Juanjuan Yao^*, Liang Zhong^†, Pengqiang Zhong^*, Dongdong Liu^†, Zhen Yuan^†, Junmei Liu^*, Shifei Yao^*, Yi Zhao^*, Min Chen^*, Lianwen Li^*, Lu Liu^†, Beizhong Liu^*†

Oncology Research, Vol.27, No.7, pp. 809-818, 2019, DOI:10.3727/096504018X15451301487729

Abstract RAS-responsive element-binding protein 1 (RREB1) is a transcription factor that is implicated in RAS signaling and multiple tumors. However, the role of RREB1 in acute myeloid leukemia has not been studied. We found that RREB1 is overexpressed in AML patients and myeloid leukemia cell lines (NB4 and HL-60), and RREB1 expression was significantly decreased during granulocytic differentiation of myeloid leukemia cells induced by all-trans retinoic acid (ATRA). Then we performed a RREB1 knockdown assay in NB4 and HL-60 cells; the results showed that knockdown of RREB1 upregulated expression of CD11b, CEBP , and microRNA-145 (miR-145), which hinted that knockdown of… More >

Hossam Kamli^*, Gaffar S. Zaman^*, Ahmad Shaikh^*, Abdullah A. Mobarki^†, Prasanna Rajagopalan^*‡

Oncology Research, Vol.28, No.9, pp. 899-911, 2020, DOI:10.3727/096504021X16281573507558

Abstract Inhibition of the dihydroorotate dehydrogenase (DHODH) has been successful at the preclinical level in controlling myeloid leukemia. However, poor clinical trials warrant the search for new potent DHODH inhibitors. Herein we present a novel DHODH inhibitor SBL-105 effective against myeloid leukemia. Chemical characteristics were identified by ¹ H NMR, ¹³C NMR, and mass spectroscopy. Virtual docking and molecular dynamic simulation analysis were performed using the automated protocol with AutoDock-VINA, GROMACS program. Human-recombinant (rh) DHODH was used for enzyme inhibition study. THP-1, TF-1, HL-60, and SKM-1 cell lines were used. MTT assay was used to assess cell viability. Flow cytometry was… More >

XIAOFANG WANG¹, XIAOLIN TU^1,2,*, YUFEI MA¹, JIE CHEN¹, YANG SONG³, GUANGLIANG LIU¹

BIOCELL, Vol.46, No.9, pp. 2089-2099, 2022, DOI:10.32604/biocell.2022.020069

Abstract The limited bioactivity of scaffold materials is an important factor that restricts the development of bone tissue engineering. Wnt3a activates the classic Wnt/β-catenin signaling pathway which effects bone growth and development by the accumulation of β-catenin in the nucleus. In this study, we fabricated 3D printed PCL scaffold with Wnt3a-induced murine bone marrow-derived stromal cell line ST2 decellularized matrix (Wnt3a-ST2-dCM-PCL) and ST2 decellularized matrix (ST2-dCM-PCL) by freeze-thaw cycle and DNase decellularization treatment which efficiently decellularized >90% DNA while preserved most protein. Compared to ST2-dCM-PCL, Wnt3a-ST2-dCM-PCL significantly enhanced newly-seeded ST2 proliferation, osteogenic differentiation and upregulated osteogenic marker genes alkaline phosphatase (Alp),… More >

Abdon Atangana^1,2, Ilknur Koca^3,*

CMES-Computer Modeling in Engineering & Sciences, Vol.131, No.2, pp. 787-814, 2022, DOI:10.32604/cmes.2022.019221

Abstract Very recently, a new concept was introduced to capture crossover behaviors that exhibit changes in patterns. The aim was to model real-world problems exhibiting crossover from one process to another, for example, randomness to a power law. The concept was called piecewise calculus, as differential and integral operators are defined piece wisely. These behaviors have been observed in the spread of several infectious diseases, for example, tuberculosis. Therefore, in this paper, we aim at modeling the spread of tuberculosis using the concept of piecewise modeling. Several cases are considered, conditions under which the unique system solution is obtained are presented… More >

Salinda Sandamal^1,2,3,#, Asanka Tennakoon^1,#, Parakkrama Wijerathna¹, Song Ge^2,3, DABN Amarasekera⁴, Buddhi Marambe⁵, Sara M. Elwany⁶, Sobhy Sorour⁶, Ayman El Sabagh⁶, Mohamed M. Hassan⁷, Disna Ratnasekera^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1269-1287, 2022, DOI:10.32604/phyton.2022.018983

