MINGHONG WEI^1,#, CHENG XIAO^1,2,#, JIAN WU^1,3, LIHONG QIN^1,3, HONGLIANG LIU^1,3, YANG CAO^1,*, YUMIN ZHAO^1,3,*

BIOCELL, Vol.47, No.9, pp. 2115-2123, 2023, DOI:10.32604/biocell.2023.030364 - 28 September 2023

Abstract Background: The regulatory genes related to lipid metabolism affect the intramuscular fat (IMF) content and improve meat quality traits. Mediator Complex Subunit 4 (MED4), a vitamin D receptor protein, affects vitamin D, livestock growth, carcass traits, and triglyceride deposition. However, the physiological function of the MED4 gene on bovine adipocyte differentiation remains unknown. Methods: This study explored the function of the MED4 gene in preadipocyte differentiation in Chinese Red Steppe cattle. The overexpression plasmid and the interference sequences of the MED4 gene were constructed to detect the effects of the MED4 gene on adipogenesis and biomakers using More >

WENQI CHEN^1,#, XIAOYANG CHU^2,#, YANG ZENG^3,#, YOUSHENG YAN⁴, YIPENG WANG⁴, DONGLAN SUN¹, DONGLIANG ZHANG⁵, JING ZHANG^1,*, KAI YANG^4,*

BIOCELL, Vol.47, No.7, pp. 1561-1569, 2023, DOI:10.32604/biocell.2023.028851 - 21 June 2023

Abstract Background: Receptor tyrosine kinase-like orphan receptor 2 (ROR2) has a vital role in osteogenesis. However, the mechanism underlying the regulation of ROR2 in osteogenic differentiation is still poorly comprehended. A previous study by our research group showed that a novel compound heterozygous ROR2 variation accounted for the autosomal recessive Robinow syndrome (ARRS). This study attempted to explore the impact of the ROR2: c.904C>T variant specifically on the osteogenic differentiation of BMSCs. Methods: Coimmunoprecipitation (CoIP)-western blotting was carried out to identify the interaction between ROR2 and Wnt5a. Double-immunofluorescence staining was used for determining the expressions and co-localization… More > Graphic Abstract

LU LIU^1,2, BIN XIE^1,2, WEI ZHU^1,2, QIUYAN HE^1,2, JIANHUA ZHOU^1,2, SHUANG LIU³, YONGGUANG TAO⁴, DESHENG XIAO^1,2,*

Oncology Research, Vol.31, No.3, pp. 275-286, 2023, DOI:10.32604/or.2023.028227 - 22 May 2023

Abstract Background: Lung cancer is one of the most lethal cancers worldwide, but studies have shown that the higher the expression of programmed cell death protein 1 ligand 1 (PD-L1) in non-small cell lung cancer (NSCLC), the more likely it will benefit from anti-PD-L1 immunotherapy. The purpose of our study was to collect and analyze abundant clinical samples in order to provide evidence for clinicians and patients who might consider anti-PD-L1 immunotherapy while jointly formulating treatment plans. Methods: On the one hand, we obtained cases from The Cancer Genome Atlas (TCGA) database, including 498 lung squamous… More >

YANG CAO^1,#, YINING LIU^1,#, YANG YU¹, XIAOFEI GUO¹, XIUXIU WANG¹, WENYA MA¹, HANJING LI², ZHONGYU REN², XINLU GAO², SIJIA LI², HAOYU JI², HONGYANG CHEN², HONG YAN², YANAN TIAN², XIN WANG², BENZHI CAI^1,2,*

BIOCELL, Vol.47, No.6, pp. 1407-1416, 2023, DOI:10.32604/biocell.2023.029080 - 19 May 2023

Abstract Background: Cardiomyocytes derived from human embryonic stem cells (hESCs) are regulated by complex and stringent gene networks during differentiation. Long non-coding RNAs (lncRNAs) exert critical epigenetic regulatory functions in multiple differentiation processes. However, the involvement of lncRNAs in the differentiation of hESCs into cardiomyocytes has not yet been fully elucidated. Here, we identified the key roles of ZFAS1 (lncRNA zinc finger antisense 1) in the differentiation of cardiomyocytes from hESCs. Methods: A model of cardiomyocyte differentiation from stem cells was established using the monolayer differentiation method, and the number of beating hESCs-derived cardiomyocytes was calculated. Gene expression… More >

Muhammad Aadil Siddiqui^1,*, M. H. Md Khir¹, Zaka Ullah², Muath Al Hasan², Abdul Saboor³, Saeed Ahmed Magsi¹

CMC-Computers, Materials & Continua, Vol.75, No.2, pp. 2859-2871, 2023, DOI:10.32604/cmc.2023.034164 - 31 March 2023

