Ming Dong^#, Yan Wang^#, Shulin Wang, Guoyi Feng, Qian Zhang, Yongzeng Lin, Qinglong Liang, Yongqiang Wang^*, Hong Qi^*

Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2841-2860, 2023, DOI:10.32604/phyton.2023.030465

Abstract Restructuring tilth layers (RTL) is a tillage method that exchanges the 0–20 and 20–40 cm soil layers that can be applied during cotton cultivation to increase cotton yield, eliminate weeds and alleviate severe disease, including Verticillium wilt. However, the mechanism by which RTL inhibits Verticillium wilt is unclear. Therefore, we investigated the distribution of microbial communities after rotary tillage (CK) and RTL treatments to identify the reasons for the reduction of Verticillium wilt in cotton fields subjected to RTL. Illumina high-throughput sequencing was used to sequence the bacterial and fungal genes. The disease incidence and severity of Verticillium wilt decreased… More >

Liyan Wu¹, Jie Cheng^1,2, Yaju Gong¹, Rui Bao¹, Zhibin Li¹, Min Gui^1,*, Guanghui Du^2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 591-609, 2023, DOI:10.32604/phyton.2022.023481

Abstract In this study, wild eggplant germplasm No. M239, which is highly susceptible to Verticillium wilt, was used as the experimental material. The physiological and biochemical indices (SOD, PAL, MDA and soluble protein) of M239 roots were measured at different times (0, 12, 24, 36, 48, 60 and 72 h) post inoculation with Verticillium dahliae, and the key time points for the M239 response to Verticillium wilt infection were screened. Then, RNA-Seq technology was used to screen the differentially expressed genes (DEGs) in M239 roots at 0, 12 and 48 h post-inoculation (hpi). The transcriptional results of M239 were also compared… More >

Chengyang Li^1,2,#, Lianjun Wang^1,#, Shasha Chai^1,#, Yinghua Xu^1,2, Chong Wang^1,2, Yi Liu^1,2, Jian Lei¹, Xiaojie Jin¹, Xianliang Cheng¹, Yuanyuan Yang¹, Xinsun Yang^1,*, Wenying Zhang^2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1779-1793, 2022, DOI:10.32604/phyton.2022.020192

Abstract Fusarium wilt, a disease caused by Fusarium oxysporum f.sp batatas (Fob) is an important disease in sweet potato production. Using endophytic bacteria for biological control of sweet potato diseases is one of the important ways. A Bacillus subtilis with antagonistic effect on Fusarium wilt of sweet potato was isolated from soil by confrontation culture. According to the biological characteristics, 16S rDNA sequence analysis, and physiological and biochemical analysis, the Bacillus subtilis HAAS01 was named. A pot experiment was conducted for the biological control experiment of strain HAAS01, and the endogenous hormone content, antioxidant enzyme activity, soluble protein content, and related… More >

Ali Raza^1,*, Emad E. Mahmoud², A. M. Al-Bugami², Dumitru Baleanu^3,4, Muhammad Rafiq⁵, Muhammad Mohsin⁶, Muneerah Al Nuwairan⁷

CMC-Computers, Materials & Continua, Vol.71, No.3, pp. 5293-5310, 2022, DOI:10.32604/cmc.2022.024535

Abstract Pine wilt is a dramatic disease that kills infected trees within a few weeks to a few months. The cause is the pathogen Pinewood Nematode. Most plant-parasitic nematodes are attached to plant roots, but pinewood nematodes are found in the tops of trees. Nematodes kill the tree by feeding the cells around the resin ducts. The modeling of a pine wilt disease is based on six compartments, including three for plants (susceptible trees, exposed trees, and infected trees) and the other for the beetles (susceptible beetles, exposed beetles, and infected beetles). The deterministic modeling, along with subpopulations, is based on… More >

Fatih M. Tok¹, Sibel Dervis^2,*, Halit Yetisir³

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 303-314, 2020, DOI:10.32604/phyton.2020.08801

Abstract Forty-four V. dahliae isolates were collected from symptomatic vascular tissues of okra plants each from a different field in eight provinces located in the eastern Mediterranean and western Anatolia regions of Turkey during 2006- 2009. Nitrate-nonutilizing (nit) mutants of V. dahliae from okra were used to determine heterokaryosis and genetic relatedness among isolates. All isolates from okra plants were grouped into two vegetative compatibility groups (VCGs) (1 and 2) and three subgroups as 1A (13.6%, 6/44), 2A (20.5%, 9/44) and 2B (65.9%, 29/44) according to international criteria. Pathogenicity tests were performed on a susceptible local okra (A. esculentus) landrace in… More >

