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  • Open Access

    ARTICLE

    Alleviation of Cadmium Toxicity to Medicago Truncatula by AMF Involves the Changes of Cd Speciation in Rhizosphere Soil and Subcellular Distribution

    Yuying Jiang1,#, Renhua Huang1,#, Lei Jiang1, Ke Chen1,*, Wenkun Zhu2,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 403-415, 2021, DOI:10.32604/phyton.2021.014376 - 07 February 2021

    Abstract Arbuscular mycorrhizal fungi (AMF) can improve plant tolerance to several abiotic stresses, including heavy metals, drought or salinity exposure. However, the role of AMF in alleviation of soil cadmium (Cd)-induced toxicity to plants is still largely unknown. In this study, Cd speciation in soil and subcellular distribution of Cd were used to characterize the roles of application AM fungi in the alleviation of Cd toxicity in alfalfa plants. Our results showed that the addition of Glomus mosseae in Cd contaminated soil (10 mg/Kg) significantly increased soil pH, cation exchange capacity (CEC) and organic matter in rhizosphere… More >

  • Open Access

    ARTICLE

    Interaction of Acaena elongata L. with Arbuscular Mycorrhizal Fungi under Phosphorus Limitation Conditions in a Temperate Forest

    Yasmin Vázquez-Santos1, Yuriana Martínez-Orea1, Javier Álvarez-Sánchez1, Noé Manuel Montaño2, Sara Lucía Camargo-Ricalde2, Silvia Castillo-Argüero1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 605-619, 2021, DOI:10.32604/phyton.2021.014208 - 07 February 2021

    Abstract The aim of this study was to analyze the performance of Acaena elongata colonized by arbuscular mycorrhizal fungi (AMF) to different phosphorus (P) concentrations, as a measure of AMF dependency. A. elongata, is a species from soils where P availability is limited, such as temperate forests. Our research questions were: 1) How do different P concentrations affect the AMF association in Acaena elongata, and 2) How does the AMF association influence A. elongata’s growth under different P concentrations? A. elongata’s growth, P content in plant tissue, AMF colonization and dependency were measured under four P concentrations: control (0 g P kg−1More >

  • Open Access

    ARTICLE

    Natarajania thailandica sp. nov. (Stilbosporaceae, Diaporthales) from Thailand

    Monika C. Dayarathne1, Rashika S. Brahmanage2, Kevin D. Hyde2,3, Yong Wang1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 677-686, 2021, DOI:10.32604/phyton.2021.012897 - 07 February 2021

    Abstract A fungus similar to the monotypic genus Natarajania, isolated from dead wood and collected in Thailand, is reported. Analysis of partial ribosomal LSU and a protein coding gene (RPB2) demonstrated that the new isolate belonged to Stilbosporaceae, Diaporthales and genetically different from N. indica. It is unique in producing synnematous conidiophores, smooth-walled conidiogenous cells and a flared collarette but lacks an elongated collar-canal which is distinct in the type species. Therefore, sequence data and morphological traits are used to introduce the new species, Natarajania thailandica. More >

  • Open Access

    ARTICLE

    AM Fungi and Piriformospora indica Improve Plant Growth of Pinus elliottii Seedlings

    Taoze Sun, Wei Tan, Yujie Yang, Hongna Mu*

    Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 171-178, 2021, DOI:10.32604/phyton.2020.013668 - 20 November 2020

    Abstract Pinus elliottii is an exotic afforestation pine extensively distributed in southern parts of China. In order to understand whether endophytic fungi can affect seedling growth of P. elliottii, Piriformospora indica (Pi), Funnelifcrmis mosseae (Fm), and Diversispora tortuosa (Dt) were inoculated respectively, and the non-inoculated group was set as control. The growth indexes, the contents of soluble sugar and soluble protein, and plant endogenous hormone levels in the leaves of P. elliottii, were analyzed. The results showed that Fm, Dt and Pi colonized the P. elliottii roots to form mycorrhizal structure and chlamydospores arranged in beads respectively. Three fungal inoculants exhibited the stimulated… More >

  • Open Access

    ARTICLE

    Species Diversity of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Hevea brasiliensis in Hainan Island, China

    Xiubing Gao1,3, Jiejie Lv2, Can Guo1, Anlong Hu3, Xiaomao Wu3, Zengping Li4,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 179-192, 2021, DOI:10.32604/phyton.2021.012968 - 20 November 2020

    Abstract Hevea brasiliensis is one of the important economic trees with a great economic value for natural rubber production. Symbiosis between roots of H. brasiliensis and arbuscular mycorrhizal fungi (AMF) is widely recognized, and can provide a range of benefits for both of them. Hainan Island harbors is one of the largest plantations of H. brasiliensis in China, whereas the information regarding the diversity of AMF in the rhizosphere of H. brasiliensis on this island is scarce. The diversity of AMF species in the rhizosphere of rubber tree plantations in Hainan was investigated in this study. A total of 72… More >

  • Open Access

    ARTICLE

    Inhibitory Effect of N, N-Dimethylhexadecylamine on the Growth of White-Rot Fungus Trametes versicolor (L.) in Wood

