Cerna¹ E, Y Ochoa², R Mendoza¹, MH Badii³, G Gallegos¹, J Landeros¹

Phyton-International Journal of Experimental Botany, Vol.79, pp. 147-152, 2010, DOI:10.32604/phyton.2010.79.147

Abstract Protein assays were conducted on Tetranychus urticae Koch, as potential resistance detection tools to plaguicides. This is a phytophagous mite that feeds on a large variety of plants. Experiments were carried out using a pesticide susceptible and three field crop strains of T. urticae. Protein was measured by colorimetric assays, using Kit-II from Bio-Rad, with bovine serum albumin (BSA) as standard. Homogenates were prepared using 10, 30, 50, 100, 300, 500 and 800 mites, with 30 replicates each. Linearity was obtained for the standard curve of the different methods, and r² values ranged from 0.877 to 0.985. The Bradford method… More >

Bonamico¹ NC, MG Balzarini², AT Arroyo², MA Ibañez¹, DG Díaz³, JC Salerno³, MA Di Renzo¹

Phyton-International Journal of Experimental Botany, Vol.79, pp. 31-38, 2010, DOI:10.32604/phyton.2010.79.031

Abstract Resistance to Mal de Río Cuarto (MRC) disease in maize (Zea mays L.) is important in Argentina because the crop area involves a wide region where the disease is endemic. Molecular marker-assisted selection could be used as an additional selection tool to enhance precision of the genotype selection for resistance. It demands the identification of informative markers. Microsatellite (SSR) markers linked to gene(s) associated with resistance to MRC have been reported from previous QTL analyses. These analyses have been made on linkage maps derived from a relatively early mapping population. In advanced populations, where highly distinct genotypes are easily classified,… More >

Giménez¹ C, E de García², O Haddad³

Phyton-International Journal of Experimental Botany, Vol.77, pp. 65-79, 2008, DOI:10.32604/phyton.2008.77.065

Abstract Evaluation of clonal micropropagation and phenotype stability of elite somaclones are critical steps for development of new varieties. In the present work somaclon variant CIEN BTA-03 (resistant to Black Sigatoka), obtained through in vitro process from cultivar Williams (susceptible to Black Sigatoka), was micropropagated via apical shoot culture for five multiplication cycles in 0.5 mg/l of benzyl-aminopurine (BA). To verify the genetic stability of the progeny of this elite material, random amplified polymorphic DNA (RAPD) markers were used. A total of 5,292 monomorphic bands were obtained from the amplification of fifty six DNA samples (extracted from vitroplants randomly selected) with… More >

T.H. Satoh², T.A. Surmacz³, O. Nyormoi⁴, C.M. Whitacre¹

BIOCELL, Vol.27, No.1, pp. 47-55, 2003, DOI:10.32604/biocell.2003.27.047

Abstract This study shows a strong association between cell attachment to substratum and activation of β1-integrin-signaling with resistance to the camptothecin derivative topotecan (TPT) in breast cancer cells. We propose a mechanistic-driven approach to sensitize the cells to camptothecins. ZR-75-1 anchoragedependent breast cancer cell line, its derivative 9D3S suspension cells (9D3S-S), and 9D3S cells attached to fibronectin-coated plates (9D3S-A) were treated with TPT (1 µM) or CPT-11 (40 µM) for 48 h. Programmed cell death (PCD), as shown by poly(ADP-ribose) polymerase (PARP), pro-caspase-3 and pro-caspase-9 cleavage, was observed in 9D3S-S cells but not in ZR-75-1 or 9D3S-A cells. Because p125 focal… More >

Guozhong Dai^1,*, Jia Zhu², Guicai Shi³, Yanmin Sheng⁴, Shujin Li⁵

Structural Durability & Health Monitoring, Vol.11, No.2, pp. 169-190, 2017, DOI:10.3970/sdhm.2017.011.169