Kiehr M¹, R Delhey¹, J Lusto²

Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 423-426, 2015, DOI:10.32604/phyton.2015.84.423

Abstract A new disease of lettuce has been observed in the surroundings of Bahía Blanca, Argentina. The symptoms include dwarfing, general chlorosis, wilting, root rot, and leaf blight, sometimes followed by plant death. Pythium tracheiphilum Matta was found to be consistently associated with those symptoms. The morphological and morphometric characteristics of this oomycete are described. Isolates obtained from field-infected lettuce plants were inoculated to lettuce and caused similar symptoms as those found in natural infections. Seedling emergence was also severely affected following experimental inoculation. Koch’s Postulates were fulfilled by recovering the fungus from inoculated plants. The new disease shows a high… More >

Mora-Silva W^1,3, FR Garcés-Fiallos^1,2, C Suarez-Capello^1,2, CE Belezaca-Pinargote^1,3, P Cedeño-Loja¹, E Vallejo³

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 120-127, 2015, DOI:10.32604/phyton.2015.84.120

Abstract In the Ecuadorian coast one of the most destructive diseases of the pachaco is vascular wilt or stem rot caused by Ceratocystis complex, so the aim of this study was to determine the factors that affect the efficiency of the reaction of bark pachaco to this disease. This research was conducted under laboratory conditions, using trees pachaco S38, S41, S98, AE-1, AE-2 and AE-3, and pathogenic species Ceratocystis paradoxa and C. moniliformis. The method utilized was tissue stem bark,with bark sections with 4.5 cm², and a suspension of 3x10⁴ units infection and remained in a humid chamber for 96 hours… More >

Hernández-Castillo FD¹, RH Lira-Saldivar², G Gallegos-Morales¹, M Hernández-Suárez¹, S Solis-Gaona²

Phyton-International Journal of Experimental Botany, Vol.83, pp. 49-55, 2014, DOI:10.32604/phyton.2014.83.049

Abstract One of the most severe phytosanitary problems that face chili pepper producers in Mexico, and in many other parts of the world, is the disease known as "secadera" or wilting, caused by diverse pathogens. These patogens are mainly controlled with synthetic pesticides, thus causing a severe ecological impact, toxicity to humans, generation of plant resistance to fungicides, and increments of production costs. Because of this, it rises the need of finding more environmentally friendly options. We evaluated rhizospheric bacteria as a possible biological control of pepper wilt. We used three bacterial strains belonging to the Bacillus genera. These strains were… More >

Barocio-Ceja NB¹, LF Ceja-Torres^1*, JL Morales-García², HV Silva-Rojas³, R Flores-Magallón¹, S Ochoa-Estradav¹

Phyton-International Journal of Experimental Botany, Vol.82, pp. 15-22, 2013, DOI:10.32604/phyton.2013.82.015

Abstract The objectives of this study were to (1) isolate and identify pathogenic fungi from vegetative material with wilt symptoms in tomato plantations belonging to the Cienega of Chapala, Michoacán, Mexico, and (2) determine the antagonistic capacity of Trichoderma sp. and Aspergillus spp. isolated from chicken-manure vermicompost. Pathogens were isolated by means of a completely randomized sampling in 6 locations; 9 plantations were inspected and 45 plants with symptoms of the disease were selected. Portions of root and stem were disinfected and placed on potato-dextroseagar acidified (PDA). Antagonists isolation was made from a dilution of chicken-manure vermicompost of 1 x 10^-2… More >

García-Munguía CA¹, M Acosta-Ramos², O Vázquez-Martinez³, M Pérez-Sato⁵, A García-Munguía⁴, O García-Munguía⁶, AM García-Munguía³

Phyton-International Journal of Experimental Botany, Vol.86, pp. 258-264, 2017, DOI:10.32604/phyton.2017.86.258

Abstract The illnesses that cause root and neck rot in golf courses constitute a limiting factor for grass development worldwide. This is mainly true when management guidelines are not conducted. Our research was conducted in the Golf Club “Mexico”, located in Mexico, D.F. Isolates were taken from plants that presented yellowing symptoms and root and neck rot. The identified pathogenic fungi, isolated from axenic media, were Giberella avenacea, Fusarium oxysporum, F. oxysporum f. sp. cubense and Pythium ultimum. The molecular characterization showed a comparison of the DNA-obtained sequences which was morphologically determined as F. avenaceum and was molecularly corroborated as G.… More >