Yunhong Zhang^1,2,*, Yonghui Yang^1,2, Jiawei Mao^1,2

Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 185-212, 2024, DOI:10.32604/phyton.2023.046811

Abstract Alginate oligosaccharides (AOS) enhance drought resistance in wheat (Triticum aestivum L.), but the definite mechanisms remain largely unknown. The physiological and transcriptome responses of wheat seedlings treated with AOS were analyzed under drought stress simulated with polyethylene glycol-6000. The results showed that AOS promoted the growth of wheat seedlings and reduced oxidative damage by improving peroxidase and superoxide dismutase activities under drought stress. A total of 10,064 and 15,208 differentially expressed unigenes (DEGs) obtained from the AOS treatment and control samples at 24 and 72 h after dehydration, respectively, were mainly enriched in the biosynthesis of secondary metabolites (phenylpropanoid biosynthesis,… More >

CHANGMEI LONG, TINGTING YANG, YUJIE HAN, LIZHEN HAN^*

BIOCELL, Vol.47, No.6, pp. 1417-1430, 2023, DOI:10.32604/biocell.2023.027485

Abstract Background: The plant-growth-promoting rhizobacterium Tsukamurella tyrosinosolvens is a rare strain of actinomycete, in order to recognize and expand the ecological functions of rare actinomycetes. Methods: In this experiment, we studied the effect of Tsukamurella tyrosinosolvens P9 on the drought resistance of peanut by inoculating peanut seedlings in pots and measuring the growth and physiological indicators of peanut under drought stress and re-watering conditions. Results: The results showed that during drought stress, the relative water content of the soil and leaves, chlorophyll content, and stomatal length, width, and aperture were significantly decreased while the levels of malondialdehyde (MDA), H₂O₂ and stomatal… More >

Siyan Liu^1,*, Jinfeng Liu¹, Yuzhe Zhang¹, Yushi Jiang¹, Shaowang Hu¹, Andi Shi¹, Qiyao Cong¹, Shuyan Guan^2,*, Jing Qu², Yao Dan¹

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1465-1482, 2022, DOI:10.32604/phyton.2022.018836

Abstract Exploring the molecular mechanism of soybean response to drought stress, providing a basis for genetic improvement and breeding of heat-resistant varieties, relying on the transcriptome sequencing data of unpollinated ovary at the seven-leaf stage of soybean Jinong 18(JN18) and Jinong 18 mutant (JB18) soybeans, using reverse transcription, one gene in the sHSP family was cloned using PCR (RT-PCR) and it was named sHSP26. In this experiment, the soybean sHSP26 gene was successfully cloned by RT-PCR, the protein encoded by the sHSP26 gene was analyzed by bioinformatics, and the sHSP26 gene overexpression vector and CRISPR/Cas9 gene-editing vector were constructed. The positive… More >

Yuzhe Zhang^1,#, Guanglong Li^1,#, Shaowang Hu¹, Jinfeng Liu¹, Yushi Jiang¹, Siyan Liu^2,*, Shuyan Guan^2,*, Jing Qu², Dan Yao¹, Andi Shi¹, Yixuan Liu¹

Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1183-1198, 2022, DOI:10.32604/phyton.2022.018853

Abstract Soybean (Glycine max (L.) Merr.) is an important cultivated crop, which requires much water during its growth, and drought seriously affects soybean yields. Studies have shown that the expression of small heat shock proteins can enhance drought resistance, cold resistance and salt resistance of plants. In this experiment, soybean GmHsps_p23-like gene was successfully cloned by RT-PCR, the protein encoded by the GmHsps_p23-like gene was subjected to bioinformatics analysis, and the pCAMBIA3301-GmHsps_p23-like overexpression vector and pCBSG015-GmHsps_p23-like gene editing vector were constructed. Agrobacterium-mediated method was used to transform soybeans to obtain positive plants. RT-PCR detection, rehydration experiment and drought resistance physiological and… More >

Castro-Nava S¹, J Ortiz-Cereceres^2,†, M del C Mendoza-Castillo², AJ Huerta³

Phyton-International Journal of Experimental Botany, Vol.81, pp. 149-156, 2012, DOI:10.32604/phyton.2012.81.149

Abstract The aim of this study was to determine the efficiency of drought classification criteria that we previously reported for our grain sorghum genotypes. Two tolerant genotypes of grain sorghum [Sorghum bicolor (L.) Moench] (UAT-124 and UAT-152) and one susceptible (UAT-30) were subjected to drought under greenhouse conditions at either panicle initiation, flag leaf, or flowering. Results showed that the effects of drought depended on when drought stress occurred during development. Biomass of the three sorghum genotypes was significantly reduced at the flag leaf stage (48%) by drought stress, but the greatest reduction occurred in the susceptible genotype UAT-30 (71%). Results… More >