Susan Azizmohammadi^*, Sima Azizmohammadi^*, Aghdas Safari^†, Maria Kaghazian^‡, Mina Sadrkhanlo^§, Vahid Behnod^¶, Mehri Seifoleslami^#

Oncology Research, Vol.25, No.4, pp. 495-501, 2017, DOI:10.3727/096504016X14749735594687

Abstract The investigation of specific genes will establish more useful biomarkers for accurate detection and management of gynecological cancers, especially patients with cervical cancer (CCP). The aim of this study was to evaluate the expression level of RIPK4 and EZH2 messenger RNA (RIPK4 and EZH2 mRNA) in CCP. Expression of RIPK4 and EZH2 in the tissues was determined by immunohistochemistry and qRT-PCR methods. Correlations of RIPK4 and EZH2 mRNA with clinical and pathological parameters were analyzed using the Fisher’s exact test. The mRNA level of RIPK4 was significantly upregulated in tumor tissues compared with matched adjacent… More >

Ravi Verma^a, Nirmal Verma^a, Jaishree Paul

European Cytokine Network, Vol.24, No.3, pp. 130-138, 2013, DOI:10.1684/ecn.2013.0343

Abstract Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the gastrointestinal tract involving aberrant activation of innate and adaptive immune responses. We aimed to study the expression profiles of the susceptibility gene Nod1 and selected pro- and anti- inflammatory cytokines during different stages of UC. Methods: 65 patients with mild, severe or remission stage of UC, and 22 normal colon mucosal biopsies from control individuals were included in the study for measuring the expression of nucleotide binding oligomerization 1(Nod1) and related pro- and anti-inflammatory cytokines using quantitative reverse transcription-PCR (qRT-PCR). mRNA expression levels were… More >

Orlando J. CASTEJÓN

BIOCELL, Vol.37, No.2, pp. 29-36, 2013, DOI:10.32604/biocell.2013.37.029

Abstract The present paper shows by means of confocal laser scanning microscopy the immunoreactivity of rat cerebellar Lugaro cells for calbindin, synapsin-I, PSD-95, GluR1, CaMKII alpha, and N-cadherin. Lugaro cells were easily characterized by their location beneath Purkinje cells. Calbindin revealed immunoreactivity in the cell body, and the axonal and dendritic processes. Synapsin-I labelled the presynaptic endings on Lugaro cells. Synapsin-I and PSD-95 immunoreactivity demonstrated the localization of presynaptic and postsynaptic endings surrounding cell soma, corresponding to afferent extrinsic and intrinsic cerebellar fi bers. GluR1 immunoreactivity of the soma and cell processes indicates that Lugaro cells More >

ORLANDO J. CASTEJÓN^*

BIOCELL, Vol.36, No.1, pp. 1-29, 2012, DOI:10.32604/biocell.2012.36.001

Abstract The Purkinje cell and their synaptic contacts have been described using (1) light microsocopy, (2) transmission and scanning electron microscopy, and freeze etching technique, (3) conventional and field emission scanning electron microscopy and cryofracture methods, (4) confocal laser scanning microscopy using intravital stain FM64, and (5) immunocytochemical techniques for Synapsin-I, PSD9-5, GluR1 subunit of AMPA receptors, N-cadherin, and CamKII alpha. The outer surface and inner content of plasma membrane, cell organelles, cytoskeleton, nucleus, dendritic and axonal processes have been exposed and analyzed in a three-dimensional view. The intramembrane morphology, in bi- and three-dimensional views, and More >

Jiro Hirota¹, Shinya Shimizu¹, Atsushi Watanabe², Fumiko Suzuta³, Kazue Yajima⁴, Kumiko Kimura⁵, Makoto Haritani⁵, Shigeki Inumaru¹, Yukio Yagi⁶

European Cytokine Network, Vol.22, No.1, pp. 73-80, 2011, DOI:10.1684/ecn.2011.0275

Abstract Three IgG class anti-bovine CXCL8 (bCXCL8) monoclonal antibody (mAb)-secreting hybridomas, SH8-8D7, SH8-12A5 and SH8-2A1, were developed. SH8-8D7 was IgG2a, and SH8-12A5 and SH8-2A1 were IgG1. All three mAbs detected recombinant bCXCL8 (rbCXCL8) by immunoprecipitation and Western blotting. SH8- 2A1 could neutralise the chemotactic activity of rbCXCL8 towards neutrophils. The quantitative bCXCL8 ELISA was constituted by the combination of SH8-12A5 and biotin-SH8-2A1. The detection range was 20-1000 pg/mL. A sandwich ELISA was used to measure native bCXCL8 derived from the supernatant of cultured bovine peripheral blood mononuclear cells stimulated with ConA, LPS or PHA. Furthermore, SH8-2A1 More >