    Wilber Montejo-Mayo1, Eduardo Días-Rivera1, Mauro Martínez-Pacheco2, Abril Munro-Rojas1, Enrique Ambriz-Parra1, Crisanto Velázquez-Becerra1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 193-206, 2021, DOI:10.32604/phyton.2020.011402 - 20 November 2020

    Abstract Wood is an organic material that is a source of carbon of organisms called Wood-decay fungi, and to preserve the wood, various toxic compounds to man and the environment have been used. To analyze the effect of N,N-Dimethylhexadecylamine (DMHDA) on wood attacked by the rotting fungus Trametes versicolor L. We used an in vitro system to expose the fungus T. versicolor to different concentrations of the DMHDA (50, 150 and 450 μM). We quantified the diameter of mycelial growth and laccase activity, also, under these experimental conditions we studied morphological details of the organisms using different scanning equipment including scanning… More >

  • Open Access

    ARTICLE

    Antifungal Activity of Schinifoline Against Candida Albicans in Caenorhabditis Elegans

    Chengjie Shu1, Mengli Zhang1, Weiming Zhang1, Shihong Ma1, Guoqing Chen2 and Baojun Shi1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 317-324, 2019, DOI:10.32604/phyton.2019.07766

    Abstract Zanthoxylum schinifolium has been used as spices and traditional medicine in China for hundreds of years. A variety of active substances have been isolated from Zanthoxylum schinifolium using biological and chemical techniques. Among these substances, the effect of schinifoline has gradually attracted much attention. Candida albicans is one of the most common pathogens isolated from the gastrointestinal tract, vagina, and mouth in healthy individuals. In a healthy population, there are various mechanisms in host, such as the microbial flora, the epithelial barriers, and the innate immune system, that can control the presence of Candida albicans.… More >

  • Open Access

    ARTICLE

    In vitro evaluation of native entomopathogenic fungi and neem (Azadiractha indica) extracts on Spodoptera frugiperda

    Hernandez-Trejo A1, B Estrada-Drouaillet1, JA López-Santillán1, C Rios-Velasco2, SE Varela-Fuentes1, R Rodríguez-Herrera3, E Osorio-Hernández1

    Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 47-54, 2019, DOI:10.32604/phyton.2019.04611

    Abstract The control of Spodoptera frugiperda is based on synthetic insecticides, so some alternatives are the use of entomopathogenic fungi (EF) and neem extract. The objective of the study was to evaluate in vitro effectiveness of native EF and neem extracts on S. frugiperda larvae. Six EF were identified by DNA sequencing of ITS regions from three EF (Fusarium solani, Metarrhizium robertsii, Nigrospora spherica and Penicillium citrinum). They were evaluated in concentrations of 1 × 10⁸ spores/ mL. In addition, a second bioassay was carried out evaluating only F. solani, M. robertsii and N. sphaerica and… More >

  • Open Access

    ARTICLE

    Evaluation of Antioxidants, Total Phenolics and Antimicrobial Activities of Ethyl Acetate Extracts From Fungi Grown on Rice Straw

    Bahaa T. Shawky1, Mohammed Nagah1, Mosad A. Ghareeb2, Gamal M. El-Sherbiny3, Saad A. M. Moghannem3, Mohamed S. Abdel-Aziz1,*

    Journal of Renewable Materials, Vol.7, No.7, pp. 667-682, 2019, DOI:10.32604/jrm.2019.04524

    Abstract Rice straw (RS) collected from Egyptian agricultural environment (Al-Masied village-Alsharqia Governorate-Egypt) was used as substrate for three fungal isolates namely: 4b, 1Asp, Pleurotus ostreatus NRRL 3501 and mixed culture of (4b, 1Asp). Isolates number 4b and 1Asp were identified using the molecular technique (18S rRNA). Isolate 4b and 1Asp, were identified as Trichoderma saturnisporum MN1-EGY and Aspergillus niger MN2-EGY, respectively. Trichoderma saturnisporum MN1-EGY extract exhibited the highest antimicrobial activity compared to the other fungal extracts. Total phenolic, antimicrobial and antioxidant activities were also determined from the ethyl acetate extract of rice straw inoculated by these… More >

  • Open Access

    ARTICLE

    In vitro antifungal/fungistatic activity of manganese phosphite against soybean soil-borne pathogens

    Carmona MA1, E Simonetti2,3, ME Ravotti1, MM Sc,iani4, AG Luque4, NA Formento5, FJ Sautua1

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 265-269, 2017, DOI:10.32604/phyton.2017.86.265

    Abstract Soybean root and stem rots caused by soil-borne pathogens are diseases commonly found in soybean fields, and one of the most important causes of crop losses. In the present study, the mycelial sensitivity of Fusarium virguliforme, F. tucumaniae, Sclerotinia sclerotiorum and Macrophomina phaseolina was evaluated on potato dextrose agar media (25 mL) supplemented with different concentrations of manganese phosphite (MnPhi) diluted in water (0, 25, 37.5, 50, 100, 200, 300, 400, 500, 800 and 1000 μg/mL). Mycelial growth sensitivity was analyzed using logarithmic linear regression analysis. The MnPhi concentration needed to inhibit 50% of the mycelial growth (IC50) More >

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