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  • Open Access

    ARTICLE

    In vitro antifungal/fungistatic activity of manganese phosphite against soybean soil-borne pathogens

    Carmona MA1, E Simonetti2,3, ME Ravotti1, MM Sc,iani4, AG Luque4, NA Formento5, FJ Sautua1

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 265-269, 2017, DOI:10.32604/phyton.2017.86.265

    Abstract Soybean root and stem rots caused by soil-borne pathogens are diseases commonly found in soybean fields, and one of the most important causes of crop losses. In the present study, the mycelial sensitivity of Fusarium virguliforme, F. tucumaniae, Sclerotinia sclerotiorum and Macrophomina phaseolina was evaluated on potato dextrose agar media (25 mL) supplemented with different concentrations of manganese phosphite (MnPhi) diluted in water (0, 25, 37.5, 50, 100, 200, 300, 400, 500, 800 and 1000 μg/mL). Mycelial growth sensitivity was analyzed using logarithmic linear regression analysis. The MnPhi concentration needed to inhibit 50% of the mycelial growth (IC50) ranged from… More >

  • Open Access

    ARTICLE

    Morphological, molecular and pathogenic diagnostic of fungi in lawn’s root and neck in Mexico

    García-Munguía CA1, M Acosta-Ramos2, O Vázquez-Martinez3, M Pérez-Sato5, A García-Munguía4, O García-Munguía6, AM García-Munguía3

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 258-264, 2017, DOI:10.32604/phyton.2017.86.258

    Abstract The illnesses that cause root and neck rot in golf courses constitute a limiting factor for grass development worldwide. This is mainly true when management guidelines are not conducted. Our research was conducted in the Golf Club “Mexico”, located in Mexico, D.F. Isolates were taken from plants that presented yellowing symptoms and root and neck rot. The identified pathogenic fungi, isolated from axenic media, were Giberella avenacea, Fusarium oxysporum, F. oxysporum f. sp. cubense and Pythium ultimum. The molecular characterization showed a comparison of the DNA-obtained sequences which was morphologically determined as F. avenaceum and was molecularly corroborated as G.… More >

  • Open Access

    ARTICLE

    Antifungal Activity of Schinifoline Against Candida Albicans in Caenorhabditis Elegans

    Chengjie Shu1, Mengli Zhang1, Weiming Zhang1, Shihong Ma1, Guoqing Chen2 and Baojun Shi1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 317-324, 2019, DOI:10.32604/phyton.2019.07766

    Abstract Zanthoxylum schinifolium has been used as spices and traditional medicine in China for hundreds of years. A variety of active substances have been isolated from Zanthoxylum schinifolium using biological and chemical techniques. Among these substances, the effect of schinifoline has gradually attracted much attention. Candida albicans is one of the most common pathogens isolated from the gastrointestinal tract, vagina, and mouth in healthy individuals. In a healthy population, there are various mechanisms in host, such as the microbial flora, the epithelial barriers, and the innate immune system, that can control the presence of Candida albicans. However, when host immunity is… More >

  • Open Access

    ARTICLE

    In vitro evaluation of native entomopathogenic fungi and neem (Azadiractha indica) extracts on Spodoptera frugiperda

    Hernandez-Trejo A1, B Estrada-Drouaillet1, JA López-Santillán1, C Rios-Velasco2, SE Varela-Fuentes1, R Rodríguez-Herrera3, E Osorio-Hernández1

    Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 47-54, 2019, DOI:10.32604/phyton.2019.04611

    Abstract The control of Spodoptera frugiperda is based on synthetic insecticides, so some alternatives are the use of entomopathogenic fungi (EF) and neem extract. The objective of the study was to evaluate in vitro effectiveness of native EF and neem extracts on S. frugiperda larvae. Six EF were identified by DNA sequencing of ITS regions from three EF (Fusarium solani, Metarrhizium robertsii, Nigrospora spherica and Penicillium citrinum). They were evaluated in concentrations of 1 × 10⁸ spores/ mL. In addition, a second bioassay was carried out evaluating only F. solani, M. robertsii and N. sphaerica and the addition of vegetable oil.… More >

  • Open Access

    ARTICLE

    Evaluation of Antioxidants, Total Phenolics and Antimicrobial Activities of Ethyl Acetate Extracts From Fungi Grown on Rice Straw

    Bahaa T. Shawky1, Mohammed Nagah1, Mosad A. Ghareeb2, Gamal M. El-Sherbiny3, Saad A. M. Moghannem3, Mohamed S. Abdel-Aziz1,*

    Journal of Renewable Materials, Vol.7, No.7, pp. 667-682, 2019, DOI:10.32604/jrm.2019.04524

    Abstract Rice straw (RS) collected from Egyptian agricultural environment (Al-Masied village-Alsharqia Governorate-Egypt) was used as substrate for three fungal isolates namely: 4b, 1Asp, Pleurotus ostreatus NRRL 3501 and mixed culture of (4b, 1Asp). Isolates number 4b and 1Asp were identified using the molecular technique (18S rRNA). Isolate 4b and 1Asp, were identified as Trichoderma saturnisporum MN1-EGY and Aspergillus niger MN2-EGY, respectively. Trichoderma saturnisporum MN1-EGY extract exhibited the highest antimicrobial activity compared to the other fungal extracts. Total phenolic, antimicrobial and antioxidant activities were also determined from the ethyl acetate extract of rice straw inoculated by these fungi. The antioxidant scavenging concentration(SC50)… More >

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