Mingda Yang¹, Shouchen Ma², Fujian Mei¹, Li Wei¹, Tongchao Wang^1,*, Xiaokang Guan^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 417-435, 2021, DOI:10.32604/phyton.2021.013175

Abstract Knowledge of the interactive effects of water and nitrogen (N) on physio-chemical traits of maize (Zea mays L.) helps to optimize water and N management and improve productivity. A split-plot experiment was conducted with three soil water conditions (severe drought, moderate drought, and fully water supply referring to 45%–55%, 65%–75%, and 85%–95% field capacity, respectively) and four N application rates (N₀, N₁₅₀, N₂₄₀, and N₃₃₀ referring to 0, 150, 240, 330 kg N ha^–1 respectively) under drip fertigation in 2014 and 2015 in the Huang-Huai-Hai Plain of China. The results indicated that drought stress inhibited physiological activity of plants (leaf… More >

Hans Christian Correa-Aguado^1,3, Gloria Viviana Cerrillo-Rojas¹, Alejandro Rocha-Uribe², Ruth Elena Soria-Guerra², José Francisco Morales-Domínguez^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 515-531, 2021, DOI:10.32604/phyton.2021.013619

Abstract The need for renewable energy sources makes microalgae an essential feedstock for biofuels production. The molecular aspects and the response to nitrogen (N)-limited conditions with a phytohormone stimulus in microalgae have been slightly explored. In this work, Scenedesmus obliquus was used as a study model to analyze the effect of benzyl amino purine (BAP) and gibberellic acid (GA) coupled to nitrogen limitation on cell growth, biomass and fatty acids. The selected 10^-5 M BAP increased the biomass by 1.44-fold, and 10^-6 M GA by 1.35-fold. The total lipids also increased by 2.8 and 1.11-fold, respectively. The 10^-5 M BAP and… More >

Tianyuan Li^1,#, Saif Ullah^1,#, He Liang¹, Izhar Ali¹, Quan Zhao¹, Anas Iqbal¹, Shanqing Wei¹, Tariq Shah², Yuqiong Luo¹, Ligeng Jiang^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 223-243, 2021, DOI:10.32604/phyton.2020.012065

Abstract Readily available chemical fertilizers have resulted in a decline in the use of organic manure (e.g., green manures), a traditionally sustainable source of nutrients. Based on this, we applied urea at the rate of 270 kg ha⁻¹ with and without green manure in order to assess nitrogen (N) productivity in a double rice cropping system in 2017. In particular, treatment combinations were as follows: winter fallow rice-rice (WF-R-R), milk vetch rice-rice (MV-R-R), oil-seed rape rice-rice (R-R-R) and potato crop rice-rice (P-R-R). Results revealed that green manure significantly (p ≤ 0.05) improved the soil chemical properties and net soil organic carbon… More >

Lei Chu^1,2, Yu Zhang^1,2, Long Qian^1,2, Dandan Zhu^1,2, Haijun Sun^1,2,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 1035-1042, 2020, DOI:10.32604/phyton.2020.014506

Abstract Biochar may affect the root morphology and nitrogen (N) use efficiency (NUE) of rice at seedling stage, which has not been clearly verified until now. To clarify it, we conducted a pot experiment regarding to two soil types (Hydragric Anthrosol and Haplic Acrisol), two biochar application rates (0.5 wt% and 1.5 wt %) and two rice varieties (common rice var. Xiushui134 and hybrid super rice var. Zhongkejiayou12-6) meanwhile. Seedling NUE of common rice Xiuhui134 was significantly increased (p < 0.05) by 78.2% in Hydragric Anthrosol and by 91.4% in Haplic Acrisol following biochar addition with 1.5 wt%. However, biochar addition… More >

Yanwei Feng¹, Wen Lei², Rui Gu³, Ping Zhao⁴, Shijun Ni^1,*, Ningfei Lei^3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 667-679, 2020, DOI:10.32604/phyton.2020.010160

