XUHUI ZHAO^1,2, XIAOMIN HUANG¹, CHUNYAN DANG², XIA WANG¹, YUJIAO QI³, HONGLING LI^2,*

Oncology Research, Vol.32, No.5, pp. 999-1009, 2024, DOI:10.32604/or.2024.046679

Abstract Background: EBV-miR-BARTs exhibit significant relevance in epithelial tumors, particularly in EBV-associated gastric and nasopharyngeal cancers. However, their specific mechanisms in the initiation and progression of gastric cancer remain insufficiently explored. Material and Methods: Initially, EBV-miRNA-BART6-5p and its target gene SMAD4 expression were assessed in EBV-associated gastric cancer tissues and cell lines. Subsequent transfection induced overexpression of EBV-miRNA-BART6-5p in AGS and MKN-45, and downregulation in EBV-positive cells (SUN-719). The subsequent evaluation aimed to observe their impact on gastric cancer cell proliferation, migration, and glycolytic processes, with the TGF-β/SMAD4 signaling pathway value clarified using a TGF-β inhibitor. Results: EBV-miRNA-BART6-5p exhibits pronounced upregulation… More >

JINGJING TIAN^1,*, XIBAO HU², XINRONG ZHANG¹

Oncology Research, Vol.32, No.5, pp. 925-931, 2024, DOI:10.32604/or.2023.042447

Abstract To confirm the relationship between Circ_0003855 and EC, we purchased the Human esophageal carcinoma cell line Eca109 and normal human esophageal epithelial cells HEEC, and the expression levels of Circ_0003855, miR-622, and FLOT1 were detected. The results show that Circ_0003855 and FLOT1 were highly expressed in Eca109 cells, while miR-622 was lowly expressed (p < 0.05). Subsequently, Circ_0003855 small interfering RNA (si-Circ_0003855) and its negative control (si-NC) were used to detect changes in cellular biological behaviors. We found that the activity of Eca109 cells was reduced after interfering with the expression of Circ_0003855, and miR-622 expression was elevated, while FLOT1… More >

JIA SHAO^1,2, CAN ZHANG², YAONAN TANG², AIQIN HE², WEIPEI ZHU^1,*

BIOCELL, Vol.48, No.4, pp. 571-580, 2024, DOI:10.32604/biocell.2024.049092

Abstract Objective: Previous studies indicated that aberrant circular RNA (circRNA) expression affects gene expression regulatory networks, leading to the aberrant activation of tumor pathways and promoting tumor cell growth. However, the expression, clinical significance, and effects on cell propagation, invasion, and dissemination of circRNA_001896 in cervical cancer (CC) tissues remain unclear. Methods: The Gene Expression Omnibus (GEO) datasets (GSE113696 and GSE102686) were used to examine differential circRNA expression in CC and adjacent tissues. The expression of circRNA_001896 was detected in 72 CC patients using fluorescence quantitative PCR. Correlation analysis with clinical pathological features was performed through COX multivariate and univariate analysis.… More >

GUOQIANG HOU^1,2,#, XINHANG XU^2,#, WEIXING HU^1,*

Oncology Research, Vol.32, No.4, pp. 727-736, 2024, DOI:10.32604/or.2023.043391

Abstract Primary tumors of the central nervous system (CNS) are classified into over 100 different histological types. The most common type of glioma is derived from astrocytes, and the most invasive glioblastoma (WHO IV) accounts for over 57% of these tumors. Glioblastoma (GBM) is the most common and fatal tumor of the CNS, with strong growth and invasion capabilities, which makes complete surgical resection almost impossible. Despite various treatment methods such as surgery, radiotherapy, and chemotherapy, glioma is still an incurable disease, and the median survival time of patients with GBM is shorter than 15 months. Thus, molecular mechanisms of GBM… More >

JINNI MA^#, MEILIN ZHOU^#, XIN XU, XINYAO GAO, HAIXIA WANG, JINHUA SHEN, LU XUE^*

BIOCELL, Vol.48, No.2, pp. 239-252, 2024, DOI:10.32604/biocell.2023.045076

Abstract Background: Placenta specific 8 (PLAC8) is a candidate oncogene involved in the development and progression of solid tumors. However, the status of PLAC8 in lung cancer (LC), especially non-small cell lung cancer (NSCLC) is still not lucid. Methods: Tissue microarray analysis (TMA) was performed to detect the expression patterns of PLAC8 in LC tissues and cell lines. Then a series of cellular experiments were performed fto assess cell proliferation, cell cycle profiles, and cell motility to explore the role of PLAC8 in NSCLC-derived cell lines: H1299 and A549. Results: TMA results showed that PLAC8 played complex and even contradictory roles… More >