Abstract As the landfill leachate has strong pollution on the underground water, surface water and soil. This paper develops the formula of impervious slurry with low permeability, good durability, strong adsorption and retardant based on the bentonite which is modified by polyvinyl alcohol. Through the simulation experiment, the optimum formula of polyvinyl alcohol is 0.2%. Its osmotic coefficient for 28 days is 0.53×10-8~1.86×10-8 cm/s and compressive strength is 0.5~1.5 MPa as well. This paper study on the retardant rule of the consolidation of slurry against the pollution in the leachate by self-made percolation instrument. The experiment shows that the retardant rate… More >

LUCIA PRONSATO, ANABELA LA COLLA, ANA CAROLINA RONDA, LORENA MILANESI, RICARDO BOLAND, ANDREA VASCONSUELO^*

BIOCELL, Vol.37, No.1, pp. 1-9, 2013, DOI:10.32604/biocell.2013.37.001

Abstract Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as lC2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2 O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2 O2 induction of apoptosis. We propose serially cultured C2C12… More >

F. Balle¹, S. Huxhold¹, G. Wagner¹, D. Eifler¹

Structural Durability & Health Monitoring, Vol.8, No.4, pp. 359-370, 2012, DOI:10.32604/sdhm.2012.008.359

Abstract Aluminum alloys and carbon fiber reinforced polymers (CFRP) are two important materials for lightweight design and the combination of these dissimilar materials becomes increasingly important. Recent investigations have shown that ultrasonic metal welding is a well suited process to realize aluminum/CFRP-joints.The ultrasonic shear oscillation parallel to the welding zone with a simultaneous welding force perpendicular to the aluminum/CFRP-sheets melts the polymer matrix and squeezes the polymer matrix out of the welding zone. This allows a direct contact between the carbon fibers and the aluminum. Beside monotonic properties the cyclic deformation behavior of these ultrasonically welded aluminum/CFRP-joints is essential. Therefore in… More >

I. Černý^1,2, D. Mikulová¹

Structural Durability & Health Monitoring, Vol.6, No.1, pp. 43-52, 2010, DOI:10.3970/sdhm.2010.006.043

Abstract Alternative methods of material machining like electro-erosive or water jet cutting, respectively, represent modern technologies, which are perspective to be used as final end-to-shape operations due to their possibilities of automatization and cutting precision. The paper contains results of an investigation of resistance of an aircraft AISI 4340 steel against fatigue loading performed using specimens loaded by three point bending at ambient temperature and standard laboratory conditions. Results of fatigue tests of specimens with surface after electro-erosive and water jet cutting, respectively, are presented, whereas in the latter case, areas of water jet incidence and outfall are studied separately. The… More >

S. Baragetti¹, A. Terranova²

Structural Durability & Health Monitoring, Vol.1, No.4, pp. 267-276, 2005, DOI:10.3970/sdhm.2005.001.267

Abstract Aim of this work is to investigate into the possibility of enhancing the fatigue resistance of CrN PVD coated components. In particular PVD coated spur gears were tested and numerical simulation of crack propagation was carried out. The coating layer micro-hardness and the residual stresses characterising the surface film were measured and the obtained results were introduced in a numerical modelling predicting fatigue life procedure of coated gears used in gearboxes for automotive applications. The number of cycles necessary to reach specified crack depths of coated and uncoated samples was numerically determined and represents a powerful tool to predict fatigue… More >

L. Fratini¹, S. Pasta²

Structural Durability & Health Monitoring, Vol.1, No.4, pp. 245-252, 2005, DOI:10.3970/sdhm.2005.001.245

Abstract In the last years friction stir welding (FSW) has reached a quite large diffusion in the welding of aluminium alloys, difficult to be welded with traditional technologies. The objective of this investigation was to investigate the influence of FSW process parameters on the fatigue strength of the developed joints. Moreover, in order to improvement the strength of joint, the effect of a post-welding treatment has been highlighted; what is more a surface finish treatment has been developed with the aim to eliminate the stress concentration caused by welding process on the surface of the joints. Finally, the fracture locations have… More >