LI TIAN^1,#, YONGYI HUANG^1,#, BAOZHEN ZHANG^2,#, YI SONG^1,#, LIN YANG³, QIANQIAN CHEN¹, ZHENG WANG³, YILING WANG¹, QIHAN HE¹, WENHAN YANG¹, SHUYONG YU⁴, TIANYU LU⁵, ZICHEN LIU¹, KAIPING GAO^1,*, XIUJUN FAN^2,*, JIAN SONG^4,*, RIHONG ZHAI^1,*

Oncology Research, Vol.31, No.4, pp. 463-479, 2023, DOI:10.32604/or.2023.028791

Abstract Accumulating evidence has indicated that long non-coding RNAs (lncRNAs) play critical roles in the development and progression of cancers, including esophageal squamous cell carcinoma (ESCC). However, the mechanisms of lncRNAs in ESCC are still incompletely understood and therapeutic attempts for in vivo targeting cancer-associated lncRNA remain a challenge. By RNA-sequencing analysis, we identified that LLNLR-299G3.1 was a novel ESCC-associated lncRNA. LLNLR-299G3.1 was up-regulated in ESCC tissues and cells and promoted ESCC cell proliferation and invasion. Silencing of LLNLR-299G3.1 with ASO (antisense oligonucleotide) resulted in opposite effects. Mechanistically, LLNLR-299G3.1 bound to cancer-associated RNA binding proteins and regulated the expression of cancer-related… More >

CHUYAO WANG^1,2,#, CHUAN LU^2,#, LUXIANG ZOU^2,*, DONGMEI HE^2,*

BIOCELL, Vol.47, No.6, pp. 1293-1306, 2023, DOI:10.32604/biocell.2023.028199

Abstract Background: Temporomandibular joint osteoarthritis (TMJOA) is an end-stage disease that seriously affects the patients’ quality of life. Molecular insights in advancing our understanding of TMJOA are the need of the hour. Methods: We performed RNA high-throughput sequencing and bioinformatics analysis of differentially expressed (DE) long non-coding RNA (lncRNAs), microRNAs (miRNAs), and messenger RNA (mRNAs) in human synovial TMJOA tissues. Firstly, synovium samples of TMJOA patients and non-TMJOA controls were collected for highthroughput sequencing of lncRNAs, miRNAs, and mRNAs. We then performed biological function analysis of the top 100 mRNAs with more than 2-fold differential expression, and their upstream regulated miRNAs… More >

YANG CAO^1,#, YINING LIU^1,#, YANG YU¹, XIAOFEI GUO¹, XIUXIU WANG¹, WENYA MA¹, HANJING LI², ZHONGYU REN², XINLU GAO², SIJIA LI², HAOYU JI², HONGYANG CHEN², HONG YAN², YANAN TIAN², XIN WANG², BENZHI CAI^1,2,*

BIOCELL, Vol.47, No.6, pp. 1407-1416, 2023, DOI:10.32604/biocell.2023.029080

Abstract Background: Cardiomyocytes derived from human embryonic stem cells (hESCs) are regulated by complex and stringent gene networks during differentiation. Long non-coding RNAs (lncRNAs) exert critical epigenetic regulatory functions in multiple differentiation processes. However, the involvement of lncRNAs in the differentiation of hESCs into cardiomyocytes has not yet been fully elucidated. Here, we identified the key roles of ZFAS1 (lncRNA zinc finger antisense 1) in the differentiation of cardiomyocytes from hESCs. Methods: A model of cardiomyocyte differentiation from stem cells was established using the monolayer differentiation method, and the number of beating hESCs-derived cardiomyocytes was calculated. Gene expression was analyzed by… More >

YANPING JIN¹, JIANPING QIU¹, XIUFANG LU¹, YAN MA¹, GUOWEI LI^2,*

Oncology Research, Vol.31, No.2, pp. 169-179, 2023, DOI:10.32604/or.2023.027815

Abstract Previous study revealed that ferritin heavy chain-1 (FTH1) could regulate ferritinophagy and affect intracellular Fe²⁺ content in various tumors, while its N6-methyladenosine (m6A) RNA methylation was closely related the prognosis of ovarian cancer patients. However, little is known about the role of FTH1 m6A methylation in ovarian cancer (OC) and its possible action mechanisms. In this study we constructed FTH1 m6A methylation regulatory pathway (LncRNA CACNA1G-AS1/IGF2BP1) according to related bioinformatics analysis and research, through clinical sample detections we found that these pathway regulatory factors were significantly up-regulated in ovarian cancer tissues, and their expression levels were closely related to the… More >

Yuan-Nong Ye^1,2,3,*, Ding-Fa Liang², Abraham Alemayehu Labena⁴, Zhu Zeng^2,*

Intelligent Automation & Soft Computing, Vol.36, No.3, pp. 2731-2741, 2023, DOI:10.32604/iasc.2023.026761

Abstract Essential ncRNA is a type of ncRNA which is indispensable for the survival of organisms. Although essential ncRNAs cannot encode proteins, they are as important as essential coding genes in biology. They have got wide variety of applications such as antimicrobial target discovery, minimal genome construction and evolution analysis. At present, the number of species required for the determination of essential ncRNAs in the whole genome scale is still very few due to the traditional methods are time-consuming, laborious and costly. In addition, traditional experimental methods are limited by the organisms as less than 1% of bacteria can be cultured… More >