Marco Locatelli¹, Leonardo Boiocchi^1,2, Stefano Ferrero², Filippo Martinelli Boneschi³, Mario Zavanone^1,4, Samantha Pesce⁵, Paola Allavena⁵, Sergio Maria Gaini^1,4, Lorenzo Bello^1,4, Alberto Mantovani^5,6

European Cytokine Network, Vol.21, No.1, pp. 27-33, 2010, DOI:10.1684/ecn.2009.0184

Abstract The chemokine receptor CX3CR1 and its cognate ligand CX3CL1 (also known as fractalkine), are involved in central nervous system pathophysiology, in particular, in the cross-talk between neurons and micro-glia. It was therefore important to investigate the expression of CX3CR1 in gliomas, the most frequently occur-ring, malignant brain tumors. In a consecutive series of 70 patients with primary, central nervous glial tumors, CX3CR1 was highly expressed in tumor cells as assessed by RT-PCR mRNA and protein levels, and by immu-nohistochemistry, while the corresponding normal cells were negative. Receptor immuno-positivity did not correlate with histology, grade, chromosomal More >

ORLANDO J. CASTEJÓN^1*, MICHAEL E. DAILEY²

BIOCELL, Vol.33, No.2, pp. 71-80, 2009, DOI:10.32604/biocell.2009.33.071

Abstract The localization of GluR1 subunits of ionotropic glutamate receptors in the glial cells and inhibitory neurons of cerebellar cortex and their association with the climbing and parallel fibers, and basket cell axons were studied. Samples of P14 and P21 rat cerebellar cortex were exposed to a specific antibody against GluR1 subunit(s) of AMPA receptors and were examined with confocal laser scanning microscopy. GluR1 strong immunoreactivity was confined to Purkinje cell and the molecular layer. Weak GluR1 immunoreactivity was observed surrounding some Golgi cells in the granule cell layer. Intense GluR1 immunoreactivity was localized around Purkinje,… More >

Yoh Matsuoka^1,2, Makoto Hirokawa¹, Kimio Chiba¹, Takafumi Hashiba¹, Takeshi Tomoda¹, Shimpei Sugiura¹

Canadian Journal of Urology, Vol.15, No.3, pp. 4106-4108, 2008

Abstract We report a rare case of sarcomatoid carcinoma of the urinary bladder which showed both of biphasic and monophasic phenotypes through its clinical course. A 64 year-old man presented with gross hematuria and interrupted voiding. Radiological examination demonstrated a pedunculated tumor in the bladder with no distant metastases. Pathological diagnosis of transurethral resection revealed biphasic sarcomatoid carcinoma, pT1, composed of squamous cell carcinoma component and spindle cell component. The disease had repeated local recurrences and partial and total cystectomies were performed consequently. The recurrent tumors were monophasic sarcomatoid carcinoma purely composed of spindle cell component. More >

Yoh Matsuoka^1,3, Gaku Arai¹, Hisashi Ishimaru¹, Kentaro Takagi¹, Yuji Ito²

Canadian Journal of Urology, Vol.14, No.6, pp. 3764-3766, 2007

Abstract We report a rare case of primary signet-ring cell carcinoma of the prostate in an advanced stage. A 62-year-old man with serum level of prostate-specific antigen at 364.70 ng/ml was diagnosed as having cT4N1M1c prostatic signet-ring cell carcinoma of Gleason score 5 + 4 = 9. Immunohistochemical examination demonstrated cytoplasmic immunoreactivity to prostate-specific antigen in signet-ring cancer cells. The intracytoplasmic vacuoles in the signet-ring cells showed mucin production with a positive staining with periodic acid-Schiff. Although the patient received hormonal therapy, the disease progressed and lead to death 15 months after the diagnosis. The clinical More >

JOÃO PAULO MARDEGAN ISSA^*, RODRIGO TIOSSI^*, MAMIE MIZUSAKI IYOMASA^**

BIOCELL, Vol.31, No.3, pp. 375-382, 2007, DOI:10.32604/biocell.2007.31.375

Abstract The aim of this study was to evaluate alterations in the masseter muscle of 30 male guinea-pigs submitted to occlusal alteration. The animals were divided into 2 equal groups, the control group (C) only submitted to surgical stress, and the occlusal altered group (T) submitted to teeth extraction. Each group was subdivided into 3 groups, with 5 animals, for the following studies: macroscopy and vessels distribution, light microscopy and histochemical analysis, with animals’ perfusion 2 months after surgery. Data were submitted to statistical analysis. Macroscopically, an anteroposterior orientation of the muscular fibers was found and More >