Qiuying Quan^*1, Fengyun Zhong^†1, Xinwei Wang^‡, Kai Chen^§, Lingchuan Guo^*

Oncology Research, Vol.27, No.7, pp. 779-788, 2019, DOI:10.3727/096504018X15442985680348

Abstract The aim of this study was to investigate the underlying mechanisms that transforming growth factor- (TGF- )-mediated epithelial-to-mesenchymal transition (EMT) in tumor cells contributes to 5-FU resistance. A series of experiments involving cell viability and caspase activity analyses, siRNA transfection, RNA isolation, and quantitative-PCR (qPCR) assay, cell migration analysis, Western blotting analysis of total protein and membrane protein were performed in this study. Mouse xenograft model was used to determine the effect of the PAR2 inhibitor in vivo. In this study, we found that protease-activated receptor 2 (PAR2) induction in 5-FU therapy is correlated with TGF- -mediated EMT and apoptosis… More >

Yang Zhang^*†, Min Zhu^‡, Yuanbo Sun^§, Wenyuan Li^*†, Ying Wang^*†, Weiguang Yu^¶

Oncology Research, Vol.27, No.3, pp. 379-387, 2019, DOI:10.3727/096504018X15199531937158

Abstract Breast cancer is one of the major malignancies with a mounting mortality rate in the world. Long noncoding RNA (lncRNA) cancer susceptibility candidate 2 (CASC2) has been identified to regulate the initiation and progression of multiple tumorous diseases according to previous studies. However, its biological role has been rarely reported in breast cancer. In the present study, lncRNA CASC2 was found to be significantly downregulated in breast cancer tissues and cell lines using real-time quantitative PCR. Furthermore, gain-offunction assays demonstrated that overexpression of lncRNA CASC2 significantly repressed breast cancer cell proliferation and metastasis. Moreover, CASC2 induced cell cycle arrest and… More >

Jun Shan Ruan^*†, Huan Zhou^*, Lin Yang^‡, Ling Wang^*, Zong Sheng Jiang^§, Hong Sun^*, Shao Ming Wang^*†

Oncology Research, Vol.27, No.5, pp. 593-600, 2019, DOI:10.3727/096504017X15051723858706

Abstract Transforming growth factor- 1 (TGF- 1)-induced epithelial–mesenchymal transition (EMT) of non-small cell lung cancer (NSCLC) may contribute to tumor metastasis. TGF- 1-induced EMT in H1975 cells (a human NSCLC cell line) resulted in the adoption of mesenchymal responses that were predominantly mediated via the TGF- 1–integrin signaling pathway. Ursolic acid has been previously reported to inhibit tumor growth and metastasis in several cancers. However, whether ursolic acid can attenuate TGF- 1-induced EMT in H1975 cells and its underlying mechanisms remain unknown. In this study, ursolic acid significantly attenuated the TGF- 1-induced decrease in E-cadherin level and elevated the level of… More >

Yong Wang^*, Rui-Zhi Jia^*, Shu Diao^*, Jun He^†, Li Jia^†

Oncology Research, Vol.28, No.2, pp. 203-212, 2020, DOI:10.3727/096504019X15761480623959

Abstract Despite the considerable knowledge on the involvement of microRNA-101 (miR-101) in the evolution of oral squamous cell carcinoma (OSCC), the underlying mechanisms remain obscure. In this study, miR-101 expression was markedly downregulated in the OSCC cell lines and tissues. Cell counting kit-8 (CCK-8), ethynyl deoxyuridine (EdU), and colony formation assays showed that miR-101 inhibited the proliferation of OSCC cells. Flow cytometry and caspase 3 activity assays indicated that miR-101 induced OSCC cell apoptosis. Transwell assays demonstrated that this miRNA also repressed OSCC cell migration and invasion. Moreover, tube formation assay showed that miR-101 abated the proangiogenesis of OSCC cells. Dual-luciferase… More >

Fengyun Hao^*1, Qingli Zhu^†, Lingwei Lu^†, Shukai Sun^‡, Yichuan Huang^§, Jinna Zhang^¶, Zhaohui Liu^†#, Yuanqing Miao^**, Xuelong Jiao^††, Dong Chen^††1

Oncology Research, Vol.28, No.4, pp. 345-355, 2020, DOI:10.3727/096504020X15834065061807

Abstract Anaplastic thyroid carcinoma (ATC) is resistant to standard therapies and has no effective treatment. Eukaryotic translation initiation factor 5A2 (EIF5A2) has shown to be upregulated in many malignant tumors and proposed to be a critical gene involved in tumor metastasis. In this study, we aimed to investigate the expression status of EIF5A2 in human ATC tissues and to study the role and mechanisms of EIF5A2 in ATC tumorigenesis in vitro and in vivo. Expression of EIF5A2 protein was analyzed in paraffin-embedded human ATC tissues and adjacent nontumorous tissues (ANCT) (n = 24) by immunochemistry. Expressions of EIF5A2 mRNA and protein… More >