LAKSHANA SADU^#, R.HARI KRISHNAN^#, R.L. AKSHAYA, I. SARANYA, UDIPT RANJAN DAS, SNEHA SATISHKUMAR, N. SELVAMURUGAN^*

BIOCELL, Vol.47, No.4, pp. 751-760, 2023, DOI:10.32604/biocell.2023.026911

Abstract MicroRNAs (miRNAs) are small non-coding RNAs (ncRNAs) that regulate the expression of their target mRNAs post-transcriptionally. Since their discovery, thousands of highly conserved miRNAs have been identified and investigated for their role in human health and diseases. MiR-214 has been increasingly reported to have an association with the regulation of bone metabolism. Reports suggested that miR-214 controls the critical aspects of osteoblasts (bone-forming cells), including their differentiation, proliferation, viability, and migration. Studies have also reported the functional significance of miR-214 in bone diseases and suggested its candidature as a diagnostic and therapeutic target. Further, targeting miR-214 by other ncRNAs, such… More >

WEI SHI^1,2,3,#, JIANXIA LIN^1,2,3,#, RONG JIN^1,2,3, XIANJING XIE^1,2,3, YAN LIANG^1,2,3,*

BIOCELL, Vol.47, No.4, pp. 869-877, 2023, DOI:10.32604/biocell.2023.026037

Abstract Background: LncRNA DLX6-AS1 has been uncovered to exert effects on various cancers. Nevertheless, the impacts of DLX6-AS1 on endometrial cancer (EC) development remained obscure. The study explored the influence of DLX6-AS1 on EC progression via the microRNA (miR)-374a-3p/zinc-finger protein (ZFX) axis.Methods: EC cell lines were collected and DLX6-AS1, miR-374a-3p, and ZFX levels in EC cell lines were detected. The EC cells were transfected with DLX6-AS1, miR-374a-3p, and ZFX constructs to examine the biological functions of EC cells. The xenograft model was established for detecting tumor growth. Rescue experiments were conducted to verify the interaction of DLX6-AS1, miR-374a-3p, and ZFX in… More > Graphic Abstract

QING GAO^1,#, YUJING SHI^2,#, YUANYUAN SUN^1,#, SHU ZHOU¹, ZEYUAN LIU³, XINCHEN SUN^1,*, XIAOKE DI^1,*

Oncology Research, Vol.31, No.1, pp. 35-61, 2023, DOI:10.32604/or.2022.028193

Abstract Aging is highly associated with tumor formation and progression. However, little research has explored the association of aging-related lncRNAs (ARLs) with the prognosis and tumor immune microenvironment (TIME) of head and neck squamous cell carcinoma (HNSCC). RNA sequences and clinicopathological data of HNSCC patients and normal subjects were downloaded from The Cancer Genome Atlas. In the training group, we used Pearson correlation, univariate Cox regression, least absolute shrinkage/selection operator regression analyses, and multivariate Cox regression to build a prognostic model. In the test group, we evaluated the model. Multivariate Cox regression was done to screen out independent prognostic factors, with… More >

SHAHRAM NEKOEIAN¹, TAHEREH ROSTAMI², AMIR NOROUZY³, SAFIN HUSSEIN^1,4, GHOLAMREZA TAVOOSIDANA¹, BAHRAM CHAHARDOULI², SHAHRBANO ROSTAMI^2,*, YAZDAN ASGARI⁵, ZAHRA AZIZI^1,*

Oncology Research, Vol.30, No.6, pp. 259-268, 2022, DOI:10.32604/or.2022.027904

Abstract Acute lymphoblastic leukemia (ALL) is a malignancy of bone marrow lymphoid precursors. Despite effective treatments, the causes of its progression or recurrence are still unknown. Finding prognostic biomarkers is needed for early diagnosis and more effective treatment. This study was performed to identify long non-coding RNAs (lncRNAs) involved in ALL progression by constructing a competitive endogenous RNA (ceRNA) network. These lncRNAs may serve as potential new biomarkers in the development of ALL. The GSE67684 dataset identified changes in lncRNAs and mRNAs involved in ALL progression. Data from this study were re-analyzed, and probes related to lncRNAs were retrieved. Targetscan, miRTarBase,… More >

RUI ZHOU^#, JIANYANG XU^#, LINGWEI WANG^*, JIANXIN LI^*

Oncology Research, Vol.29, No.6, pp. 411-423, 2021, DOI:10.32604/or.2022.026236

Abstract Long noncoding RNAs (lncRNAs) act as key regulators controlling complex cellular behaviors in nonsmall cell lung cancer (NSCLC). We investigated the expression of lncRNA PRRT3 antisense RNA 1 (PRRT3-AS1) in paired samples of NSCLC and adjacent normal tissues from a patient cohort in our hospital using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and found that it was significantly higher in NSCLC tissue than in normal tissue, consistent with The Cancer Genome Atlas database. Furthermore, functional investigation revealed that lncRNA PRRT3-AS1 depletion inhibited NSCLC-cell proliferation, colony formation, invasion, and migration, whereas its overexpression exerted the opposite effects. Moreover, PRRT3-AS1… More >