Abstract One of the most pressing concerns for the consumer market is the detection of adulteration in meat products due to their preciousness. The rapid and accurate identification mechanism for lard adulteration in meat products is highly necessary, for developing a mechanism trusted by consumers and that can be used to make a definitive diagnosis. Fourier Transform Infrared Spectroscopy (FTIR) is used in this work to identify lard adulteration in cow, lamb, and chicken samples. A simplified extraction method was implied to obtain the lipids from pure and adulterated meat. Adulterated samples were obtained by mixing… More >

JESSICA CRISTINA MARÍN-LLERA^*, CARLOS AMAURY JIMÉNEZ-CÁRDENAS, JESÚS CHIMAL-MONROY^*

BIOCELL, Vol.47, No.3, pp. 465-471, 2023, DOI:10.32604/biocell.2023.024625 - 03 January 2023

Abstract The molecular cascade underlying tendon formation starts when progenitor cells begin to express the Scleraxis (Scx) gene. Scx knockout mice develop some but not all tendons, suggesting that additional factors are necessary for tendon commitment, maintenance, and differentiation. Other transcription factors, such as Mohawk (Mkx) or early growth response (Egr), maintain Scx expression and extracellular matrix formation during fibrillogenesis. The inhibition of wingless and int-related protein signaling is necessary and sufficient to induce the expression of Scx. Once the commitment of tenogenic lineage occurs, transforming growth factor-beta (TGFβ) induces the Scx gene expression, becoming involved in the maintenance of tendon More >

YUNG-FU CHANG^1,2,3,*

BIOCELL, Vol.47, No.2, pp. 283-287, 2023, DOI:10.32604/biocell.2023.025743 - 18 November 2022

Abstract Areca nut is used worldwide as a hallucinogenic addicting drug along the tropical belt. Arecoline, a toxic compound, is the most important alkaloid in areca nuts. The adverse effects of oral uptake and chewing of areca nut are well known. For example, the possibility of cancer caused by chewing areca nuts is widely discussed. Chewing areca nut has other adverse effects on other organs, including abnormal cell differentiation, oral cancer, and several other diseases. The use of areca nut is also associated with low birthweight. Skeletal musculature is the largest organ in the body and More >

Lin Yuan¹, Jingcheng Zhang^2,*, Quan Deng², Yingying Dong³, Haolin Wang², Xiankun Du²

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 611-628, 2023, DOI:10.32604/phyton.2022.023662 - 12 October 2022

Abstract Hyperspectral imaging technique is known as a promising non-destructive way for detecting plants diseases and pests. In most previous studies, the utilization of the whole spectrum or a large number of bands as well as the complexity of model structure severely hampers the application of the technique in practice. If a detection system can be established with a few bands and a relatively simple logic, it would be of great significance for application. This study established a method for identifying and discriminating three commonly occurring diseases and pests of wheat, i.e., powdery mildew, yellow rust… More >

SUKUMARAN ANIL^1,2,*, RAMYA RAMADOSS³, NEBU G. THOMAS⁴, JASMIN M. GEORGE⁴, VISHNUPRIYA K. SWEETY⁴

BIOCELL, Vol.47, No.1, pp. 71-80, 2023, DOI:10.32604/biocell.2023.024334 - 26 September 2022

Abstract Exfoliated deciduous or an extracted healthy adult tooth can be used to harvest, process, and cryogenically preserve dental pulp stem cells. Future stem cell-based regenerative medicine methods could benefit significantly from these mesenchymal stem cells. Teeth serve as a substantial source of mesenchymal stem cells, otherwise disposed of as medical waste. Care should be taken to store this treasure trove of stem cells. Collective responsibility of patients, dentists, and physicians is necessary to ensure that this valuable resource is not wasted and that every possible dental pulp stem cell is available for use in the More >

NINGYU LI^1,2,#, XIAOFANG CHEN^2,#,§, SUXIA GENG², PEILONG LAI², LISI HUANG², MINMING LI², XIN HUANG², CHENGXIN DENG², YULIAN WANG², JIANYU WENG², XIN DU^1,2,*

BIOCELL, Vol.47, No.1, pp. 133-141, 2023, DOI:10.32604/biocell.2022.022021 - 26 September 2022

Abstract The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. In this study, exosomes from bone marrow plasma were successfully extracted and identified. Assessment of miR-103-3p expression in exosomes isolated from BM in 34 MDS patients and 10 controls revealed its 0.52-fold downregulation in patients with MDS compared with controls (NOR) and was downregulated 0.55-fold in MDS-MSCs compared with NOR-MSCs. Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation More >