Abstract Water, minerals, nutrients, etc., can be shared by physiological integration among inter-connected ramets of clonal plants. Nitrogen plays an important role in alleviating cadmium (Cd) stress for clonal plants. But how different forms of nitrogen affect growth performance of clonal plants subjected to heterogeneous Cd stress still remains poorly understood. A pot experiment was conducted to investigate the differential effects of ammonium and nitrate on growth performance of Glechoma longituba under heterogeneous Cd stress. In the experiment, parent ramets of Glechoma longituba clonal fragments were respectively supplied with modified Hoagland solution containing 7.5 mM ammonium, 7.5 mM nitrate or the… More >

Xiaohong Yin, Zui Tao, Jiana Chen, Fangbo Cao, Min Huang^*, Yingbin Zou

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 415-422, 2020, DOI:10.32604/phyton.2020.08962

Abstract It has been shown that adding biochar to soil can improve nitrogen (N) uptake and utilization in rice (Oryza sativa L.). However, there is a lack of research on the physiological alterations of rice as a result of the changes in nitrogen uptake due to the addition of biochar. This study conducted field experiments in 2015 and 2016 with the goal of testing the hypothesis that the application of biochar would enhance radiation use efficiency (RUE) of rice by improving the plant’s ability to take in and utilize nitrogen. Our results demonstrated that the application of biochar (20 t ha⁻¹… More >

Mohammed Fadhil HADDAD^1,2,3, Tugba DAYIOGLU^1,2, Barbaros NALBANTOĞLU², Turgay CAKMAK^1,*

BIOCELL, Vol.43, No.4, pp. 327-333, 2019, DOI:10.32604/biocell.2019.08522

Abstract Cyanobacteria are considered as a sustainable feedstock for the production of biochemically active compounds such as phycobiliproteins (PBPs). In this study, the impact of nitrogen (N) and phosphorus (P) availability on PBP production of “N-free acclimated” Anabaena variabilis IMU8 was analyzed. Upon isolation and identification, the cyanobacterium has been maintained in N-free BG-11 medium for more than 20 months. For experimentation, the strain was incubated in N-replete, N-depleted, N-P-depleted BG-11 medium. Long-term diazotrophic cultivation of A. variabilis IMU8 resulted in elevated PBP productivity with a limited impact on growth. When compared to N-depleted ones, N supply stimulated a slight induction… More >

Xihua Liu¹, Changjun Ding², Xiaohua Su^2,*

Phyton-International Journal of Experimental Botany, Vol.88, No.4, pp. 425-433, 2019, DOI:10.32604/phyton.2019.08285

Abstract The European black poplar (Populus nigra L.) has been used as a germplasm resource for the breeding of new poplar varieties around the world. The identification and screening of its high nitrogen use efficiency genotypes could enable the breeding of new resource-efficient poplar varieties. The accessions were screened using MALDI-TOF MS genotyping technology for ammonium transporter (AMT) and nitrate transporters (NRT) genes against phenotypic data for seedling height and ground diameter traits, in both low and high nitrogen environments. Allele re-sequencing of seven genes related to root development was carried out using the minisequencing method. By cluster analysis, 101 accessions… More >

MAURO RODRIGO SURENCISKI^*, EDUARDO ALBERTO FLACHSLAND, GRACIELA TERADA, LUIS AMADO MROGINSKI, HEBE YOLANDA REY

BIOCELL, Vol.36, No.1, pp. 31-36, 2012, DOI:10.32604/biocell.2012.36.031

Abstract The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen… More >

LUIS A. MROGINSKI*, PEDRO A. SANSBERRO, ADRIANA M. SCOCCHI, CLAUDIA LUNA, HEBE Y. REY

BIOCELL, Vol.32, No.1, pp. 33-39, 2008, DOI:10.32604/biocell.2008.32.033

Abstract Tropical Ilex species have recalcitrant seeds. This work describes experiments demonstrating the feasibility of long-term conservation of Ilex brasiliensis, I. brevicuspis, I. dumosa, I. intergerrima, I. paraguariensis, I. pseudoboxus, I. taubertiana, and I. theezans through cryopreservation of zygotic rudimentary embryos at the heart developmental stage. The embryos were aseptically removed from the seeds and precultured (7 days) in the dark, at 27± 2ºC on solidified (0.8% agar) 1/4MS medium, [consisting of quarterstrength salts and vitamins of Murashige and Skoog (1962) medium] with 3% sucrose and 0.1 mg/l Zeatin. The embryos were then encapsulated in 3% calcium alginate beads and pretreated… More >