Quan Wen¹, Fulei Jing^1,*, Changxian Zhang¹, Shibai Tang¹, Junjie Yang^2,*

CMES-Computer Modeling in Engineering & Sciences, Vol.132, No.2, pp. 361-391, 2022, DOI:10.32604/cmes.2022.019528

Abstract Thermally grown oxide (TGO) is a critical factor for the service life of thermal barrier coatings (TBC). Numerical simulations of the growth process of TGO have become an effective means of comprehensively understanding the progressive damage of the TBC system. At present, technologies of numerical simulation to TGO growth include two categories: coupled chemical-mechanical methods and mechanical equivalent methods. The former is based on the diffusion analysis of oxidizing elements, which can describe the influence of bond coat (BC) consumption and phase transformation in the growth process of TGO on the mechanical behavior of each layer of TBC, and has… More >

Elias Hossain¹, Mohammed Alshehri², Sultan Almakdi^2,*, Hanan Halawani², Md. Mizanur Rahman³, Wahidur Rahman⁴, Sabila Al Jannat⁵, Nadim Kaysar⁶, Shishir Mia⁴

CMC-Computers, Materials & Continua, Vol.72, No.1, pp. 1713-1746, 2022, DOI:10.32604/cmc.2022.024822

Abstract Diabetes Mellitus is one of the most severe diseases, and many studies have been conducted to anticipate diabetes. This research aimed to develop an intelligent mobile application based on machine learning to determine the diabetic, pre-diabetic, or non-diabetic without the assistance of any physician or medical tests. This study's methodology was classified into two the Diabetes Prediction Approach and the Proposed System Architecture Design. The Diabetes Prediction Approach uses a novel approach, Light Gradient Boosting Machine (LightGBM), to ensure a faster diagnosis. The Proposed System Architecture Design has been combined into seven modules; the Answering Question Module is a natural… More >

Adekunle Babajide Rowaiye^1,2, Moses Okonkwo Njoku³, Angus Nnamdi Oli², Sousan Akrami^4,5, Titilayo Asala¹, Ikemefuna Chijioke Uzochukwu⁶, Alex Akpa¹, Morteza Saki^4,5,*, Charles Okechukwu Esimone²

Oncologie, Vol.23, No.4, pp. 547-557, 2021, DOI:10.32604/oncologie.2021.019738

Abstract Nonsteroidal anti-inflammatory drugs have a number of side effects. However, some medicinal herbs have anti-inflammatory properties with few side effects. This study aimed to determine the effect of the aqueous extract of Gongronema latifolium (AEGL) leaves on the level of tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in rabbits. Enzyme-linked immunosorbent assay (ELISA) was performed to determine the effect of the AEGL on the activities of pro-inflammatory cytokine (TNFα), and regulatory cytokine (TGF-β) in rabbits. Also, a colorimetric method was performed to evaluate the levels of AST and ALT. Overall, the weight… More >

XUE JIANG^1,2, HONG ZHANG^1,2,*

BIOCELL, Vol.46, No.3, pp. 785-794, 2022, DOI:10.32604/biocell.2022.016167

Abstract Posterior capsular opacification (PCO) is linked to the pathological process of lens epithelial cells, which includes proliferation, migration, and epithelial-mesenchymal transition (EMT). Our goal was to investigate whether long noncoding RNA (lncRNA) XIST contributes to EMT via targeting miR-124/Slug axis in TGF-β2-induced SRA01/04 cells. EMT was induced by stimulating SRA01/04 cells with 10 ng/mL TGF-β2 for 24 h, and PCO microenvironment was simulated. The expressions levels of lncRNA XIST, miR-124, and Slug were measured by real-time polymerase chain reaction (RT-PCR) and western blot. The role and mechanism of lncRNA XIST in promoting EMT of TGF-β2-treated SRA01/04 cells were investigated by… More >

Fang Wu¹, Qian Yu¹, Kangran Wang¹, Hong Gao^2,3,*

International Journal of Mental Health Promotion, Vol.23, No.4, pp. 545-564, 2021, DOI:10.32604/IJMHP.2021.017877

Abstract A harmonious relationship between teachers and postgraduates presents the comprehensive value of psychological education, which guarantees training quality and acts as an effective carrier of mental health education of the postgraduates. Based on Bandura’s theory of reciprocal determinism and Austin’s input-output theory, this paper constructs a model concerning the value of psychological education in the tutor-student relationship. The path of realization is also explored in a structural equation model through a questionnaire survey of 1112 graduate supervisors and administrators in medical colleges. The experimental result indicates that (1) The interaction between teachers and postgraduates promotes the value of psychological education… More >