investigate the effects of various presowing treatments on the germinability (final germination percentage) and germination rate of loquat seeds in order to increase seedling production in nurseries (applied research) as well as provide answers for important physiological issues related to loquat seeds and their seed coat (basic research). Three experiments were carried out with various pre-sowing treatments. These treatments included full or partial removal of seed coat (perisperm), partial cutting of cotyledons as well as moist chilling at 5°C for 13 days and/or soaking the seeds in water or 250 ppm gibberellic acid… More >

YI ZHENG^#, PENGFEI ZHANG^#, JINSHENG ZHU^#, LINGKAI ZHANG, WENXIAN ZENG^*

BIOCELL, Vol.44, No.2, pp. 201-207, 2020, DOI:10.32604/biocell.2020.08770

Abstract Spermatogenesis is a highly efficient and intricate process in the testis by which mature spermatozoa are produced daily to maintain lifelong male fertility. Essential to this process are spermatogonia capable of both proliferation and differentiation. Nevertheless, the underlying mechanisms for spermatogonial proliferation and differentiation remain poorly understood. MicroRNAs (miRNAs) are a category of non-coding small RNAs with regulatory functions by binding to the 3’ untranslated region (UTR) of the target mRNA. Previous studies have demonstrated that miRNAs are capable of modulating cell proliferation, differentiation and apoptosis, but the roles of individual miRNAs in spermatogonial fate determination remain largely elusive. Here,… More >

Dandan Wang^1,#, Yulin Guo^1,#, Xiaofei Long¹, Yan Pan¹, Dingjie Yang¹, Rui Li¹, Ye Lu¹, Ying Chen^1,2, Jisen Shi¹, Jinhui Chen^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 27-34, 2020, DOI:10.32604/phyton.2020.08971

Abstract In order to study how exogenous hormones in C. lanceolata (gymnosperm) regulate somatic embryogenesis, we measured the endogenous phytohormones of two genotypes with different somatic embryogenesis efficiency and found that an increase in endogenous concentrations of IAA and ABA may be correlated to more efficient somatic embryogenesis. By applying exogenous spermidine, we found that exogenous hormones may affect somatic embryogenesis efficiency through affecting the endogenous phytohormone content. Based on these results, further studies can be conducted whereby the concentration of exogenous hormones or the levels of endogenous phytohormones by molecular methods are regulated to promote somatic embryogenesis. Our research may… More >

C. GAMARRA-LUQUES, B.C. WINIK, I.A. VEGA, E.A. ALBRECHT, N.M. CATALAN, A. CASTRO-VAZQUEZ

BIOCELL, Vol.30, Suppl.S, pp. 345-357, 2006

Abstract This article has no abstract. More >

ANURADHA SEHRAWAT AND VIJAY KUMAR^*

BIOCELL, Vol.36, No.2, pp. 63-71, 2012, DOI:10.32604/biocell.2012.36.063

Abstract The flower of Butea monosperma (Lam.) (Fabaceae) has been used in traditional Indian medicine in the treatment of many ailments including liver disorders. To understand the pharmacological basis of its beneficial effects, the extracts of dried flowers in water, methanol, butanol, ethyl acetate and acetone were evaluated for free radical scavenging and pro-apoptotic activities in cell cultures (human hepatoma Huh-7 cell line and immortalized AML-12 mouse hepatocytes). Butrin and butein -the active constituents of flower extracts- were used as reference molecules. The levels of cell injury markers like lactate dehydrogenase, glutathione and lipid peroxidation and primary antioxidant enzymes glutathione S-transferase… More >