Home / Advanced Search

  • Title/Keywords

  • Author/Affliations

  • Journal

  • Article Type

  • Start Year

  • End Year

Update SearchingClear
  • Articles
  • Online
Search Results (14)
  • Open Access

    ARTICLE

    High Throughput Image Analysis between Seed Traits Opens New Breeding Avenues in Tartary Buckwheat Germplasm

    Bo Hwan Kim1,#, Sheikh Mansoor2,#, Gyung Deok Han3, Ji Eun Park4, Wook Kim1,*, Yong Suk Chung2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2339-2347, 2024, DOI:10.32604/phyton.2024.056439 - 30 September 2024

    Abstract Recognizing the variation of genetic resources is the first step in selection. One of the most important variations in grain crops is the uniformity of seed grain weight, which can be converted into seed size. However, it has been challenging since it needs high labor costs and time to measure it on a large scale. The current study used an image analysis technique to measure the grain seed area of about 100 seeds per accession with 64 germplasm of Tartary buckwheat (Fagopyrum tataricum) to study variation among and within them. To understand the nature of variation, More >

  • Open Access

    ARTICLE

    Landscape of Sequence Variations in Homologous Copies of FAD2 and FAD3 in Rapeseed (Brassica napus L.) Germplasm with High/Low Linolenic Acid Trait

    Haoxue Wu#, Xiaohan Zhang§,#, Xiaoyu Chen, Kang Li, Aixia Xu, Zhen Huang, Jungang Dong, Chengyu Yu*

    Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 627-640, 2024, DOI:10.32604/phyton.2024.050321 - 28 March 2024

    Abstract Genetic manipulation (either restraint or enhancement) of the biosynthesis pathway of α-linolenic acid (ALA) in seed oil is an important goal in Brassica napus breeding. B. napus is a tetraploid plant whose genome often harbors four and six homologous copies, respectively, of the two fatty acid desaturases FAD2 and FAD3, which control the last two steps of ALA biosynthesis during seed oil accumulation. In this study, we compared their promoters, coding sequences, and expression levels in three high-ALA inbred lines 2006L, R8Q10, and YH25005, a low-ALA line A28, a low-ALA/high-oleic-acid accession SW, and the wildtype ZS11. The expression… More >

  • Open Access

    REVIEW

    Biochemical Mechanism Unlocking Their Potential Role in Salt Tolerance Mechanism of Zizyphus Germplasm

    Riaz Ahmad1,*, Hafiza Muniba Din Muhammad2, Safina Naz2, Meryam Manzoor2, Muhammad Ahsan Altaf3

    Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1539-1553, 2023, DOI:10.32604/phyton.2023.026672 - 09 March 2023

    Abstract Salinity is one of the major constraints reducing plant growth and yield. Irrigation with poor quality and brackish water to orchards is a major cause of stunted growth and low yield. The salt tolerance mechanism is one of the complicated genomic characters that is very problematic to develop in fruit trees and becomes much more severe at any growth and developmental stage. Osmotic stress and hormonal imbalances are major constraints causing low biomass production. Fruit tree tolerance/sensitivity is chiefly based on the activation of a defense system comprised of super-oxidase dismutase (SOD), peroxidase (POD) and… More >

  • Open Access

    ARTICLE

    Transcriptome Analysis of a Wild Eggplant Germplasm M239 in Response to Verticillium dahliae Infection

    Liyan Wu1, Jie Cheng1,2, Yaju Gong1, Rui Bao1, Zhibin Li1, Min Gui1,*, Guanghui Du2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 591-609, 2023, DOI:10.32604/phyton.2022.023481 - 12 October 2022

    Abstract In this study, wild eggplant germplasm No. M239, which is highly susceptible to Verticillium wilt, was used as the experimental material. The physiological and biochemical indices (SOD, PAL, MDA and soluble protein) of M239 roots were measured at different times (0, 12, 24, 36, 48, 60 and 72 h) post inoculation with Verticillium dahliae, and the key time points for the M239 response to Verticillium wilt infection were screened. Then, RNA-Seq technology was used to screen the differentially expressed genes (DEGs) in M239 roots at 0, 12 and 48 h post-inoculation (hpi). The transcriptional results of M239… More >

  • Open Access

    ARTICLE

    Molecular characterization of sweet potato (Ipomoea batatas [L.] Lam) germplasms for desirable traits by using simple sequence repeats markers

    KHANDAKAR ABU MD MOSTAFIZAR RAHMAN1,2, ABDUL SHUKOR JURAIMI2,*, MD. REZWAN MOLLA3, MUHAMMAD ASYRAF MD HATTA4, ZULKEFLY BIN SULAIMAN5, SHAMIMA SULTANA6, AHMED GABER7, BENUKAR BISWAS8, AKBAR HOSSAIN9,*

    BIOCELL, Vol.47, No.1, pp. 227-237, 2023, DOI:10.32604/biocell.2023.025135 - 26 September 2022

    Abstract Every breeding program that aims to create new and improved cultivars with desired traits mostly relies on information related to genetic diversity. Therefore, molecular characterization of germplasms is important to obtain target cultivars with desirable traits. Sweet potato [Ipomoea batatas (L.) Lam] is widely considered the world’s most important crop, with great diversity in morphological and phenotypic traits. The genetic diversity of 20 sweet potato germplasms originating from Bangladesh, CIP, Philippines, Taiwan, and Malaysia were compared, which was accomplished by genetic diversity analysis by exploring 20 microsatellite DNA markers for germplasm characterization and utilization. This information… More >