HUI SUN, MASANORI KAWANO^*, TATSUYA IWASAKI, ICHIRO ITONAGA, YUTA KUBOTA, HIROSHI TSUMURA, KAZUHIRO TANAKA

Oncology Research, Vol.32, No.3, pp. 463-476, 2024, DOI:10.32604/or.2023.044085

Abstract An important factor in the emergence and progression of osteosarcoma (OS) is the dysregulated expression of microRNAs (miRNAs). Transcription factor 7-like 1 (TCF7L1), a member of the T cell factor/lymphoid enhancer factor (TCF/LEF) transcription factor family, interacts with the Wnt signaling pathway regulator β-catenin and acts as a DNA-specific binding protein. This study sought to elucidate the impact of the interaction between miR-329-3p and TCF7L1 on the growth and apoptosis of OS and analyze the regulatory expression relationship between miRNA and mRNA in osteosarcoma cells using a variety of approaches. MiR329-3p was significantly downregulated, while TCF7L1 was considerably up-regulated in… More > Graphic Abstract

DONGQIN WANG^1,4, YAN SHI¹, ZHIQIANG WANG⁴, JING ZHANG², LUYAO WANG², HONGYU MA³, SHUHUA SHI², XIAOFU LIAN², HUA HUANG⁴, XIAOJING WANG^1,*, CHAOQUN LIAN^4,*

BIOCELL, Vol.48, No.1, pp. 111-122, 2024, DOI:10.32604/biocell.2023.046903

Abstract Introduction: Among all malignant tumors of the digestive system, pancreatic carcinoma exhibits the highest mortality rate. Currently, prevention and effective treatment are urgent issues that need to be addressed. Methods: The study focused on meiotic nuclear divisions 1 (MND1), integrating data from the Gene Expression Profiling Interactive Analysis (GEPIA) database with prognostic survival analysis. Simultaneously, experiments at cellular level were employed to demonstrate the effect of MND1 on the proliferation and migration of PC. The small-molecule inhibitor of MND1 was used to suppress the migration of PC cells by knocking down MND1 using small interfering RNA (siRNA) in Patu-8988 and… More >

CHONG SHEN, JIAJUN YAN^*, YU REN, ZHIRONG ZHU, XIAOLONG ZHANG, SHUIXIANG TAO

BIOCELL, Vol.48, No.1, pp. 97-109, 2024, DOI:10.32604/biocell.2023.045303

Abstract Introduction: Bladder cancer (BC) has a high incidence and mortality rate worldwide. Suppressor anaphase-promoting complex domain containing 2 (SAPCDC2) is over-expressed in a variety of tumors. Objectives: This study investigated the effects of SAPCD2 knockdown on BC cells. Methods: T24 and UMUC3 cell models and the xenografted BC tumor model with SAPCD2 knockdown were established to observe the malignant phenotype of BC cells by cell counting kit-8 assay, colony formation test, wound healing, and Transwell assay, mRNA and proteins expressions were measured with quantitative real-time polymerase chain reaction, western blotting, and tissue immunohistochemistry. Lithium chloride agonist on the Wnt/β-catenin pathway… More > Graphic Abstract

MINJI PARK¹, CHULHWAN BANG², WON-SOO YUN³, YUN-MI JEONG^3,*

Oncology Research, Vol.32, No.2, pp. 273-282, 2024, DOI:10.32604/or.2023.044362

Abstract Fucoidan, a sulfate polysaccharide obtained from brown seaweed, has various bioactive properties, including anti-inflammatory, anti-cancer, anti-viral, anti-oxidant, anti-coagulant, anti-thrombotic, anti-angiogenic, and anti-Helicobacter pylori properties. However, the effects of low-molecular-weight fucoidan (LMW-F) on melanoma cell lines and three dimensional (3D) cell culture models are not well understood. This study aimed to investigate the effects of LMW-F on A375 human melanoma cells and cryopreserved biospecimens derived from patients with advanced melanoma. Ultrasonic wave was used to fragment fucoidan derived from Fucus vesiculosus into smaller LMW-F. MTT and live/dead assays showed that LMW-F inhibited cell proliferation in both A375 cells and patient-derived melanoma… More >

TINGTING LI¹, WEI ZHONG¹, LIU YANG¹, ZHIYU ZHAO¹, LI WANG¹, CONG LIU¹, WANYUN LI¹, HAIYAN LV², SHENGYU WANG¹, JIANGHUA YAN¹, TING WU^1,*, GANG SONG^1,*, FANGHONG LUO^1,*

Oncology Research, Vol.32, No.2, pp. 361-371, 2024, DOI:10.32604/or.2023.043807

Abstract The high mortality rate associated with gastric cancer (GC) has resulted in an urgent need to identify novel therapeutic targets for GC. This study aimed to investigate whether GAIP interacting protein, C terminus 1 (GIPC1) represents a therapeutic target and its regulating mechanism in GC. GIPC1 expression was elevated in GC tissues, liver metastasis tissues, and lymph node metastases. GIPC1 knockdown or GIPC1 blocking peptide blocked the platelet-derived growth factor receptor (PDGFR)/PI3K/AKT signaling pathway, and inhibited the proliferation and migration of GC cells. Conversely, GIPC1 overexpression markedly activated the PDGFR/PI3K/AKT signaling pathway, and promoted GC cell proliferation and migration. Furthermore,… More > Graphic Abstract