Abstract Information on the genetic diversity of wild rice species in Sri Lanka is relatively meagre, though it plays a key role in crop improvement programs of cultivated rice (Oryza sativa L.). The present study was carried out to identify the morphological variation pattern of the wild populations of O. nivara in Sri Lanka. Seven populations (P1 to P7) collected from different agro-ecological regions were characterized in a common garden based on nine morphological traits. The findings revealed a high level of phenotypic variation between populations when compared to within a population. The most variable traits were the flag leaf panicle… More >

MIN TANG^1,#, XUELING HE^1,2,#, XINGHONG YAO¹, JIRUI WEN¹, MINGYUE BAO¹, LIANG LI^1,*

BIOCELL, Vol.46, No.6, pp. 1465-1472, 2022, DOI:10.32604/biocell.2022.018967

Abstract The present study was designed to investigate the role of estrogen receptor α (ERα) in biaxial tensile strain (BTS) regulated osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMSCs). rBMSCs were derived from rats and overexpressed ERα. The rBMSCs were subjected to BTS at 1 Hz with a strain of 2% for 4 h per day, 3 days, with or without ERα inhibitor ICI 182,780 (ICI). Then, bone mineralization was performed by Alizarin Red Staining. The markers of osteogenic differentiation and downstream Wnt3a/β-catenin signaling were detected by western blotting. Results showed that BTS enhanced the osteogenic differentiation of rBMSCs,… More >

TAILIN WU^1,#; XIANG ZHOU^2,#; CANHUA YE¹; WENCAN LU¹; HAITAO LIN¹; YANZHE WEI¹; ZEKAI KE¹; ZHENGJI HUANG¹; JIANZHOU LUO¹; HUIREN TAO¹; CHUNGUANG DUAN^1,*

BIOCELL, Vol.46, No.4, pp. 1123-1123, 2022, DOI:10.32604/biocell.2022.020679

Abstract This article has no abstract. More >

HONG FU¹, HAOYU ZHAO¹, YALI YANG¹, SIYU WANG¹, KE DUAN^2,*, TAILIN GUO^1,*

BIOCELL, Vol.46, No.4, pp. 1067-1078, 2022, DOI:10.32604/biocell.2022.017435

Abstract CDK5 belongs to the cyclin-dependent kinase family. CDK5 is multifunctional and plays an important role in neural differentiation. However, the role of CDK5 in osteoblastic differentiation remains unclear. The present study investigated functions and molecular mechanism of CDK5 in osteoblastic differentiation. It was found that, CDK5 inhibition promoted the expression of Runx2, ALP, OCN and OPN of MSCs and the mineralization of MC-3T3E1 cells and MSCs. CDK5 inhibition enhanced the development of F-actin, nuclear localization of β-catenin and YAP, as well as the expression of RMRP RNA. When F-actin was suppressed by Blebbistatin, the nuclear localization of YAP and β-catenin,… More >

JIRUI WEN^1,#, WENCHAO WU^2,#, MIN TANG¹, MINGYUE BAO¹, XUELING HE³, XINGHONG YAO¹, LIANG LI^1,*

BIOCELL, Vol.46, No.3, pp. 655-665, 2022, DOI:10.32604/biocell.2022.016915

Abstract To dissect which subset of bone marrow monocyte is the major precursor of osteoclast, 3-month-old rat bone marrow was obtained for single-cell RNA sequencing. A total of 6091 cells were acquired for detailed analysis, with a median number of 1206 genes detected per cell and 17,959 genes detected in total. A total of 19 cell clusters were recognized, with the main lineages identified as B cells, Granulocytes, Monocytes, T cells, Erythrocytes and Macrophages. Monocytes were further divided into classical monocytes and non-classical monocytes. Compared with non-classical monocytes, classical monocytes highly expressed osteoclast differentiation related genes Mitf, Spi1, Fos and Csf1r.… More >

IVY LEUNG¹, MARIA-LUISA VEISAGA², MARGARITA ESPINAL¹, WEI ZHANG¹, ROBERT BARNUM¹, MANUEL ALEJANDRO BARBIERI^1,2,3,4,*

BIOCELL, Vol.46, No.3, pp. 567-578, 2022, DOI:10.32604/biocell.2022.014009

Abstract The Annona genus is a member of Annonaceae, one of the largest families of plants across tropical and sub-tropical regions. This family has been used in several ethnomedicinal practices to treat a multitude of human diseases. However, the molecular mechanism underlying its effect on the lipid droplet formation and on the expression of adipogenic markers of this plant remain to be investigated. In this study, we examined whether the extracts from the aerial part of Annona montana affect in vitro differentiation of preadipocytes. For our investigations, both mouse embryo fibroblast 3T3-L1 and normal human primary subcutaneous preadipocytes were incubated with… More >