  • Open Access

    ARTICLE

    Development of a New Cold-Tolerant Maize (Zea mays L.) Germplasm Using the ICE1 Gene from Arabidopsis thaliana

    Jing Qu1, Shuang Liu2, Peng Jiao2, Zhenzhong Jiang2, Jianbo Fei2, Shuyan Guan1,*, Yiyong Ma1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1709-1719, 2022, DOI:10.32604/phyton.2022.018854 - 14 April 2022

    Abstract To develop cold-tolerant maize germplasms and identify the activation of INDUCER OF CRT/DRE-BINDING FACTOR EXPRESSION (ICE1) expression in response to cold stress, RT-PCR was used to amplify the complete open reading frame sequence of the ICE1 gene and construct the plant expression vector pCAMBIA3301-ICE1-Bar. Immature maize embryos and calli were transformed with the recombinant vector using Agrobacterium tumefaciens-mediated transformations. From the regenerated plantlets, three T1 lines were screened and identified by PCR. A Southern blot analysis showed that a single copy of the ICE1 gene was integrated into the maize (Zea mays L.) genomes of the three T1 generations.… More >

  • Open Access

    ARTICLE

    Population Structure Analysis and Genome-Wide Association Study of Tea (Camellia sinensis (L.) K untze) Germplasm in Qiannan, China, Based on SLAFSeq Technology

    Fen Zhang1, Weili Tian1, Lu Cen1, Litang Lv2, Xiaofang Zeng1, Yulu Chen1, Yichen Zhao2,*, Yan Li1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 791-809, 2022, DOI:10.32604/phyton.2022.018104 - 09 December 2021

    Abstract Duyun Maojian tea is a famous tea in China. In this study, the specific-locus amplified fragment (SLAF) sequencing method was used to analyze the population structure and conduct a genome-wide association study (GWAS) of 2 leaf traits of 123 tea plants in Qiannan, China. A total of 462,019 SLAF tags and 11,362,041 single-nucleotide polymorphism (SNP) loci were obtained. The results of phylogenetic tree analysis, cluster analysis, and principal component analysis showed that 123 tea germplasms were clustered into three groups, and the heterozygosity rates for Groups I, II, and II were 0.206, 0.224, and 0.34,… More >

  • Open Access

    ARTICLE

    Genetic diversity in eggplant (Solanum melongena L.) germplasm from three secondary geographical origins of diversity using SSR markers

    DEBI RANI DATTA1, MOHD RAFII YUSOP1,2,*, AZIZAH MISRAN2, MASHITAH JUSOH2, YUSUFF OLADOSU1, FATAI AROLU1, AZADUL HAQUE1, NADZIRAH M. SULAIMAN1

    BIOCELL, Vol.45, No.5, pp. 1393-1401, 2021, DOI:10.32604/biocell.2021.015321 - 12 July 2021

    Abstract Indo-Burmese region was the primary center of eggplant diversity from where the crop extended to several secondary origins of diversity. In this study, the genetic diversity among fifty-six eggplant accessions collected from three countries was assessed using sixteen polymorphic SSR markers to determine suitable parents for heterotic hybridization. The estimation of genetic diversity among the population of three countries (Bangladesh, Malaysia, and Thailand) varied from 0.57 to 0.74, with Shannon’s index value of 0.65. The mean value of expected heterozygosity and Nei’s index was 0.49, with an average PIC value of 0.83. A dendrogram was… More >

  • Open Access

    REVIEW

    Applications of Molecular Markers in Fruit Crops for Breeding Programs—A Review

    Riaz Ahmad1, Muhammad Akbar Anjum1,*, Safina Naz1, Rashad Mukhtar Balal2

    Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 17-34, 2021, DOI:10.32604/phyton.2020.011680 - 20 November 2020

    Abstract Selection and use of molecular markers for evaluation of DNA polymorphism in plants are couple of the most important approaches in the field of molecular genetics. The assessment of genetic diversity using morphological markers is not sufficient due to little differentiating traits among the species, genera or their individuals. Morphological markers are not only highly influenced by environmental factors but skilled assessment is also prerequisite to find the variations in plant genetic resources. Therefore, molecular markers are considered as efficient tools for detailed DNA based characterization of fruit crops. Molecular markers provide new directions to More >

  • Open Access

    ARTICLE

    Analysis of the Relationship between Blast Resistance Genes and Disease Resistance of Rice Germplasm via Functional Molecular Markers

    Ning Xuan1, Hua Zhang1, Xu Liu1, Qiang Guo1, Jian Liu2, Fangyin Yao1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 45-55, 2020, DOI:10.32604/phyton.2020.08548 - 01 March 2020

    Abstract Rice blast disease is one of the most devastating diseases of rice (Oryza sativa L.) caused by the fungus Magnaporthe oryzae (M. oryzae), and neck blast is the most destructive phase of this illness. The underlying molecular mechanisms of rice blast resistance are not well known. Thus, we collected 150 rice varieties from different ecotypes in China and assessed the rice blast resistances under the natural conditions that favoured disease development in Jining, Shandong Province, China in 2017. Results showed that 92 (61.3%) and 58 (38.7%) rice varieties were resistant and susceptible to M. oryzae, respectively. Among the 150… More >

Displaying 1-10 on page 1 of 14. Per Page